23 research outputs found
Gas chromatography vs. quantum cascade laser-based N<sub>2</sub>O flux measurements using a novel chamber design
Recent advances in laser spectrometry offer new opportunities to
investigate the soil–atmosphere exchange of nitrous oxide. During two field
campaigns conducted at a grassland site and a willow field, we tested the
performance of a quantum cascade laser (QCL) connected to a newly developed
automated chamber system against a conventional gas chromatography (GC)
approach using the same chambers plus an automated gas sampling unit with
septum capped vials and subsequent laboratory GC analysis. Through its high
precision and time resolution, data of the QCL system were used for
quantifying the commonly observed nonlinearity in concentration changes
during chamber deployment, making the calculation of exchange fluxes more
accurate by the application of exponential models. As expected, the curvature
values in the concentration increase was higher during long (60 min) chamber
closure times and under high-flux conditions
(FN2O > 150 µg N m−2 h−1)
than those values that were found when chambers were closed for only 10 min and/or
when fluxes were in a typical range of 2 to
50 µg N m−2 h−1. Extremely low standard errors of
fluxes, i.e., from  ∼  0.2 to 1.7 % of the flux value, were observed
regardless of linear or exponential flux calculation when using QCL data.
Thus, we recommend reducing chamber closure times to a maximum of 10 min
when a fast-response analyzer is available and this type of chamber system is
used to keep soil disturbance low and conditions around the chamber plot as
natural as possible. Further, applying linear regression to a 3 min data
window with rejecting the first 2 min after closure and a sampling time
of every 5 s proved to be sufficient for robust flux determination while ensuring
that standard errors of N2O fluxes were still on a relatively low level.
Despite low signal-to-noise ratios, GC was still found to be a useful method
to determine the mean the soil–atmosphere exchange of N2O on longer timescales
during specific campaigns. Intriguingly, the consistency between GC and
QCL-based campaign averages was better under low than under high N2O
efflux conditions, although single flux values were highly scattered during
the low efflux campaign. Furthermore, the QCL technology provides a useful
tool to accurately investigate the highly debated topic of diurnal courses
of N2O fluxes and its controlling factors. Our new chamber design
protects the measurement spot from unintended shading and minimizes
disturbance of throughfall, thereby complying with high quality requirements
of long-term observation studies and research infrastructures
A higher-moment CAPM of Korean stock returns
The traditional Capital Asset Pricing Model (CAPM) developed by Sharpe, Lintner and Mossin is based on the strong assumption of normally distributed returns among other restrictions. However, especially in emerging stock markets, returns often deviate from normality, even though the series are of lower frequency. This paper extends upon the traditional framework of the expected equilibrium return of Korean stocks by incorporating higher order moments in order to explain their risk-return characteristics. Empirical evidence shows that a higher-moment CAPM increases the explanatory power of the return generating process. Particularly, in up-market phases, where the return on the market portfolio exceeds the risk-free interest rate, expected return, covariance, co-skewness and co-kurtosis are related.co-skewness; co-kurtosis; higher-moment CAPM; return generating process; emerging markets; Korea; market conditions; risk premia; stock returns; capital asset pricing model; stock markets; emerging markets.
Standardisation of chamber technique for CO2, N2O and CH4 fluxes measurements from terrestrial ecosystems
Chamber measurements of trace gas fluxes between the land surface and the atmosphere have been conducted for almost a century. Different chamber techniques, including static and dynamic, have been used with varying degrees of success in estimating greenhouse gases (CO2, CH4, N2O) fluxes. However, all of these have certain disadvantages which have either prevented them from providing an adequate estimate of greenhouse gas exchange or restricted them to be used under limited conditions. Generally, chamber methods are relatively low in cost and simple to operate. In combination with the appropriate sample allocations, chamber methods are adaptable for a wide variety of studies from local to global spatial scales, and they are particularly well suited for in situ and laboratory-based studies. Consequently, chamber measurements will play an important role in the portfolio of the Pan-European long-term research infrastructure Integrated Carbon Observation System. The respective working group of the Integrated Carbon Observation System Ecosystem Monitoring Station Assembly has decided to ascertain standards and quality checks for automated and manual chamber systems instead of defining one or several standard systems provided by commercial manufacturers in order to define minimum requirements for chamber measurements. The defined requirements and recommendations related to chamber measurements are described here.Peer reviewe
LIN28B enhanced tumorigenesis in an autochthonous KRAS(G12V)-driven lung carcinoma mouse model
LIN28B is a RNA-binding protein regulating predominantly let-7 microRNAs with essential functions in inflammation, wound healing, embryonic stem cells, and cancer. LIN28B expression is associated with tumor initiation, progression, resistance, and poor outcome in several solid cancers, including lung cancer. However, the functional role of LIN28B, especially in non-small cell lung adenocarcinomas, remains elusive. Here, we investigated the effects of LIN28B expression on lung tumorigenesis using LIN28B transgenic overexpression in an autochthonous KRAS(G12V)-driven mouse model. We found that LIN28B overexpression significantly increased the number of CD44+/CD326+ tumor cells, upregulated VEGF-A and miR-21 and promoted tumor angiogenesis and epithelial-to-mesenchymal transition (EMT) accompanied by enhanced AKT phosphorylation and nuclear translocation of c-MYC. Moreover, LIN28B accelerated tumor initiation and enhanced proliferation which led to a shortened overall survival. In addition, we analyzed lung adenocarcinomas of the Cancer Genome Atlas (TCGA) and found LIN28B expression in 24% of KRAS-mutated cases, which underscore the relevance of our model