Sutureless Aortic Valve Replacement – the First Experience in Trakya University

DergiPark: 378995tmsjIntroduction: We present the first experience of sutureless aortic bioprosthesis valve replacement in Trakya University Cardiovascular Surgery Clinic. Case report:A 71 year-old female patient with shortness of breath admitted to the cardiology clinic. In physical examination, there was a 3/6 systolic murmur on aortic area spreading to the neck. In the echocardiography, severe aortic stenosis and mild aortic insufficiency was found. In coronary angiography, 90% stenosis was found on proximal portion of left anterior descending coronary artery. Aortic valve replacement and coronary artery by-pass operation was planned. Results: The first sutureless aortic valve replacement in Trakya University Hospital was performed successfully and the patient was discharged from the hospital without any complication

Evaluating Combined Effect of Naringin and Salicylic Acid on Colon Cancer Cell Culture

DergiPark: 379061tmsjAims: Colorectal cancer is the 3rd most common cancer in the world. It affects more than a million people and causes the death of half million people annually. Flavonoids are natural products belonging to plantae and some fungal organisms that recently have started to be popular for cancer research for its strong antioxidant, anticarcinogen and anti-tumor properties. Naringin is a special chemical compound of flavonoid groups in grapefruit and useful for its antioxidant and immunostimulatory properties. Salicylic acid is a stress-specific hormone that also has an anti-tumor effects on colorectal cancer. In this study, it is aimed to evaluate the effect of naringin, salicylic acid and their combination on colon cancer cells via gene expression profiles of apoptosis genes and anti-proliferative properties. Methods: HT29 colon cell culture was incubated in 37 C and 5% CO2. Salicylic acid, naringin and their combinations were applied seperetaly on 80% confluent cells in 11 different doses starting with 800 µM and going half of the previous. MTT survival test was performed at 24th and 48th hours after application. To see the effect on apoptosis and antioxidant pathway; apoptotic protease activating factor, B-cell lymphoma 2, B-cell lymphoma 2 associated X, B-cell lymphoma 2 - XL, Cytochrome C, Cellular inhibitor of apoptosis protein 1, Cellular inhibitor of apoptosis protein 2, Glyceraldehyde-3-phosphate dehydrogenas, Caspase 3, Livin, Survivin, p21, p27, p53 and X-linked inhibitor of apoptosis, Catalase, Glutathione peroxidase, Superoxide dismutase 1 and Superoxide dismutase 2 gene expressions were assayed on 24th and 48th hours by using real time PCR.Results: Single and combined application of naringin and salicylic acid decreased cell proliferation at both 24th and 48th hours. Results in 48th hours were more obvious. None of the applications caused an increase in number of cells in any applied dose. In the real time PCR analysis, the expressions of apoptosis inhibitor genes that play a crucial role in antioxidant pathway were increased. The increase was more distinct in the combination of naringin and salicylic acid.Conclusion: In this study, it is found that both salicylic acid and naringin cause a decrease in the number of colon cell culture. As for their combination it also worked well. The increase in apoptotic gene expression was exclusive. It can be said that naringin, salicylic acid and especially their combination can be a promising treatment as a supported option for colon cancer patients in the futur

What Is Your Diagnosis?

DergiPark: 379011tmsj[Abstract Not Available

Evaluating the Prevalence of Depression, Sleep Quality and Social Phobia Among Trakya University Hospital Residents

DergiPark: 379051tmsjAims: The purpose of this study is to determine and evaluate the depression, sleep quality and social anxiety levels among medical residents at Trakya University Hospital.Methods: This questionnaire study was conducted among 105 voluntary medical residents at Trakya University Hospital. Beck Depression Inventory, Pitssburgh Sleep Quality Index and Liebowitz Social Anxiety Scale was used to assess depression, sleep quality and social phobia. As for statistical analyses, chi-square, Kruskal Wallis and Mann Whitney Tests were used for analyzing categorical and continuous data.Results: It was found out that 39% of them had depressive symptoms ranging from “mild depressive” to “very severe depressive”, when 14.3% had poor sleep quality and 87.6% had social anxiety symptoms ranging from “moderate social anxiety” to “very severe social anxiety”. Beck Depression Score for surgical residents was significantly higher than basic sciences residents, when female residents had a significantly higher score for social anxiety in comparison with male residents.Conclusion: The prevalence of depression, poor sleep quality and social anxiety among residents was found to be higher than expected which might be due to stress, heavy workload and lifestyle of physician

Antinuclear antibody testing in a Turkish pediatrics clinic: is it always necessary?

