Tectonically controlled origin of three unusual rock suites in the Woodlark Basin

We propose alternative mechanisms for the origin of three unusual rock suites, high-Mg andesites, NaTi basalts, and arclike rocks, that have been dredged from the Woodlark basin, southwest Pacific Ocean. We show that the high-Mg andesites and NaTi basalts are associated with an unusually cool ridge environment. The cooling is due to increased hydrothermal circulation, stimulated by an unusually high crustal permeability. The high permeability is mainly due to cracking of the Woodlark basin lithosphere as it passes over the flexural bulge in front of the subduction zone, although local processes, such as faulting in fracture zones, may also make some contribution. This increased convective cooling affects magma dynamics and chemistry at the ridge crest. The high-Mg andesites occur where the hydrothermal circulation lowers the temperatures in the upper oceanic crust, causing the magma chamber to sit in the mantle rather than in the crust and promoting the interaction of basalt magma with harzburgite. The NaTi basalts are also the indirect result of increased crustal cooling, which causes anomalously low degrees of partial melting of their depleted mantle source. The arclike rocks are caused by interaction with volatiles from lithosphere that was emplaced beneath the edge of the basin less than 6 m.y. ago by a now inactive subduction zone to the north of the currently active one. Simple thermal models indicate that this formerly subducting plate is still cold enough to retain volatiles and to remain seismically active. As the old plate loses volatiles, they rise into the mantle convection cell which feeds the Woodlark basin ridge crest. Because ridge subduction increases the likelihood of ophiolite obduction, these observations and explanations of Woodlark basin tectonics are potentially important for ophiolites

Dipolar Evolution in a Coronal Hole Region

Using observations from the SOHO, STEREO and Hinode, we investigate magnetic field evolution in an equatorial coronal hole region. Two dipoles emerge one by one. The negative element of the first dipole disappears due to the interaction with the positive element of the second dipole. During this process, a jet and a plasma eruption are observed. The opposite polarities of the second dipole separate at first, and then cancel with each other, which is first reported in a coronal hole. With the reduction of unsigned magnetic flux of the second dipole from 9.8*10^20 Mx to 3.0*10^20 Mx in two days, 171 A brightness decreases by 75% and coronal loops shrink obviously. At the cancellation sites, the transverse fields are strong and point directly from the positive elements to the negative ones, meanwhile Doppler red-shifts with an average velocity of 0.9 km s-1 are observed, comparable to the horizontal velocity (1.0 km s-1) derived from the cancelling island motion. Several days later, the northeastern part of the coronal hole, where the dipoles are located, appears as a quiet region. These observations support the idea that the interaction between the two dipoles is caused by flux reconnection, while the cancellation between the opposite polarities of the second dipole is due to the submergence of original loops. These results will help us to understand coronal hole evolution.Comment: 23 pages, 9 figures. accepted by Ap

Visuospatial working memory impairment in current and previous ecstasy/polydrug users

Objective: Previous research suggests that ecstasy users are impaired in processing visuospatial information. However, for the most part, the deficits observed appear to involve the recall and recognition of complex visual and geometric patterns. The present research sought to determine whether ecstasy use was associated with deficits in serial spatial recall and visuospatial working memory (VSWM). Methods: Thirty‐eight current ecstasy/polydrug users, 16 previous ecstasy/polydrug users and 52 non ecstasy users completed serial simple spatial recall and VSWM tasks. Results: Both the current and previous users of ecstasy exhibited deficits on the VSWM task. Following controls for group differences in aspects of cannabis and cocaine use, the overall group effect fell to just below statistical significance. However, the difference contrast comparing users with nonusers continued to demonstrate a statistically significant ecstasy‐related VSWM deficit. Conclusions: Ecstasy users were impaired in processing visuospatial information especially under conditions of high processing demand. The results are consistent with ecstasy‐related impairment either in the short‐term posterior parietal and occipital area store or the dorsolateral prefrontal cortex processes, which augment it under conditions of higher processing demands. Further research is needed to pinpoint the actual source of the ecstasy/polydrug‐related VSWM deficits that have been observed here and elsewhere

Application of calibrations to hyperspectral images of food grains: example for wheat falling number

The presence of a few kernels with sprouting problems in a batch of wheat can result in enzymatic activity sufficient to compromise flour functionality and bread quality. This is commonly assessed using the Hagberg Falling Number (HFN) method, which is a batch analysis. Hyperspectral imaging (HSI) can provide analysis at the single grain level with potential for improved performance. The present paper deals with the development and application of calibrations obtained using an HSI system working in the near infrared (NIR) region (~900–2500 nm) and reference measurements of HFN. A partial least squares regression calibration has been built using 425 wheat samples with a HFN range of 62–318 s, including field and laboratory pre-germinated samples placed under wet conditions. Two different approaches were tested to apply calibrations: i) application of the calibration to each pixel, followed by calculation of the average of the resulting values for each object (kernel); ii) calculation of the average spectrum for each object, followed by application of the calibration to the mean spectrum. The calibration performance achieved for HFN (R2 = 0.6; RMSEC ~ 50 s; RMSEP ~ 63 s) compares favourably with other studies using NIR spectroscopy. Linear spectral pre-treatments lead to similar results when applying the two methods, while non-linear treatments such as standard normal variant showed obvious differences between these approaches. A classification model based on linear discriminant analysis (LDA) was also applied to segregate wheat kernels into low (250 s) HFN groups. LDA correctly classified 86.4% of the samples, with a classification accuracy of 97.9% when using HFN threshold of 150 s. These results are promising in terms of wheat quality assessment using a rapid and non-destructive technique which is able to analyse wheat properties on a single-kernel basis, and to classify samples as acceptable or unacceptable for flour production

