Dissecting the hydrolytic activities of sarcoplasmic reticulum ATPase in the presence of acetyl phosphate

©2002. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/ This document is the Published version of a Published Work that appeared in final form in The American Society for Biochemistry and Molecular Biolog. To access the final edited and published work see https://doi.org/ 10.1074/jbc.M20396620

L’escola rural. CRA El Trescaire

Dissetenes Jornades de Foment de la Investigació de la FCHS (Any 2012)Ens ha semblat oportú ampliar el concepte d'educació al món rural. En aquest sentit, hem recollit unes pautes que vénen en un llibre editat pel MRP el títol del qual és del tot significatiu: Treballar a l'escola rural ¿Treballar a l'escola rural? ¡Treballar a l'escola rural! Si pensem que possiblement aquest siga el nostre primer destí com a mestres el títol podria ser: Treballar a l'escola rural(m'ha tocat una escola rural) ¿Treballar a l'escola rural?(¡ufh no tinc ni idea que es fa aquí!) ¡Treballar a l'escola rural! (ja estic treballant en una escola rural, ¡és genial!) En aquest llibre figuren algunes de les funcions i objectius fonamentals de l’educació al mon rural, nosaltres mitjançant el nostre treball d’investigació en volgut fer una primera aproximació a aquest mon tan proper i necessari per la futura tasca com docents.We thought it appropriate to extend the concept of education in rural areas. In this regard, we have collected some guidelines that come in a book published by MRP whose title is quite significant: Working at the rural school ¿Work mean? ¡Working in the rural school! If you think this may be our first destination as Masters title could be: Working at the rural school (I have had a rural school) ¿Work mean? (¡Ufh I have no idea what is done here!) ¡Working in the rural school! (I'm working in a rural school, ¡is great!) In this book are some of the fundamental objectives and functions education in rural areas, we work with our research wanted to make a first approach to this world as next and necessary for future work as teachers

Yield and fruit quality response of sweet pepper genotypes grown under soilless cultivation

Total yield, physical, and phytochemical characterization of three yellow and five red-colored sweet pepper genotypes were analyzed under fully controlled environmental and irrigation conditions under soilless culture. Results showed both greater fruit firmness and pericarp thickness in the red-colored genotypes than in the yellow ones. However, no significant differences between these two colors were found for total yield, shape index and dry matter percentage. Additionally, peroxidase activity, total protein and total phenolic compounds were not modified according with the color of the genotype. With respect to the genotypes studied, ‘Cierva’, ‘A67’, ‘Traviatta’, ‘Cabezo’ and ‘Limona’ showed the highest yields in the “extra” fruit category whilst ‘Disco’ and ‘Zar’ showed the lowest. Additionally, ‘Cierva’ and ‘Cabezo’ showed higher protein concentration and peroxidase activity than any other genotype.This research was supported by the Fundación Séneca (Agencia Regional de Ciencia y Tecnología-Región de Murcia) via the project 00554/PI/0

Adsorption of Direct Blue 78 using chitosan and cyclodextrins as adsorbents

The dyeing industry is one of the most polluting in the world. The adsorption of dyes by polymeric matrixes can be used to minimize the discharge of dyes into the environment. In the present study, chitosan-NaOH and β-cyclodextrin-epichlorohydrin polymers were used to remove the dye Direct Blue 78 from a wastewater model. To understand the adsorption behavior of Direct Blue 78 onto the polymers, adsorption rate and maximum adsorption capacity were calculated using kinetic tests and isotherm curves respectively. The kinetic data and mechanism of the adsorption process were analyzed by three models and the equilibrium data by three adsorption isotherms; also the different thermodynamic parameters were calculated. Results showed that the adsorption process follows pseudo-second-order kinetics in both polymers and the Langmuir isotherm best-fitted data for chitosan-NaOH polymer and the Freundlich isotherm for the β-CDs-EPI polymer. The adsorption process is exothermic in both cases and spontaneous for the β-CDs-EPI polymer to a certain temperature and not spontaneous for the chitosan-NaOH polymer and β-CDs-EPI polymer at higher temperatures. The complementary action of an advanced oxidation process eliminated >99% of the dye from water. The coupled process seems to be suitable for reducing the environmental impact of the dyeing industry.Ciencias de la Alimentació

Complexation of resveratrol by native and modified cyclodextrins: Determination of complexation constant by enzymatic, solubility and fluorimetric assays

