Orla. Experimentações em narrativa sequencial

ORLA é uma exposição e uma publicação online que resultam de alguns trabalhos propostos pelos alunos, em consequência de exercícios e reflexões realizados no contexto da UC Ilustração e Narrativa Visual, do 1 ° semestre do 1 ° ano da Licenciatura em Artes Plásticas.Uma orla, como uma cercadura envolvendo explorações realizadas num contexto de procura, momentos de descoberta de possibilidades e caminhos próprios, inserindo-se como um pequeno passo (entre tantos outros) a favor de processos de reflexão, de criação de direções próprias de cada aluno. O que é criado, como é criado e o que daí é visualizado. Entre visões e processos ligados à comunicação e/ou à exploração direcionada. Reflexões e experimentações em ilustração e narrativa sequencial com diferentes grafismos e materialidades. a favor de narrativa e/ou poética em objetos. Relacionar imagens e sub-imagens, ilustrações e vinhetas, pistas e direções. Relacionar o que acontece na vinheta e o que acontece fora da vinheta, o que surge no enquadramento, o todo , e os elementos que o compõem. Criar foco. Tentativas e erros como parte de percursos que estão no seu início.info:eu-repo/semantics/publishedVersio

Pretreatment on anaerobic sludge for enhancement of biohydrogen production from cassava processing wastewater

Methods for the enrichment of an anaerobic sludge with H2-producing bacteria have been compared by using cassava processing wastewater as substrate.The sludge was submitted to three different pretreatments: 1) heat pretreatment by boiling at 98 °C for 15 min., 2) heat pretreatment followed by sludge washout in a Continuous Stirring Tank Reactor (CSTR) operated at a dilution rate (D) of 0.021 h-1, and 3) sludge washout as the sole enrichment method. The pretreated sludge and the sludge without pretreatment (control) were employed in the seeding of 4 batch bioreactors, in order to verify the volume and composition of the generated biogas. Maximum H2 production rates (Rm) from the pretreated sludges, were estimated by the modified Gompertz model. Compared to the control, H2 production was ca. 4 times higher for the sludge submitted to the heat pretreatment only and for the sludge subjected to heat pretreatment combined with washout, and 10 times higher for washout. These findings demonstrated that the use of sludge washout as the sole sludge pretreatment method was the most effective in terms of H2 production, as compared to the heat and to the combined heat and washout pretreatments.Methods for the enrichment of an anaerobic sludge with H2-producing bacteria have been compared by using cassava processing wastewater as substrate. The sludge was submitted to three different pretreatments: 1) heat pretreatment by boiling at 98°C for 15 min., 2) heat pretreatment followed by sludge washout in a Continuous Stirring Tank Reactor (CSTR) operated at a dilution rate (D) of 0.021 h-1, and 3) sludge washout as the sole enrichment method. The pretreated sludge and the sludge without pretreatment (control) were employed in the seeding of 4 batch bioreactors, in order to verify the volume and composition of the generated biogas. Maximum H2 production rates (Rm) from the pretreated sludges were estimated by the modified Gompertz model. Compared to the control, H2 production was ca. 4 times higher for the sludge submitted to the heat pretreatment only and for the sludge subjected to heat pretreatment combined with washout, and 10 times higher for washout. These findings demonstrated that the use of sludge washout as the sole sludge pretreatment method was the most effective in terms of H2 production, as compared to the heat and to the combined heat and washout pretreatments

