Mechanistic behaviour and molecular interactions of heat shock protein 47 (HSP47)

This project involves the study of heat shock protein 47 (HSP47), which is a molecular chaperone crucial for collagen biosynthesis. It exhibits a high degree of sequence homology with members of the serine protease inhibitor (serpin) superfamily, though HSP47 does not possess the inhibitory activity. It is a single-substrate chaperone, and binds only to collagen. ‘Knock-out’ of the hsp47 gene impairs the secretion of correctly folded collagen triple helix molecules leading to embryonic lethality in mice. Thus the aim of this project was to elucidate the specific mechanism that governs the binding to and release from collagen at the molecular level, known as the ‘pH-switch mechanism’. Emphasis is given on histidine (His) residues as the HSP47-collagen dissociation pH is similar to the pKa of the imidazole side chain of His residues. Site directed mutagenesis was used to mutate surface His residues, based on a mouse HSP47 homology model. The effects of the mutations on the behaviour of HSP47 were then assessed by collagen binding assays and structural analyses with circular dichroism (CD). All mutants were found to have good solubility and retain their binding ability to collagen like wild-type HSP47 in batch assay, but perturbed behaviour was seen in column experiment. Mutation of His residue at position 191 (H191) causes the shift in the collagen dissociation pH, while mutation of H197 and/or 198 disrupt the specific HSP47-collagen interaction. H191, 197 and 198 are predicted to be located in the region near the C-terminus of strand 3 of β-sheet A (s3A) in the homology model, a region specifically known as the ‘breach cluster’ in serpin nomenclature. The extent of conformational rearrangement of this region was further investigated by means of intrinsic tryptophan fluorescence spectroscopy using a series of single tryptophan (Trp) mutants. Results from analyses performed on the mutants did not contradict the observation seen in His mutational work, as Trp residues in the ‘breach’ cluster are likely to be located in the dynamic region of HSP47 pH-triggered conformational change. In conclusion, this study establishes the importance of His residues in the ‘breach cluster’ to HSP47 pH-switch behaviour. Finally, a model for HSP47 pH-switch mechanism was proposed from data obtained via mutagenesis experiments. The model is hoped to assist future research into HSP47 cellular behaviour and will also be of great use in therapeutic applications involving the molecular chaperone

PENGARUH PERBEDAAN SUHU INLET FLUIDA TERHADAP KALOR YANG DILEPAS DAN EFISIENSI HEAT EXCHANGER (The Effect of Fluid Inlet Temperature Difference to The Released Heat and The Efficiency of Heat Exchanger)

Heat exchanger is an equipment to exchange heat between two kinds of fluid or more. As a device to exchange heat from hot fluid to colder fluid, with a variation of the inlet temperature of the hot fluid and cold can affect the amount of heat that is released and of course the efficiency of the heat exchanger can also be seen

PERSONAL BRANDING INFLUENCER PADA MEDIA SOSIAL TIKTOK (Analisis isi Pesan Personal Branding pada Akun @shaturday)

Shadira Firdausi is one of the influencers on TikTok social media who uses TikTok social media as a place to build personal branding. Shadira's TikTok account presents DIY and a day in my life content that is uniquely packaged in her style. The @shaturday account has been followed by 4.1 million followers. This research aims to describe the personal branding carried out by Shadira Firdausi through her TikTok social media account. Researchers use the theory of The Eight Laws of Personal Branding by Peter Montoya, where there are 8 concepts in building personal branding. Researchers also use the basic characteristics that must be built when forming personal branding according to Montoya & Vandehey, namely you, promise, and relationship as well as video elements in describing Shadira's personal branding on TikTok social media. Peter Montoya defines personal branding as a process that requires unique skills, personality and characteristics packaged in a strong identity, so that it can be remembered by the public. This research uses a qualitative approach with a descriptive research type and a qualitative content analysis research basis. This research uses documentation techniques in the form of screenshots from the @shaturday account with DIY themed videos. This data was then analyzed using interactive data analysis developed by Miles and Huberman. The research results show that the personal branding built by Shadira Firdausi through her TikTok social media account @shaturday prioritizes Shadira, who is seen as an aesthetic and candid influencer. In the content presented, Shadira uses 3 video elements, namely text, images and sound. Shadira has also met the basic criteria for building personal branding, namely you, promise, and relation

