Effect of pH and level of concentrate in the diet on the production of biohydrogenation intermediates in a dual-flow continuous culture

Milk fat depression in cows fed high-grain diets has been related to an increase in the concentration of trans-10 C-18:1 and trans-10, cis-12 conjugated linoleic acid (CLA) in milk. These fatty acids (FA) are produced as a result of the alteration in rumen biohydrogenation of dietary unsaturated FA. Because a reduction in ruminal pH is usually observed when high-concentrate diets are fed, the main cause that determines the alteration in the biohydrogenation pathways is not clear. The effect of pH (6.4 vs. 5.6) and dietary forage to concentrate ratios (F:C; 70:30 F:C vs. 30:70 F:C) on rumen microbial fermentation, effluent FA profile, and DNA concentration of bacteria involved in lipolysis and biohydrogenation processes were investigated in a continuous culture trial. The dual-flow continuous culture consisted of 2 periods of 8 d (5 d for adaptation and 3 d for sampling), with a 2 x 2 factorial arrangement of treatments. Samples from solid and liquid mixed effluents were taken for determination of total N, ammonia-N, and volatile fatty acid concentrations, and the remainder of the sample was lyophilized. Dry samples were analyzed for dry matter, ash, neutral and acid detergent fiber, FA, and purine contents. The pH 5.6 reduced organic matter and fiber digestibility, ammonia-N concentration and flow, and crude protein degradation, and increased nonammonia and dietary N flows. The pH 5.6 decreased the flow of C-18:0, trans-11 C-18:1 and cis-9, trans-11 CLA, and increased the flow of trans-10 C-18:1, C18:2n-6, C18:3n-3, trans-11, cis-15 C-18:2 and trans-10, cis-12 CLA in the 1 h after feeding effluent. The pH 5.6 reduced Anaerovibrio lipolytica (32.7 vs. 72.1 pg/10 ng of total DNA) and Butyrivibrio fibrisolvens vaccenic acid subgroup (588 vs. 1,394 pg/10 ng of total DNA) DNA concentrations. The high-concentrate diet increased organic matter and fiber digestibility, nonammonia and bacterial N flows, and reduced ammonia-N concentration and flow. The high-concentrate diet reduced trans-11 C-18:1 and trans-10 C-18:1, and increased C18:2n-6, C18:3n-3 and trans-10, cis-12 CLA proportions in the 1 h after feeding effluent. The increase observed in trans-10, cis-12 CLA proportion in the 1 h after feeding effluent due to the high-concentrate diet was smaller that that observed at pH 5.6. Results indicate that the pH is the main cause of the accumulation of trans-10 C-18:1 and trans-10, cis-12 CLA in the effluent, but the trans-10, cis-12 CLA proportion can be also affected by high levels of concentrate in the diet

Inelastic collisions in an exactly solvable two-mode Bose-Einstein Condensate

Inelastic collisions occur in Bose-Einstein condensates, in some cases, producing particle loss in the system. Nevertheless, these processes have not been studied in the case when particles do not escape the trap. We show that such inelastic processes are relevant in quantum properties of the system such as the evolution of the relative population, the self trapping effect and the probability distribution of particles. Moreover, including inelastic terms in the model of the two-mode condensate allows for an exact analytical solution. Using this solution, we show that collisions favor the generation of entanglement between the modes of the condensate as long as the collision rate does not exceed the natural frequency of the system

