Topics concerning state variable feedback in automatic control systems. Part 1 - Specification. Part 2 - Sensitivity. Part 3 - Intentional nonlinearities. Part 4 - Unavailable states

Specifications, sensitivity, intentional nonlinearities, and unavailable states concerned with state variable feedback in automatic control system

Search for Mars lander/rover/sample-return sites: A status review

Ten Mars sites were studied in the USA for four years. The sites are the Chasma Boreale (North Pole), Planum Australe (South Pole), Olympus Rupes, Mangala Valles, Memnonia Sulci, Candor Chasma, Kasel Valles, Nilosyrtis Mensae, Elysium Montes, and Apollinaris Patera. Seven sites are being studied by the USSR; their prime sites are located at the east mouth of Kasel Valles and near Uranius Patera. Thirteen geological maps of the first six USA sites are compiled and in review. Maps of the Mangala East and West sites at 1:1/2 million scale and a 1:2 million scale map show evidence of three episodes of small-channel formation interspersed with episodes of volcanism and tectonism that span the period from 3.5 to 0.6 b.y. ago. The tectonic and geological history of Mars, both ancient and modern, can be elucidated by sampling volcanic and fluvial geologic units at equatorial sites and layered deposits at polar sites. The evidence appears clear for multiple episodes of fluvial channeling, including some that are quite recent; this evidence contrasts with the theses of Baker and Partridge (1986) and many others that all channels are ancient. Verification of this hypothesis by Mars Observer will be an important step forward in the perception of the history of Mars

Effect of Oscillating Landau Bandwidth on the Integer Quantum Hall Effect in a Unidirectional Lateral Superlattice

We have measured activation gaps for odd-integer quantum Hall states in a unidirectional lateral superlattice (ULSL) -- a two-dimensional electron gas (2DEG) subjected to a unidirectional periodic modulation of the electrostatic potential. By comparing the activation gaps with those simultaneously measured in the adjacent section of the same 2DEG sample without modulation, we find that the gaps are reduced in the ULSL by an amount corresponding to the width acquired by the Landau levels through the introduction of the modulation. The decrement of the activation gap varies with the magnetic field following the variation of the Landau bandwidth due to the commensurability effect. Notably, the decrement vanishes at the flat band conditions.Comment: 7 pages, 6 figures, minor revisio

Imaging Electronic Correlations in Twisted Bilayer Graphene near the Magic Angle

Twisted bilayer graphene with a twist angle of around 1.1{\deg} features a pair of isolated flat electronic bands and forms a strongly correlated electronic platform. Here, we use scanning tunneling microscopy to probe local properties of highly tunable twisted bilayer graphene devices and show that the flat bands strongly deform when aligned with the Fermi level. At half filling of the bands, we observe the development of gaps originating from correlated insulating states. Near charge neutrality, we find a previously unidentified correlated regime featuring a substantially enhanced flat band splitting that we describe within a microscopic model predicting a strong tendency towards nematic ordering. Our results provide insights into symmetry breaking correlation effects and highlight the importance of electronic interactions for all filling factors in twisted bilayer graphene.Comment: Main text 9 pages, 4 figures; Supplementary Information 25 page

Scaling properties of protein family phylogenies

One of the classical questions in evolutionary biology is how evolutionary processes are coupled at the gene and species level. With this motivation, we compare the topological properties (mainly the depth scaling, as a characterization of balance) of a large set of protein phylogenies with a set of species phylogenies. The comparative analysis shows that both sets of phylogenies share remarkably similar scaling behavior, suggesting the universality of branching rules and of the evolutionary processes that drive biological diversification from gene to species level. In order to explain such generality, we propose a simple model which allows us to estimate the proportion of evolvability/robustness needed to approximate the scaling behavior observed in the phylogenies, highlighting the relevance of the robustness of a biological system (species or protein) in the scaling properties of the phylogenetic trees. Thus, the rules that govern the incapability of a biological system to diversify are equally relevant both at the gene and at the species level.Comment: Replaced with final published versio

How Many Species Are There on Earth and in the Ocean?

