Thin Layer Composite Unimorph Ferroelectric Driver and Sensor

A method for forming ferroelectric wafers is provided. A prestress layer is placed on the desired mold. A ferroelectric wafer is placed on top of the prestress layer. The layers are heated and then cooled causing the ferroelectric wafer to become prestressed. The prestress layer may include reinforcing material and the ferroelectric wafer may include electrodes or electrode layers may be placed on either side of the ferroelectric layer. Wafers produced using this method have greatly improved output motion

Role of the area postrema in three putative measures of motion sickness in the rat

After thermal cauterization of the area postrema in rats, the absence of conditioned taste aversion of sucrose paired with lithium chloride (0.15M, 3.3 ml/kg) was used as a pharmacologic/behavioral index of area postrema damage. In a subsequent experiment the effects of area postrema lesions on three measures proposed as species-relevant measures of motion sickness were studied, using off-vertical rotation at 150 deg/s for either 30 or 90 min. Lesions of area postrema did not alter postrotational suppression of drinking or amount of defecation during motion. The initial acquisition of conditioned taste aversion to a novel cider vinegar solution paired with motion was not affected by lesioning of the area postrema, but these taste aversions extinguished more slowly in lesioned rats than in sham-operates or intact controls. Results are discussed in terms of proposed humoral factors which may induce motion sickness and in light of recent data on the role of the area postrema in similar measures in species possessing the complete emetic reflex

Constraints on the Progenitor of SN 2010jl and Pre-Existing Hot Dust in its Surrounding Medium

A search for the progenitor of SN~2010jl, an unusually luminous core-collapse supernova of Type~IIn, using pre-explosion {\it Hubble}/WFPC2 and {\it Spitzer}/IRAC images of the region, yielded upper limits on the UV and near-infrared (IR) fluxes from any candidate star. These upper limits constrain the luminosity and effective temperature of the progenitor, the mass of any preexisting dust in its surrounding circumstellar medium (CSM), and dust proximity to the star. A {\it lower} limit on the CSM dust mass is required to hide a luminous progenitor from detection by {\it Hubble}. {\it Upper} limits on the CSM dust mass and constraints on its proximity to the star are set by requiring that the absorbed and reradiated IR emission not exceed the IRAC upper limits. Using the combined extinction-IR emission constraints we present viable $M_d-R_1$ combinations, where $M_d$ and $R_1$ are the CSM dust mass and its inner radius. These depend on the CSM outer radius, dust composition and grain size, and the properties of the progenitor. The results constrain the pre-supernova evolution of the progenitor, and the nature and origin of the observed post-explosion IR emission from SN~2010jl. In particular, an $\eta$~Car-type progenitor will require at least 4~mag of visual extinction to avoid detection by the {\it Hubble}. This can be achieved with dust masses $\gtrsim 10^{-3}$~\msun\ (less than the estimated 0.2-0.5~\msun\ around $\eta$~Car) which must be located at distances of $\gtrsim 10^{16}$~cm from the star to avoid detection by {\it Spitzer}.Comment: Accepted for publication in the ApJ. 14 pages 10 figures. The complete figure set for Figure 10 (24 images) is available in the online journa

The infrared echo of SN2010jl and its implications for shock breakout characteristics

SN 2010jl is a Type IIn core collapse supernova whose radiative output is powered by the interaction of the SN shock wave with its surrounding dense circumstellar medium (CSM). After day ~60, its light curve developed a NIR excess emission from dust. This excess could be a thermal IR echo from pre-existing CSM dust, or emission from newly-formed dust either in the cooling postshock region of the CSM, or in the cooling SN ejecta. Recent analysis has shown that dust formation in the CSM can commence only after day ~380, and has also ruled out newly-formed ejecta dust as the source of the NIR emission. The early (< 380 d) NIR emission can therefore only be attributed to an IR echo. The H-K color temperature of the echo is about 1250 K. The best fitting model requires the presence of about 1.6e-4 Msun of amorphous carbon dust at a distance of 2.2e16 cm from the explosion. The CSM-powered luminosity is preceded by an intense burst of hard radiation generated by the breakout of the SN shock through the stellar surface. The peak burst luminosity seen by the CSM dust is significantly reduced by Thomson scattering in the CSM, but still has the potential of evaporating the dust needed to produce the echo. We show that the survival of the echo-producing dust provides important constraints on the intensity, effective temperature, and duration of the burst.Comment: Accepted for publication in the ApJ, 20 pages, 1 table, and 17 figure

