Infusing a Course-Based Undergraduate Research Experience (CURE) into an Allied Health Curriculum

Purpose: Infusion of a course-based undergraduate research experience (CURE) into an existing research design course in an applied science curriculum allowed medical laboratory science students (n=22) to each be a contributing team member in a hand’s-on research experience, where most of the work was completed during the class time on campus. This design allowed for equal access, an equitable experience, and inclusion of all students enrolled in the course. Methods: Students and instructors worked together to develop a research question. The group agreed that the research question would be to determine the number of environmental specimens that were positive for mycobacteria species in residential plumbing specimens from different faucets and showerheads within residences in local areas. Before the actual collection of specimens, students reviewed the literature and completed more traditional modules in research ethics and Collaborative Institutional Training Initiative (CITI) training. Once that was completed, students designed and assembled the collection kits, collected and processed the specimens, and reported their results. Results: Students completed most tasks during the designated class time, and those tasks that had to be completed outside of class were not overwhelming for the students either in time or effort. The students’ reflections as the human subjects in this CURE indicated that 1) 90% of the students agreed they had a better understanding of the Institutional Review Board (IRB) process, 2) 100% of the students agreed the collection process was easily completed, 3) 100% of the students agreed the specimen testing was easily completed and interpreted, and 4) 100% of the students agreed the required parameters of a CURE were met. Conclusion: A CURE can be infused successfully into an applied science course allowing every student to become a contributing member of the research team

Novel Structural Components of the Ventral Disc and Lateral Crest in Giardia intestinalis

Giardia intestinalis is a ubiquitous parasitic protist that is the causative agent of giardiasis, one of the most common protozoan diarrheal diseases in the world. Giardia trophozoites attach to the intestinal epithelium using a specialized and elaborate microtubule structure, the ventral disc. Surrounding the ventral disc is a less characterized putatively contractile structure, the lateral crest, which forms a continuous perimeter seal with the substrate. A better understanding of ventral disc and lateral crest structure, conformational dynamics, and biogenesis is critical for understanding the mechanism of giardial attachment to the host. To determine the components comprising the ventral disc and lateral crest, we used shotgun proteomics to identify proteins in a preparation of isolated ventral discs. Candidate disc-associated proteins, or DAPs, were GFP-tagged using a ligation-independent high-throughput cloning method. Based on disc localization, we identified eighteen novel DAPs, which more than doubles the number of known disc-associated proteins. Ten of the novel DAPs are associated with the lateral crest or outer edge of the disc, and are the first confirmed components of this structure. Using Fluorescence Recovery After Photobleaching (FRAP) with representative novel DAP::GFP strains we found that the newly identified DAPs tested did not recover after photobleaching and are therefore structural components of the ventral disc or lateral crest. Functional analyses of the novel DAPs will be central toward understanding the mechanism of ventral disc-mediated attachment and the mechanism of disc biogenesis during cell division. Since attachment of Giardia to the intestine via the ventral disc is essential for pathogenesis, it is possible that some proteins comprising the disc could be potential drug targets if their loss or disruption interfered with disc biogenesis or function, preventing attachment

“Stick ‘n’ peel”: Explaining unusual patterns of disarticulation and loss of completeness in fossil vertebrates

Few fossil vertebrate skeletons are complete and fully articulated. Various taphonomic processes reduce the skeletal fidelity of decaying carcasses, the effects of most of which are reasonably well understood. Some fossil vertebrates, however, exhibit patterns of disarticulation and loss of completeness that are difficult to explain. Such skeletons are one of two variants. They are incomplete, often markedly so, but the preserved parts are highly articulated. Alternatively, they are complete, or nearly so, but articulation varies markedly between parts of the body. A characteristic feature is the absence of skeletal elements that, on the basis of their larger size and/or greater density, would be predicted to be present. Here we erect a model, termed “stick ‘n’ peel”, that explains how these distinctive patterns originate. The model emphasizes the role of decay products, especially fluids released from the carcass while resting on the sediment surface. These fluids permeate the sediment below and around the carcass. As a result, skeletal elements on the downward facing side of the carcass become adhered to the sediment surface, and are less likely to be remobilized as a result of current activity than others. The pattern of articulation and, especially, completeness is thus not what would be predicted on the basis of the size, shape and density of the skeletal elements. The effects of stick ‘n’ peel are difficult to predict a priori. Stick ‘n’ peel has been identified in vertebrate fossils in lacustrine and marine settings and is likely to be a common feature of the taphonomic history of many vertebrate assemblages. Specimens becoming adhered to the substrate may also explain the preservation in situ of the multi-element skeletons of invertebrates such as echinoderms, and integumentary structures such as hair and feathers in exceptionally preserved fossils