The diversity of life is one of the most striking aspects of our planet; hence knowing how many species inhabit Earth is among the most fundamental questions in science. Yet the answer to this question remains enigmatic, as efforts to sample the world's biodiversity to date have been limited and thus have precluded direct quantification of global species richness, and because indirect estimates rely on assumptions that have proven highly controversial. Here we show that the higher taxonomic classification of species (i.e., the assignment of species to phylum, class, order, family, and genus) follows a consistent and predictable pattern from which the total number of species in a taxonomic group can be estimated. This approach was validated against well-known taxa, and when applied to all domains of life, it predicts ∼8.7 million (±1.3 million SE) eukaryotic species globally, of which ∼2.2 million (±0.18 million SE) are marine. In spite of 250 years of taxonomic classification and over 1.2 million species already catalogued in a central database, our results suggest that some 86% of existing species on Earth and 91% of species in the ocean still await description. Renewed interest in further exploration and taxonomy is required if this significant gap in our knowledge of life on Earth is to be closed

Structural Conformers of (1,3-Dithiol-2-ylidene)ethanethioamides: The Balance Between Thioamide Rotation and Preservation of Classical Sulfur-Sulfur Hypervalent Bonds

The reaction of N-(2-phthalimidoethyl)-N-alkylisopropylamines and S2Cl2 gave 4-N-(2-phthalimidoethyl)-N-alkylamino-5-chloro-1,2-dithiol-3-thiones that quantitatively cycloadded to dimethyl or diethyl acetylenedicarboxylate to give stable thioacid chlorides, which in turn reacted with one equivalent of aniline or a thiole to give thioanilides or a dithioester. Several compounds of this series showed atropisomers that were studied by a combination of dynamic NMR, simulation of the signals, conformational analysis by DFT methods, and single crystal X-ray diffraction, showing a good correlation between the theoretical calculations, the experimental values of energies, and the preferred conformations in the solid state. The steric hindering of the crowded substitution at the central amine group was found to be the reason for the presence of permanent atropisomers in this series of compounds and the cause of a unique disposition of the thioxo group at close-to-right angles with respect to the plane defined by the 1,3-dithiole ring in the dithiafulvene derivatives, thus breaking the sulfur–sulfur hypervalent bond that is always found in this kind of compounds.Ministerio de Economıá y Competitividad, Spain (Project CTQ2012- 31611), Junta de Castilla y León, Consejería de Educación y Cultura y Fondo Social Europeo (Project BU246A12-1), and the European Commission, Seventh Framework Programme (Project SNIFFER FP7-SEC-2012-312411

