Nonparametric Regression Estimation for Circular Data

[Abstract] Non-parametric regression with a circular response variable and a unidimensional linear regressor is a topic which was discussed in the literature. In this work, we extend the results to the case of multivariate linear explanatory variables. Nonparametric procedures to estimate the circular regression function are formulated. A simulation study is carried out to study the sample performance of the proposed estimators.Ministerio de Economía y Competitividad; MTM2016-76969-PMinisterio de Economía y Competitividad; MTM2017-82724-RXunta de Galicia; ED481A-2017/361Grupos de Referencia Competitiva; ED431C-2016-015Centro Singular de Investigación de Galicia; ED431G/0

Nonparametric multiple regression estimation for circular response

Versión final aceptada de: https://doi.org/10.1007/s11749-020-00736-wThis version of the article has been accepted for publication, after peer review and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect postacceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s11749-020-00736-wNonparametric estimators of a regression function with circular response and -valued predictor are considered in this work. Local polynomial estimators are proposed and studied. Expressions for the asymptotic conditional bias and variance of these estimators are derived, and some guidelines to select asymptotically optimal local bandwidth matrices are also provided. The finite sample behavior of the proposed estimators is assessed through simulations, and their performance is also illustrated with a real data set.The authors acknowledge the support from the Xunta de Galicia Grant ED481A-2017/361 and the European Union (European Social Fund—ESF). This research has been partially supported by MINECO Grants MTM2016-76969-P and MTM2017-82724-R, and by the Xunta de Galicia (Grupo de Referencia Competitiva ED431C-2017-38, and Centro de Investigación de Galicia “CITIC” ED431G 2019/01), all of them through the ERDF. The authors thank Prof. Felicita Scapini and his research team who kindly provided the sand hoppers data that are used in this work. Data were collected within the Project ERB ICI8-CT98-0270 from the European Commission, Directorate General XII Science. The authors also thank two anonymous referees for numerous useful comments that significantly improved this article.Xunta de Galicia; ED481A-2017/361Xunta de Galicia; ED431C-2017-38Xunta de Galicia; ED431G 2019/0

Satellitome analysis of Rhodnius prolixus, one of the main Chagas disease vector species

The triatomine Rhodnius prolixus is the main vector of Chagas disease in countries such as Colombia and Venezuela, and the first kissing bug whose genome has been sequenced and assembled. In the repetitive genome fraction (repeatome) of this species, the transposable elements represented 19% of R. prolixus genome, being mostly DNA transposon (Class II elements). However, scarce information has been published regarding another important repeated DNA fraction, the satellite DNA (satDNA), or satellitome. Here, we offer, for the first time, extended data about satellite DNA families in the R. prolixus genome using bioinformatics pipeline based on low-coverage sequencing data. The satellitome of R. prolixus represents 8% of the total genome and it is composed by 39 satDNA families, including four satDNA families that are shared with Triatoma infestans, as well as telomeric (TTAGG)n and (GATA)n repeats, also present in the T. infestans genome. Only three of them exceed 1% of the genome. Chromosomal hybridization with these satDNA probes showed dispersed signals over the euchromatin of all chromosomes, both in autosomes and sex chromosomes. Moreover, clustering analysis revealed that most abundant satDNA families configured several superclusters, indicating that R. prolixus satellitome is complex and that the four most abundant satDNA families are composed by different subfamilies. Additionally, transcription of satDNA families was analyzed in different tissues, showing that 33 out of 39 satDNA families are transcribed in four different patterns of expression across samples

Numerical and experimental analyses of biocomposites reinforced with natural fibres

n the last decades the biocomposites have been widely used in the construction, automobile and aerospace industries. Not only the interface transition zone (ITZ) but also the heterogeneity of natural fibres affects the mechanical behaviour of these composites. This work focuses on the numerical and experimental analyses of a polymeric composite fabricated with epoxy resin and unidirectional sisal and banana fibres. A three-dimensional model was set to analyze the composites using the elastic properties of the individual phases. In addition, a two-dimensional model was set taking into account the effective composite properties obtained by micromechanical models. A tensile testing was performed to validate the numerical analyses and evaluating the interface condition of the constitutive phases

Multidisciplinary approach detects speciation within the kissing bug Panstrongylus rufotuberculatus populations (Hemiptera, Heteroptera, Reduviidae)

BACKGROUND Panstrongylus rufotuberculatus (Hemiptera-Reduviidae) is a triatomine species with a wide geographic distribution and a broad phenotypic variability. In some countries, this species is found infesting and colonising domiciliary ecotopes representing an epidemiological risk factor as a vector of Trypanosoma cruzi, etiological agent of Chagas disease. In spite of this, little is known about P. rufotuberculatus genetic diversity. METHODS Cytogenetic studies and DNA sequence analyses of one nuclear (ITS-2) and two mitochondrial DNA sequences (cyt b and coI) were carried out in P. rufotuberculatus individuals collected in Bolivia, Colombia, Ecuador and Mexico. Moreover, a geometric morphometrics study was applied to Bolivian, Colombian, Ecuadorian and French Guiana samples. OBJECTIVES To explore the genetic and phenetic diversity of P. rufotuberculatus from different countries, combining chromosomal studies, DNA sequence analyses and geometric morphometric comparisons. FINDINGS We found two chromosomal groups differentiated by the number of X chromosomes and the chromosomal position of the ribosomal DNA clusters. In concordance, two main morphometric profiles were detected, clearly separating the Bolivian sample from the other ones. Phylogenetic DNA analyses showed that both chromosomal groups were closely related to each other and clearly separated from the remaining Panstrongylus species. High nucleotide divergence of cyt b and coI fragments were observed among P. rufotuberculatus samples from Bolivia, Colombia, Ecuador and Mexico (Kimura 2-parameter distances higher than 9%). MAIN CONCLUSIONS Chromosomal and molecular analyses supported that the two chromosomal groups could represent different closely related species. We propose that Bolivian individuals constitute a new Panstrongylus species, being necessary a detailed morphological study for its formal description. The clear morphometric discrimination based on the wing venation pattern suggests such morphological description might be conclusive.MEC-DICYT: II/FVF/2019/054CSIC: No. 16

Seismic Monitoring Tool for Tidal-Forced Aquifer Level Changes in the Río de la Plata Coastal Plain, Argentina

Ambient seismic noise has gained extensive applications in seismology and plays a pivotal role in environmental seismic studies. This study focuses on the Río de la Plata Coastal Plain, employing the horizontal-to-vertical spectral ratio (HVSR) method on ambient seismic noise records to analyze subsurface dynamics. The region’s hydrogeology is complex, featuring partially interconnected coastal aquifers. The HVSR analysis reveals two peaks, with P0 associated with the sediment-basement interface and P1 linked to a shallower stratigraphic discontinuity. Temporal analysis of P1 highlights cyclical patterns correlated with estuarine levels, suggesting a relationship between variations in seismic velocities and tidal dynamics. Comparisons with aquifer data support the hypothesis that tidal variations influence subsurface mechanical properties, impacting the HVSR function. The study hints at the potential of ambient seismic noise analysis as a non-invasive and cost-effective method for studying coastal aquifers and understanding groundwater dynamics. Ongoing research aims to further explore these relationships for enhanced groundwater resource management

Holocentric chromosome evolution in kissing bugs (Hemiptera: Reduviidae: Triatominae): diversification of repeated sequences

Background: The analysis of the chromosomal and genome evolution in organisms with holocentric chromosomes is restricted by the lack of primary constriction or centromere. An interesting group is the hemipteran subfamily Triatominae, vectors of Chagas disease, which affects around 6 to 7 million people worldwide. This group exhibits extensive variability in the number and chromosomal location of repeated sequences such as heterochromatin and ribosomal genes. This paper tries to reveal the significant differences of the repeated sequences among Triatoma species through the use of genomic DNA probes. Methods: We analysed the chromosomal distribution and evolution of repeated sequences in Triatoma species by genomic in situ hybridization (GISH) using genomic DNA probes from two North American Triatoma species. These genomic probes were hybridized both on their own chromosomes and on other Triatoma species from North and South America, with different amounts and chromosome location of C-heterochromatin. The results were compared with those previously described using South American Triatoma genomic probes. Results: We observed two chromosomal hybridization patterns: (i) very intense hybridization signals concentrated on specific chromosomal regions or particular chromosomes; and (ii) lower intensity hybridization signals dispersed along all chromosomes. Self-GISH on T. rubrofasciata and T. dimidiata chromosomes presented strong hybridization signals on all C-heterochromatin regions. However, when we perform genomic cross-hybridizations, only strong signals are detected on the Y chromosome, leaving the C-heterochromatic autosomal regions unmarked. Conclusions: We confirm that repeated DNA of the Y chromosome is shared among Triatoma species and probably represents an ancestral character of the Triatomini tribe. On the contrary, autosomal heterochromatic regions are constituted by species-specific DNA repeats, most probably satDNA families, suggesting that Triatoma speciation involved the amplification of diverse types of autosomal repeats. Molecular characterization of principal repetitive DNAs seems to be an appropriate approach to infer evolutionary relationships in triatomines