Isolation and identification of a male-produced aggregation-sex pheromone for the velvet longhorned beetle, Trichoferus campestris.

The velvet longhorned beetle, Trichoferus campestris (Faldermann) ("VLB"; Coleoptera: Cerambycidae), is native to eastern Asia where it infests and damages a wide range of deciduous and coniferous tree species, including orchard and timber species. Immature stages of VLB are transported to new countries via international commerce, and populations have established outside the native range of the species. Here, we show that identification of pheromones of invasive pest species can be expedited by knowledge of the semiochemistry of related taxa. Histological sectioning revealed subcuticular, male-specific prothoracic glands connected to pits in the cuticle, which, in related species, are diagnostic for production of male-produced aggregation-sex pheromones, usually characterized by 2,3-alkanediol/hydroxyketone structural motifs. However, in preliminary field bioassays, beetles were not attracted by any known cerambycid pheromones. Subsequently, we identified a novel variant of the hydroxyketone motif ("trichoferone") from headspace volatiles of males. In field bioassays, synthetic trichoferone was more attractive to both sexes of VLB than previously developed high-release-rate ethanol lures, and attraction was strongly female biased. This study demonstrated the utility of the prothoracic gland trait for predicting pheromone use in cerambycid species in the subfamily Cerambycinae, and that identification of pheromones of novel species can be expedited by knowledge of pheromones of related species. Trichoferone should prove to be a valuable tool for detection of VLB in regions where the beetle is or may become established

Developing Legacy System Migration Methods and Tools for Technology Transfer

This paper presents the research results of an ongoing technology transfer project carried out in coopera- tion between the University of Salerno and a small software company. The project is aimed at developing and transferring migration technology to the industrial partner. The partner should be enabled to migrate monolithic multi-user COBOL legacy systems to a multi-tier Web-based architecture. The assessment of the legacy systems of the partner company revealed that these systems had a very low level of decompos- ability with spaghetti-like code and embedded control flow and database accesses within the user interface descriptions. For this reason, it was decided to adopt an incremental migration strategy based on the reengineering of the user interface using Web technology, on the transformation of interactive legacy programs into batch programs, and the wrapping of the legacy programs. A middleware framework links the new Web-based user interface with the Wrapped Legacy System. An Eclipse plug-in, named MELIS (migration environment for legacy information systems), was also developed to support the migration process. Both the migration strategy and the tool have been applied to two essential subsystems of the most business critical legacy system of the partner company

Identifying Cloned Navigational Patterns in Web Applications

Web Applications are subject to continuous and rapid evolution. Often programmers indiscriminately duplicate Web pages without considering systematic development and maintenance methods. This practice creates code clones that make Web Applications hard to maintain and reuse. We present an approach to identify duplicated functionalities in Web Applications through cloned navigational pattern analysis. Cloned patterns can be generalized in a reengineering process, thus to simplify the structure and future maintenance of the Web Applications. The proposed method first identifies pairs of cloned pages by analyzing similarity at structure, content, and scripting code. Two pages are considered clones if their similarity is greater than a given threshold. Cloned pages are then grouped into clusters and the links connecting pages of two clusters are grouped too. An interconnection metric has been defined on the links between two clusters to express the effort required to reengineer them as well as to select the patterns of interest. To further reduce the comprehension effort, we filter out links and nodes of the clustered navigational schema that do not contribute to the identification of cloned navigational patterns. A tool supporting the proposed approach has been developed and validated in a case study

Colostrum from cows immunized with a veterinary vaccine against bovine rotavirus displays enhanced in vitro anti-human rotavirus activity

Human rotaviruses represent a major cause of severe diarrheal disease in infants and young children. The limited impact of oral vaccines on global estimates of rotavirus mortality and the suboptimal use of oral rehydration justify the need for alternative prophylactic and therapeutic strategies, especially for immunocompromised hosts. The protective effects of colostrum\u2014the first milk produced during the initial 24 to 48 h after parturition\u2014are well documented in the literature. In particular, the ingestion of hyperimmune bovine colostrum has been proposed as an alternative preventive approach against human rotavirus gastroenteritis. Although the immunization of pregnant cows with human rotavirus boosts the release of specific immunoglobulin G in bovine colostrum, it raises regulatory and safety issues. In this study, we demonstrated that the conventional bovine rotavirus vaccine is sufficient to enhance the anti-human rotavirus protective efficacy of bovine colostrum, thus providing a conservative approach to produce hyperimmune bovine colostrum, making it exploitable as a functional food

The Reed-Stanton press rig for the generation of reproducible fingermarks : towards a standardised methodology for fingermark research

In the search for better or new methods/techniques to visualise fingermarks or to analyse them exploiting their chemical content, fingermarks inter-variability may hinder the assessment of the method effectiveness. Variability is due to changes in the chemical composition of the fingermarks between different donors and within the same donor, as well as to differential contact time, pressure and angle. When validating a method or comparing it with existing ones, it is not always possible to account for this type of variability. One way to compensate for these issues is to employ, in the early stages of the method development, a device generating reproducible fingermarks. Here the authors present their take on such device, as well as quantitatively describing its performance and benefits against the manual production of marks. Finally a short application is illustrated for the use of this device, at the method developmental stages, in an emerging area of fingerprinting research concerning the retrieval of chemical intelligence from fingermarks

Cooking influence on physico-chemical fruit characteristics of eggplant (Solanum melongena L.)

