A systematic review of team formulations in multidisciplinary teams: staff views and opinions

Background: Formulation and working psychologically with teams is considered a fundamental part of the role of a clinical psychologist. Quantitative studies have found staff find team formulations a positive, useful experience. Aims: This review aims to utilize a Thematic Synthesis to combine qualitative studies on staff experiences of participating in team formulations and explore what benefits staff perceive from the process. The review also aims to provide a thorough quality appraisal of the included research. Method: A Thematic Synthesis was completed on qualitative studies which met the required inclusion criteria. The Critical Appraisal Skills Programme qualitative checklist was used to appraise the included research. Results: The studies included in the review were of a mixed quality, and in some instances there was an insufficient amount of detail to complete an accurate assessment. Overall, seven themes were identified across the studies. Conclusions: The current review highlights the importance of team formulation in general practice for both enhancing staff understanding of service users but also improving professional confidence and validation. The review also highlighted some of the barriers to staff practicing team formulations

Do Anti-Angiogenic VEGF (VEGFxxxb) Isoforms Exist? A Cautionary Tale

Splicing of the human vascular endothelial growth factor-A (VEGF-A) gene has been reported to generate angiogenic (VEGFxxx) and anti-angiogenic (VEGFxxxb) isoforms. Corresponding VEGFxxxb isoforms have also been reported in rat and mouse. We examined VEGFxxxb expression in mouse fibrosarcoma cell lines expressing all or individual VEGF isoforms (VEGF120, 164 or 188), grown in vitro and in vivo, and compared results with those from normal mouse and human tissues. Importantly, genetic construction of VEGF164 and VEGF188 expressing fibrosarcomas, in which exon 7 is fused to the conventional exon 8, precludes VEGFxxxb splicing from occurring. Thus, these two fibrosarcoma cell lines provided endogenous negative controls. Using RT-PCR we show that primers designed to simultaneously amplify VEGFxxx and VEGFxxxb isoforms amplified only VEGFxxx variants in both species. Moreover, only VEGFxxx species were generated when mouse podocytes were treated with TGFβ-1, a reported activator of VEGFxxxb splice selection in human podocytes. A VEGF164/120 heteroduplex species was identified as a PCR artefact, specifically in mouse. VEGFxxxb isoform-specific PCR did amplify putative VEGFxxxb species in mouse and human tissues, but unexpectedly also in VEGF188 and VEGF164 fibrosarcoma cells and tumours, where splicing to produce true VEGFxxxb isoforms cannot occur. Moreover, these products were only consistently generated using reverse primers spanning more than 5 bases across the 8b/7 or 8b/5 splice junctions. Primer annealing to VEGFxxx transcripts and amplification of exon 8b primer ‘tails’ explained the artefactual generation of VEGFxxxb products, since the same products were generated when the PCR reactions were performed with cDNA from VEGF164/VEGF188 ‘knock-in’ vectors used in the generation of single VEGF isoform-expressing transgenic mice from which the fibrosarcoma lines were developed. Collectively, our results highlight important pitfalls in data interpretation associated with detecting VEGFxxxb isoforms using current methods, and demonstrate that anti-angiogenic isoforms are not commonly expressed in mouse or human tissues

Blood salvage device for use during ruptured ectopic pregnancy in low‐resource countries

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135522/1/ijgo74.pd

Beyond Likert ratings: Improving the robustness of developmental research measurement using best-worst scaling

Some of the ‘best practice’ approaches to ensuring reproducibility of research can be difficult to implement in the developmental and clinical domains, where sample sizes and session lengths are constrained by the practicalities of recruitment and testing. For this reason, an important area of improvement to target is the reliability of measurement. Here we demonstrate that best–worst scaling (BWS) provides a superior alternative to Likert ratings for measuring children’s subjective impressions. Seventy-three children aged 5–6 years rated the trustworthiness of faces using either Likert ratings or BWS over two sessions. Individual children’s ratings in the BWS condition were significantly more consistent from session 1 to session 2 than those in the Likert condition, a finding we also replicate with a large adult sample (N = 72). BWS also produced more reliable ratings at the group level than Likert ratings in the child sample. These findings indicate that BWS is a developmentally appropriate response format that can deliver substantial improvements in reliability of measurement, which can increase our confidence in the robustness of findings with children

Neuroinvasion by Mycoplasma pneumoniae in Acute Disseminated Encephalomyelitis

We report the autopsy findings for a 45-year-old man with polyradiculoneuropathy and fatal acute disseminated encephalomyelitis after having Mycoplasma pneumoniae pneumonia. M. pneumoniae antigens were demonstrated by immunohistochemical analysis of brain tissue, indicating neuroinvasion as an additional pathogenetic mechanism in central neurologic complications of M. pneumoniae infection

Monitoring Repair of UV-Induced 6-4-Photoproducts with a Purified DDB2 Protein Complex