Antón Lizardo (Veracruz) (Tenedero). Cartas náuticas. 1818

En el ángulo inferior izquierdo incluye: "Prevención para dirigirse a este Fondeadero. Tiene este p.r excelencia cuatro entradas de mucho fondo, por donde se pueden dirigir buques de todos calados. Las preferibles son las que forman los bajos en la costa; y como naturalmente será siempre la del Oeste, la que se elija cuando se halle a una regular distancia de la Ysla Blanquita se gobernará al Este corregido, cuyo Rumbo se continuará hasta estar un poco internado, que se enmendará más al Norte para fondear donde se quiera". En el ángulo inferior derecho incluye: "Nota. Los números de la sonda son brazas de 6 pies de Burgos. La A. significa arena. P. piedra. N. negra. L. lama. Cº. cascajo. Cª. conchuela. G. gruesa. Y. yerba. Fº. fango. B. blanca. Bº. barroso. Situación de Goleta Belona. Variación observada en Salmedina 8º NE. México 16 de agosto de 1818. Valentín de Ampudia" (rúbrica). Al verso anotado a lápiz: "Nº 33"Escala hallada del valor de tres millas geográficas españolas de 60 al gradoSumario: Presenta un plano detallado del Tenedero de Antón Lizardo desde la Boca del río de Medellín hasta la punta del Colloll y Salado Chico. Señala la Laxa del Giote en la punta de Antón Lizardo, la isla Blanquita, la isla de Salmedina con sus arrecifes, el arrecife del Palo, la isla y arrecife del Medio y El RizoCopia Digital. Real Academia de la Historia : 2010Donado a la RAH por su correspondiente Fernando de Gabriel y Ruiz de Apodaca (Memorias de la RAH, t. X, 1886, nº 33, p. 845)Orientado con castillo en flecha. Relieve por sombreado. Sondas batimétricas. Fondeaderos. ArrecifesEs copia reducida del original conservado en la misma colección (Manso Porto, Carmen, Cartografía histórica de América, nº 92. Sign. C -I a 20 p). Manuscrito levantado por orden del Rey y remitido al secretario de Estado y del Despacho Universal de Marina José Vázquez Figueroa en 1818, por Francisco Murías, comandante del Apostadero de Veracruz; copiado por orden del virrey Juan Ruiz de Apodaca y reducido por Rafael Mª Calvo, capitán del regimiento de Infantería; firmado y rubricado por Valentín de Ampudia en "México, 16 de agosto de 1818"Manuscrito dibujado a plumilla en tinta chinaEn la parte superior central, entre el título, inserta escudo con castillo; al fondo un navío cerca de la cost

Blood DNA methylation marks discriminate Chagas cardiomyopathy disease clinical forms

Chagas disease is a parasitic disease from South America, affecting around 7 million people worldwide. Decades after the infection, 30% of people develop chronic forms, including Chronic Chagas Cardiomyopathy (CCC), for which no treatment exists. Two stages characterized this form: the moderate form, characterized by a heart ejection fraction (EF) ≥ 0.4, and the severe form, associated to an EF < 0.4. We propose two sets of DNA methylation biomarkers which can predict in blood CCC occurrence, and CCC stage. This analysis, based on machine learning algorithms, makes predictions with more than 95% accuracy in a test cohort. Beyond their predictive capacity, these CpGs are located near genes involved in the immune response, the nervous system, ion transport or ATP synthesis, pathways known to be deregulated in CCCs. Among these genes, some are also differentially expressed in heart tissues. Interestingly, the CpGs of interest are tagged to genes mainly involved in nervous and ionic processes. Given the close link between methylation and gene expression, these lists of CpGs promise to be not only good biomarkers, but also good indicators of key elements in the development of this pathology

Epigenetic regulation of transcription factor binding motifs promotes Th1 response in Chagas disease cardiomyopathy

International audienceChagas disease, caused by the protozoan Trypanosoma cruzi , is an endemic parasitic disease of Latin America, affecting 7 million people. Although most patients are asymptomatic, 30% develop complications, including the often-fatal Chronic Chagasic Cardiomyopathy (CCC). Although previous studies have demonstrated some genetic deregulations associated with CCCs, the causes of their deregulations remain poorly described. Based on bulk RNA-seq and whole genome DNA methylation data, we investigated the genetic and epigenetic deregulations present in the moderate and severe stages of CCC. Analysis of heart tissue gene expression profile allowed us to identify 1407 differentially expressed transcripts (DEGs) specific from CCC patients. A tissue DNA methylation analysis done on the same tissue has permitted the identification of 92 regulatory Differentially Methylated Regions (DMR) localized in the promoter of DEGs. An in-depth study of the transcription factors binding sites (TFBS) in the DMRs corroborated the importance of TFBS’s DNA methylation for gene expression in CCC myocardium. TBX21, RUNX3 and EBF1 are the transcription factors whose binding motif appears to be affected by DNA methylation in the largest number of genes. By combining both transcriptomic and methylomic analysis on heart tissue, and methylomic analysis on blood, 4 biological processes affected by severe CCC have been identified, including immune response, ion transport, cardiac muscle processes and nervous system. An additional study on blood methylation of moderate CCC samples put forward the importance of ion transport and nervous system in the development of the disease

Table_1_Blood DNA methylation marks discriminate Chagas cardiomyopathy disease clinical forms.docx