Physico-chemical traits of three eggplant genotypes ("Tunisina", "Buia" and "L 305") were evaluated before and after two cooking treatments (grilling and boiling). Different genotypes revealed different changes after cooking, with "Tunisina" showing a better retention of phytochemicals with respect to other two genotypes. The main physical phenomena were water loss during grilling, and dry matter loss after boiling. Chlorogenic acid, the main phenolic in eggplant, resulted higher in grilled samples, while delphinidin glycosides resulted more retained in boiled samples. Glycoalkaloids, thiols and biogenic amines were generally stable, while 5-hydroxy-methyl-furfural was found only in grilled samples. Interestingly, Folin-Ciocalteu index and free radical scavenging capacity, measured with three different assays, were generally increased after cooking, with a greater formation of antioxidant substances in grilled samples. NMR relaxation experiments clarified the hypothesis about the changes of eggplant compounds in terms of decomposition of larger molecules and production of small ones after cooking

A multi-parameter field monitoring system to investigate the dynamics of large earth slides–earth flows in the Northern Apennines, Italy

Large earth slides and rocks lides evolving into earth flows are quite widespread in the Northern Italian Apennines. Despite being simply referred to as landslides, many of them are, in fact, large complexes of landslides. They evolved through multiple and/or successive movements, undergoing partial and/or total reactivations. The reactivation of pre-existing landslide bodies is the prevalent mechanism for the known landslide events, as the historical records and the technical reports indicate. Landslide reactivation is, indeed, a relevant topic from the perspective of risk assessment and mitigation. A multi-parameter monitoring system was installed on a large complex of landslides that underwent partial or total reactivations after heavy rainfall events, causing damages to buildings and infrastructures. Two clusters of automatic piezometers—each coupled with an inclinometer—and a time-lapse resistivity deployment were the core of the monitoring system. A weather station, collecting data from subsurface thermometers, and a water content probe completed the system. After the construction of a new geological model of the slope, this study aimed at understanding the possible mechanisms leading to the reactivation of the landslide. This goal was achieved by gaining insights into the process of rainfall infiltration into the landslide deposits, by determining the groundwater flow and evaluating the landslide displacements. The monitoring system captured the processes that took place in the landslide bodies and the bedrock in response to a rainfall event in early February 2017, which followed a dry period of eight months. The recorded data provided indications on the variation of the hydraulic head in the groundwater within the landslide and the bedrock, particularly at the sliding surfaces. The electrical conductivity of the groundwater and the resistivity of the terrain varied across the failure surfaces. In particular, a sudden increase in the electrical conductivity was related to the locations of the main sliding surfaces. The joint analysis of time-lapse resistivity, hydraulic heads, and groundwater electrical conductivity helped identify the locations of weaker levels within the landslide masses, which were confirmed by data from inclinometers. This study improved the knowledge of the hydrogeological behaviour of a complex of landslides in heterogeneous low-permeability media. Moreover, the obtained results contributed to the understanding of the role played by different portions of the landslide complex in the evolution of the movement

Hydrodynamics in evaporate-bearing fine-grained successions investigated through an interdisciplinary approach : A test study in southern Italy-hydrogeological behaviour of heterogeneous low-permeability media

Messinian evaporates are widely distributed in the Mediterranean Sea as outcropping sediments in small marginal basins and in marine cores. Progressive filling of subbasins led to the formation of complex aquifer systems in different regions where hypersaline and fresh water coexist and interact in different manner. It also generates a significant diversification of groundwater hydrochemical signature and different microbial communities. In the case study, the hydrogeology and hydrochemistry of the whole system are influenced by good hydraulic connection between the shallower pyroclastic horizon and the underlying evaporate-bearing fine-grained Messinian succession. This is demonstrated by the merge of hydrogeological, chemical, isotopic, and microbiological data. No mixing with deep ascending waters has been observed. As shown by geophysical, hydraulic, and microbiological investigations, the hydraulic heterogeneity of the Messinian bedrock, mainly due to karstified evaporitic interstrata/lenses, causes the hydraulic head to significantly vary with depth. Somewhere, the head increases with the depth's increase and artesian flow conditions are locally observed. Moreover, the metagenomic investigations demonstrated the existence of a poor hydraulic connection within the evaporate-bearing fine-grained succession at metric and decametric scales, therefore leading to a patchwork of geochemical (and microbiological) subenvironments