Insoluble glycogen, a metabolizable internal adsorbent, decreases the lethality of endotoxin shock in rats

Insoluble glycogen is an enzymatically modified form of naturally occurring soluble glycogen with a great adsorbing capacity. It can be metabolized by phagocytes to glucose. In this study we used insoluble glycogen intravenously in the experimental endotoxin shock of rats. Wistar male rats were sensitized to endotoxin by Pb acetate. The survival of rats were compared in groups of animals endotoxin shock treated and non-treated with insoluble glycogen. Furthermore, we have determined in vitro the binding capacity of insoluble glycogen for endotoxin, tumour necrosis factor alpha, interleukin-1 and secretable phospholipase A2. Use of 10 mg/kg dose of insoluble glycogen could completely prevent the lethality of shock induced by LD50 quantity of endotoxin in rats. All animals treated survived. Insoluble glycogen is a form of ‘metabolizable internal adsorbents’. It can potentially be used for treatment of septic shock

A Chemocentric Approach to the Identification of Cancer Targets

A novel chemocentric approach to identifying cancer-relevant targets is introduced. Starting with a large chemical collection, the strategy uses the list of small molecule hits arising from a differential cytotoxicity screening on tumor HCT116 and normal MRC-5 cell lines to identify proteins associated with cancer emerging from a differential virtual target profiling of the most selective compounds detected in both cell lines. It is shown that this smart combination of differential in vitro and in silico screenings (DIVISS) is capable of detecting a list of proteins that are already well accepted cancer drug targets, while complementing it with additional proteins that, targeted selectively or in combination with others, could lead to synergistic benefits for cancer therapeutics. The complete list of 115 proteins identified as being hit uniquely by compounds showing selective antiproliferative effects for tumor cell lines is provided

Neue Angaben zur Spätneolithischen Siedlungsstruktur in Südosttransdanubien

The paper presents the recent survey results achieved at four Late Neolithic sites with rondels in Baranya County, Hungary (Villánykövesd-Jakabfalusi út mente, Zengővárkony-Igaz-dűlő, Szemely-Hegyes, Belvárdgyula-Szarkahegy). The combined use of non-destructive archaeological prospecting methods (aerial survey, field survey, magnetometer survey) and occassional trial trenching revealed new pieces of information that change our view of the Late Neolithic settlement structure in the region. Such observations are: the fact that rondels are present at virtually every major site in South-East Baranya the presence of special structural elements known only in South Transdanubia, like U-shaped blocking of entrances, special gate structures the large size of rondels when compared to other areas of longer history of research, like Lower Austria and Slovakia. These phenomena suggest the existence of a local „architectural style“ and may be indicative of a local group within the Lengyel Culture sphere