The Scleractinian genus Actinacis. Systematic revision and stratigraphic record of the Tertiary species with special regard to Italian occurrences.

Twenty-seven Tertiary species belonging to the cosmopolitan reef-building scleractinian genus Actinacis, have been described in the literature. A detailed systematic revision has been carried out for Italian and European species by thin section measurements. A combination of several measured corallite morphologic characters and diagnostic features is proposed as a realiable tool for species recognition. Preliminary examination of the stratigraphic and geographic distribution of Actinacis species suggests that: 1) the Tertiary distribution of the genus ranges from the Late Paleocene to the Late Oligocene; 2) the highest species diversity occrred during the Middle Eocene, when the genus consisted of a relatively large number of geographically restricted species; 3) only two widespread species survived the Eocene/Oligocene turnover and reached the Late Oligocene, when the genus became globally extinct

THE INHIBITORY EFFECT OF PROPOLIS AND CAFFEIC ACID PHENETHYLESTER ON CYCLOOXYGENASE ACTIVITY IN J774 MACROPHAGES.

The effect of an ethanolic extract of propolis, with and without CAPE, and some of its components on cyclooxygenase (COX-1 and COX-2) activity in J774 macrophages has been investigated. COX-1 and COX-2 activity, measaured as prostaglandin E-2 (PGE(2)) production, were concentration-dependently inhibited by propolis (C x 10(-3)-3 x 10(2) mugml(-1)) with an IC50 of 2.7 mugml(-1) and 4.8 x 10(-2) mugml(-1), respectively. Among the compounds tested pinocembrin and caffeic, ferulic, cinnamic and chlorogenic acids did not affect the activity of COX isoforms. Conversely, CAPE (2.8 x 10(-4)-28 mugml(-1); 10(-9)-10(-4) M) and galangin (2.7 x 10(-4)-27 mugml(-1); 10(-9)-10(-4) M) were effective, the last being about ten-twenty times less potent. In fact the IC50 of CAPE for COX-1 and COX-2 were 4.4 x 10(-1) mugml(-1) (1.5 x 10(-6) M) and 2 x 10(-3) mugml(-1) (6.3 x 10(-9) M), respectively. The IC50 of galangin were 3.7 mugml(-1) (15 x 10(-6) M) and 3 x 10(-2) mugml(-1) (120 x 10(-1) M), for COX-1 and COX-2 respectively. To better investigate the role of CAPE, we tested the action of the ethanolic extract of propolis deprived of CAPE, which resulted about ten times less potent than the extract with CAPE in the inhibition of both COX-1 and COX-2, with an IC50 of 30 mugml(-1) and 5.3 x 10(-1) mugml(-1), respectively. Moreover the comparison of the inhibition curves showed a significant difference (p < 0.001). These results suggest that both CAPE and galangin contribute to the overall activity of propolis, CAPE being more effective

THE SCLERACTINIAN GENUS ACTINACIS . SYSTEMATIC REVISION AND STRATIGRAPHIC RECORD OF THE TERTIARY SPECIES WITH SPECIAL REGARD TO ITALIAN OCCURRENCES

Twenty-seven Tertiary species belonging to the cosmopolitan reef-building scleractinian genus Actinacis, have been described in the literature. A detailed systematic revision has been  carried out for Italian and European species by thin section  measurements of material mostly sampled from several Italian localities by observation of some holotypes and topotypes and accurare examination of the available literature. A combination of several measured corallite morphologic characters and diagnostic features is proposed as a reliable tool for species recognition. Results indicate that only three species, of the twelve described for the Italian Tertiary sites, actually belong to the genus Actinacis. Moreover, a list of synonyms and a stratigraphic range chart of the established species are proposed. An approximate taxonomic identification and stratigraphic distribution are given for those species not included in the systematic revision. Preliminary examination of the stratigraphic and geographic distribution of Actinacis species suggests that:1) the Tertiary distribution of the genus ranges from the Late Paleocene to the Late Oligocene (from Late Cuisian to Middle Chattian as concerns ltaly); 2)the highest species diversity occurred during the Middle Eocene, when the genus consisted of a relatively large number of geographically restricted species; 3) only two widespread species survived the Eocene/Oligocene turnover and reached the Late Oligocene, when the genus became globally extinct

Validation Procedure for Predictive Functions of Driver Behaviour on Two-Lane Rural Roads

