Speech-to-Speech synchronization protocol to classify human participants as high or low auditory-motor synchronizers

The ability to synchronize a motor action to a rhythmic auditory stimulus is often considered an innate human skill. However, some individuals lack the ability to synchronize speech to a perceived syllabic rate. Here, we describe a simple and fast protocol to classify a single native English speaker as being or not being a speech synchronizer. This protocol consists of four parts: the pretest instructions and volume adjustment, the training procedure, the execution of the main task, and data analysis

Programmed cell senescence during mammalian embryonic development

Cellular senescence disables proliferation in damaged cells, and it is relevant for cancer and aging. Here, we show that senescence occurs during mammalian embryonic development at multiple locations, including the mesonephros and the endolymphatic sac of the inner ear, which we have analyzed in detail. Mechanistically, senescence in both structures is strictly dependent on p21, but independent of DNA damage, p53, or other cell-cycle inhibitors, and it is regulated by the TGF-beta/SMAD and PI3K/FOXO pathways. Developmentally programmed senescence is followed by macrophage infiltration, clearance of senescent cells, and tissue remodeling. Loss of senescence due to the absence of p21 is partially compensated by apoptosis but still results in detectable developmental abnormalities. Importantly, the mesonephros and endolymphatic sac of human embryos also show evidence of senescence. We conclude that the role of developmentally programmed senescence is to promote tissue remodeling and propose that this is the evolutionary origin of damage-induced senescence

Parámetros hematológicos en polluelos de psitácidos en cautiverio de origen silvestre

El objetivo de este estudio fue describir los parámetros hematológicos en 69 polluelos en cautiverio de origen silvestre, clínicamente sanos, menores de tres meses de edad, en cuatro especies de psitácidos: Pionus senilis (n = 18), Amazona albifrons (n = 15), Amazona autumnalis (n = 25) y Amazona oratrix (n = 11), procedentes de la región central de México. Para determinar dichos parámetros, de cada ejemplar se obtuvieron 500 μl de sangre por punción de la vena braquial. En las cuatro especies citadas, el porcentaje del hematocrito fue de 56,4 ± 3,36; 51 ± 6,52; 47,6 ± 6,47 y 53,8 ± 9,26; el VCM en fl fue de 184,6 ± 13,4; 142,0 ± 25,4; 106,3 ± 11,7 y 162,3 ± 71,9; la cuenta eritrocítica fue de 4,7 ± 2,1 x106/μl; 6,1 ± 8,2 x106/μl; 6,9 ± 1,2 x106/μl y 5,4 ± 1,1 x106/μl; y la cuenta leucocitaria de 3,06 ± 0,15 x103/μl; 3,62 ± 0,24 x103/μl; 4,48 ± 0,36 x103/μl y 3,56 ± 0,71 x103/μl respectivamente para P. senilis, A. albifrons, A. autumnalis y A. oratrix. Se constató tendencia a la heterofilia en A. albifrons y A. oratrix, lo cual puede ser de gran utilidad para un diagnóstico cada vez más preciso en la clínica de aves en cautiverio

The relative effects of upwelling and river flow on the phytoplankton diversity patterns in the ria of A Coruña (NW Spain)

Phytoplankton species assemblages in estuaries are connected to those in rivers and marine environments by local hydrodynamics leading to a continuous flow of taxa. This study revealed differential effects of upwelling and river flow on phytoplankton communities observed in 2011 along a salinity gradient from a river reservoir connected to the sea through a ria-marine bay system in A Coruña (NW Spain, 43° 16-21’ N, 8° 16-22’ W). With 130 phytoplankton taxa identified, the assemblages were dominated in general by diatoms, particularly abundant in the bay and in the estuary, but also by chlorophycea and cyanobacteria in the reservoir. Considering the entire seasonal cycle, the local assemblages were mainly characterized by changes in cryptophytes and diatoms, small dinoflagellates and some freshwater chlorophycea. Salinity, nitrate, and organic matter variables, were the main environmental factors related to the changes in the phytoplankton communities through the system, while phosphate and nitrite were also important for local communities in the estuary and the bay, respectively. The corresponding local phytoplankton assemblages showed moderate levels of connectivity. The estuarine community shared a variable number of taxa with the adjacent zones, depending on the relative strength of upwelling (major influence from the bay) and river flow (major influence of the reservoir) but had on average 35% of unique taxa. Consequently, local and zonal diversity patterns varied seasonally and were not simply related to the salinity gradient driven by the river flow.ANILE (CTM2009-08396 and CTM2010-08804-E), FIOME (CTM2011-28792-C02-01-MAR), and MEFIO (CTM2011-28792-C02-02-MAR) of the Plan Nacional de I+D+i (Spain), and RADIALES of the Instituto Español de Oceanografía (IEO, Spain).Versión del editor2,01

Nucleofection induces non-specific changes in the metabolic activity of transfected cells

Transfection has become an everyday technique widely used for functional studies in living cells. The choice of the particular transfection method is usually determined by its efficiency and toxicity, and possible functional consequences specific to the method used are normally overlooked. We describe here that nucleofection, a method increasingly used because of its convenience and high efficiency, increases the metabolic rate of some cancer cells, which can be misleading when used as a measure of proliferation. Moreover, nucleofection can alter the subcellular expression pattern of the transfected protein. These undesired effects are independent of the transfected nucleic acid, but depend on the particular cell line used. Therefore, the interpretation of functional data using this technology requires further controls and caution

Situación fitosanitaria de los principales sistemas de producción en el Valle del Cauca durante el año de 1997.

