Evidence that the degree of band 3 phosphorylation modulates human erythrocytes nitric oxide efflux – in vitro model of hyperfibrinogenemia

© 2011 – IOS Press and the authors. All rights reservedRecent evidence has shown that plasma fibrinogen, a major cardiovascular risk factor, interacts with the erythrocyte membrane and acts to influence blood flow via erythrocyte nitric oxide (NO) modulation. In the present pioneer in-vitro study, whole blood samples were harvested from healthy subjects and aliquots were incubated in the absence (control aliquots) and presence of fibrinogen at different degrees of band 3 phosphorylation, and the levels of NO, nitrite, nitrate and S-nitroglutathione (GSNO) were determined. Hyperfibrinogenemia interferes with erythrocyte NO mobilization without changing its efflux in a way that seems to be dependent of the degree of band 3 phosphorylation. In presence of higher fibrinogen concentrations the NO efflux is reinforced when band 3 is phosphorylated (p < 0.001). Higher levels of nitrite, nitrate and GSNO were documented (p < 0.05). However, the mechanisms by which fibrinogen signalling modulates erythrocyte function remain to be clarified and are currently under study. These conditions may be considered an approach to be followed in blood storage for transfusions.This study was supported by grants from the FCT - Fundação para a Ciência e a Tecnologia (project reference PTDC/SAU-OSM/73449/2006

Foraging behavior of juvenile loggerhead sea turtles in the open ocean: from Lévy exploration to area-restricted search

Most sea turtle species spend part of, or their entire juvenile stage in pelagic habitats. A key question is how pelagic turtles exploit their environment to optimize prey intake and max imize fitness. This study combined animal telemetry with remote-sensed environmental data to quantify the drivers and patterns of foraging behavior of juvenile loggerhead sea turtles in the pelagic eastern North Atlantic. Juveniles ranged in size from 34 to 58 cm straight carapace length. First-passage time (FPT) analysis, used to quantify search effort, indicated that turtles performed area-restricted searches at nested spatial scales of 10 and 50 to 200 km. High-usage areas, as quantified by FPT, were associated with increased dive activity and weak surface currents, as well as with oceanographic features (high chlorophyll a and shallower bathymetry) thought to stimu late prey availability. Conversely, low-usage areas (i.e. transit areas) were associated with deep, probably exploratory dives, typical from Lévy movement patterns. Further interpretation of dive data indicates greater dive activity in shallow depths (0 to 10 m) during the night and during tran sit. Conversely, greater activity at intermediate depths (10 to 50 m) was observed during daytime, under strong lunar illumination and in high-usage areas, suggesting these depths are major day time foraging layers. This study clarifies the foraging ecology of sea turtles during their develop ment phase in the open sea, providing evidence that these pelagic predators can adjust their for aging strategies and effort in response to the local conditions of their dynamic environment.info:eu-repo/semantics/publishedVersio

Mechanisms regulating bitter pit development in Greensleeves apples with suppression of ethylene biosynthesis.

The objectives of this study were to understand the role of ethylene and nutrients (Ca2+,Mg2+, K+ and N) on bitter pit (BP) development in wild type (GS) and ethylene suppressed (68G and 103Y) Greensleeves apples. The transgenic line 68G is suppressed for 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) and line 103Y is suppressed for ACC synthase (ACS). Suppression of ethylene biosynthesis reduced BP incidence and severity. Lower ethylene biosynthesis, in ethylene-suppressed genotypes, had no effect on Ca2+, Mg2+, K+ and N concentrations in fruit cortical tissue. In all genotypes, fruit with BP had lower Ca2+ and higher Mg2+ concentrations and higher Mg2+/Ca2+ ratio in cortical tissue. The results indicate that high levels of ethylene biosynthesis and Mg2+ in cortical tissue can enhance fruit susceptibility to BP incidence

Escaldadura superficial em maçãs Granny Smith submetidas ao tratamento térmico com cálcio.

O objetivo deste trabalho foi avaliar o efeito do tratamento térmico combinado com Ca2+ sobre o desenvolvimento de escaldadura superficial em maçãs Granny Smith após o armazenamento refrigerado

Solid-state 13C NMR studies of activated carbons prepared from biomass using different chemical agents.

