661 research outputs found
Assessment of Identity Disturbance: Factor Structure and Validation of the Personality Structure Questionnaire in an Italian Sample
here are few brief measures of identity disturbance for use in clinical
practice
that have
been subject to any cross
-
culturally validation
. This study investigated the construct
validity of the Personality Structure Questionnaire (PSQ)
in Italian
clinical (N=237) and
community (
N=296) samples
. Confirmatory factor analysis was conducted to
investigate the internal structure of the PSQ. A three
–
factor structure (i.e., differing self
-
states, mood variability and behavioral loss of control) including
a second
-
order factor
provided the best fit to the data
. This structure was
demonstrated to be invariant across
sex
and
clinical
diagnosis
, with clinical diagnosis significantly predicting increased
PSQ
scores
.
A g
lobal
PSQ score
of
between 26
-
28 was found to be an appropriate cut-off for
assisting in diagnostic processes. Implications for the assessment and treatment of
psychological disorders with a marked identity disturbance component are discussed
Ambulatory surgery for perianal Crohn’s disease. Study of feasibility
Background. One-third of Crohn’s disease (CD) patients present perianal fistula. The gold standard in the diagnosis and treatment of symptomatic perianal disease (PAD) in CD is the exploration of the anal canal and distal rectum under anesthesia (EUA). This procedure is mainly conducted as a day case surgery. Unfortunately, it is not always possible to proceed within the ideal timing and any delay may well represent a relevant clinical issue. The aim of this study was to evaluate the feasibility of outpatient treatment of symptomatic perianal fistulas in CD patients. Methods. All CD patients under regular follow-up at our inflammatory bowel disease referral center, presenting with symptomatic perianal fistulas, were offered surgical consultation. The data of patients were prospectively collected for three years (February 2014 to February 2017) for the purpose of the study. All clinical information, including previous EUA and/or records from MRI and endoscopic ultrasound, was included. Outpatient anal canal and distal rectum exploration and treatment (OE) were undertaken during the specialist surgical consultation. Fistulas were classified according to Parks’s classification; the type of outpatient treatment and compliance of patients were recorded. Pain was assessed by VAS at the time of the procedure and during the first control. Patients were followed up in the surgical clinic in relation to the study. Results. Ninety-two CD patients with symptomatic perianal fistulas had surgical consultation during the study period. OE was offered to all but 18 patients who fulfilled the exclusion criteria or had an extremely severe disease; six patients refused the OE (8.11%). Of the 68 patients undergoing OE, eleven (16.18%) had previous surgery for perianal disease. The OE was accomplished in sixty-one patients (89.71%), while in 7 patients, it was abandoned for scarce compliance. Nine patients (14.75%) underwent drainage of perianal abscess; in 3 of them, it was possible to probe the fistula tract, find the internal orifice, and pass a loose seton. Overall, setonage was performed in 50 patients (81.97%). Rectovaginal setons were placed in 3 patients and more than one seton (up to 3) in 6 cases. Fistulotomy was performed in 4 simple subcutaneous fistulous tracts. Concordance with the preoperative findings was found in 54 out of 61 patients. EUA was scheduled at the time of OE for the 7 patients who did not complete the procedure. All sixty-one patients who had the OE were followed up for a minimum of 12 months. Conclusions. This preliminary study indicates that OE in CD patients with symptomatic perianal fistulas is safe and feasible in a high-volume referral center. It might provide several benefits, including patients’ logistics, reduce or remove patients’ symptoms and discomfort, allow for a timely start of medical therapy, and avoid further complications
Topoisomerase IB: a relaxing enzyme for stressed DNA
