Studying cetacean behaviour: new technological approaches and conservation applications

Animal behaviour can provide valuable information for wildlife management and conservation. Studying the detailed behaviour of marine mammals involves challenges not faced by most animal behaviour researchers due to the size, mobility and lack of continuous visibility of these animals. We describe several methods developed by marine mammal scientists to study behaviour, primarily of cetaceans, focusing on technological advances: unmanned aerial systems (UAS), satellite-linked telemetry, passive acoustics and multisensor high-resolution acoustic recording tags. We then go on to explain how the data collected by these methods have contributed to and informed conservation actions. We focus on examples including: satellite data informing the interactions between cetaceans and offshore oil and gas development; passive acoustics used to track distributions of several species of cetaceans, including their movements near shipping lanes; and high-resolution acoustic recording tags used to document responses of cetaceans to anthropogenic activities. Finally, we discuss recent efforts to link animal behaviour to individual fitness and, particularly for behavioural disturbances, to population-level consequences, which can be helpful for informing conservation efforts. The infusion of technological advancements into studies of cetacean behaviour combined with emerging analytical techniques brings us to the next 20+ years of studying these animals. These developments will improve our capabilities in areas such as testing whether their behaviour adheres to traditional behavioural theory, and will certainly assist the guiding of conservation efforts

A comparison of baleen whale density estimates derived from overlapping satellite imagery and a shipborne survey

As whales recover from commercial exploitation, they are increasing in abundance in habitats that they have been absent from for decades. However, studying the recovery and habitat use patterns of whales, particularly in remote and inaccessible regions, frequently poses logistical and economic challenges. Here we trial a new approach for measuring whale density in a remote area, using Very-High-Resolution WorldView-3 satellite imagery. This approach has capacity to provide sightings data to complement and assist traditional sightings surveys. We compare at-sea whale density estimates to estimates derived from satellite imagery collected at a similar time, and use suction-cup archival logger data to make an adjustment for surface availability. We demonstrate that satellite imagery can provide useful data on whale occurrence and density. Densities, when unadjusted for surface availability are shown to be considerably lower than those estimated by the ship survey. However, adjusted for surface availability and weather conditions (0.13 whales per km2, CV = 0.38), they fall within an order of magnitude of those derived by traditional line-transect estimates (0.33 whales per km2, CV = 0.09). Satellite surveys represent an exciting development for high-resolution image-based cetacean observation at sea, particularly in inaccessible regions, presenting opportunities for ongoing and future research

The Southern Ocean Exchange: Porous boundaries between humpback whale breeding populations in southern polar waters

Humpback whales (Megaptera novaeangliae) are a cosmopolitan species and perform long annual migrations between low-latitude breeding areas and high-latitude feeding areas. Their breeding populations appear to be spatially and genetically segregated due to long-term, maternally inherited fidelity to natal breeding areas. In the Southern Hemisphere, some humpback whale breeding populations mix in Southern Ocean waters in summer, but very little movement between Pacific and Atlantic waters has been identified to date, suggesting these waters constituted an oceanic boundary between genetically distinct populations. Here, we present new evidence of summer co-occurrence in the West Antarctic Peninsula feeding area of two recovering humpback whale breeding populations from the Atlantic (Brazil) and Pacific (Central and South America). As humpback whale populations recover, observations like this point to the need to revise our perceptions of boundaries between stocks, particularly on high latitude feeding grounds. We suggest that this “Southern Ocean Exchange” may become more frequent as populations recover from commercial whaling and climate change modifies environmental dynamics and humpback whale prey availability

The European Language Portfolio and Teacher Education

The article of record as published may be found at http://dx.doi.org/10.1242/jeb.103259Low-frequency acoustic signals generated by baleen whales can propagate over vast distances, making the assignment of calls to specific individuals problematic. Here, we report the novel use of acoustic recording tags equipped with high-resolution accelerometers to detect vibrations from the surface of two tagged fin whales that directly match the timing of recorded acoustic signals. A tag deployed on a buoy in the vicinity of calling fin whales and a recording from a tag that had just fallen off a whale were able to detect calls acoustically but did not record corresponding accelerometer signals that were measured on calling individuals. Across the hundreds of calls measured on two tagged fin whales, the accelerometer response was generally anisotropic across all three axes, appeared to depend on tag placement and increased with the level of received sound. These data demonstrate that high-sample rate accelerometry can provide important insights into the acoustic behavior of baleen whales that communicate at low frequencies. This method helps identify vocalizing whales, which in turn enables the quantification of call rates, a fundamental component of models used to estimate baleen whale abundance and distribution from passive acoustic monitoring

