Virtual slit scanning microscopy

We present a novel slit scanning confocal microscope with a CCD camera image sensor and a virtual slit aperture for descanning that can be adjusted during post-processing. A very efficient data structure and mathematical criteria for aligning the virtual aperture guarantee the ease of use. We further introduce a method to reduce the anisotropic lateral resolution of slit scanning microscopes. System performance is evaluated against a spinning disk confocal microscope on identical specimens. The virtual slit scanning microscope works as the spinning disk type and outperforms on thick specimen

Widefield fluorescence microscopy with extended resolution

Widefield fluorescence microscopy is seeing dramatic improvements in resolution, reaching today 100nm in all three dimensions. This gain in resolution is achieved by dispensing with uniform Köhler illumination. Instead, non-uniform excitation light patterns with sinusoidal intensity variations in one, two, or three dimensions are applied combined with powerful image reconstruction techniques. Taking advantage of non-linear fluorophore response to the excitation field, the resolution can be further improved down to several 10nm. In this review article, we describe the image formation in the microscope and computational reconstruction of the high-resolution dataset when exciting the specimen with a harmonic light pattern conveniently generated by interfering laser beams forming standing waves. We will also discuss extensions to total internal reflection microscopy, non-linear microscopy, and three-dimensional imagin

Perancangan Sistem Dan Denah Bazaar Dengan Memanfaatkan Canvas HTML5 Berbasis Website Dan Android

At present, almost every organizers holds an attractive bazaar due to the growing number of enthusiasts who want to join bazaar. Bazaar can be an alternative place to sell new product and introduce the local product to the general society. Booking booth process in bazaar still using manual system, which tenant fills the order form and then the form is scanned to be sent to the organizer of the event via e-email. Once the booking is received, event organizer has to check and make a registration list of tenants whom will join the bazaar. Therefore there is a need to have an information system to help both eventorganizer and tenant to make a better booking process. Aplication Bazaar based on Android and website intends to help user do all booking process efficiently.Application based on mobile assist tenant in making reservation from fill form until payment order. It helps tenant to search for information about the bazaar which will be held in accordance with bazaar categories of interest and can follow event, so tenant will be easier to get bazaar information. In addition, application based on website helps event organizer to manage the whole process from booking to the payment which received from tenant order, then event organizer can edit event map in order to facilitate tenant when choose a booth at the bazaar.The test results which obatained have two modules, Mobile Application based on Android for tenant and application website for event organizer. Mobile Application can make a booking in bazaar, can get a notification, follow the event, displaying order data. Application website for the event organizer can manage the whole order and payment from tenant, and can edit map event to help tenant make an order. From the test of mobile application that have been done, 50% of respondents rate the app is overall good, and 42% of respondent rate the app overall very good. For Website Application, event organizer has satified with the existing features on the website

Converting lateral scanning into axial focusing to speed up three-dimensional microscopy.

Funder: MedImmune, and Infinitus (China) Ltd.In optical microscopy, the slow axial scanning rate of the objective or the sample has traditionally limited the speed of volumetric imaging. Recently, by conjugating either a movable mirror to the image plane in a remote-focusing geometry or an electrically tuneable lens (ETL) to the back focal plane, rapid axial scanning has been achieved. However, mechanical actuation of a mirror limits the axial scanning rate (usually only 10-100 Hz for piezoelectric or voice coil-based actuators), while ETLs introduce spherical and higher-order aberrations that prevent high-resolution imaging. In an effort to overcome these limitations, we introduce a novel optical design that transforms a lateral-scan motion into a spherical aberration-free axial scan that can be used for high-resolution imaging. Using a galvanometric mirror, we scan a laser beam laterally in a remote-focusing arm, which is then back-reflected from different heights of a mirror in the image space. We characterize the optical performance of this remote-focusing technique and use it to accelerate axially swept light-sheet microscopy by an order of magnitude, allowing the quantification of rapid vesicular dynamics in three dimensions. We also demonstrate resonant remote focusing at 12 kHz with a two-photon raster-scanning microscope, which allows rapid imaging of brain tissues and zebrafish cardiac dynamics with diffraction-limited resolution

Evaluation of flight efficiency for Stockholm Arlanda Airport arrivals

Analysis of punctuality of airport arrivals, as well as identification of causes of the delays within transition airspace, is an important step in evaluating performance of the Terminal Maneuvering Area (TMA) Air Navigation Services: without knowing the current performance levels, it is difficult to identify which areas could be improved. Deviations from the flight plans is one of the major reasons for arrival delays. In this work, we quantified the impact of the deviations from the flight plans on the fuel burn. One of the main reasons of fuel waste is non- optimal vertical profiles during the descent phase. We calculated how much extra fuel is wasted due to vertical flight inefficiency within Stockholm TMA.Peer ReviewedPostprint (published version