Noise-induced phase transitions: Effects of the noises' statistics and spectrum

The local, uncorrelated multiplicative noises driving a second-order, purely noise-induced, ordering phase transition (NIPT) were assumed to be Gaussian and white in the model of [Phys. Rev. Lett. \textbf{73}, 3395 (1994)]. The potential scientific and technological interest of this phenomenon calls for a study of the effects of the noises' statistics and spectrum. This task is facilitated if these noises are dynamically generated by means of stochastic differential equations (SDE) driven by white noises. One such case is that of Ornstein--Uhlenbeck noises which are stationary, with Gaussian pdf and a variance reduced by the self-correlation time (\tau), and whose effect on the NIPT phase diagram has been studied some time ago. Another such case is when the stationary pdf is a (colored) Tsallis' (q)--\emph{Gaussian} which, being a \emph{fat-tail} distribution for (q>1) and a \emph{compact-support} one for (q<1), allows for a controlled exploration of the effects of the departure from Gaussian statistics. As done before with stochastic resonance and other phenomena, we now exploit this tool to study--within a simple mean-field approximation and with an emphasis on the \emph{order parameter} and the ``\emph{susceptibility}''--the combined effect on NIPT of the noises' statistics and spectrum. Even for relatively small (\tau), it is shown that whereas fat-tail noise distributions ((q>1)) counteract the effect of self-correlation, compact-support ones ((q<1)) enhance it. Also, an interesting effect on the susceptibility is seen in the last case.Comment: 6 pages, 10 figures, uses aipproc.cls, aip-8s.clo and aipxfm.sty. To appear in AIP Conference Proceedings. Invited talk at MEDYFINOL'06 (XV Conference on Nonequilibrium Statistical Mechanics and Nonlinear Physics

Stochastic Resonance: influence of a f−κf^{-\kappa} noise spectrum

Here, in order to study \textit{stochastic resonance} (SR) in a double-well potential when the noise source has a spectral density of the form $f^{-\kappa}$ with varying $\kappa$, we have extended a procedure, introduced by Kaulakys et al (Phys. Rev. E \textbf{70}, 020101 (2004)). In order to have an analytical understanding of the results, we have obtained an effective Markovian approximation, that allows us to make a systematic study of the effect of such kind of noises on the SR phenomenon. The comparison of numerical and analytical results shows an excellent qualitative agreement indicating that the effective Markovian approximation is able to correctly describe the general trends.Comment: 11 pages, 6 figures, submitted to Euro.Phys.J.

Increased intestinal carbonate precipitate abundance in the sea bream (Sparus aurata L.) in response to ocean acidification

Marine fish contribute to the carbon cycle by producing mineralized intestinal precipitates generated as by-products of their osmoregulation. Here we aimed at characterizing the control of epithelial bicarbonate secretion and intestinal precipitate presence in the gilthead sea bream in response to predicted near future increases of environmental CO2. Our results demonstrate that hypercapnia (950 and 1800 μatm CO2) elicits higher intestine epithelial HCO3- secretion ex vivo and a subsequent parallel increase of intestinal precipitate presence in vivo when compared to present values (440 μatm CO2). Intestinal gene expression analysis in response to environmental hypercapnia revealed the up-regulation of transporters involved in the intestinal bicarbonate secretion cascade such as the basolateral sodium bicarbonate co-transporter slc4a4, and the apical anion transporters slc26a3 and slc26a6 of sea bream. In addition, other genes involved in intestinal ion uptake linked to water absorption such as the apical nkcc2 and aquaporin 1b expression, indicating that hypercapnia influences different levels of intestinal physiology. Taken together the current results are consistent with an intestinal physiological response leading to higher bicarbonate secretion in the intestine of the sea bream paralleled by increased luminal carbonate precipitate abundance and the main related transporters in response to ocean acidification.Agência financiadora Fundacao para a Ciencia e a Tecnologia (FCT) SFRH/BD/113363/2015 PTDC/MAR-BIO/3034/2014 Fundacao para a Ciencia e a Tecnologia (FCT) UID/Multi/04326/2019 Ministry of Science and Higher Education, Polandinfo:eu-repo/semantics/publishedVersio

ApuA, a multifunctional x-glucan-degrading enzyme of Streptococcus suis, mediates adhesion to porcine epithelium and mucus

We have identified apuA in Streptococcus suis, which encodes a bifunctional amylopullulanase with conserved -amylase and pullulanase substrate-binding domains and catalytic motifs. ApuA exhibited properties typical of a Gram-positive surface protein, with a putative signal sequence and LPKTGE cell-wall-anchoring motif. A recombinant protein containing the predicted N-terminal -amylase domain of ApuA was shown to have -(1,4) glycosidic activity. Additionally, an apuA mutant of S. suis lacked the pullulanase -(1,6) glycosidic activity detected in a cell-surface protein extract of wild-type S. suis. ApuA was required for normal growth in complex medium containing pullulan as the major carbon source, suggesting that this enzyme plays a role in nutrient acquisition in vivo via the degradation of glycogen and food-derived starch in the nasopharyngeal and oral cavities. ApuA was shown to promote adhesion to porcine epithelium and mucus in vitro, highlighting a link between carbohydrate utilization and the ability of S. suis to colonize and infect the host