DNA topoisomerase I enzymes relieve the torsional strain in DNA; they are essential for fundamental molecular processes such as DNA replication, transcription, recombination, and chromosome condensation; and act by cleaving and then religating DNA strands. Over the past few decades, scientists have focused on the DNA topoisomerases biological functions and established a unique role of Type I DNA topoisomerases in regulating gene expression and DNA chromosome condensation. Moreover, the human enzyme is being investigated as a target for cancer chemotherapy. The active site tyrosine is responsible for initiating two transesterification reactions to cleave and then religate the DNA backbone, allowing the release of superhelical tension. The different steps of the catalytic mechanism are affected by various inhibitors; some of them prevent the interaction between the enzyme and the DNA while others act as poisons, leading to TopI-D NA lesions, breakage of DNA, and eventually cellular death. In this review, our goal is to provide an overview of mechanism of human topoisomerase IB action together with the different types of inhibitors and their effect on the enzyme functionality
Magnetic properties of cobalt ferrite-silica nanocomposites prepared by a sol-gel autocombustion technique
The magnetic properties of cobalt ferrite-silica nanocomposites with different concentrations (15, 30, and 50 wt %) and sizes (7, 16, and 28 nm) of ferrite particles have been studied by static magnetization measurements and Mossbauer spectroscopy. The results indicate a superparamagnetic behavior of the nanoparticles, with weak interactions slightly increasing with the cobalt ferrite content and with the particle size. From high-field Mossbauer spectra at low temperatures, the cationic distribution and the degree of spin canting have been estimated and both parameters are only slightly dependent on the particle size. The magnetic anisotropy constant increases with decreasing particle size, but in contrast to many other systems, the cobalt ferrite nanoparticles are found to have an anisotropy constant that is smaller than the bulk value. This can be explained by the distribution of the cations. The weak dependence of spin canting degree on particle size indicates that the spin canting is not simply a surface phenomenon but also occurs in the interiors of the particles. (c) 2006 American Institute of Physics
Magnetic properties of the frustrated AFM spinel ZnCr_2O_4 and the spin-glass Zn_{1-x}Cd_xCr_2O_4 (x=0.05,0.10)
The -dependence (2- 400 K) of the electron paramagnetic resonance (EPR),
magnetic susceptibility, , and specific heat, , of the
antiferromagnetic (AFM) spinel ZnCrO and the spin-glass
(SG) ZnCdCrO () is reported. These
systems behave as a strongly frustrated AFM and SG with K and -400 K K. At high-
the EPR intensity follows the and the -value is -independent.
The linewidth broadens as the temperature is lowered, suggesting the existence
of short range AFM correlations in the paramagnetic phase. For
ZnCrO the EPR intensity and decreases below 90 K and 50
K, respectively. These results are discussed in terms of nearest-neighbor
Cr (S %) spin-coupled pairs with an exchange coupling of 50 K. The appearance of small resonance modes for K,
the observation of a sharp drop in and a strong peak in
at K confirms, as previously reported, the existence of long range
AFM correlations in the low- phase. A comparison with recent neutron
diffraction experiments that found a near dispersionless excitation at 4.5 meV
for and a continuous gapless spectrum for ,
is also given.Comment: 17 pages, 8 figures, 1 Table. Submitted to Physical Review
. New ground-based lidar enables volcanic CO2 flux measurements
There have been substantial advances in the ability to monitor the activity of hazardous volcanoes
in recent decades. However, obtaining early warning of eruptions remains challenging, because the
patterns and consequences of volcanic unrests are both complex and nonlinear. Measuring volcanic
gases has long been a key aspect of volcano monitoring since these mobile fluids should reach the
surface long before the magma. There has been considerable progress in methods for remote and
in-situ gas sensing, but measuring the flux of volcanic CO2—the most reliable gas precursor to an
eruption—has remained a challenge. Here we report on the first direct quantitative measurements
of the volcanic CO2 flux using a newly designed differential absorption lidar (DIAL), which were
performed at the restless Campi Flegrei volcano. We show that DIAL makes it possible to remotely
obtain volcanic CO2 flux time series with a high temporal resolution (tens of minutes) and accuracy
(<30%). The ability of this lidar to remotely sense volcanic CO2 represents a major step forward
in volcano monitoring, and will contribute improved volcanic CO2 flux inventories. Our results also
demonstrate the unusually strong degassing behavior of Campi Flegrei fumaroles in the current
ongoing state of unrest
Particle size dependence of the magnetic properties of ultrafine granular films