Chagas disease is a parasitic disease from South America, affecting around 7 million people worldwide. Decades after the infection, 30% of people develop chronic forms, including Chronic Chagas Cardiomyopathy (CCC), for which no treatment exists. Two stages characterized this form: the moderate form, characterized by a heart ejection fraction (EF) ≥ 0.4, and the severe form, associated to an EF < 0.4. We propose two sets of DNA methylation biomarkers which can predict in blood CCC occurrence, and CCC stage. This analysis, based on machine learning algorithms, makes predictions with more than 95% accuracy in a test cohort. Beyond their predictive capacity, these CpGs are located near genes involved in the immune response, the nervous system, ion transport or ATP synthesis, pathways known to be deregulated in CCCs. Among these genes, some are also differentially expressed in heart tissues. Interestingly, the CpGs of interest are tagged to genes mainly involved in nervous and ionic processes. Given the close link between methylation and gene expression, these lists of CpGs promise to be not only good biomarkers, but also good indicators of key elements in the development of this pathology.</p

Table_2_Blood DNA methylation marks discriminate Chagas cardiomyopathy disease clinical forms.docx

Chagas disease is a parasitic disease from South America, affecting around 7 million people worldwide. Decades after the infection, 30% of people develop chronic forms, including Chronic Chagas Cardiomyopathy (CCC), for which no treatment exists. Two stages characterized this form: the moderate form, characterized by a heart ejection fraction (EF) ≥ 0.4, and the severe form, associated to an EF < 0.4. We propose two sets of DNA methylation biomarkers which can predict in blood CCC occurrence, and CCC stage. This analysis, based on machine learning algorithms, makes predictions with more than 95% accuracy in a test cohort. Beyond their predictive capacity, these CpGs are located near genes involved in the immune response, the nervous system, ion transport or ATP synthesis, pathways known to be deregulated in CCCs. Among these genes, some are also differentially expressed in heart tissues. Interestingly, the CpGs of interest are tagged to genes mainly involved in nervous and ionic processes. Given the close link between methylation and gene expression, these lists of CpGs promise to be not only good biomarkers, but also good indicators of key elements in the development of this pathology.</p

Table_3_Blood DNA methylation marks discriminate Chagas cardiomyopathy disease clinical forms.docx

Chagas disease is a parasitic disease from South America, affecting around 7 million people worldwide. Decades after the infection, 30% of people develop chronic forms, including Chronic Chagas Cardiomyopathy (CCC), for which no treatment exists. Two stages characterized this form: the moderate form, characterized by a heart ejection fraction (EF) ≥ 0.4, and the severe form, associated to an EF < 0.4. We propose two sets of DNA methylation biomarkers which can predict in blood CCC occurrence, and CCC stage. This analysis, based on machine learning algorithms, makes predictions with more than 95% accuracy in a test cohort. Beyond their predictive capacity, these CpGs are located near genes involved in the immune response, the nervous system, ion transport or ATP synthesis, pathways known to be deregulated in CCCs. Among these genes, some are also differentially expressed in heart tissues. Interestingly, the CpGs of interest are tagged to genes mainly involved in nervous and ionic processes. Given the close link between methylation and gene expression, these lists of CpGs promise to be not only good biomarkers, but also good indicators of key elements in the development of this pathology.</p

Management of coronary disease in patients with advanced kidney disease

BACKGROUND Clinical trials that have assessed the effect of revascularization in patients with stable coronary disease have routinely excluded those with advanced chronic kidney disease. METHODS We randomly assigned 777 patients with advanced kidney disease and moderate or severe ischemia on stress testing to be treated with an initial invasive strategy consisting of coronary angiography and revascularization (if appropriate) added to medical therapy or an initial conservative strategy consisting of medical therapy alone and angiography reserved for those in whom medical therapy had failed. The primary outcome was a composite of death or nonfatal myocardial infarction. A key secondary outcome was a composite of death, nonfatal myocardial infarction, or hospitalization for unstable angina, heart failure, or resuscitated cardiac arrest. RESULTS At a median follow-up of 2.2 years, a primary outcome event had occurred in 123 patients in the invasive-strategy group and in 129 patients in the conservative-strategy group (estimated 3-year event rate, 36.4% vs. 36.7%; adjusted hazard ratio, 1.01; 95% confidence interval [CI], 0.79 to 1.29; P=0.95). Results for the key secondary outcome were similar (38.5% vs. 39.7%; hazard ratio, 1.01; 95% CI, 0.79 to 1.29). The invasive strategy was associated with a higher incidence of stroke than the conservative strategy (hazard ratio, 3.76; 95% CI, 1.52 to 9.32; P=0.004) and with a higher incidence of death or initiation of dialysis (hazard ratio, 1.48; 95% CI, 1.04 to 2.11; P=0.03). CONCLUSIONS Among patients with stable coronary disease, advanced chronic kidney disease, and moderate or severe ischemia, we did not find evidence that an initial invasive strategy, as compared with an initial conservative strategy, reduced the risk of death or nonfatal myocardial infarction