Remotely Controlled Diffusion from Magnetic Liposome Microgels

The reversible, temperature-dependent change in the permeability of a phospholipid bilayer has been used for controlling the diffusion rate of encapsulated molecular payload from liposomes. Liposomes were preloaded with a fluorescent dye and immobilized in calcium alginate hydrogel microparticles that also contained iron oxide nanoparticles. The composite microparticles were produced by a drop-on-demand inkjet method. The ability of iron oxide nanoparticles to locally dissipate heat upon exposure to a radio-frequency (RF) alternating magnetic field was used to control the local temperature and therefore diffusion from the liposomes in a contactless way using an RF coil. Several different release patterns were realized, including repeated on-demand release. The internal structure of the composite alginate–liposome–magnetite microparticles was investigated, and the influence of microparticle concentration on the heating rate was determined. In order to achieve a temperature rise required for the liposome membrane melting, the concentration of alginate beads should be at least 25% of their maximum packing density for the nanoparticle concentration and specific absorption rate used

Influence of electrically conductive adhesive amount on shear strength of glued joints

This paper deals with the influence of electrically conductive adhesive amount on shear strength of joints glued by EPO-TEK® H20S and MG 8331S. These joints were made by gluing of chip resistors 1206, 0805 and 0603 with two curing profiles for each adhesive. The different thickness of stencils and reductions of the hole in stencils were used. These differences have an effect on the amount of conductive adhesives on the samples. The curing profiles and various amounts of the adhesives have an effect on the mechanical strength of the joint. Samples were measured after curing process by using shear strength test with the device LabTest 3.030

Advanced technology for grinding of aluminium alloys parts

In the presents the aluminium and its alloys is doing high advanced technical materials whose proprieties use in the automotive and aerospace industry. In the production of products is effort of producer to production of parts quality and machine with acceptable price. The machining is making important portion by production of the machine parts of automotive industry. The long-terms opinions on the grinding of aluminium alloys in relation to lost of cutting power (glued up of wheel and impaction of pores) important terms there are not so use for aluminium alloys grinding and surface quality. The competition of grinding to advanced production method e.g. forming and turning with advanced tools do not threat dominance of these methods. The use of grinding we are finding in the minor series of production where the use of CNC and forming machines is not suitable in view point of price. The customer requirement of quality (roughness and geometrical accuracy) by grinding of aluminium alloys is guaranteed and correspond with normal and precision grinding e.g. for steels and hardened steels

Methodology for Experimental Analysis of Pipeline System Vibration

AbstractThere are presented two methods used for an investigation of modal parameters, concretely natural frequencies and modal shapes of a significantly simplified model of compressor yard pipeline system in this article. The first one is an experimental method and uses experimental modal analysis for that purpose with using of system Pulse6. The second method is finite element method and was performed in software Solid Works. The pipeline was built in on one side and elastically supported on the other side. The modal parameters of a pipeline system were evaluated for two cases. First one where springs of pipeline support were not preloaded and second one where preload was applied

Fire tests on beams with class 4 cross-section subjected to lateral torsional buckling

This paper describes experimental research in behaviour of laterally unrestrained beams (I or H section) of Class 4 constant or variable cross-sections at elevated temperatures. Preparation and design of experiments is described. The design of the test set-up was made by FE modelling and the experiments followed. The test results are given. Future numerical investigation is planned for full understanding of the fire behaviour of steel members of Class 4 cross-sections considering both welded and hot-rolled I or H shape steel profiles

Fungal CSL transcription factors

<p>Abstract</p> <p>Background</p> <p>The CSL (CBF1/RBP-Jκ/Suppressor of Hairless/LAG-1) transcription factor family members are well-known components of the transmembrane receptor Notch signaling pathway, which plays a critical role in metazoan development. They function as context-dependent activators or repressors of transcription of their responsive genes, the promoters of which harbor the GTG(G/A)GAA consensus elements. Recently, several studies described Notch-independent activities of the CSL proteins.</p> <p>Results</p> <p>We have identified putative CSL genes in several fungal species, showing that this family is not confined to metazoans. We have analyzed their sequence conservation and identified the presence of well-defined domains typical of genuine CSL proteins. Furthermore, we have shown that the candidate fungal protein sequences contain highly conserved regions known to be required for sequence-specific DNA binding in their metazoan counterparts. The phylogenetic analysis of the newly identified fungal CSL proteins revealed the existence of two distinct classes, both of which are present in all the species studied.</p> <p>Conclusion</p> <p>Our findings support the evolutionary origin of the CSL transcription factor family in the last common ancestor of fungi and metazoans. We hypothesize that the ancestral CSL function involved DNA binding and Notch-independent regulation of transcription and that this function may still be shared, to a certain degree, by the present CSL family members from both fungi and metazoans.</p