Magnetic granular solids consist of ultrafine metal granules of nanometer sizes embedded in an insulating medium. The magnetic properties are dictated by the microstructure such as particle size, metal volume fraction, which are process controlled. We report the results of a series of granular Fe60(SiO2)40 films in which the particle size has been systematically varied. The magnetic coercivity, ranging from 500 to 2200 Oe, increases with particle size. The ferromagnetic-superparamagnetic transition had been studied by SQUID magnetometry and Mössbauer spectroscopy. The value of the magnetic anisotropy energy is found to be much larger than that due to magnetocrystalline anisotropy
A small organic compound enhances the religation reaction of human topoisomerase I and identifies crucial elements for the religation mechanism
The different steps of the human Top1 (topoisomerase I) catalytic cycle have been analysed in the presence of a
pentacyclic-diquinoid synthetic compound. The experiments indicate that it efficiently inhibits the cleavage step of
the enzyme reaction, fitting well into the catalytic site. Surprisingly the compound, when incubated with the binary
topoisomerase–DNA cleaved complex, helps the enzyme to remove itself from the cleaved DNA and close the DNA gap,
increasing the religation rate. The compound also induces the religation of the stalled enzyme–CPT (camptothecin)–
DNA ternary complex. Analysis of the molecule docked over the binary complex, together with its chemical properties,
suggests that the religation enhancement is due to the presence on the compound of two oxygen atoms that act as
hydrogen acceptors. This property facilitates the deprotonation of the 5 DNA end, suggesting that this is the limiting
step in the topoisomerase religation mechanism
Survey on the effectiveness of telephone-based communication with relatives of hospitalized cancer patients in COVID-19 era in Italy
Objective: No-visitor policies adopted to prevent coronavirus disease-19 (COVID-19) spread in hospital wards have deeply impacted communication with patients and their relatives. Whereas in pre-COVID-19 era family-clinician meetings were held in person, during the pandemic interactions often took place over the phone, frequently causing feelings of uncertainty and distress to the close ones at home. The goal of this study was to assess and improve the effectiveness of structured telephone-based communication with hospitalized onco-hematological patients’ relatives in COVID-19 era. Methods: After no-visitor policy was adopted in the Onco-Hematological Unit of Modena, inpatients’ relatives were contacted daily for clinical updates. After discharge, a telephone satisfaction survey was administered to all contact people of patients consecutive admitted between December 2020 and January 2021 (n = 97). Mean score of response and potential statistically significative differences depending on respondents’ characteristics were assessed. Results: Most relatives were satisfied with the communication received with a mean total score of 4.69 on a 5-point Likert scale (standard deviation: 0.60). Results showed high satisfaction rate with both the informative (mean ± SD: 4.66 ± 0.64) and emotional (mean ± SD: 4.66 ± 0.58) content, with no significant difference depending on respondents’ demographic characteristics (p > 0.05). Conclusion: A structured telephone-based communication may be a reasonable substitute for face-to-face meetings; especially if regular in time, conducted by the same doctor and integrated with video calls. Our findings might assist health workers in implementing measures to minimize the psychological effects of no-visitor policies during hospitalization. Clinical updates delivery through structured phone calls and video calls could become an opportunity also in post-COVID era
In vitro and in silico characterization of an antimalarial compound with antitumor activity targeting human DNA topoisomerase IB
Human DNA topoisomerase IB controls the topological state of supercoiled DNA through a complex catalytic cycle that consists of cleavage and religation reactions, allowing the progression of fundamental DNA metabolism. The catalytic steps of human DNA topoisomerase IB were analyzed in the presence of a drug, obtained by the open-access drug bank Medicines for Malaria Venture. The experiments indicate that the compound strongly and irreversibly inhibits the cleavage step of the enzyme reaction and reduces the cell viability of three different cancer cell lines. Molecular docking and molecular dynamics simulations suggest that the drug binds to the human DNA topoisomerase IB-DNA complex sitting inside the catalytic site of the enzyme, providing a molecular explanation for the cleavage-inhibition effect. For all these reasons, the aforementioned drug could be a possible lead compound for the development of an efficient anti-tumor molecule targeting human DNA topoisomerase IB
- …