ANALISIS STRATEGI MARKETING COMMUNICATION DALAM MENINGKATKAN OKUPANSI DI GRAND MERCURE MALANG MIRAMA

In the hotel industry, marketing communications is an important component in the marketing strategy of a good or service. The type of research used by researchers is descriptive research with a qualitative approach. The data collection techniques used were observation, interviews and documentation. The informants taken for data analysis were assistant marketing communications manager, guest experience manager, revenue executive at Grand Mercure Malang Mirama. Grand Mercure Malang Mirama's marketing strategy is running well because of a plan and marketing support activities which are driven by integrated marketing communication. The results of this research are about the marketing communication strategy carried out by Grand Mercure Malang Mirama which is quite effective but needs ongoing evaluation. The marketing communication activities carried out by Grand Mercure Malang Mirama are media advertising, public relations, relationship management. customers (Customer Relationship Management), sales promotions (sales promotions), direct sales (personal selling), direct marketing (direct marketing), direct experiences to customers (event & experience). customer relationship management (customer relationship management). Keywords: marketing strategy, integrated marketing, hote

Optimalisasi pengembangan kurikulum fasilitasi berbasis kompetensi pada fasilitas peningkatan kompetensi guru dalam melaksanakan penelitian tindakan kelas

Tujuan penulisan artikel ini adalah mendeskripsikan proposal perkonsultasian fasilitasi sebagai rancangan bahan konsultasi berkaitan dengan pengembangan kurikulum fasilitasi berbasis kompetensi. Fasilitasi yang dimaksud merupakan fasilitasi yang diselenggarakan atau dilaksanakan oleh Lembaga Penjaminan Mutu Pendidikan (LPMP) Provinsi Sumatera Selatan, baik secara mandiri maupun bekerja sama dengan pihak lain. Secara khusus, proposal perkonsultasian fasilitasi ini membahas penerapan model ADDIE untuk mengoptimalkan pengembangan kurikulum fasilitasi berbasis kompetensi pada fasilitasi peningkatan kompetensi guru dalam melaksanakan penelitian tindakan kelas. Kat

Kepemimpinan Kepala Sekolah Dalam Meningkatkan Kualitas Sumber Daya Manusia (Studi Kasus Di Smp Muhammadiyah 8 Surakarta Tahun Pelajaran 2011/2012)

Kepala sekolah merupakan salah satu komponen pendidikan yang paling berperan dalam meningkatkan mutu pendidikan di sekolahnya. Keberhasilan suatu sekolah terletak pada kemampuan pimpinan sekolah dalam mengelola semua sumber daya yang tersedia. Oleh karena itu seorang pimpinan sekolah yang profesional sebagai usaha meningkatkan mutu SDM dalam pendidikan adalah sebuah keniscayaan yang harus terpenuhi. SDM yang harus ditingkatkan adalah seperti sikap profesional guru dan karyawan, baik secara kognitif, afektif, dan psikomotorik agar dapat menjalankan tugas-tugasnya secara efektif dan efisien. SMP Muhammadiyah 8 Surakarta termasuk salah satu sekolah swasta dengan terakreditasi A. Sekolah ini memiliki kelebihan dalam pembelajaran yang menerapkan sistem fullday school, serta kurikulum sekolah yang memadukan antara kurikulum Depdiknas dan kurikulum Dikdasmen Muhammadiyah. Sekolah yang menerapkan sistem pembelajaran fullday school diharapkan memiliki tenaga pengajar yang lebih profesional dan berkualitas. Kepemimpinan kepala sekolah diharapkan dapat mengedepankan visi dan misi, mengutamakan musyawarah, serta menghargai pendapat dari anggotanya. Maka dari itu, kepala sekolah perlu mendayagunakan SDM secara optimal, sehingga mampu mewujudkan tujuan dan sasaran sekolah. Permasalahan dalam penelitian ini adalah bagaimana kepemimpinan kepala sekolah di SMP Muhammadiyah 8 Surakarta, usaha dalam meningkatkan SDM, serta apa saja problem yang muncul dalam meningkatkan kualitas SDM dan solusi mengatasinya. Tujuan dari penelitian ini adalah untuk mendeskripsikan kepemimpinan kepala sekolah, usaha-usaha dalam meningkatkan SDM, serta problem yang muncul dalam peningkatan SDM dan solusi mengatasinya. Jenis penelitian ini adalah penelitian kualitatif dengan pendekatan deskriptif. Subjek penelitian ini adalah kepala sekolah, guru dan karyawan. Sedangkan teknik pengumpulan data dengan menggunakan metode wawancara, observasi, dan dokumentasi, yang kemudian dianalisis dengan menggunakan metode deskriptif kualitatif. Hasil dari penelitian ini adalah kepemimpinan kepala sekolah terbukti efektif dengan adanya tiga corak gaya kepemimpinan. Usaha-usaha dalam peningkatan SDM melalui MGMP, pengajian, diskusi, pelatihan-pelatihan serta pemberian gaji dan reward. Adapun problem yang muncul adalah 1) belum menyatunya visi dan misi antar guru, solusinya pihak sekolah mengadakan rapat dan pendekatan individual secara khusus; 2) minimnya arahan dan pengawasan, solusinya adalah dengan melakukan pendekatan personal dan memberikan pengawasan secara rutin melalui kunjungan kelas; 3) masalah finansial, solusinya adalah komite sekolah mencarikan dana shadaqah, serta menaikkan SPP bagi yang mampu

