A Couple’s Marital Disharmony and its Psychological Effects on their Children during the HIV Disclosure Process in Kenya

Limited published data exists on how HIV-positive parents perform disclosure to all their children. A couple’s HIV disclosure experience to all their children is presented. They participated in a larger study conducted to understand the lived experiences of HIV-positive parents and their children during the disclosure process. Each underwent individualized in-depth semi-structured interviews. Interviews were transcribed and transferred into NVivo 8 for analysis using the Van Kaam method. Three themes emerged including HIV testing, full disclosure delivery accompanied by marital disharmony, and post-disclosure psychological effects on the family. Marital disharmony and non-involvement of the father caused the mother to fully disclose their illnesses to their four oldest children. All children were affected by disclosure, one had a delayed emotional outburst, and another was still angry and withdrawn years later. HIV-positive parents with poor relationships within the families need intense counseling and support pre, during, and post-disclosure to improve outcomes.

Demonstrating PM2.5 and road-side dust pollution by heavy metals along Thika superhighway in Kenya, sub-Saharan Africa

This study assessed the level of heavy metal in roadside dust and PM2.5 mass concentrations along Thika superhighway in Kenya. Thika superhighway is one of the busiest roads in Kenya, linking Thika town with Nairobi. Triplicate road dust samples collected from 12 locations were analysed for lead (Pb), chromium (Cr), cadmium (Cd), nickel (Ni), zinc (Zn), and copper (Cu) using atomic absorption spectrophotometry (AAS). PM2.5 samples were collected on pre-weighed Teflon filters using a BGI personal sampler and the filters were then reweighed. The ranges of metal concentrations were 39–101 μg/g for Cu, 95–262 μg/g for Zn, 9–28 μg/g for Cd, 14–24 μg/g for Ni, 13–30 μg/g for Cr, and 20–80 μg/g for Pb. The concentrations of heavy metals were generally highly correlated, indicating a common anthropogenic source of the pollutants. The results showed that the majority of the measured heavy metals were above the background concentration, and in particular, Cd, Pb, and Zn levels indicated moderate to high contamination. Though not directly comparable due to different sampling timeframes (8 h in this study and 24 h for guideline values), PM2.5 for all sites exceeds the daily WHO PM2.5 guidelines of 25 μg/m3. This poses a health risk to people using and working close to Thika superhighway, for example, local residents, traffic police, street vendors, and people operating small businesses. PM2.5 levels were higher for sites closer to Nairobi which could be attributed to increased vehicular traffic towards Nairobi from Thika. This study provides some evidence of the air pollution problem arising from vehicular traffic in developing parts of the world and gives an indication of the potential health impacts. It also highlights the need for source apportionment studies to determine contributions of anthropogenic emissions to air pollution, as well as long-term sampling studies that can be used to fully understand spatiotemporal patterns in air pollution within developing regions

A thermostable presentation of the live, attenuated peste des petits ruminants vaccine in use in Africa and Asia

The research objective was to develop a thermostable vaccine against peste des petits ruminants (PPR), a morbilliviral disease of small ruminants targeted for eradication that is a major constraint on the livelihoods of the rural poor throughout much of Africa and Asia. Although existing PPR vaccines provide life-long immunity, they require continuous refrigeration. This limits their utility in developing countries. Methods for the lyophilization of a related morbillivirus, rinderpest (RP), resulted in vaccine that could be used in the field for up to 30 days without refrigeration which was a major contribution to the global eradication of RP completed in 2011. The present research applied the rinderpest lyophilization method to the attenuated Nigeria 75/1 PPR vaccine strain, and measured thermostability in accelerated stability tests (AST) at 37 °C. The shelf-life of the vaccine was determined as the time a vial retained the minimum dose required as a 25-dose presentation at the specified temperature. A lactalbumin hydrolysate and sucrose (LS) stabilizer was compared to stabilizers based on trehalose. PPR vaccine produced using the Xerovac drying method was compared to vaccine produced using the rinderpest lyophilization method in AST. LS vaccine was evaluated in AST at 37, 45 and 56 °C and an Arrhenius plot was constructed for estimation of stability at temperatures not tested. Vaccines produced using LS and the rinderpest method of lyophilization were the most stable. The shelf-life of the Xerovac preparation was 22.2 days at 37 °C. The three LS vaccine batches had shelf-lives at 37 °C of 177.6, 105.0 and 148.9 days, respectively, at 37 °C. At 56 °C, the shelf-life was 13.7 days. The projected half-life at 25 °C was 1.3 years. This is sufficient thermostability for use without a cold chain for up to 30 days which will greatly facilitate the delivery of vaccination in the global eradication of PPR