Introduction: the term anti-nuclear antibody (ANA) is used to define a large group of autoantibodies which specifically bind to nuclear elements. Although healthy individuals may also have ANA positivity, the measurement of ANA is generally used in the diagnosis of autoimmune disorders. However, various studies have shown that ANA testing may be overused, especially in pediatrics clinics. Our aim was to investigate the reasons for antinuclear antibody (ANA) testing in the general pediatrics and pediatric rheumatology clinics of our hospital and to determine whether ANA testing was ordered appropriately by evaluating chief complaints and the ultimate diagnoses of these cases. Methods: the medical records of pediatric patients in whom ANA testing was performed between January 2014 and June 2016 were retrospectively evaluated. Subjects were grouped according to the indication for ANA testing and ANA titers. Results: ANA tests were ordered in a total of 409 patients during the study period, with 113 positive ANA results. The ANA test was ordered mostly due to joint pain (50% of the study population). There was an increased likelihood of autoimmune rheumatic diseases (ARDs) with higher ANA titer. The positive predictive value of an ANA test was 16% for any connective tissue disease and 13% for lupus in the pediatric setting. Conclusion: in the current study, more than one-fourth of the subjects were found to have ANA positivity, while only 15% were ultimately diagnosed with ARDs. Our findings underline the importance of an increased awareness of correct indications for ANA testing

PİYASADAN TEMİN EDİLEN BAZI YAĞLARIN ANTİOKSİDAN AKTİVİTESİNİN ÇEŞİTLİ YÖNTEMLERLE DEĞERLENDİRİLMESİ

Sabit yağlar ve uçucu yağlar, yüzyıllardır insanlar tarafından, hastalıkların tedavi edilmesinde, hastalıklardan korunmak amacıyla ve yaşam kalitesini artırmak için kullanılmaktadır. Ayrıca çoğu bitkisel kökenli olan, özellikle bitkilerin yağlı tohumlarından elde edilen yağlar, aromaterapide “taşıyıcı yağ” olarak kullanılmaktadır. Tedavide kullanılan bu yağların yapısı, kalitesi, elde ediliş şekli ve tedaviye uygun olarak seçilmesi, yağın tedaviye olumlu katkı vermesini sağlayan en önemli etmendir. Özellikle yaşlanma karşıtı ürünlerde tercih edilen yağlar antioksidan özellikleri ile ön plana çıkmakta ve bu yönüyle tedaviye destek olmaktadırlar. Çalışmada da bu amaca yönelik olarak piyasada bulunan bazı yağların antioksidan aktivitesi karşılaştırılmıştır. Yöntem olarak 1,1-difenil-2-pikrilhidrazil (DPPH•) radikal süpürücü etki, 2,2’- azino-bis (3-etilbenzotiazolin-6- sulfonik asit) (ABTS+•) radikal süpürücü etki ve askorbat–Fe (III)-katalizli fosfolipit peroksidasyonunu engelleyici etki tayini yöntemleri kullanılmıştır. Deneysel çalışmalarda kullanılan buğday rüşeymi (Triticum sativum), keten tohumu (Linum usitatissimum), nar çekirdeği (Punica granatum), kişniş (Coriandrium sativum), çörek otu (Nigella sativa), sakız kabağı tohumu yağları (Cucurbita sp.) ve zerdeçal ekstresi (Curcuma longa) piyasadan temin edilmiştir. Yapılan çalışmalar sonucunda zerdeçal ekstresi ve buğday rüşeym yağı en aktif yağlar olarak belirlenmişlerdir

Physicochemistry, microbiology, fatty acids composition and volatile profile of traditional Söğle tulum (goat’s skin bag) cheese

Abstract Söğle cheese is a type of traditional tulum (goat’s skin bag) cheese produced from goat’s milk or a mixture of goat’s and sheep’s milk in Küçük Söğle and Büyük Söğle villages, Antalya, Turkey. This is the first study that aimed to investigate the physicochemistry, microbiology, fatty acids composition and volatile profile of traditional Söğle cheeses ripened in goat’s skins for three months in wells. The pH and titratable acidity values, total solids, fat, protein and salt contents of the Söğle cheese samples ranged from 4.9 to 5.5, from 1.4 to 2.6%, from 46.5 to 55.0%, from 2.0 to 4.5%, from 34.9 and 42.2% and from 4.1 to 8.2%, respectively. The highest proportion of fatty acids in all cheese samples was palmitic acid (C16:0). The lactobacillus, lactococcus, total aerobic mesophilic bacteria and yeast and mold counts in the cheese samples were found to be between 7.1 and 8.5 log cfu/g, 7.8 and 8.7 log cfu/g, 7.6 and 8.5 log cfu/g and 1.0 and 4.8 log cfu/g, respectively. Fifty-three volatile components were identified in the cheese samples including 13 esters, 7 ketones, 5 acids, 2 aldehydes, 5 alcohols, 12 terpenes, and 9 miscellaneous compounds. Ketones and terpenes were the predominant volatile compounds