The complexation of resveratrol with native a-, b- and c-cyclodextrins (CDs) and modified CDs (hydroxypropyl-b-(HP-b-CDs), maltosyl-b-(G2-b-CDs), methyl-b-, carboxymethyl-b- and acetyl-b-cyclodextrins) was studied, and the complexation constants (Kc) were compared. The complexation constant between resveratrol and each type of CD was calculated using three different methods: enzymatic, solubility and fluorimetric. The Kc values obtained showed that HP-b-CDs with their very high Kc of 18,048 ± 625 M 1, were the most effective for complexing resveratrol. Moreover, comparison of the results obtained by the three methods revealed that the fluorimetric method undervalued the Kc between resveratrol and all cyclodextrins, while the enzymatic and solubility methods were more precise for calculating the Kc between resveratrol and CDs, as demonstrated by the cyclodextrin-assay.This work was partially supported by Fundación Séneca (03025/PPC/05) and Ministerio de Educación y Ciencia (AGL2006-08702/ALI). C.L.A. is a holder of a research grant from the Programa Nacional de Formación de Personal Investigador (FPI), Ministerio de Educación y Ciencia (Spain) (BES-2007-16082).Ciencias de la Alimentació

Stability of microencapsulated strawberry flavour by spray drying, freeze drying and fluid bed

The main goal of this paper was to study the stability of a microencapsulated strawberry flavour using different encapsulating agents and drying techniques: spray drying, freeze drying and fluid bed. According to the quantification of volatile compounds, the blend MDs/Hi-Cap (9/1) at a fixed concentration of CDs (1.7%) was the most appropriate for microencapsulating the strawberry flavour. The best drying yield was observed in the case of freeze drying. In the case of the moisture content, spray drying samples presented the lowest values, followed by freeze drying and fluid bed. The study of stability at different temperatures and times revealed that the presence of CDs in the blend enabled a higher presence of volatile compounds in the powder than in its absence. Microphotographs showed smooth spherical particles in the case of spray drying, whereas the structure of the powder was amorphous, like glass, with freeze drying and irregular in the case of fluid bed.Ciencias de la Alimentació

The synergistic action of cyclodextrin-based adsorbent and advanced oxidation processes for sulfamethoxazole removal from water

In this work, the removal of sulfamethoxazole from water was studied by adsorption on a cyclodextrin–epichlorohydrin copolymer and through Advanced Oxidation Processes. The adsorption was efficient and fast, requiring only 10 min to reach the equilibrium; it was also highly favored, with adsorption efficiency higher than 80%, at slightly acidic pH and at room temperature. The desorption of sulfamethoxazole was obtained by raising the temperature to 80 °C. The regenerated polymer has been reused multiple times without any loss in performance, allowing a reduction in costs associated with the process. As an alternative method to regenerate the adsorbent material, the photodegradation of sulfamethoxazole adsorbed on the polymer alone and in the presence of TiO2 as a catalyst through continuous irradiation with UV light and Pulsed Light was attempted and studied. For this purpose, polymeric adsorbents containing different amounts of TiO2 were synthesized and tested. The Pulsed Light showed the highest efficiency since it allowed the complete removal of sulfamethoxazole, regardless of the presence of TiO2 which, however, reduces adsorption efficiency of the material. Furthermore, a qualitative assessment of formed pulsed light by-products was also attempted. The remains of SMX in solution were decomposed by Pulsed Light technology and the effect of the presence of hydrogen peroxide on the photodegradation process was also studied. Despite the presence of by-products after Advanced Oxidation Processes, the procedure proposed in this work is effective and easy to apply to water treatment plants, thanks to the use of environmental-friendly adsorbents and light sources

Afectación hepática y cutánea gomatosas simultánemente en la presentación de sífilis tardía en paciente no coinfectado por el VIH