The study presented here aims to validate some operating speed prediction models calibrated on two-lane rural roads by using speed data collected in Northern and Southern Italy. Operating speed is defined as the speed at which drivers of passenger cars travel on a dry road in free flow conditions during daylight hours and it is calculated using a specific percentile of speed distribution, typically the 85th. Speed measurements were carried out by using laser detectors in connection with previous environmental and traffic conditions. The study is addressed to emphasize the reliability and easy application of one predictive speed model working both on tangent segments and on circular curves. The calibration phase involved roads in the Northern Italy, while the validation phase involved roads in the Southern Italy. Three models were validated applying them on eight two-lane rural roads falling within the road network of the Province of Salerno with features that reflect those adopted in the calibration phase; the selected models to be validated present the simplest analytical structure for type and number of explanatory variables and for the performance diagram shape of the operating speed values. The validation procedure was to estimate some synthetic statistical parameters as mean absolute deviation, mean squared error and coefficient of variation. The results allow in a simple way to trace continuous operating speed profiles on two-lane rural roads and to carry out safety analyses on the horizontal alignment

Heparanase and macrophage interplay in the onset of liver fibrosis

Abstract The heparan sulfate endoglycosidase heparanase (HPSE) is involved in tumor growth, chronic inflammation and fibrosis. Since a role for HPSE in chronic liver disease has not been demonstrated to date, the current study was aimed at investigating the involvement of HPSE in the pathogenesis of chronic liver injury. Herein, we revealed that HPSE expression increased in mouse livers after carbon tetrachloride (CCl4)-mediated chronic induction of fibrosis, but with a trend to decline during progression of the disease. In mouse fibrotic liver tissues HPSE immunostaining was restricted in necro-inflammatory areas, co-localizing with F4/80 macrophage marker and TNF-α. TNF-α treatment induced HPSE expression as well as HPSE secretion in U937 macrophages. Moreover, macrophage-secreted HPSE regulated the expression of α-SMA and fibronectin in hepatic stellate LX-2 cells. Finally, HPSE activity increased in the plasma of patients with liver fibrosis but it inversely correlated with liver stiffness. Our results suggest the involvement of HPSE in early phases of reaction to liver damage and inflammatory macrophages as an important source of HPSE. HPSE seems to play a key role in the macrophage-mediated activation of hepatic stellate cells (HSCs), thus suggesting that HPSE targeting could be a new therapeutic option in the treatment of liver fibrosis

Molecular basis, diagnosis and clinical management of mucopolysaccharidoses

Mucopolysaccharidoses (MPSs) are a group of hereditary, monogenic disorders caused by lysosomal storage of glycosaminoglycans. Their incidence as a group is between 1:25,000 and 1:45,000. At present 11 different enzyme deficiencies are know to be responsible of 7 similar but distinct diseases. The diagnosis is suspected clinically but must be confirmed through biochemical, enzymatic and molecular analysis. Prenatal diagnosis is feasible for each disease. The phenotype worsens with age, due to progressive storage, and mainly involves mucosal tissue, upper airways and lungs, bones and joints, central and peripheral nervous system, heart, liver, eye and ear. Any type of MPSs, is characterized by a wide variability of phenotype ranging from a severe fetal-neonatal disease to an attenuated form diagnosed in adult individuals. Recently new treatments, like hematopoietic stem cell transplantation and enzymatic replacement therapy, became available for many of these disorders entailing the urgency of early diagnosis to allow access to therapies. Thanks to therapies these patients have a longer life than in the past and this implies that also palliative treatments, of which the cardiological ones have a prominent part, must be undertaken diligently. The cardiologist may face, more frequently than expected, with the need to diagnose a patient with MPS who was not recognized by other specialists. The echocardiographic features of these patients are typical and may help in the clinical diagnosis. The future probably deserves to these disorders other new treatments or combination therapies, which might further improve prognosis of these diseases

Experimental Analysis of Failure Mechanisms in Masonry-PFRP Profiles Connections

Fibre-reinforced polymer (FRP) profiles, with their low density, high durability, and ease of construction, are particularly suitable for the retrofit of traditional masonry structures, particularly historic constructions in seismic zones. However, a critical aspect of this new technology application is the connection between FRP profiles and masonry walls. So far, no research studies are available on this subject. The authors carried out a preliminary experimental campaign on different connection systems between masonry and pultruded glass-fibre-reinforced polymer (GFRP) profiles. The note presents the immediate results of this study, focusing on the performance and collapse mechanisms; the study may contribute to the development of an effective connection system between masonry and FRP profiles to be adopted in the retrofitting of existing building with juxtaposed FRP frames

