96 research outputs found

    Geologically constrained evolutionary geomechanical modelling of diapir and basin evolution: a case study from the Tarfaya basin, West African coast

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    We systematically incorporate burial history, sea floor geometry and tectonic loads from a sequential kinematic restoration model into a 2D evolutionary geomechanical model that simulates the formation of the Sandia salt diapir, Tarfaya basin, NW African Coast. We use a poro-elastoplastic description for the sediment behaviour and a viscoplastic description for the salt. Sedimentation is coupled with salt flow and regional shortening to determine the sediment porosity and strength and to capture the interaction between salt and sediments. We find that temporal and spatial variation in sedimentation rate is a key control on the kinematic evolution of the salt system. Incorporation of sedimentation rates from the kinematic restoration at a location east of Sandia leads to a final geomechanical model geometry very similar to that observed in seismic reflection data. We also find that changes in the variation of shortening rates can significantly affect the present-day stress state above salt. Overall, incorporating kinematic restoration data into evolutionary models provides insights into the key parameters that control the evolution of geologic systems. Furthermore, it enables more realistic evolutionary geomechanical models, which, in turn, provide insights into sediment stress and porosity

    Intersectin associates with synapsin and regulates its nanoscale localization and function.

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    Neurotransmission is mediated by the exocytic release of neurotransmitters from readily releasable synaptic vesicles (SVs) at the active zone. To sustain neurotransmission during periods of elevated activity, release-ready vesicles need to be replenished from the reserve pool of SVs. The SV-associated synapsins are crucial for maintaining this reserve pool and regulate the mobilization of reserve pool SVs. How replenishment of release-ready SVs from the reserve pool is regulated and which other factors cooperate with synapsins in this process is unknown. Here we identify the endocytic multidomain scaffold protein intersectin as an important regulator of SV replenishment at hippocampal synapses. We found that intersectin directly associates with synapsin I through its Src-homology 3 A domain, and this association is regulated by an intramolecular switch within intersectin 1. Deletion of intersectin 1/2 in mice alters the presynaptic nanoscale distribution of synapsin I and causes defects in sustained neurotransmission due to defective SV replenishment. These phenotypes were rescued by wild-type intersectin 1 but not by a locked mutant of intersectin 1. Our data reveal intersectin as an autoinhibited scaffold that serves as a molecular linker between the synapsin-dependent reserve pool and the presynaptic endocytosis machinery

    Ketahanan Sumberdaya Genetik Jagung Sulawesi Tenggara terhadap Cekaman Kekeringan pada Berbagai Fase Vegetatif

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    Maize crops experiencing water stress can experience cell damage, loss of turgor, closed stomata, plant leaf roll then wilt. Germination and vegetative growth are thought to be a very sensitive phases in relation to the availability of water, because it can influence subsequent growth processes. This study aimed to determine the potential tolerance of Southeast Sulawesi\u27s maize genotypes to drought stress at different vegetative growth phases. This study was based on completely randomized design (CRD) with factorial pattern consisting of two factors: the first factor composed of 9 local maize genotypes of Southeast Sulawesi and 1 national variety (cv. Arjuna), while the second factor was drought stress at different vegetative growth phases, consisting of four levels ie: C0 = plants irrigated with 100 % water availability during the growth phase, C1 = Stress for 5 days , at 21-26 days old (vegetative phase), C2 = Stress for 5 days starting at panicle emergence (early flowering stage), and C3 = Stress for 5 days starting 2 weeks after silking. Research results showed that Genotype (G) treatment significantly influenced all observed growth variables (at age 21 and 42 days after planting, DAP), except for the variable of number of leaf, age 21 DAP. However, water stress treatment (C) only significantly affected plant height variable, at the age of 42 DAP. In general, G6 and G7 genotypes tended to have a higher crop and trunk diameter than the other genotypes. Contrary, G3 genotype tended to have shorter crop and smaller stem diameter than the other genotypes. There are indications that the drought stress treatment (C) significantly inhibited the growth of maize crops

    Morphological characterization of bushy cells and their inputs in the laboratory mouse (Mus musculus) anteroventral cochlear nucleus.

