Does the heat generated by fluorescence-aided caries excavation system effect the pulp temperature of primary teeth irreversibly? An in-vitro evaluation of the temperature changes in the pulp chamber

This study aimed to analyze the effect of the Fluorescence Aided Caries Excavation (FACE) and the remaining dentin thickness on the temperature changes of the pulp chamber. Freshly extracted deciduous molars and a pulpal microcirculation model were use

The Effect of Restoration Polymerization and Residual Dentine Thickness on Thermal Changes of Pulp Chamber of Immature Permanent Teeth

Objective: To evaluate the pulpal temperature changes due to the polymerisation of resin and glass ionomer-based materials in dentine thicknesses in immature permanent teeth with open apices. Material and Methods: Forty extracted sound human third molar teeth with open apices were included. The width of the cavities prepared on the occlusal surface was 4×5 mm. The depth was 2 mm in the resin groups. 4 mm in the groups in which glass ionomer liner was applied before composite restoration. The coronal parts of the samples were then placed on an acrylic plate with three gaps for feeding-extraction needles and the thermocouple. The temperature changes were recorded. The data was analyzed by SPSS. Statistical significance was accepted as p<0.05. Results: The temperature increase in the group of 1 mm remaining dentin thickness revealed higher results than the values detected from the 2 mm group (1.01 °C) (p=0.00). The mean values (1.49 °C, 1mm) of temperature changes in only glass ionomer applied group  were lower than the avarage values (2.210°C, 1mm) determined in the polymerization process of resin composites with light-emitting diode devices. Conclusion: In a remaining dentin thickness of 1 mm in teeth with open apices, using a glass ionomer liner might be a useful effort for protecting the pulp from the heat generated by polymerisation devices

BETTER WITH OZONE, OR NOT? AN IN-VIVO STUDY OF OZONE THERAPY AS A PRE-TREATMENT BEFORE FISSURE SEALANT APPLICATION.

Objectives: The aim of the study was to evaluate clinical achievements of ClinproTMSealant and Teeth mate F-1 fissure sealants with or without ozone treatment as well as retention, marginal discoloration, marginal adaptation and caries formation under in-vivo conditions. Materials and Methods: The study was carried out on 166 patients whose ages differs between 9-12 and 3 different groups were formed above them according to split mouth technique, in order to evaluate clinical achievements of fissure sealants with or without ozone treatment. Group 1: ClinproTMSealant and Teethmate F-1 were applied on a total of 104 first lower permanent molar teeth which were randomly selected from 52 patients. Group 2: ClinproTM Sealant and ClinproTM Sealant following ozone treatment were applied on 112 randomly selected teeth of 56 patients. Group 3: Teeth mate F-1 and Teeth mate F-1 following the ozone treatment were applied on 116 first lower permanent molar teeth which were randomly selected from 58 patients. Based on the modified USPHS criteria, the impacts on general achievement, retention, marginal fit, marginal discoloration and caries formation on occlusal surfaces were evaluated in 3rd,6th,12th,18th months. Chi-Square Test and Fisher’s Exact Test were used for analyses of the data. Results: In Group 1; Clinpro™Sealant showed higher clinical success rates in all observation periods. Considering marginal adaptation and retention, Clinpro™Sealant group was statistically significantly different in 18th month observations. In group 2 and group 3, there was not statistically significantly difference (p>0.05) between control and experimental groups but clinical success rates of Clinpro ™ Sealant and Teetmate F-1 with Ozone Treatment were higher than the same groups without ozone pretreatment during all observation periods. Conclusion: It was determined that, ClinproTMSealant yielded more successful results than the Teethmate F-1 under in-vivo conditions. Although there wasn’t any statistically significantly difference between Group 2 and Group 3 in which Ozone as a pretreatment method before performing fissure sealant was considered, the Ozone pretreatment method’s success rates were higher clinically according to Bravo, Alpha and Charlie Scores. It seems that performing Ozone Therapy before applying fissure sealant is a successful method for preventing pit and fissure sealants, clinically

Changing indications and techniques for corneal transplantations at a tertiary referral center in Turkey, from 1995 to 2014