El Instituto Colombiano Agropocuario ICA, as Is ontidad responsablo do Pa sanidad agrlcola y pecuarla del pals a través do Is coordlnaclon, I. planoacion, dirección y ojecucion do programas do protecclon y control do todas lag situacionos do riosgo pare Is productividad agropocuaria uttlizando raclonales criterios do compotltividad, sostoniblildad y oquidad con el proposito do participar efoctivamonto on all dosarrollo economico y social del pals. En oste sontido, ontre los diforontes proyectos quo so ojecutan an al Dopartamonto del Valle del Cauca as oncuontre: Diagnostico, vigilancia y alerts fitosanitarla an los principales sistemas do producclon on 01 Valle del Cauca", quo adelantan los técnicos do Sanidad Vegetal on oats Seccional. Como resultado prollmlnar do [as acciones do Vigitancla Fltosanitarta este documento pretends ponor en conoclmlonto do los usuarlos intermediaries del departamento (ontidades del orden municipal y dopartamontal) Is situaciôn fitosanitaria do los principales sistemas do prcducclón (cane do azUcar, café, algodOn, maiz, sorgo, soya, plétano, banano y tomato) on ol Valle del Cauca durante 1997. Las especies restantos so Inoorporarén al sistema do Informaclôn do Vlgilancla fitosanitaila on Is rnedlda an quo so Involucren como fuentes do informaclón todas In entidados del sector agropecuarlo ubicadal on los municiplos del dopartamento

Hospital Virtual: Sistema de información clínica y telecuidado de pacientes VIH/SIDA basado en tecnologías Web 2.0

Este artículo describe el desarrollo de un nuevo sistema de información clínica y telecuidado de pacientes VIH/SIDA que se encuentra implantado en rutina clínica dentro del Servicio de Enfermedades Infecciosas del Hospital Clínic de Barcelona. El proyecto surge ante la necesidad de unificar el sistema de información departamental del servicio y un sistema de telemedicina instalado en el hospital en 2004. Para ello se han diseñado e implementado nuevas bases de datos y dos sistemas específicos para uso de profesionales y pacientes. Además, se ha realizado una integración con el Sistema de Información del Hospital (HIS) permitiendo el acceso a todos los datos requeridos de los pacientes desde una única aplicación. Este nuevo sistema está al servicio de más de 70 profesionales sanitarios que realizan una media de 150 consultas al día disponiendo de información clínica de más de 8000 pacientes

NLRP3 inflammasome suppression improves longevity and prevents cardiac aging in male mice

While NLRP3‐inflammasome has been implicated in cardiovascular diseases, its role in physiological cardiac aging is largely unknown. During aging, many alterations occur in the organism, which are associated with progressive impairment of metabolic pathways related to insulin resistance, autophagy dysfunction, and inflammation. Here, we investigated the molecular mechanisms through which NLRP3 inhibition may attenuate cardiac aging. Ablation of NLRP3‐inflammasome protected mice from age‐related increased insulin sensitivity, reduced IGF‐1 and leptin/adiponectin ratio levels, and reduced cardiac damage with protection of the prolongation of the agedependent PR interval, which is associated with atrial fibrillation by cardiovascular aging and reduced telomere shortening. Furthermore, old NLRP3 KO mice showed an inhibition of the PI3K/AKT/mTOR pathway and autophagy improvement, compared with old wild mice and preserved Nampt‐mediated NAD+ levels with increased SIRT1 protein expression. These findings suggest that suppression of NLRP3 prevented many age‐associated changes in the heart, preserved cardiac function of aged mice and increased lifespan.Andalusian regional government; Consejería de Salud de la Junta de Andalucia, Grant/ Award Number: PI‐0036‐2014; Ministerio de economía y competitividad, Grant/Award Number: SAF2017‐84494‐C2‐1‐

CMS DAQ Event Builder Based on Gigabit Ethernet

The CMS Data Acquisition system is designed to build and filter events originating from approximately 500 data sources from the detector at a maximum Level 1 trigger rate of 100 kHz and with an aggregate throughput of 100 GByte/s. For this purpose different architectures and switch technologies have been evaluated. Events will be built in two stages: the first stage, the FED Builder, will be based on Myrinet technology and will pre-assemble groups of about 8 data sources. The next stage, the Readout Builder, will perform the building of full events. The requirement of one Readout Builder is to build events at 12.5 kHz with average size of 16 kBytes from 64 sources. In this paper we present the prospects of a Readout Builder based on TCP/IP over Gigabit Ethernet. Various Readout Builder architectures that we are considering are discussed. The results of throughput measurements and scaling performance are outlined as well as the preliminary estimates of the final performance. All these studies have been carried out at our test-bed farms that are made up of a total of 130 dual Xeon PCs interconnected with Myrinet and Gigabit Ethernet networking and switching technologies