Far from being eradicated, syphilitic infection is now a growing problem, mainly affecting the young population. Approximately 25-40% of patients with syphilitic infection without treatment, develop late syphilis. Syphilitic involvement of the liver is particularly rare, especially seen in patients with HIV coinfection. We present the case of a 33-year-old patient with tertiary syphilis with skin involvement in the form of gum and liver involvement in the form of microabscesses, which resolved after completing intravenous treatment.Lejos de estar erradicada, la infección sifilítica es hoy en día un problema creciente, que afecta principalmente a la población joven. De los pacientes con infección sifilítica sin tratamiento, aproximadamente entre el 25-40% desarrollan sífilis tardía. Es particularmente extraña la afectación sifilítica del hígado, que se aprecia especialmente en pacientes con coinfección por VIH. Se presenta el caso de un paciente de 33 años con sífilis terciaria con afectación cutánea en forma de goma y hepática en forma de microabscesos, que se resolvió tras completar tratamiento endovenoso

DYRK1A and Activity-Dependent Neuroprotective Protein Comparative Diagnosis Interest in Cerebrospinal Fluid and Plasma in the Context of Alzheimer-Related Cognitive Impairment in Down Syndrome Patients

Down syndrome (DS) is a complex genetic condition due to an additional copy of human chromosome 21, which results in the deregulation of many genes. In addition to the intellectual disability associated with DS, adults with DS also have an ultrahigh risk of developing early onset Alzheimer's disease dementia. DYRK1A, a proline-directed serine/threonine kinase, whose gene is located on chromosome 21, has recently emerged as a promising plasma biomarker in patients with sporadic Alzheimer's disease (AD). The protein DYRK1A is truncated in symptomatic AD, the increased truncated form being associated with a decrease in the level of full-length form. Activity-dependent neuroprotective protein (ADNP), a key protein for the brain development, has been demonstrated to be a useful marker for symptomatic AD and disease progression. In this study, we evaluated DYRK1A and ADNP in CSF and plasma of adults with DS and explored the relationship between these proteins. We used mice models to evaluate the effect of DYRK1A overexpression on ADNP levels and then performed a dual-center cross-sectional human study in adults with DS in Barcelona (Spain) and Paris (France). Both cohorts included adults with DS at different stages of the continuum of AD: asymptomatic AD (aDS), prodromal AD (pDS), and AD dementia (dDS). Non-trisomic controls and patients with sporadic AD dementia were included for comparison. Full-form levels of DYRK1A were decreased in plasma and CSF in adults with DS and symptomatic AD (pDS and dDS) compared to aDS, and in patients with sporadic AD compared to controls. On the contrary, the truncated form of DYRK1A was found to increase both in CSF and plasma in adults with DS and symptomatic AD and in patients with sporadic AD with respect to aDS and controls. ADNP levels showed a more complex structure. ADNP levels increased in aDS groups vs. controls, in agreement with the increase in levels found in the brains of mice overexpressing DYRK1A. However, symptomatic individuals had lower levels than aDS individuals. Our results show that the comparison between full-length and truncated-form levels of DYRK1A coupled with ADNP levels could be used in trials targeting pathophysiological mechanisms of dementia in individuals with DS

Cross-sectional analysis of the medium-term impact of bariatric surgery onpharmacological expenditure

Introduction:Obesity and associated diseases represent an important health and economic problem sincepharmacological treatment for many of these pathologies needs lifelong subsidies. Theoretically, bariatric andmetabolic surgery decreases the medication requirements of patients for these diseases but may result in othertypes of pharmacological needs. This study aims to demonstrate whether there is a real decrease in pharma-cological expenditure after bariatric surgery.Material and methods:Retrospective cross-sectional analysis of patients who were treated in our centre between2012 and 2015, comparing different associated comorbidities and pharmacological expenses one month beforeand 2 years after surgery.Results:A total of 280 patients underwent surgery; 36.8% of patients had diabetes, 50% hypertension, 11.1%cardiovascular disease, 13.9% osteoarticular disease, 13.6% endocrine disorders, 30% non-diabetic metabolicdisorders, and 35.4% psychiatric disease. At 2 years after surgery, 12.1% of patients continued medication fordiabetes, and 28.2% for arterial hypertension. Additionally, 9.3% of patients still had cardiovascular disease,7.1% osteoarticular disease, 10.4% endocrine disorder, 13.9% non-diabetic metabolic disorder, and 29.3%psychiatric disease. Median pharmacological expenditure before surgery was 17 euros per month; 2 years aftersurgery, it was 12 euros a month, resulting in a significant decrease (p < 0.001).Conclusions:In a 2-year follow-up after bariatric surgery, a decreased prevalence of obesity-related diseases andassociated pharmacological expenditure was observed, showing the efficiency of this intervention over themedium term and potentially over the long term