Direct Detection of Triterpenoid Saponins in Medicinal Plants

Direct detection of saponins in medicinal plants using Fourier Transform Infrared (FTIR) spectroscopy is reported in this paper. Crude dry plant powders were mixed with potassium bromide (KBr) powder and compressed to a thin pellet for infrared examination. FTIR spectra of the test samples showed -OH, -C=O, C-H, and C=C absorptions characteristic of oleanane triterpenoid saponins. The C-O-C absorptions indicated glycoside linkages to the sapogenins. Phytochemical analysis confirmed the presence of saponins in the tested specimens. Entada leptostachya was used as a reference sample. Dry plant powder was extracted sequentially with hexane, dichloromethane, ethyl acetate and methanol. FTIR spectra of the reference sample powder and its organic solvent extracts showed characteristic saponin absorption peaks. These results indicated that direct detection of saponins in medicinal plants was possible by infrared analysis. Lengthy exhaustive chemical analyses necessary for detection of saponins could be avoided

Antimicrobial Activity of Some Medicinal Plants Used by Herbalists in Eastern Province, Kenya

The aqueous extracts from medicinal plants commonly used by herbalists in Mbeere, and Embu districts of Eastern province, Kenya, were tested for their inhibitory activity against three selected strains of bacteria. All the selected plant extracts (infusions: 1.0g sample in 100 ml water) investigated showed activity against Escherichia coli with inhibition zone diameters ranging from 5.8 – 18.0 mm. Terminalia brownii gave the largest inhibition zones against E. coli and Staphylococcus aureus. Vernonia lasiopus and Tithonia diversifolia were inactive to S. aureus and Bacillus subtilis, respectively. Eighteen and sixteen plants showed sensitivity of greater than 10 mm against S. aureus and B. subtilis, respectively. All control discs gave zones of inhibition of 12 – 24 mm, which were larger than those of the extracts. The present study validated the use of the selected medicinal plants by the herbalists in the treatment of bacterial ailments caused by the strains of bacteria investigated. Medicinal plants used for non-bacterial diseases also exhibited sensitivity towards bacterial strains tested. This implied they could be used as multi-purpose medicinal plants

Molecular characterization of live Theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity

The current Infection and Treatment Method of vaccination against East Coast fever comprises an inoculation of live Theileria parva sporozoites and simultaneous administration of oxytetracycline. Immunization with a combination of parasite types has been shown to provide broader protection than inoculation of individual strains. In this study, we used a high-throughput capillary electrophoresis system to determine the genotypic composition of the Muguga Cocktail, a widely used vaccine stabilate derived from three seed stabilates - Muguga, Serengeti-transformed and Kiambu 5. Five satellite markers were used to genotype the vaccine and reference stabilates from two commercial-scale preparations of the vaccine. In addition, 224 cloned cell lines established by infection of bovine lymphocytes with T. parva parasites from the component stabilates were genotyped. The results indicate that, for the recently prepared batch, there are at least eight genotypes in each of the Muguga and the Serengeti-transformed stabilates, whilst parasites from the Kiambu 5 stabilate showed no diversity at the five loci. The Serengeti-transformed stabilate contained parasites of the Kiambu 5 genotype and of two genotypes present in the Muguga stabilate, whereas there were no genotypes common to the Muguga and Kiambu 5 stabilates. When stabilates from the two vaccine batches were compared, no allelic variations were identified between the Muguga and Kiambu 5 parasites, whilst lack of sufficient clones prevented a full comparison of the Serengeti-transformed stabilates. The findings will facilitate examination of the extent to which the vaccine strains become resident in areas under vaccination, the identification of ‘breakthrough’ strains and the establishment of the quality assurance protocols to detect variations in the production of the vaccine. The cloned cell lines will be useful for further understanding the antigenic diversity of parasites in the vaccine

Extensive genotypic diversity in the live sporozoite East Coast Fever (ITM) vaccine

A poster prepared for the BecA launch, Nairobi, 5 November 2010

Traditional Medicines Among the Embu and Mbeere Peoples of Kenya

Ethnobotanical information and traditional medicines were investigated and documented in Embu and Mbeere districts, Eastern Province of Kenya. Oral interviews were obtained from over 100 herbalists, both men and women aged between 40 and 80 years. All the herbalists interviewed were Christians and had little formal education. Non-Christian herbalists were purported to combine herbal medicines with witchcraft and were not interviewed. Of the 40 commonly used herbal plants 25 were used as multi-purpose medicinal plants (mpmp), while 15 were used to treat one disease type. There was a correlation between the outpatient morbidity data at the local District hospital, and the common incident diseases treated by the herbalists. Generally a decoction or infusion of the herb was recommended for the treatment of internal or external condition of the patients. Malaria and typhoid were treatable with a total of 15 and 12 plants respectively and were among the first two commonest diseases found in the study area. Terminalia brownii was found to be the most used medicinal plant either alone or in combination with other herbs. The second and third most utilized medicinal plants were Ovariodendron anisatum and Wurbugia ugadensis respectively