Influence of magnetic nanoparticle degradation in the frame of magnetic hyperthermia and photothermal treatments

This work aims at studying how the transformations that magnetic nanoparticles suffer in vivo affect their heating properties in the frame of hyperthermia treatments. Iron oxide magnetic nanoparticles (≈13 nm) with two different coatings [PMAO (polymaleic anhydride-alt-1-octadecene) and DMSA (dimercaptosuccinic acid)] have been subjected to an accelerated degradation in a medium simulating lysosome conditions. The particles physicochemical properties (size, size distribution, and magnetic properties) have been followed over the degradation process along 24 days. It was found that DMSA-coated particles degraded much faster than PMAO-coated ones. In addition, their heating properties under both the exposure to an alternating magnetic field or a near infrared light have been tracked along this degradation processes, assessing how the changes in their physicochemical properties affect their heating capacity. Along the degradation procedure, a stronger decrease of the particles heating properties has been observed in the frame of magnetic hyperthermia measurements, in comparison with the photothermal ones. Finally, the PMAO-coated particles have been selected for a degradation study in vivo after intratumoral administration. Interestingly, although the number of particles decreases with time in the tissue, the size and size distribution of the particles do not change significantly over time. This work is especially relevant in the frame of the design of in vivo hyperthermia treatments using magnetic nanoparticles as it would provide fundamental clues regarding the need of repeated doses or the possible use of a single administration depending on the treatment duration

Fate and transformation of silver nanoparticles in different biological conditions

The exploitation of silver nanoparticles (AgNPs) in biomedicine represents more than one third of their overall application. Despite their wide use and significant amount of scientific data on their effects on biological systems, detailed insight into their in vivo fate is still lacking. This study aimed to elucidate the biotransformation patterns of AgNPs following oral administration. Colloidal stability, biochemical transformation, dissolution, and degradation behaviour of different types of AgNPs were evaluated in systems modelled to represent biological environments relevant for oral administration, as well as in cell culture media and tissue compartments obtained from animal models. A multimethod approach was employed by implementing light scattering (dynamic and electrophoretic) techniques, spectroscopy (UV–vis, atomic absorption, nuclear magnetic resonance) and transmission electron microscopy. The obtained results demonstrated that AgNPs may transform very quickly during their journey through different biological conditions. They are able to degrade to an ionic form and again reconstruct to a nanoparticulate form, depending on the biological environment determined by specific body compartments. As suggested for other inorganic nanoparticles by other research groups, AgNPs fail to preserve their specific integrity in in vivo settings

From monomeric to multimeric His-tag proteins conjugation to magnetic nanoparticles through NTA-Me2+: shape and size effects

Resumen del póster presentado a la 4th Spanish Conference on Biomedical Applications of Nanomaterials, celebrda online del 2 al 4 de junio de 2021.Peer reviewe

Dependence of intracellular magnetic nanoparticles amount in the apoptotic death pathway triggered by magnetic hyperthermia treatment

Trabajo presentado a la 4th Spanish Conference on Biomedical Applications of Nanomaterials, celebrada online del 2 al 4 de junio de 2021.Peer reviewe

Synthesis and functionalization of magnetic nanoparticles for magnetic hyperthermia applications

Resumen del trabajo presentado al Molecular Horizons Seminar de la University of Wollongong (Australia) celebrado el 3 de octubre de 2019

Localized hyperthermia as a tool for cell membrane hyperfluidization

Resumen del trabajo presentado al 20th International Union of Materials Research Societies - International Conference of Asia (IUMRS-ICA), celebrada en Perth (Australia) del 22 al 26 de septiembre de 2019.European Union (Marie Sklodowska-Curie grant agreement No. 657215; MERA. NET COFUND 2016, PCIN-2017-060) and MINECO (Ramon y Cajal grant RYC-2015-17640)

Síntesis y funcionalización de nanopartículas magnéticas para aplicaciones biomédicas

Resumen del trabajo presentado al Ciclo de Conferencias del Departamento de Química Orgánica de la Universidad del País Vasco, celebrado el 25 de febrero de 2019

Química bioortogonal en Nanotecnología. Desafíos y oportunidades

Trabajo presentado al Congreso Iberoamericano de Química: "85 Años de la Sociedad Química del Perú" y al XXIX Congreso Peruano de Química, celebrados en Lima (Perú) del 16 al 19 de octubre de 2018.Peer Reviewe

Localized magnetic nanoheating - generating hotspots with iron oxide- based nanoparticles

Resumen del trabajo presentado al 20th International Union of Materials Research Societies - International Conference of Asia (IUMRS-ICA), celebrada en Perth (Australia) del 22 al 26 de septiembre de 2019.Peer reviewe