Ancient metabolisms of a thermophilic subseafloor bacterium

© The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Smith, A. R., Mueller, R., Fisk, M. R., & Colwell, F. S. Ancient metabolisms of a thermophilic subseafloor bacterium. Frontiers in Microbiology, 12, (2021): 764631, https://doi.org/10.3389/fmicb.2021.764631.The ancient origins of metabolism may be rooted deep in oceanic crust, and these early metabolisms may have persisted in the habitable thermal anoxic aquifer where conditions remain similar to those when they first appeared. The Wood–Ljungdahl pathway for acetogenesis is a key early biosynthetic pathway with the potential to influence ocean chemistry and productivity, but its contemporary role in oceanic crust is not well established. Here, we describe the genome of a novel acetogen from a thermal suboceanic aquifer olivine biofilm in the basaltic crust of the Juan de Fuca Ridge (JdFR) whose genome suggests it may utilize an ancient chemosynthetic lifestyle. This organism encodes the genes for the complete canonical Wood–Ljungdahl pathway, but is potentially unable to use sulfate and certain organic carbon sources such as lipids and carbohydrates to supplement its energy requirements, unlike other known acetogens. Instead, this organism may use peptides and amino acids for energy or as organic carbon sources. Additionally, genes involved in surface adhesion, the import of metallic cations found in Fe-bearing minerals, and use of molecular hydrogen, a product of serpentinization reactions between water and olivine, are prevalent within the genome. These adaptations are likely a reflection of local environmental micro-niches, where cells are adapted to life in biofilms using ancient chemosynthetic metabolisms dependent on H2 and iron minerals. Since this organism is phylogenetically distinct from a related acetogenic group of Clostridiales, we propose it as a new species, Candidatus Acetocimmeria pyornia.Metagenome sequencing was made possible by the Deep Carbon Observatory Census of Deep Life supported by the Alfred P. Sloan Foundation and was performed at the Marine Biological Laboratory (Woods Hole, MA, United States). This work was funded by NASA grant NNX08AO22G and a graduate fellowship from the NSF Center for Dark Energy Biosphere Investigations. The flow cells were funded under J0972A from the U.S. Science Support Program of Joint Oceanographic Institutions

Hyperspectral imaging for non-destructive prediction of fermentation index, polyphenol content and antioxidant activity in single cocoa beans

The aim of the current work was to use hyperspectral imaging (HSI) in the spectral range 1000-2500 nm to quantitatively predict fermentation index (FI), total polyphenols (TP) and antioxidant activity (AA) of individual dry fermented cocoa beans scanned on a single seed basis. Seventeen cocoa bean batches were obtained and 10 cocoa beans were used from each batch. PLS regression models were built on 170 samples. The developed HSI predictive models were able to quantify three quality-related parameters with sufficient performance for screening purposes, with external validation R2 of 0.50 (RMSEP=0.27, RPD=1.40), 0.70 (RMSEP=34.1 mg ferulic acid g-1, RPD=1.77) and 0.74 (60.0 mmol Trolog kg-1, RPD=1.91) for FI, TP and AA, respectively. The calibrations were subsequently applied at a single bean and pixel level, so that the distribution was visualised within and between single seeds. HSI is thus suggested as a promising approach to estimate cocoa bean composition rapidly and non-destructively, thus offering a valid tool for food inspection and quality control

Basalt magma interaction with harzburgite and the formation of high-magnesium andesites

The interaction of basalt melt with mantle harzburgite at low pressure might result in silica enrichment of the melt by assimilation of orthopyroxene. Experimental tests of this hypothesis show that silica-rich liquids (56 wt %) are produced by melt-orthopyroxene reaction at 1200° to 1250°C. These silica-rich liquids are enriched in Na₂O and K₂O over that found in the starting basalt melt which indicates that the alkalis diffused against a concentration gradient into the silica-rich liquid. The liquids produced in this way are chemically similar to high-magnesium andesites or boninites

A comprehensive survey of integron-associated genes present in metagenomes

Background: Integrons are genomic elements that mediate horizontal gene transfer by inserting and removing genetic material using site-specific recombination. Integrons are commonly found in bacterial genomes, where they maintain a large and diverse set of genes that plays an important role in adaptation and evolution. Previous studies have started to characterize the wide range of biological functions present in integrons. However, the efforts have so far mainly been limited to genomes from cultivable bacteria and amplicons generated by PCR, thus targeting only a small part of the total integron diversity. Metagenomic data, generated by direct sequencing of environmental and clinical samples, provides a more holistic and unbiased analysis of integron-associated genes. However, the fragmented nature of metagenomic data has previously made such analysis highly challenging. Results: Here, we present a systematic survey of integron-associated genes in metagenomic data. The analysis was based on a newly developed computational method where integron-associated genes were identified by detecting their associated recombination sites. By processing contiguous sequences assembled from more than 10 terabases of metagenomic data, we were able to identify 13,397 unique integron-associated genes. Metagenomes from marine microbial communities had the highest occurrence of integron-associated genes with levels more than 100-fold higher than in the human microbiome. The identified genes had a large functional diversity spanning over several functional classes. Genes associated with defense mechanisms and mobility facilitators were most overrepresented and more than five times as common in integrons compared to other bacterial genes. As many as two thirds of the genes were found to encode proteins of unknown function. Less than 1% of the genes were associated with antibiotic resistance, of which several were novel, previously undescribed, resistance gene variants. Conclusions: Our results highlight the large functional diversity maintained by integrons present in unculturable bacteria and significantly expands the number of described integron-associated genes