LEARNING DESCRIPTIVE WRITING WITH LOCAL TOURISM DESTINATION PICTURES

English is internasional language that used by people in around the world in any levels. So it is studied start form elementary school until college, but they found difficult to study English. Its become a problem for the students or learners  because English is foreign language. Moreover, in our country English is not first or second languge but it is as a foreign language. Teacher should find the batter ways to improve students' English mastery or skill. Making a teaching material, teacher could be do anythings  to improve students' English skill include using a Local Tourism Destination Pictures to improve students English skills especialy in descriptive writing. By using a tool like a picture is make easy for the students to make a hand writing and improve it become a good English writing skill, so using a teaching media like a picture is very effective to improve students’ writing English mastery

EDUCATION, INTENSITY OF MOTHER’S PERCEPTION SENSORY STIMULATION WITH LANGUAGE DEVELOPMENT OF CHILDREN

Introduction: Stimulation is an important factor in the children’s growth and development. According to NHCS-National Center for Health Statistics (2000) in United States, 3.29% of children which is less than 5 years old still have the language development delay. Therefore, the purpose of this study is to analyze the corcorrelation between the education and the intensity of perception sensory stimulation given by the carers and the language development speed of children whose age is 9 – 12 months in integrated health post of Durung Bedug Baru, Sidoarjo (Integrated Health Post). Method: The design of the study used was a correlative analytic with cross sectional. The population involved 40 mothers and their children’s age was 9 – 12 months. 36 respondents were chosen as the samples by using simple random sampling technique. Questionnaires and DDST (Denver Development Screening Test) were used to collect the data which were then analyzed by using Spearman’s Rank statistic test. Moreover, based on the statistic test, the result showed that ρ = 0.00 < α = 0.05 so that H0 was rejected, it described that there was a corcorrelation between the mother’s education and the language development, and ρ = 0.011 meant that p < α = 0.05. Result: H0 was rejected, it described that there was a corcorrelation between the intensity of carer’s perception sensory stimulation and the language development speed of children whose aged were 9 – 12 months. Discussion: Low education and lack of giving intensity of perception sensory stimulation causes the children have an abnormal language development. Keywords: Education, Perception Sensory, Language Development

Effect of Total Members and Current Ratio on the Rest of Cooperative Business Result

This study aims to analyze the effect of variable number of members and current ratio on the remaining business results (SHU). The sample used is all data for the time series period 2012-2018. The data used are secondary data and the method used is multiple linear regression analysis with the help of SPSS Version 23 program to obtain the influence of the independent variables with the dependent variable. The results showed that the number of members had a positive and significant effect on the residual results of operations, the current ratio had a negative and significant effect on the residual results of operations. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp