Ultrathin cellulose ester films: preparation, characterization and protein immobilization

In this study cellulose acetate butyrate (CAB) and carboxymehtylcellulose acetate butyrate (CMCAB) films adsorbed onto silicon wafers were characterized by means of ellipsometry, atomic force microscopy (AFM), sum frequency generation spectroscopy (SFG) and contact angle measurements. The adsorption behavior of lysozyme (LIS) or bovine serum albumin (BSA) onto CAB and CMCAB films was investigated. The amounts of adsorbed LIS or BSA onto CMCAB films were more pronounced than those onto CAB films due to the presence of carboxymethyl group in the CMCAB structure. Besides, the adsorption of BSA molecules on CMCAB films was more favored than that of LIS molecules. Antimicrobial effect of LIS bound to CAB or CMCAB layers was evaluated using Micrococcus luteus as substrate.FAPESPCNPqCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES

Proposed Steps to Projects Development in the Public Sector

Public Policy and projects have been the subject of discussion in public organizations, with the argument that it organizations has resources, but there are no projects to invest. This research aims to suggest a step-by-step proposal to elaborate a project, based on a public policy, in a way that will improve public management, with specific results and contribute to the quality of life of citizens and society. In this research was analyzed the concepts of public management, public policy, programs and projects, which resulted in a logical, interrelated proposal, consisting of steps that comprise a step by step to elaborate project to a public policy. He sought the qualitative aspect and used the bibliographical research as methodology. Structuring the work in chapters organizing in introduction, theoretical survey and its analysis, proposed a step by step to elaborate project and concluded with the final considerations

Counter-regulatory Phosphatases Tnap And Npp1 Temporally Regulate Tooth Root Cementogenesis

Cementum is critical for anchoring the insertion of periodontal ligament fibers to the tooth root. Several aspects of cementogenesis remain unclear, including differences between acellular cementum and cellular cementum, and between cementum and bone. Biomineralization is regulated by the ratio of inorganic phosphate (P-i) to mineral inhibitor pyrophosphate (PPi), where local P-i and PPi concentrations are controlled by phosphatases including tissue-nonspecific alkaline phosphatase (TNAP) and ectonucleotide pyrophosphatase/phosphodiesterase 1 (NPP1). The focus of this study was to define the roles of these phosphatases in cementogenesis. TNAP was associated with earliest cementoblasts near forming acellular and cellular cementum. With loss of TNAP in the Alpl null mouse, acellular cementum was inhibited, while cellular cementum production increased, albeit as hypomineralized cementoid. In contrast, NPP1 was detected in cementoblasts after acellular cementum formation, and at low levels around cellular cementum. Loss of NPP1 in the Enpp1 null mouse increased acellular cementum, with little effect on cellular cementum. Developmental patterns were recapitulated in a mouse model for acellular cementum regeneration, with early TNAP expression and later NPP1 expression. In vitro, cementoblasts expressed Alpl gene/protein early, whereas Enpp1 gene/protein expression was significantly induced only under mineralization conditions. These patterns were confirmed in human teeth, including widespread TNAP, and NPP1 restricted to cementoblasts lining acellular cementum. These studies suggest that early TNAP expression creates a low PPi environment promoting acellular cementum initiation, while later NPP1 expression increases PPi, restricting acellular cementum apposition. Alterations in PPi have little effect on cellular cementum formation, though matrix mineralization is affected.712741Intramural Research Program of the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) of the National Institutes of Health (NIH

Breakdown of smoothness for the Muskat problem

In this paper we show that there exist analytic initial data in the stable regime for the Muskat problem such that the solution turns to the unstable regime and later breaks down i.e. no longer belongs to $C^4$.Comment: 93 pages, 10 figures (6 added

Lack of uniqueness for weak solutions of the incompressible porous media equation

In this work we consider weak solutions of the incompressible 2-D porous media equation. By using the approach of De Lellis-Sz\'ekelyhidi we prove non-uniqueness for solutions in $L^\infty$ in space and time.Comment: 23 pages, 2 fugure

Age and regional differences in clinical presentation and risk of hospitalization for dengue in Brazil, 2000-2014

OBJECTIVES: Dengue cases range from asymptomatic to severe, eventually leading to hospitalization and death. Timely and appropriate management is critical to reduce morbidity. Since 1980, dengue has spread throughout Brazil, affecting an increasing number of individuals. This paper describes age and regional differences in dengue’s clinical presentation and associated risk of hospitalization based on more than 5 million cases reported to the Brazilian Ministry of Health from 2000-2014. METHODS: We performed a retrospective analysis of ∼5,450,000 dengue cases, relating clinical manifestations and the risk of hospitalization to age, gender, previous infection by dengue, dengue virus serotype, years of formal education, delay to first attendance and the occurrence of dengue during outbreaks and in different Brazilian regions. RESULTS: Complicated forms of dengue occurred more frequently among those younger than 10 years (3.12% vs 1.92%) and those with dengue virus 2 infection (7.65% vs 2.42%), with a delay to first attendance >;2 days (3.18% vs 0.82%) and with ≤4 years of formal education (2.02% vs 1.46%). The risk of hospitalization was higher among those aged 6-10 years old (OR 4.57; 95% CI 1.43-29.96) and those who were infected by dengue virus 2 (OR 6.36; 95% CI 2.52-16.06), who lived in the Northeast region (OR 1.38; 95% CI 1.11-2.10) and who delayed first attendance by >;5 days (composite OR 3.15; 95% CI 1.33-8.9). CONCLUSIONS: In Brazil, the occurrence of severe dengue and related hospitalization is associated with being younger than 10 years old, being infected by dengue virus 2 or 3, living in the Northeast region (the poorest and the second most populated) and delaying first attendance for more than 2 days

Effects of a Recurrent Selection Scheme, Applied to an Interspecific Hybrid \u3cem\u3ePennisetum Purpureum\u3c/em\u3e Schum. (Elephantgrass) \u3cem\u3ex Pennisetum Glaucum\u3c/em\u3e (L.) R. Br. Stuntz (Pearl Millet), on Several Seed Quality Parameters

Elephantgrass cultivars and introductions show practically no viable pure seeds and their uses in cultivated grasslands are exclusively dependent on vegetative propagation. Therefore, in large areas, sowing operation costs make unfeasible setting up new pastures. On the other hand, pearl millet is a high seed producer species though presenting some forage constraints (poor forage production, low regrowth potential after cutting or grazing and low field persistence). Recently, an hexaploid interespecific hybrid between the two species was developed (Schank & Diz, 1996), which is able to produce viable pure seeds, in variable amounts according to the genotype considered (Diz & Schank, 1995). This research aimed to check several seed quality parameters in two selected populations, derived from the original F2 interespecific hybrid population