BACKGROUND: Indications for corneal transplantation in developed and developing nations differ according to the different spectrum of corneal disease in each country. OBJECTIVE: The purpose of this study is to analyze the changing indications and surgical techniques for corneal transplantation over the past 20 years at a tertiary referral center in Turkey. METHODS: We retrospectively reviewed the records of patients who underwent keratoplasty from January 1995 to December 2014 (between 1995 and 2004, period 1, and between 2005 and 2014, period 2). Patients’ demographic data, indications for corneal transplantation, and the type of surgery were recorded. RESULTS: The number of keratoplasties performed ranged from 548 in period 1 to 782 in period 2. Between 1995 and 2004, the leading indications were keratoconus (34.1%), bullous keratopathy (17%), and non-herpetic corneal scar (13.3%), and between 2005 and 2014, they were keratoconus (33.8%), corneal stromal dystrophy (14.2%), and bullous keratopathy (12.7%). All the keratoplasties performed in the 1995–2004 period were penetrating keratoplasty (PKP). During the period 2005–2014, PKP accounted for 93%, automated lamellar keratoplasty 5.8%, and deep anterior lamellar keratoplasty 1.2% of all corneal transplantations. CONCLUSION: Keratoconus was the leading indication for keratoplasty in both periods. In the 2005–2014 period, corneal stromal dystrophy increased significantly. All the keratoplasties performed in period 1 and 93% of all keratoplasties performed in period 2 were PKP

COMPARISON OF CLINICAL, LABORATORY FINDINGS AND COMPLICATIONS IN BACTERIEMIC AND NONBACTERIEMIC BRUCELLOSIS: A SINGLE CENTER EXPERIENCE

Objective: Brucellosis is a systemic zoonosis that affects various organs or body systems and can mimic many diseases. Symptoms can range from acute febrile illness to chronic clinical manifestations with severe complications. In our study, we investigated the relationship between bacteremia and clinical and laboratory findings, and complications in patients with brucellosis. Material and Methods: Two hundred and twenty-three patients diagnosed with brucellosis between 2005 and 2020 in the department of infectious diseases and clinical microbiology of a tertiary care university hospital were included in the study. Rose Bengal test, Brucella standard tube agglutination test and culture (blood and/or bone marrow culture) were used for the diagnosis of brucellosis. The patients were divided into two groups according to their bacteremia status. Patients with positive culture were identified as bacteremia, and patients with negative culture were identified as non-bacteriemic. Results: A total of 223 patients were included in the study, of which 142 (63.7%) were male. While weight loss, fever and hematological complications were higher in bacteremia cases (p=0.001,

Diagnostic and Prognostic Value of SCUBE-1 in COVID-19 Patients

Introduction: The workload of physicians increased due to the number of patients presenting with suspicion of coronavirus 2019 (COVID-19) and the prolonged wait times in the emergency department during the COVID-19 pandemic. Signal peptide-CUB-EGF domain-containing protein 1 (SCUBE-1) is a protein present in platelets and endothelial cells; it is activated by inflammation from COVID-19 and may be associated with COVID-19’s known thrombotic risk. We aimed to determine whether SCUBE-1 levels are diagnostically correlated in suspected COVID-19 patients, and whether SCUBE-1 correlated with severity of disease and, therefore, might be useful to guide hospitalization/discharge decisions. Methods: The suspected COVID-19 patients cared for at tertiary healthcare institutions for one year between May 2021–May 2022 were examined in this study. The subjects were both suspected COVID-19 patients not ultimately found to have COVID-19 and those who were diagnosed with COVID-19. By modifying the disease severity scoring systems present in COVID-19 guidelines in 2021, the COVID-19-positive patient group was classified as mild, moderate, severe, and critical, and compared using the SCUBE-1 levels. Moreover, SCUBE-1 levels were compared between the COVID-19 positive group and the COVID-19 negative group. Results: A total of 507 patients were considered for the present study. After excluding 175 patients for incomplete data and alternate comorbid organ failure. we report on 332 patients (65.5%). Of these 332 patients, 80 (24.0%) were COVID-19 negative, and 252 (76.0%) were COVID-19 positive. Of 252 (100%) patients diagnosed with COVID-19, 74 (29.4%) were classified as mild, 95 (37.7%) moderate, 45 (17.8%) severe, and 38 (15.1%) critical. The SCUBE-1 levels were statistically different between COVID-19 positive (8.48 ± 7.42 nanograms per milliliter [ng/mL]) and COVID-19 negative (1.86 ± 0.92 ng/mL) patients (P < 0.001). In the COVID-19 positive group, SCUBE-1 levels increased with disease severity (mild = 3.20 ± 1.65 ng/mL, moderate = 4.78 ± 2.26 ng/mL, severe = 13.68 ± 3.95 ng/mL, and critical = 21.87 ± 5.39 ng/mL) (P < 0.001). The initial SCUBE-1 levels of discharged patients were significantly lower than those requiring hospitalization (discharged = 2.89 ng/mL [0.55–8.60 ng/mL]; ward admitted = 7.13 ng/mL [1.38–21.29 ng/mL], and ICU admitted = 21.19 ng/mL [10.58–37.86 ng/mL]) (P < 0.001). Conclusion: The SCUBE-1 levels were found to be differentiated between patients with and without COVID-19 and to be correlated with the severity of illness