Activated carbons are largely employed in several chemical and physical processes nowadays, including water treatment, catalysis, gas storage and others [1]. The surface properties of the porous carbons are determinant for most of such applications. Oxygenated functional groups present at the edges of the aromatic lamellae are known to influence decisively the surface chemistry of these materials [2]. In this work, solid-state 13 C nuclear magnetic resonance (NMR) spectroscopy was used for the analysis of a series of activated carbons prepared from a lignocellulosic precursor, using different chemical activating agents

Agronomic Evaluation of Alfalfa Cultivars in Rio Grande do Sul, Brasil

From April 1995 to June 1998, thirty five alfalfa (Medicago sativa L.) cultivars (cvs.) were evaluated in a field trial at the “Depressão Central” ecoclimatic region, in southern Brazil. Two years after sowing, 30 cultivars were excluded from the test due to their poor overall agronomic performance, mainly low forage DM yields. The remaining cultivars: Crioula (local genotype used as a check), Victoria, Rio and P 30 are hay-type cultivars while Alfagraze is a grazing-tolerant type. Twenty-two harvests were made and no significant differences were found for total DM yields among cvs. Crioula, Victoria, Rio and P 30; however, cv. Crioula yielded 25,854 kg.ha-1, showing a significant (P\u3c 0.05) superior performance compared to cv. Alfagraze (17,478 kg.ha-1). The highest average annual and seasonal DM yields were obtained by cv. Crioula and spring was the best growing season. Cv. Alfagraze showed the highest crude protein content and leaf to stem ratio. Incidence of foliar diseases was low, affecting all cultivars similarly. Cv. Victoria showed the lowest stand persistence. Alfalfa root density did not differ among cultivars and the highest average value was found at the 0-10 cm topsoil layer. Results indicate that cvs. Crioula and Alfagraze are well-adapted, productive and persistent alfalfa genotypes for hay production and grazing, respectively, under the soil and climatic conditions of the “Depressão Central” region

Introduction of Forage Legumes into Pastures of Three Different Grasses

It was evaluated the introduction of a mixture of calopo, galaxia, perennial soybean, guata and stylo broadcsted into marandu, setaria and tanzania half-plots already established in three cafeterias, each one located inside a paddock of each grass. The legumes were seeded in the end of the Spring (12/07/98), after the plots had been cut down and fertilized. The cafeterias were grazed intermittently by buffaloes maintained in the three paddocks. The occurrence of the five legume plants was avaluated by countings (#/m2) effected in the middle of Autumn, Winter and Spring of 1999 and Summer of 2000. Along with this last counting it was evaluated the forage remained after grazing and its N%. Marandu-grass presented the higher amount of forage remained after grazing but the lower presence of legumes along all countings. The contrary happened with Tanzania-grass. Significantly higher N% was found in the mixed grass half-plots compared with them without legumes

Effects of a resistance training intervention on the strength-deficit of elite young soccer players

The aim of this study was to examine the effects of a traditional resistance training scheme on the relative strength (RS), relative peak-force (RPF), strength deficit (SDef), and vertical jump and sprint abilities in elite young soccer players. Thirty-five under-20 soccer players from two professional clubs were assessed before and after a 4-week competitive period. One team performed 12 sessions of a structured resistance training program and the other maintained their regular soccer-specific training and competitive routines. The resistance training program consisted of performing half-squat and jump squat exercises at distinct percentages of the one-repetition maximum. Both teams performed pre- and post-measurements in the following order: (1) countermovement jump (CMJ), (2) 20-m sprint, and (3) half-squat one-repetition maximum to determine the RS, RPF, and SDef. A two-way analysis of variance was used to test for group x time interaction among variables. Effect sizes (ES) and 95% confidence intervals (CI) were also calculated. Group x time interactions were demonstrated for RS ([ES [95%CI] = 1.21 [0.57; 1.85], P = 0.001), RPF (ES [95%CI] = 1.18 [0.52; 1.80], P = 0.001), SDef (ES [95%CI] = 0.86 [0.01; 1.71], P = 0.04), and CMJ (ES [95%CI] = 0.64 [0.28; 0.99], P = 0.001); whereas a non-significant interaction was observed for 20-m sprint performance (ES [95%CI] = 0.02 [-0.32; 0.36], P = 0.85). Traditional strength-power oriented training resulted in improved maximum strength performance and CMJ ability but, paradoxically, increased the SDef. As a consequence, stronger athletes are not necessarily able to use greater percentages of their peak-force against relatively lighter loads