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    PMC3753269Spherical and globular bushy cells of the AVCN receive huge auditory nerve endings specialized for high fidelity neural transmission in response to acoustic events. Recent studies in mice and other rodent species suggest that the distinction between bushy cell subtypes is not always straightforward. We conducted a systematic investigation of mouse bushy cells along the rostral-caudal axis in an effort to understand the morphological variation that gives rise to reported response properties in mice. We combined quantitative light and electron microscopy to investigate variations in cell morphology, immunostaining, and the distribution of primary and non-primary synaptic inputs along the rostral-caudal axis. Overall, large regional differences in bushy cell characteristics were not found; however, rostral bushy cells received a different complement of axosomatic input compared to caudal bushy cells. The percentage of primary auditory nerve terminals was larger in caudal AVCN, whereas non-primary excitatory and inhibitory inputs were more common in rostral AVCN. Other ultrastructural characteristics of primary auditory nerve inputs were similar across the rostral and caudal AVCN. Cross sectional area, postsynaptic density length and curvature, and mitochondrial volume fraction were similar for axosomatic auditory nerve terminals, although rostral auditory nerve terminals contained a greater concentration of synaptic vesicles near the postsynaptic densities. These data demonstrate regional differences in synaptic organization of inputs to mouse bushy cells rather than the morphological characteristic of the cells themselves.JH Libraries Open Access Fun

    Large-Scale Three-Dimensional Geomechanical Modeling of Reservoirs: Examples from California and the Deepwater Gulf of Mexico

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    Cost-effective improvements in the technology needed to develop and manage reservoirs in challenging environments require an increase in our understanding of geomechanical behavior. The local stresses relevant to reservoir-scale processes are strongly affected by stratigraphic and structural features at that scale, and the relationship between hydrocarbon production and the mechanical behavior of the reservoir and/or overburden can be complicated and difficult to discern from field data directly. Numerical simulation provides a means to achieve critical insight into the behavior of complex geosystems and advance understanding of both the subsurface environment before drilling as well as the relationship between fluid flow and geomechanical behavior during production. This paper reviews our recent work aimed at utilizing large-scale geomechanical simulation as a reservoir management tool. We first describe the constitutive models developed specifically for two important classes of geomaterials that are implemented in the quasi-static largedeformation finite element code JAS3D that we use in our work. We then describe several field cases where we apply nonlinear finite element modeling to key problems in reservoir mechanics. The first field cases involve historical geomechanical simulations of primary and secondary recovery at the Belridge Diatomite and Lost Hills fields located in California's San Joaquin Valley. In this work, we apply nonlinear finite element modeling to investigate the causes of well casing damage experienced by the field operators and to identify mitigation strategies. Next, we describe an application that addresses potential well integrity issues associated with sub-salt and near-salt deepwater Gulf of Mexico reservoirs. In this work, we analyze hole closure behavior and quantify loading on casings for wells that penetrate thick salt formations to ensure that wells are designed to withstand salt loading over a service lifetime of 20-30 years. The field studies illustrate the difficult issues encountered in practical applications of large-scale three-dimensional nonlinear finite element geomechanical modeling and suggest areas where research advances could be beneficial. These areas include: development of solids models and finite element meshes from disparate geologic and/or numerical data, development of realistic constitutive models, robust and efficient implementation of these material models in finite element codes to achieve reasonable solution times, experimental rock mechanics data and the natural heterogeneity of geosystems, implementation of far-field (tectonic) stresses and stress initialization, and integration of geomechanical modeling results with other analysis tools

    Charge transfer processes and UV induced absorption in Yb:YAG single crystal laser materials

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    Charge transfer (CT) transitions and UV induced color centers in Yb:YAG single crystals have been investigated. A simultaneous pair formation of a stable Yb2+ ion and a hole related (O -) color center (hole polaron) are observed through a CT-process. Slightly different types of hole related color centers are formed in Yb:YAG crystals containing small levels of iron impurities. Furthermore, excitation spectroscopy on the UV irradiated Yb:YAG samples could confirm an energy transfer process between Yb3+ and Yb2+ ions. The findings are important for an increased knowledge of the physical loss mechanisms observed in Yb-doped laser materials, such as the nonlinear decay process in Yb:YAG crystals as well as the photodarkening phenomenon in Yb-doped fiber lasers
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