Different epidemiology of bloodstream infections in COVID-19 compared to non-COVID-19 critically ill patients: A descriptive analysis of the Eurobact II study

Background: The study aimed to describe the epidemiology and outcomes of hospital-acquired bloodstream infections (HABSIs) between COVID-19 and non-COVID-19 critically ill patients. Methods: We used data from the Eurobact II study, a prospective observational multicontinental cohort study on HABSI treated in ICU. For the current analysis, we selected centers that included both COVID-19 and non-COVID-19 critically ill patients. We performed descriptive statistics between COVID-19 and non-COVID-19 in terms of patients’ characteristics, source of infection and microorganism distribution. We studied the association between COVID-19 status and mortality using multivariable fragility Cox models. Results: A total of 53 centers from 19 countries over the 5 continents were eligible. Overall, 829 patients (median age 65 years [IQR 55; 74]; male, n = 538 [64.9%]) were treated for a HABSI. Included patients comprised 252 (30.4%) COVID-19 and 577 (69.6%) non-COVID-19 patients. The time interval between hospital admission and HABSI was similar between both groups. Respiratory sources (40.1 vs. 26.0%, p < 0.0001) and primary HABSI (25.4% vs. 17.2%, p = 0.006) were more frequent in COVID-19 patients. COVID-19 patients had more often enterococcal (20.5% vs. 9%) and Acinetobacter spp. (18.8% vs. 13.6%) HABSIs. Bacteremic COVID-19 patients had an increased mortality hazard ratio (HR) versus non-COVID-19 patients (HR 1.91, 95% CI 1.49–2.45). Conclusions: We showed that the epidemiology of HABSI differed between COVID-19 and non-COVID-19 patients. Enterococcal HABSI predominated in COVID-19 patients. COVID-19 patients with HABSI had elevated risk of mortality. Trial registration ClinicalTrials.org number NCT03937245. Registered 3 May 2019

Uluabat Gölü ötrifikasyon kontrolü için maksimum alıcı ortam fosfor yüklerinin belirlenmesi

TÜBİTAK ÇAYDAG15.03.2006Bu çalışma, Uluabat Gölü'ne giden fosfor yüklerinin belirlenmesini amaçlamaktadır. Kalibrasyonu takiben, SWAT ile sediman ve fosfor taşımını modellenmiştir. Modelde kulandan arazi kullanımı ve diğer koşullar için göle yıllık 4 grP/m /yıl'lık bir fosfor yükü ulaştığını sonucuna varılmıştır. Uygulanan gübre miktarı, Emet ve Orhaneli havzalarından gelen yükler ve Uluabat Gölü etrafındaki tarımsal alanların varlığı, göle giden fosfor yüklerini etkilediği sonucuna varılmıştır. Bu faktörlerde yapılan iyileştirmeler sonucunda göle giden fosfor yükünün yarı yarıya azaltılabileceği hesaplanmıştır. Ancak, bu değer hedeflenen 1.0 gP/m2/yıl'dan fazladır Havza için oluşturulan fosfor indeksi, çalışılan havzanın güney kısımları hariç, diğer tüm tarım yapılan alanlarda yüksek fosfor taşınım riski olduğunu göstermiştir. Yağış miktarı ve gübreleme metodu, bu riski arttıran en önemli faktörler olarak ortaya çıkmıştır. Gölde yapılan analizler, yaz aylarında alg, ÇO ve besin maddesi konsantrasyonlarının arttığını göstermiştir. Sınırlı sayıdaki ölçümler gölün fosfor kısıtlı olduğunu göstermektedir ancak toplam azotun toplam fosfora oranı nispeten sınırda olduğu için bu kuvvetli bir kısıtı göstermemektedir. WASP ile elde edilen sonuçlar, fosfor yükündeki %50'lik bir azalmanın gölde gözlenen P konsantrasyonlarını önemli ölçüde azaltabileceğini göstermiştir. Ancak sonuçların anlamlı olabilmesi için sediman aktivitesinin de modellenmesi gerekmektedir