O uso do DNA barcoding para identificar barbatanas de tubarão comercializadas ilegalmente no Brasil.

A demanda por barbatanas de tubarão tem aumentado nos últimos anos, estimulando o comércio ilegal e técnicas de captura predatórias que ameaçam a sobrevivência das populações naturais. As barbatanas de tubarão são normalmente removidas imediatamente após a captura e o corpo do animal é jogado de volta ao mar, o que impede a identifi cação morfológica da espécie. Quando a identifi cação morfológica está comprometida, a identifi cação genética pode ser usada para associar amostras desconhecidas com amostras de referência por meio da comparação de sequências de genes mitocondriais. Neste estudo, nós usamos sequências de 650 pares de base da subunidade I do gene citocromo c oxidase (COI) associadas com o Barcode of Life Database (BOLD) para identifi car uma carga de barbatanas de tubarão apreendidas pela Polícia Federal do Brasil em 2011. Nós conseguimos associar com sucesso 25 das 26 amostras encaminhadas para o laboratório com três espécies diferentes, sendo elas Prionace glauca, Isurus oxyrinchus e Sphyrna zygaena. Embora as três espécies não estejam atualmente protegidas pelas leis brasileiras, este estudo reforça a utilidade da ferramenta do DNA barcoding na casuística forense.The demand for shark fi ns has increased in the last years, stimulating the illegal trade and predatory capture techniques that threaten the survival of natural populations. Shark fi ns are usually removed immediately after the catch and the body of the animal is thrown back into the ocean, preventing the morphological identifi cation of the species. When morphological identifi cation is compromised, genetic identifi cation can be used to associate unknown samples to a reference sample by comparing sequences of mitochondrial genes. In this study we used sequences of 650 base pair of the subunit I of cytochrome c oxidase gene (COI) associated with the Barcode of Life Database (BOLD) to identify a cargo of shark fi ns seized by the Brazilian Federal Police in 2011. We have successfully matched 25 of 26 samples sent to the laboratory to three different species, Prionace glauca, Isurus oxyrinchus and Sphyrna zygaena. Although none of them are currently protected by Brazilian laws, this study reinforces the utility of DNA barcoding in forensic casework

"Downhill" varices. A rare cause of esophageal hemorrhage

"Downhill" varices or upper esophageal varices are a rare cause of proximal digestive tract hemorrhage with only 16 cases described in the literature. In our series, hemorrhage due to "downhill" varices represents 0.1% of all acute esophageal variceal bleeding. Their etiology differs from that of the usual "uphill" varices secondary to portal hypertension, and the clinical management should be directed to vascular obstruction if present. We report a case of an 89-year-old male with hemorrhagic "downhill" varices not associated, as usually, with superior vena cava obstruction or compression, but with severe pulmonary hypertension and drug-related hemorrhagic risk factors, whose removal proved sufficient to prevent rebleeding

Anatomical characteristics of the styloid process in internal carotid artery dissection: Case-control study

Introduction Pathophysiology of cervical artery dissection is complex and poorly understood. In addition to well-known causative and predisposing factors, including major trauma and monogenic connective tissue disorders, morphological characteristics of the styloid process have been recently recognized as a possible risk factor for cervical internal carotid artery dissection. Aims To study the association of the anatomical characteristics of styloid process with internal carotid artery dissection. Methods Retrospective, multicenter, case-control study of patients with internal carotid artery dissection and age- and sex-matched controls. Consecutive patients with internal carotid artery dissection and controls with ischemic stroke or transient ischemic attack of any etiology excluding internal carotid artery dissection, who had performed computed tomography angiography, diagnosed between January 2010 and September 2016. Two independent observers measured styloid process length and styloid process distance to internal carotid artery. Results Sixty-two patients with internal carotid artery dissection and 70 controls were included. Interobserver agreement was good for styloid process length and styloid process-internal carotid artery distance (interclass correlation coefficient = 0.89 and 0.76, respectively). Styloid process ipsilateral to dissection was longer than left and right styloid process in controls (35.8 ± 14.4 mm versus 30.4 ± 8.9 mm and 30.3 ± 8.2 mm, p = 0.011 and p = 0.008, respectively). Styloid process-internal carotid artery distance ipsilateral to dissection was shorter than left and right distance in controls (6.3 ± 1.9 mm versus 7.2 ± 2.1 mm and 7.0 ± 2.3 mm, p = 0.003 and p = 0.026, respectively). Internal carotid artery dissection was associated with styloid process length (odds ratio = 1.04 mm-1, 95% confidence interval = 1.01-1.08, p = 0.015) and styloid process-internal carotid artery distance (OR = 0.77 mm-1, 95% confidence interval = 0.64-0.92, p = 0.004). Conclusion Longer styloid process and shorter distance between styloid process and cervical internal carotid artery are associated with cervical internal carotid artery dissection.info:eu-repo/semantics/publishedVersio

Gauge-independent MS‾\overline{MS} renormalization in the 2HDM

We present a consistent renormalization scheme for the CP-conserving Two-Higgs-Doublet Model based on $\overline{MS}$ renormalization of the mixing angles and the soft-$Z_2$-symmetry-breaking scale $M_{sb}$ in the Higgs sector. This scheme requires to treat tadpoles fully consistently in all steps of the calculation in order to provide gauge-independent $S$-matrix elements. We show how bare physical parameters have to be defined and verify the gauge independence of physical quantities by explicit calculations in a general $R_{\xi}$-gauge. The procedure is straightforward and applicable to other models with extended Higgs sectors. In contrast to the proposed scheme, the $\overline{MS}$ renormalization of the mixing angles combined with popular on-shell renormalization schemes gives rise to gauge-dependent results already at the one-loop level. We present explicit results for electroweak NLO corrections to selected processes in the appropriately renormalized Two-Higgs-Doublet Model and in particular discuss their scale dependence.Comment: 52 pages, PDFLaTeX, PDF figures, JHEP version with Eq. (5.23) correcte

A timeband framework for modelling real-time systems

Complex real-time systems must integrate physical processes with digital control, human operation and organisational structures. New scientific foundations are required for specifying, designing and implementing these systems. One key challenge is to cope with the wide range of time scales and dynamics inherent in such systems. To exploit the unique properties of time, with the aim of producing more dependable computer-based systems, it is desirable to explicitly identify distinct time bands in which the system is situated. Such a framework enables the temporal properties and associated dynamic behaviour of existing systems to be described and the requirements for new or modified systems to be specified. A system model based on a finite set of distinct time bands is motivated and developed in this paper

Comfort from the perspective of families of people hospitalized in the intensive care unit

This study aimed at understanding the meaning of comfort to the families of people in intensive care units. It consists of a qualitative study carried out in the intensive care unit of a hospital in Salvador-Bahia. Fourteen family members were interviewed. The authors utilized the theoretical principles of symbolic interactionism and the technique of qualitative data analysis. Results indicated that the categories Safety, Receptiveness, Information, Proximity, Social and Spiritual Support, Convenience and Integration expressed the meaning of comfort, which was comprised of reliability in terms of technical-scientific competence and a supportive and sensitive attitude of the team, chance of recovery, access to information and the opportunity to be close to the patient, support of people in their social life, spiritual sources and the environmental structure of the hospital, preservation of self-care and routine activities. It was concluded that the family is important as objects and subjects of the actions in healthcare and must be the focus in public health policies and programs in Brazil