Invasive meningococcal disease in the Veneto region of Italy: A capture-recapture analysis for assessing the effectiveness of an integrated surveillance system

open8noBACKGROUND: Epidemiology of Neisseria meningitidis has been changing since the introduction of universal vaccination programmes against meningococcal serogroup C (MenC) and meningococcal serogroup B (MenB) has now become dominant. This study aimed to analyse the cases reported in institutional data recording systems to estimate the burden of invasive meningococcal diseases (IMDs) and assess the effectiveness of surveillance in Veneto region (Italy). METHODS: Analysis was performed from 2007 to 2014 on data recorded in different systems: Mandatory Notification System, National Surveillance of Invasive Bacterial Diseases System and Laboratories Surveillance System (LSS), which were pooled into a combined surveillance system (CSS) and hospital discharge records (HDRs). A capture-recapture method was used and completeness of each source estimated. Number of cases with IMD by source of information and year, incidence of IMD by age group, case fatality rate (CFR) and distribution of meningococcal serogroups by year were also analysed. RESULTS: Combining the four data systems enabled the identification of 179 confirmed cases with IMD, achieving an overall sensitivity of 94.7% (95% CI: 90.8% to 98.8%), while it was 76.7% (95% CI: 73.6% to 80.1%) for CSS and 77.2% (95% CI: 74.1% to 80.6%) for HDRs. Typing of isolates was done in 80% of cases, and 95.2% of the typed cases were provided by LSS. Serogroup B was confirmed in 50.3% of cases. The estimated IMD notification rate (cases with IMD diagnosed and reported to the surveillance systems) was 0.48/100 000 population, and incidence peaked at 6.2/100 000 in children aged <1 year old (60.9% due to MenB), and increased slightly in the age group between 15 and 19 years (1.1/100 000). A CFR of 14% was recorded (8.7% in paediatric age). CONCLUSIONS: Quality of surveillance systems relies on case ascertainment based on serological characterisation of the circulating strains by microbiology laboratories. All available sources should be routinely combined to improve the epidemiology of IMD and the information used by public health departments to conduct timely preventive measures.openBaldovin, Tatjana; Lazzari, Roberta; Cocchio, Silvia; Furlan, Patrizia; Bertoncello, Chiara; Saia, Mario; Russo, Francesca; Baldo, VincenzoBaldovin, Tatjana; Lazzari, Roberta; Cocchio, Silvia; Furlan, Patrizia; Bertoncello, Chiara; Saia, Mario; Russo, Francesca; Baldo, Vincenz

Analytical Assessment of Effective Maintenance Operations on At-Grade Unsignalized Intersections

This chapter describes a methodological structure to support and improve the decision-making process for redesigning the geometric configurations of substandard sites and thus reduce crash risk factors on at-grade three-leg and four-leg intersections with stop-control on minor roads and single-lane roundabouts belonging to a two-lane rural road network located in Southern Italy. Starting from an initial evaluation of the risk level at each investigated site and adopting a procedure developed by the Italian National Research Council based on an estimated crash rate level, a more precise hierarchy of intersections with “black” rankings was developed. In addition, new geometric configurations for the most hazardous sites were suggested based on a statistical comparison in terms of safety and Level of Service (LoS). The effectiveness of the strategies was validated by computing the expected LoS and safety by adopting an empirical Bayesian analysis and performance functions centered on a revised Highway Safety Manual procedure reflecting the context of the study

OPERATING SPEED AS A KEY FACTOR IN STUDYING THE DRIVER BEHAVIOUR IN A RURAL CONTEXT

The research aims to explore the effects of geometric road features on driver speed behaviour in order to identify unsafe road segments where high reductions in speed between successive road elements occur. The sample involves two-lane rural roads on flat terrain (vertical grade less than 5%) in Southern Italy, totalling 184 km without spiral transition curves between the tangent segments and circular elements. The testing was carried out on 567 study sites, of which 248 are on circular curves and 319 on tangents. Speed data collection was carried out in environmental and traffic conditions using a laser. The conditions were the following: dry roads, free flow conditions, daylight hours and good weather conditions. The main goal was to calibrate and validate different operating speed prediction models: a) one model on tangent segments; b) one model on circular curves; c) only one model to be used at the same time on tangents and circular curves. The validation process involved almost 10% of the total road network length, that was removed from the calibration phase. The speed measurements of each of the first two datasets (a, b) were grouped into ten homogeneous substrates while for the remaining dataset (c) sixteen substrates were defined by using a hard c-means algorithm. Two statistical criteria were used to remove anomalous operating speed values from each group of three datasets, namely, the Chauvenet criterion and the Vivatrat method. The first criterion was preferred in the final process of model selection. The results of the first filtering procedure showed more homogeneous samples that guaranteed a higher correlation coefficient and lower residuals of the predictive models during the validation phase than the Vivatrat method. The models were developed using an Ordinary Least Squares (OLS) method. The explanatory variables were total segment length, lane width, curvature of the road element, the curvature change rate on homogeneous road segments, and the number of residential driveways per km. ANOVA and additional synthetic statistical parameters were assessed to check the effectiveness of using a single general model to predict operating speeds at the same time on tangents and on circular curves alike. The results suggested the reliability of this hypothesis and its effectiveness in bringing advantages during the application phase