Potential of short wavelength laser ablation of organic materials

Although the literature contains several articles on UV laser ablation of synthetic polymers [1] and human tissue for surgical applications, to our knowledge there is no published record on organic geochemical applications for UV laser pyrolysis–gas chromatography–mass spectrometry (LA-GC-MS). In this study we have demonstrated the use of a 213 nm UV laser beam for ablating kerogens and organic rich rocks to liberate and analyse hydrocarbon signatures and compared the results against IR laser pyrolysis and traditional Py-GC-MS. It is possible to equate laser wavelength to electron volts where 1064 nm (IR) = 1.2 eV and 213 nm (UV) = 5.8 eV. Most chemical bonds have an energy between 2-4 eV and C-C bonds are ~3.6 eV. Organic materials can absorb radiation from a UV laser and chemical bonds can be cleaved cleanly by complex photochemical pathways by a single photon [2]. Ablation occurs with almost no heating of the sample and hence the term laser ablation instead of pyrolysis. Visible or IR lasers have insufficient energy to break bonds with a single photon this results in the heating of sample by the absobtion of energy into the vibrational modes of the molecule which can then result in pyrolysis. A solvent-extracted kerogen consisting mainly of higher plant material (Brownie Butte, Montanna, ~ 70 Ma) was used for initial experiments. A number of other samples have also been analysed. Laser ablation work was performed off-line in a static helium cell followed by solvent extraction of the laser cell. Separate analysis of the same samples using a more traditional flash pyrolysis approach was performed with a CDS pyroprobe and IR laser pyrolysis [3] for comparative purposes. As can be seen in Fig 1 UV laser ablation is able to liberate relatively high molecular weight fragments with no alkenes or other pyrolysis artefacts detected. SEM images of ablation pits indicate there is no obvious thermal alteration of the sample. The results of the pyrolysis techniques (on-line and IR laser pyrolysis) are similar and display a number of artefacts related to the pyrolysis process. Laser ablation of a number of samples has also shown that the distributions of biomarkers are comparable with the solvent extracts. Product yields although not quantified appear to be much higher than traditional pyrolysis technique

Is UV laser ablation a suitable tool for geochemical analysis of organic rich source materials?

Abstract not available

Impact of a Culturally-Relevant Support Program and Latinx Adolescents\u27 Help Seeking Services: An Academic-Latinx Community Service Partnership To Enhance Culturally Responsive Services for Latinx Immigrant Youth.

Latinxs, in general, report logistical barriers that contribute to utilizing fewer healthcare services and/or not knowing how to navigate the healthcare system. In addition to underutilizing healthcare services, Latinx immigrant youth also report higher levels of negative attitudes towards mental health. However, little is still known regarding factors that impact these attitudes with Latinx immigrant youth, as well as the community support efforts that are available to this specific group. Using a semi-structured interview on 12 Latinx immigrant youth, this master’s thesis explored: (1) the attitudes towards mental health, (2) attitudes towards mental health services, (3) and the experiences of participating in a local community-based program for Latinx immigrant youth. Results found that most participants (83%) held positive attitudes toward mental health and all participants (100%) described positive attitudes towards mental health services. In addition, all participants reported having positive experiences with a culturally-sensitive youth program that promotes psychological well-being while integrating to the United States and noted that it had benefited them socially, culturally, and psychologically. Given the rise of Latinxs in the U.S., and the stigma surrounding mental health attitudes, it is important to continue to explore this area to better serve Latinx immigrant youth and connect them to mental health services they may need. Also, it is important to have community programs, such as this local youth program, that provide support and meet the needs of Latinx immigrant youth