Local mapping of dissipative vortex motion

We explore, with unprecedented single vortex resolution, the dissipation and motion of vortices in a superconducting ribbon under the influence of an external alternating magnetic field. This is achieved by combing the phase sensitive character of ac-susceptibility, allowing to distinguish between the inductive-and dissipative response, with the local power of scanning Hall probe microscopy. Whereas the induced reversible screening currents contribute only inductively, the vortices do leave a fingerprint in the out-of-phase component. The observed large phase-lag demonstrates the dissipation of vortices at timescales comparable to the period of the driving force (i.e. 13 ms). These results indicate the presence of slow microscopic loss mechanisms mediated by thermally activated hopping transport of vortices between metastable states.Comment: 5 pages, 2 figure

DNAzyme-mediated recovery of small recombinant RNAs from a 5S rRNA-derived chimera expressed in Escherichia coli

Background: Manufacturing large quantities of recombinant RNAs by overexpression in a bacterial host is hampered by their instability in intracellular environment. To overcome this problem, an RNA of interest can be fused into a stable bacterial RNA for the resulting chimeric construct to accumulate in the cytoplasm to a sufficiently high level. Being supplemented with cost-effective procedures for isolation of the chimera from cells and recovery of the recombinant RNA from stabilizing scaffold, this strategy might become a viable alternative to the existing methods of chemical or enzymatic RNA synthesis. Results: Sequence encoding a 71-nucleotide recombinant RNA was inserted into a plasmid-borne deletion mutant of the Vibrio proteolyticus 5S rRNA gene in place of helix III - loop C segment of the original 5S rRNA. After transformation into Escherichia coli, the chimeric RNA (3譸en aRNA) was expressed constitutively from E. coli rrnB P1 and P2 promoters. The RNA chimera accumulated to levels that exceeded those of the host's 5S rRNA. A novel method relying on liquid solid partitioning of cellular constituents was developed for isolation of total RNA from bacterial cells. This protocol avoids toxic chemicals, and is therefore more suitable for large scale RNA purification than traditional methods. A pair of biotinylated 8-17 DNAzymes was used to bring about the quantitative excision of the 71-nt recombinant RNA from the chimera. The recombinant RNA was isolated by sequence-specific capture on beads with immobilized complementary deoxyoligonucleotide, while DNAzymes were recovered by biotin affinity chromatography for reuse. Conclusions:The feasibility of a fermentation-based approach for manufacturing large quantities of small RNAs in vivo using a "5S rRNA scaffold" strategy is demonstrated. The approach provides a route towards an economical method for the large-scale production of small RNAs including shRNAs, siRNAs and aptamers for use in clinical and biomedical research

DNAzyme-mediated recovery of small recombinant RNAs from a 5S rRNA-derived chimera expressed in Escherichia coli

<p>Abstract</p> <p>Background</p> <p>Manufacturing large quantities of recombinant RNAs by overexpression in a bacterial host is hampered by their instability in intracellular environment. To overcome this problem, an RNA of interest can be fused into a stable bacterial RNA for the resulting chimeric construct to accumulate in the cytoplasm to a sufficiently high level. Being supplemented with cost-effective procedures for isolation of the chimera from cells and recovery of the recombinant RNA from stabilizing scaffold, this strategy might become a viable alternative to the existing methods of chemical or enzymatic RNA synthesis.</p> <p>Results</p> <p>Sequence encoding a 71-nucleotide recombinant RNA was inserted into a plasmid-borne deletion mutant of the <it>Vibrio proteolyticus </it>5S rRNA gene in place of helix III - loop C segment of the original 5S rRNA. After transformation into <it>Escherichia coli</it>, the chimeric RNA (3×<it>pen </it>aRNA) was expressed constitutively from <it>E. coli rrnB </it>P1 and P2 promoters. The RNA chimera accumulated to levels that exceeded those of the host's 5S rRNA. A novel method relying on liquid-solid partitioning of cellular constituents was developed for isolation of total RNA from bacterial cells. This protocol avoids toxic chemicals, and is therefore more suitable for large scale RNA purification than traditional methods. A pair of biotinylated 8-17 DNAzymes was used to bring about the quantitative excision of the 71-nt recombinant RNA from the chimera. The recombinant RNA was isolated by sequence-specific capture on beads with immobilized complementary deoxyoligonucleotide, while DNAzymes were recovered by biotin affinity chromatography for reuse.</p> <p>Conclusions</p> <p>The feasibility of a fermentation-based approach for manufacturing large quantities of small RNAs <it>in vivo </it>using a "5S rRNA scaffold" strategy is demonstrated. The approach provides a route towards an economical method for the large-scale production of small RNAs including shRNAs, siRNAs and aptamers for use in clinical and biomedical research.</p

Thin layer composite unimorph ferroelectric driver and sensor

A method for forming ferroelectric wafers is provided. A prestress layer is placed on the desired mold. A ferroelectric wafer is placed on top of the prestress layer. The layers are heated and then cooled, causing the ferroelectric wafer to become prestressed. The prestress layer may include reinforcing material and the ferroelectric wafer may include electrodes or electrode layers may be placed on either side of the ferroelectric layer. Wafers produced using this method have greatly improved output motion