Because cells are constantly subjected to DNA damaging insults, DNA repair pathways are critical for genome integrity [1]. DNA damage recognition protein complexes (DRCs) recognize DNA damage and initiate DNA repair. The DNA-Damage Binding protein 2 (DDB2) complex is a DRC that initiates nucleotide excision repair (NER) of DNA damage caused by ultraviolet light (UV) [2]-[4]. Using a purified DDB2 DRC, we created a probe ("DDB2 proteo-probe") that hybridizes to nuclei of cells irradiated with UV and not to cells exposed to other genotoxins. The DDB2 proteo-probe recognized UV-irradiated DNA in classical laboratory assays, including cyto- and histo-chemistry, flow cytometry, and slot-blotting. When immobilized, the proteo-probe also bound soluble UV-irradiated DNA in ELISA-like and DNA pull-down assays. In vitro, the DDB2 proteo-probe preferentially bound 6-4-photoproducts [(6-4)PPs] rather than cyclobutane pyrimidine dimers (CPDs). We followed UV-damage repair by cyto-chemistry in cells fixed at different time after UV irradiation, using either the DDB2 proteo-probe or antibodies against CPDs, or (6-4)PPs. The signals obtained with the DDB2 proteo-probe and with the antibody against (6-4)PPs decreased in a nearly identical manner. Since (6-4)PPs are repaired only by nucleotide excision repair (NER), our results strongly suggest the DDB2 proteo-probe hybridizes to DNA containing (6-4)PPs and allows monitoring of their removal during NER. We discuss the general use of purified DRCs as probes, in lieu of antibodies, to recognize and monitor DNA damage and repair

What makes it so hard to look and to listen? Exploring the use of the Cognitive and Affective Supervisory Approach with children’s social work managers

This paper reports on the findings of an ESRC-funded Knowledge Exchange project designed to explore the contribution of an innovative approach to supervision to social work practitioners’ assessment and decision-making practices. The Cognitive and Affective Supervisory Approach (CASA) is informed by cognitive interviewing techniques originally designed to elicit best evidence from witnesses and victims of crime. Adapted here for use in childcare social work supervision contexts, this model is designed to enhance the quantity and quality of information available for decision-making. Facilitating the reporting of both ‘event information’ and ‘emotion information’, it allows a more detailed picture to emerge of events, as recalled by the individual involved, and the meaning they give to them. Practice supervisors from Children’s Services in two local authorities undertook to introduce the CASA into supervision sessions and were supported in this through the provision of regular reflective group discussions. The project findings highlight the challenges for practitioners of ‘detailed looking’ and for supervisors of ‘active listening’. The paper concludes by acknowledging that the CASA’s successful contribution to decision-making is contingent on both the motivation and confidence of supervisors to develop their skills and an organisational commitment to, and resourcing of, reflective supervisory practices and spaces

Fair winds and following seas remotely: modifying perceptions of fieldwork as a requirement in marine science to aid in diversifying the discipline

Pursuing an academic career in marine science requires a range of skills that can be applied across different contexts, including experimental or computational proficiency, policy engagement, teaching, and seagoing fieldwork. The tendency to advertise careers in marine science with imagery of research expeditions results in the perception that it is a requirement for a career in marine science, an indicator of competitiveness in this discipline. Historically, those participating in remote fieldwork over extended periods of time were perceived as “adventurous explorers, with a strong bias towards western, able-bodied men” (Nash et al., 2019). Use of imagery reinforcing such notions for marine scientists fails to recognize that this perception can be discouraging to individuals from other backgrounds who may be excluded from the discipline by a range of real and perceived participatory barriers. Such exclusionary factors include: caring responsibilities, physical mobility, challenging social environments, isolating and physically uncomfortable working environments, mental health challenges, and access to opportunity (Giles et al., 2020). Such barriers disproportionately affect diverse, underrepresented, and marginalized groups, who may therefore struggle to identify with marine science as a potential discipline in which to pursue a successful career. Current work toward achieving net zero targets within ocean research emphasizes the use of autonomous vehicles as alternatives to ocean-going ships (Storey, 2023), and the proposed concept of digital twinning would incorporate similar remote technology coupled with simulations and shore-based decision-making. The concept of digital twinning refers to the use of responsive autonomous platforms that can both collect data and be operated in response to that data, which could provide a non-field-based approach to delivering marine science while also potentially expanding the opportunities available for individuals not able or interested in working in the field. In distinguishing digital twinning from current approaches such as data assimilating models, Kritzinger et al. (2018) note the importance of a two-way data flow between the physical environment and its virtual representation, called a “digital twin,” which, for example, may lead to changes in deployment strategy or data collection by researchers. Because these twins can be controlled and simulated anywhere with access to sufficient computing power, shore-based individuals can interact with a virtual version of the physical environment without being physically present at sea. The technology to support a fully realized digital twin of the ocean is still under development, but its use would require a broader range of skills and roles in the discipline, many of which are not accurately conveyed by the prevailing marketing of field-based disciplines (see Mol and Atchinson, 2019, regarding geosciences). In order to fully integrate this new approach into marine science, employment of individuals with experience and training across a wide range of disciplines from software engineering to traditional field sampling is essential while also presenting the potential for making marine science more inclusive. Individuals for whom working at sea is not possible and/or desirable would be able to make equally valid contributions to such research projects via digital routes, without facing the many barriers fieldwork may present. This study explores the expectations of marine scientists, from both early and more established career stages, around the importance of field experience as a precursor or requirement for a successful marine science career, and also examines the advantages and disadvantages of using digital twinning as a complement to traditional field-based marine science