Cambridge Monographs in Experimental Biology

and the magnitude of FCT. Because active torque is proportional to n 2 and passive torque to n, the ratio of active to passive torque increases as n increases (Eq. 5), even while both quantities increase individuallẏ The increase in the ratio indicates an enhanced capability for active maneuvers and active stabilization, whereas the increase in FCT adds to passive stability. Thus, increasing wingbeat frequency enhances both maneuverability and stability. Hummingbirds provide an interesting example; males typically have greater wingbeat frequencies (21) and smaller body sizes as compared to females of the same species, potentially conferring a benefit in maneuverability and therefore an advantage in display flights (22) as well as greater stability when experiencing an external perturbation. These benefits are not without cost, because increasing wingbeat frequency increases the inertial and profile power requirements of flapping flight. Finally, the success of our FCT model in predicting yaw deceleration dynamics implies that passive damping may be important to flight control in flying animals across a wide range of body sizes. For example, if a steadily flapping animal experiences a brief perturbation in midstroke, by the time it is prepared to execute a corrective wingbeat, FCT will have eroded much of the effect of the perturbation, regardless of the wingbeat frequency employed by the animal. Thus, FCT provides open loop stability for some aspects of animal flight, reducing its neuromuscular and neurosensory requirements. These are not eliminated, because FCT results in asymmetric forces from symmetric flapping, implying that the animal's muscles must generate asymmetric forces and suggesting neural regulation to enforce symmetry. Furthermore, FCT does not address all the stability problems faced by flying animals. This study is limited to yaw dynamics in hovering or slow-speed flight; FCT is likely to be influential in fast forward flight, but no data are available to test such predictions. More important, a full description of body dynamics involves many factors beyond FCT and includes modes such as pitching and longitudinal dynamics known to be inherently unstable in open loop conditions (23, 24) and subject to active control (25, 26). Finally, yaw damping due to FCT is a feature of flapping flight that is not found in human-made fixed-wing or rotary-wing flyers and may lead to improvements in the stability and maneuverability of biomimetic micro-air vehicles. 11. S. P. Sane, J. Exp. Biol. 206, 4191 (2003). 12. J. R. Usherwood, C. P. Ellington, J. Exp. Biol. 205, 1565 Synonymous mutations do not alter the encoded protein, but they can influence gene expression. To investigate how, we engineered a synthetic library of 154 genes that varied randomly at synonymous sites, but all encoded the same green fluorescent protein (GFP). When expressed in Escherichia coli, GFP protein levels varied 250-fold across the library. GFP messenger RNA (mRNA) levels, mRNA degradation patterns, and bacterial growth rates also varied, but codon bias did not correlate with gene expression. Rather, the stability of mRNA folding near the ribosomal binding site explained more than half the variation in protein levels. In our analysis, mRNA folding and associated rates of translation initiation play a predominant role in shaping expression levels of individual genes, whereas codon bias influences global translation efficiency and cellular fitness. T he theory of codon bias posits that preferred codons correlate with the abundances of iso-accepting tRNAs (1, 2) and thereby increase translational efficiency (3) and accuracy (4). Recent experiments have revealed other effects of silent mutations (5-7). We synthesized a library of green fluorescent protein (GFP) genes that varied randomly in their codon usage, but encoded the same amino acid sequence (8). By placing these constructs in identical regulatory contexts and measuring their expression, we isolated the effects of synonymous variation on gene expression. The GFP gene consists of 240 codons. For 226 of these codons, we introduced random silent mutations in the third base position, while keeping the first and second positions constant We expressed the GFP genes in E. coli using a T7-promoter vector, and we quantified expression by spectrofluorometry. Fluorescence levels varied 250-fold across the library, and they were highly reproducible for each GFP construct (Spearman r = 0.98 between biological replicates)

Molecular, microbiological and clinical characterization of Clostridium difficile isolates from tertiary care hospitals in Colombia

In Colombia, the epidemiology and circulating genotypes of Clostridium difficile have not yet been described. Therefore, we molecularly characterized clinical isolates of C.difficile from patients with suspicion of C.difficile infection (CDI) in three tertiary care hospitals. C.difficile was isolated from stool samples by culture, the presence of A/B toxins were detected by enzyme immunoassay, cytotoxicity was tested by cell culture and the antimicrobial susceptibility determined. After DNA extraction, tcdA, tcdB and binary toxin (CDTa/CDTb) genes were detected by PCR, and PCR-ribotyping performed. From a total of 913 stool samples collected during 2013–2014, 775 were included in the study. The frequency of A/B toxins-positive samples was 9.7% (75/775). A total of 143 isolates of C.difficile were recovered from culture, 110 (76.9%) produced cytotoxic effect in cell culture, 100 (69.9%) were tcdA+/tcdB+, 11 (7.7%) tcdA-/tcdB+, 32 (22.4%) tcdA-/tcdB- and 25 (17.5%) CDTa+/CDTb+. From 37 ribotypes identified, ribotypes 591 (20%), 106 (9%) and 002 (7.9%) were the most prevalent; only one isolate corresponded to ribotype 027, four to ribotype 078 and four were new ribotypes (794,795, 804,805). All isolates were susceptible to vancomycin and metronidazole, while 85% and 7.7% were resistant to clindamycin and moxifloxacin, respectively. By multivariate analysis, significant risk factors associated to CDI were, staying in orthopedic service, exposure to third-generation cephalosporins and staying in an ICU before CDI symptoms; moreover, steroids showed to be a protector factor. These results revealed new C. difficile ribotypes and a high diversity profile circulating in Colombia different from those reported in America and European countries