Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species

Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity ranged from 0.376 to 0.844 (mean = 0.698). Two loci exhibited null alleles and all loci were in Hardy-Weinberg equilibrium. No linkage disequilibrium between loci was observed. These loci were used in 4 congeneric species and provide a useful tool for studying population genetics and other aspects of the biology of this and other Anopheles species. ©FUNPEC-RP

Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.

The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium. Eleven loci were cross-amplified successfully in 5 Anopheles species. These microsatellite loci will be useful in studies investigating population structure and evolutionary genetics in A. albitarsis sensu lato and other A. albitarsis complex species

Isolation and characterization of 24 microsatellite loci for the commercial Amazonian freshwater catfish Brachyplatystoma capapretum (Siluriformes: Pimelodidae)

Twenty-four microsatellite loci were characterized for the Pimelodidae Brachyplatystoma capapretum and tested on 26–38 individuals from the Amazon basin. The number of alleles per locus ranged from 1 to 12 with the observed and expected heterozygosity values varying from 0.02 to 0.93 and 0.08 to 0.81, respectively. Twenty-one of the loci were in Hardy–Weinberg equilibrium, and twenty-two were successfully cross-amplified in at least two Brachyplatystoma species. These loci will be usefully to measure the genetic diversity status on B. capapretum specie as in Brachyplatystoma genus. © 2014, Springer Science+Business Media Dordrecht

Polymorphic microsatellite DNA markers for the Amazonian catfish Pseudoplatystoma punctifer (Siluriformes: Pimelodidae)

The large catfish Pseudoplatystoma punctifer has a high commercial value in the Amazon. Fifteen polymorphic microsatellite loci were isolated and characterized in 36-39 individuals of this species sampled at the Purus river (Amazon Basin). The number of alleles per locus ranged from 2 to 18 (average 7.4), the observed heterozygosity (H O) from 0.025 to 0.972 and the expected heterozygosity (H E) from 0.025 to 0.931. Two loci showed a Hardy-Weinberg Equilibrium deviation. No linkage disequilibrium was found among the studied loci. Fourteen microsatellite loci were cross-amplified successfully in six other Pimelodidae species. Therefore, these loci may be valuable for other studies of population genetic structure as well as for catfish conservation in the Amazon basin. © Springer Science+Business Media B.V. 2010

New 24 polymorphic DNA microsatellite loci for the major malaria vector Anopheles darlingi and transpecies amplification with another anophelines

Anopheles darlingi is a major human malaria vector in the Neotropics. Twenty-four polymorphic microsatellite loci were isolated and characterized in 21-32 individuals collected in Coari (Amazonas, Brazil). The number of alleles per locus ranged from 4 to 11 (average of 7.667). The observed heterozygosity (H O) varied between 0.037 and 0.833 (average of 0.500), while the expected heterozygosity (H E) ranged from 0.177 to 0.871 (average of 0.723). Thirteen loci showed a significant deviation from HWE. No linkage disequilibrium was found between the loci. © Springer Science+Business Media B.V. 2010

Genetic diversity and structure of Astrocaryum jauari (Mart.) palm in two Amazon river basins.

Astrocaryum jauari is a non-domesticated palm that is exploited by poachers. Our objective was to investigate the organization of the geneticdiversity and structure of three A. jauari populations. The study was carried out in the state of Amazonas, between the municipalities of Coari and Manaus. Nine microsatellite loci were used for the genetic analyses. High genetic variation was found, with a mean number of alleles per locus varying from 3.9 to 4.4. The average observed heterozygosity, varying from 0.71 to 0.78, was higher than expected. No spatial genetic structure was detected, since only one cluster was observed. Our results indicate a possible dispersion strategy and suggest that conservation measures of this species should focus mainly on the populations found at the end of the main river (Solimões) where most of the plant material originating from the headwaters of the tributaries of this river is concentrated

Implementação da Metodologia 5’S num Setor de Manutenção de uma Indústria Cerâmica

A necessidade de qualquer empresa se adaptar às condições de mercado constitui uma constante e, se adicionarmos o fator globalização, torna-se um imperativo. Face a esta situação, são muitas as empresas que começam a abandonar os métodos de gestão, ditos “tradicionais”, e começam a perseguir novas metodologias, com destaque para o Lean Manufacturing. Neste contexto, assumindo-se como uma das ferramentas elementares da filosofia Lean, a Metodologia 5’S apresenta como primordial objetivo a eliminação do “desperdício”, em paralelo com a criação de um ambiente de trabalho mais organizado, limpo e seguro, contribuindo desta forma, para a otimização dos processos operacionais de uma empresa. A origem do termo “5’S”, fica a dever-se à letra inicial “S” de cinco palavras japonesas que dão o nome a cada uma das etapas da própria metodologia. São elas: Seiri (Classificação e Separação), Seiton (Organização), Seiso (Limpeza), Seiketsu (Estandardização), Shitsuke (Autodisciplina). Neste trabalho, será analisada a capacidade da metodologia 5’S em eliminar o desperdício numa empresa do setor cerâmico tomada como referência, bem como a eficácia da mesma, no estabelecimento de um local de trabalho mais eficiente, confortável, e seguro para os trabalhadores.ABSTRACT: The companies must adapt to business requirements constantly, mainly if the Globalization would be considered as factor. That is why so many companies are starting to follow the Lean Manufacturing philosophy instead of Management traditional methods. Based on this scenario, the main purpose of 5S methodology, classified as one of the most important lean manufacturing tools, is eliminate wasting while provide the work environment clean, organized and safe, in that way, providing operational process improvements. The origin of 5S is regarding to initial letter "s" of five Japanese words which represents every single step of the methodology, which are: Seiri (Classification and Split), Seiton (Organization), Seiso (Cleaning), Seiketsu (Standard) and Shitsuke (Selfdiscipline). During this project, it is going to be analyzed how the 5S methodology can affect in the wasting elimination in a ceramic company taken as a reference, and its efficiency as well to stablish one work environment more efficient, comfortable and safe for employees