The role of salt bridges, charge density, and subunit flexibility in determining disassembly routes of protein complexes

Mass spectrometry can be used to characterize multiprotein complexes, defining their subunit stoichiometry and composition following solution disruption and collision-induced dissociation (CID). While CID of protein complexes in the gas phase typically results in the dissociation of unfolded subunits, a second atypical route is possible wherein compact subunits or subcomplexes are ejected without unfolding. Because tertiary structure and subunit interactions may be retained, this is the preferred route for structural investigations. How can we influence which pathway is adopted? By studying properties of a series of homomeric and heteromeric protein complexes and varying their overall charge in solution, we found that low subunit flexibility, higher charge densities, fewer salt bridges, and smaller interfaces are likely to be involved in promoting dissociation routes without unfolding. Manipulating the charge on a protein complex therefore enables us to direct dissociation through structurally informative pathways that mimic those followed in solution

Recommendations for reporting ion mobility Mass Spectrometry measurements

Here we present a guide to ion mobility mass spectrometry experiments, which covers both linear and nonlinear methods: what is measured, how the measurements are done, and how to report the results, including the uncertainties of mobility and collision cross section values. The guide aims to clarify some possibly confusing concepts, and the reporting recommendations should help researchers, authors and reviewers to contribute comprehensive reports, so that the ion mobility data can be reused more confidently. Starting from the concept of the definition of the measurand, we emphasize that (i) mobility values (K0) depend intrinsically on ion structure, the nature of the bath gas, temperature, and E/N; (ii) ion mobility does not measure molecular surfaces directly, but collision cross section (CCS) values are derived from mobility values using a physical model; (iii) methods relying on calibration are empirical (and thus may provide method‐dependent results) only if the gas nature, temperature or E/N cannot match those of the primary method. Our analysis highlights the urgency of a community effort toward establishing primary standards and reference materials for ion mobility, and provides recommendations to do so. © 2019 The Authors. Mass Spectrometry Reviews Published by Wiley Periodicals, Inc

Reentrant superconductivity in superconductor/ferromagnetic-alloy bilayers

We studied the Fulde-Ferrell-Larkin-Ovchinnikov (FFLO) like state establishing due to the proximity effect in superconducting Nb/Cu41Ni59 bilayers. Using a special wedge-type deposition technique, series of 20-35 samples could be fabricated by magnetron sputtering during one run. The layer thickness of only a few nanometers, the composition of the alloy, and the quality of interfaces were controlled by Rutherford backscattering spectrometry, high resolution transmission electron microscopy, and Auger spectroscopy. The magnetic properties of the ferromagnetic alloy layer were characterized with superconducting quantum interference device (SQUID) magnetometry. These studies yield precise information about the thickness, and demonstrate the homogeneity of the alloy composition and magnetic properties along the sample series. The dependencies of the critical temperature on the Nb and Cu41Ni59 layer thickness, Tc(dS) and Tc(dF), were investigated for constant thickness dF of the magnetic alloy layer and dS of the superconducting layer, respectively. All types of non-monotonic behaviors of Tc versus dF predicted by the theory could be realized experimentally: from reentrant superconducting behavior with a broad extinction region to a slight suppression of superconductivity with a shallow minimum. Even a double extinction of superconductivity was observed, giving evidence for the multiple reentrant behavior predicted by theory. All critical temperature curves were fitted with suitable sets of parameters. Then, Tc(dF) diagrams of a hypothetical F/S/F spin-switch core structure were calculated using these parameters. Finally, superconducting spin-switch fabrication issues are discussed in detail in view of the achieved results.Comment: 34 pages, 9 figure

Unification of bulk and interface electroresistive switching in oxide systems

We demonstrate that the physical mechanism behind electroresistive switching in oxide Schottky systems is electroformation, as in insulating oxides. Negative resistance shown by the hysteretic current-voltage curves proves that impact ionization is at the origin of the switching. Analyses of the capacitance-voltage and conductance-voltage curves through a simple model show that an atomic rearrangement is involved in the process. Switching in these systems is a bulk effect, not strictly confined at the interface but at the charge space region.Comment: 4 pages, 3 figures, accepted in PR

Structural health monitoring of offshore wind turbines: A review through the Statistical Pattern Recognition Paradigm

Offshore Wind has become the most profitable renewable energy source due to the remarkable development it has experienced in Europe over the last decade. In this paper, a review of Structural Health Monitoring Systems (SHMS) for offshore wind turbines (OWT) has been carried out considering the topic as a Statistical Pattern Recognition problem. Therefore, each one of the stages of this paradigm has been reviewed focusing on OWT application. These stages are: Operational Evaluation; Data Acquisition, Normalization and Cleansing; Feature Extraction and Information Condensation; and Statistical Model Development. It is expected that optimizing each stage, SHMS can contribute to the development of efficient Condition-Based Maintenance Strategies. Optimizing this strategy will help reduce labor costs of OWTs׳ inspection, avoid unnecessary maintenance, identify design weaknesses before failure, improve the availability of power production while preventing wind turbines׳ overloading, therefore, maximizing the investments׳ return. In the forthcoming years, a growing interest in SHM technologies for OWT is expected, enhancing the potential of offshore wind farm deployments further offshore. Increasing efficiency in operational management will contribute towards achieving UK׳s 2020 and 2050 targets, through ultimately reducing the Levelised Cost of Energy (LCOE)

MU670 Chamber Music Recital, February 27, 1989

This is the concert program of the MU670 Chamber Music Recital on Monday, February 27, 1989 at 6:30 p.m., at the Marshall Room, 855 Commonwealth Avenue. Works performed were Quintet by Carl Nielsen, Trio, Op. 1, No. 3 by Ludwig van Beethoven, Quintet in C major, Op. 163 by Franz Schubert, Serenade No. 10 by Vincent Persichetti, Entreacte by Jacques Ibert, Quintet, Op. 77, G major by Antonín Dvořák, Nostalgia d'Espana by Gordon Lewin, and Allegro from Trio in C major, Op. 87 by Johannes Brahms. Digitization for Boston University Concert Programs was supported by the Boston University Humanities Library Endowed Fund