Integrating remote sensing methods during controlled exposure experiments to quantify group responses of dolphins to navy sonar

Human noise can be harmful to sound-centric marine mammals. Significant research has focused on characterizing behavioral responses of protected cetacean species to navy mid-frequency active sonar (MFAS). Controlled exposure experiments (CEE) using animal-borne tags have proved valuable, but smaller dolphins are not amenable to tagging and groups of interacting individuals are more relevant behavioral units for these social species. To fill key data gaps on group responses of social delphinids that are exposed to navy MFAS in large numbers, we describe novel approaches for the coordinated collection and integrated analysis of multiple remotely-sensed datasets during CEEs. This involves real-time coordination of a sonar source, shore-based group tracking, aerial photogrammetry to measure fine-scale movements and passive acoustics to quantify vocal activity.Using an example CEE involving long-beaked common dolphins ( Delphinus delphis bairdii), we demonstrate how resultant quantitative metrics can be used to estimate behavioral changes and noise exposure-response relationships.</p

Logistics Strategy and Transport Service Choices: An Adaptive Stated Preference Experiment

This paper presents the results of a microanalysis of freight transport demand in a logistics context. Current research concentrates, with few exceptions, on shippers' choice of a transport mode. However, in a global context, shippers' behavior has to be conceived as a complex decision, which considers transport mode choice as only a part of a firm's logistics strategy. Since no data exist to directly estimate the marginal values for different qualities of transport and logistics services, a stated preference approach is applied. Adaptive stated preference experiments were performed for twenty-two firms in Italy and in Switzerland. The experimental results-forty hypothetical binary choices per firm-were completed by background information on the firms' long-term logistics strategies. The results confirm the relevance of the logistics context (e.g., JIT strategies on the supplier's or customer's side) for transport demand. The calculated marginal values of time and characteristics (reliability, frequency, etc.) provide important insights and permit generalized costs in freight transport models to be recalibrated. Copyright 2003 Gatton College of Business and Economics, University of Kentucky..

High-resolution profiling and discovery of planarian small RNAs

Freshwater planarian flatworms possess uncanny regenerative capacities mediated by abundant and collectively totipotent adult stem cells. Key functions of these cells during regeneration and tissue homeostasis have been shown to depend on PIWI, a molecule required for Piwi-interacting RNA (piRNA) expression in planarians. Nevertheless, the full complement of piRNAs and microRNAs (miRNAs) in this organism has yet to be defined. Here we report on the large-scale cloning and sequencing of small RNAs from the planarian Schmidtea mediterranea, yielding altogether millions of sequenced, unique small RNAs. We show that piRNAs are in part organized in genomic clusters and that they share characteristic features with mammalian and fly piRNAs. We further identify 61 novel miRNA genes and thus double the number of known planarian miRNAs. Sequencing, as well as quantitative PCR of small RNAs, uncovered 10 miRNAs enriched in planarian stem cells. These miRNAs are down-regulated in animals in which stem cells have been abrogated by irradiation, and thus constitute miRNAs likely associated with specific stem-cell functions. Altogether, we present the first comprehensive small RNA analysis in animals belonging to the third animal superphylum, the Lophotrochozoa, and single out a number of miRNAs that may function in regeneration. Several of these miRNAs are deeply conserved in animals

Antarctic Futures: An Assessment of Climate-Driven Changes in Ecosystem Structure, Function, and Service Provisioning in the Southern Ocean

In this article, we analyze the impacts of climate change on Antarctic marine ecosystems. Observations demonstrate large-scale changes in the physical variables and circulation of the Southern Ocean driven by warming, stratospheric ozone depletion, and a positive Southern Annular Mode. Alterations in the physical environment are driving change through all levels of Antarctic marine food webs, which differ regionally. The distributions of key species, such as Antarctic krill, are also changing. Differential responses among predators reflect differences in species ecology. The impacts of climate change on Antarctic biodiversity will likely vary for different communities and depend on species range. Coastal communities and those of sub-Antarctic islands, especially range-restricted endemic communities, will likely suffer the greatest negative consequences of climate change. Simultaneously, ecosystem services in the Southern Ocean will likely increase. Such decoupling of ecosystem services and endemic species will require consideration in the management of human activities such as fishing in Antarctic marine ecosystems