25 research outputs found
Aerosol Mixing State: Measurements, Modeling, and Impacts
Atmospheric aerosols are complex mixtures of different chemical species, and individual particles exist in many different shapes and morphologies. Together, these characteristics contribute to the aerosol mixing state. This review provides an overview of measurement techniques to probe aerosol mixing state, discusses how aerosol mixing state is represented in atmospheric models at different scales, and synthesizes our knowledge of aerosol mixing stateâs impact on climateârelevant properties, such as cloud condensation and ice nucleating particle concentrations, and aerosol optical properties. We present these findings within a framework that defines aerosol mixing state along with appropriate mixing state metrics to quantify it. Future research directions are identified, with a focus on the need for integrating mixing state measurements and modeling.Key PointsWe define aerosol mixing state and connect it to the physicochemical properties of aerosol particlesWe discuss existing measurements and models to understand chemical and physicochemical mixing stateWe explain the connection between aerosol mixing state and climateârelevant aerosol propertiesPeer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/150540/1/rog20184_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/150540/2/rog20184.pd
A nonsense mutation in the beta-carotene oxygenase 2 (BCO2) gene is tightly associated with accumulation of carotenoids in adipose tissue in sheep (Ovis aries)
<p>Abstract</p> <p>Background</p> <p>Sheep carcasses with yellow fat are sporadically observed at Norwegian slaughter houses. This phenomenon is known to be inherited as a recessive trait, and is caused by accumulation of carotenoids in adipose tissue. Two enzymes are known to be important in carotenoid degradation in mammals, and are therefore potential candidate genes for this trait. These are <it>beta-carotene 15,15'-monooxygenase 1 (BCMO1) </it>and the <it>beta-carotene oxygenase 2 (BCO2)</it>.</p> <p>Results</p> <p>In the present study the coding region of the <it>BCMO1 </it>and the <it>BCO2 </it>gene were sequenced in yellow fat individuals and compared to the corresponding sequences from control animals with white fat. In the yellow fat individuals a nonsense mutation was found in <it>BCO2 </it>nucleotide position 196 (<it>c.196C>T</it>), introducing a stop codon in amino acid position 66. The full length protein consists of 575 amino acids. In spite of a very low frequency of this mutation in the Norwegian AI-ram population, 16 out of 18 yellow fat lambs were found to be homozygous for this mutation.</p> <p>Conclusion</p> <p>In the present study a nonsense mutation (<it>c.196C>T</it>) in the <it>beta-carotene oxygenase 2 (BCO2) </it>gene is found to strongly associate with the yellow fat phenotype in sheep. The existence of individuals lacking this mutation, but still demonstrating yellow fat, suggests that additional mutations may cause a similar phenotype in this population. The results demonstrate a quantitatively important role for BCO2 in carotenoid degradation, which might indicate a broad enzyme specificity for carotenoids. Animals homozygous for the mutation are not reported to suffer from any negative health or development traits, pointing towards a minor role of BCO2 in vitamin A formation. Genotyping AI rams for <it>c.196C>T </it>can now be actively used in selection against the yellow fat trait.</p
Beta-Carotene Reduces Body Adiposity of Mice via BCMO1
Evidence from cell culture studies indicates that β-carotene-(BC)-derived apocarotenoid signaling molecules can modulate the activities of nuclear receptors that regulate many aspects of adipocyte physiology. Two BC metabolizing enzymes, the BC-15,15â˛-oxygenase (Bcmo1) and the BC-9â˛,10â˛-oxygenase (Bcdo2) are expressed in adipocytes. Bcmo1 catalyzes the conversion of BC into retinaldehyde and Bcdo2 into β-10â˛-apocarotenal and β-ionone. Here we analyzed the impact of BC on body adiposity of mice. To genetically dissect the roles of Bcmo1 and Bcdo2 in this process, we used wild-type and Bcmo1-/- mice for this study. In wild-type mice, BC was converted into retinoids. In contrast, Bcmo1-/- mice showed increased expression of Bcdo2 in adipocytes and β-10â˛-apocarotenol accumulated as the major BC derivative. In wild-type mice, BC significantly reduced body adiposity (by 28%), leptinemia and adipocyte size. Genome wide microarray analysis of inguinal white adipose tissue revealed a generalized decrease of mRNA expression of peroxisome proliferator-activated receptor Îł (PPARÎł) target genes. Consistently, the expression of this key transcription factor for lipogenesis was significantly reduced both on the mRNA and protein levels. Despite β-10â˛-apocarotenoid production, this effect of BC was absent in Bcmo1-/- mice, demonstrating that it was dependent on the Bcmo1-mediated production of retinoids. Our study evidences an important role of BC for the control of body adiposity in mice and identifies Bcmo1 as critical molecular player for the regulation of PPARÎł activity in adipocyte
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Biomass burning aerosols in most climate models are too absorbing
Uncertainty in the representation of biomass burning (BB) aerosol composition and optical properties in climate models contributes to a range in modeled aerosol effects on incoming solar radiation. Depending on the model, the top-of-the-atmosphere BB aerosol effect can range from cooling to warming. By relating aerosol absorption relative to extinction and carbonaceous aerosol composition from observational datasets to nine state-of-the-art Earth System Models/Chemical Transport Models,we identify varying degrees of overestimation in BB aerosol absorptivity by these models. Modifications to BB aerosol refractive index, size, and mixing state improve the Community Atmosphere Model version 5 (CAM5) agreement with observations, leading to a global change in BB direct radiative effect of -0.07 W m-2,and regional changes of -2 W m-2(Africa) and -0.5 W m-2(South America/Temperate). Our findings suggest that current modeled BB contributes less to warming than previously thought, largely due to treatments of aerosol mixing state
Explaining variance in black carbon's aging timescale
The size and composition of particles containing black carbon (BC) are
modified soon after emission by condensation of semivolatile substances and
coagulation with other particles, known collectively as "aging" processes.
Although this change in particle properties is widely recognized, the
timescale for transformation is not well constrained. In this work, we
simulated aerosol aging with the particle-resolved model PartMC-MOSAIC (Particle Monte Carlo â Model for Simulating Aerosol Interactions and Chemistry) and
extracted aging timescales based on changes in particle cloud condensation
nuclei (CCN). We simulated nearly 300 scenarios and, through a regression
analysis, identified the key parameters driving the value of the aging
timescale. We show that BC's aging timescale spans from hours to weeks,
depending on the local environmental conditions and the characteristics of
the fresh BC-containing particles. Although the simulations presented in this
study included many processes and particle interactions, we show that 80% of
the variance in the aging timescale is explained by only a few key
parameters. The condensation aging timescale decreased with the flux of
condensing aerosol and was shortest for the largest fresh particles, while
the coagulation aging timescale decreased with the total number concentration
of large (<i>D</i> >100 nm), CCN-active particles and was shortest for the smallest
fresh particles. Therefore, both condensation and coagulation play important
roles in aging, and their relative impact depends on the particle size range
Shaping the online fat acceptance movement: talking about body image and beauty standards
Mobile TV
This article explores how mobile television is being constructed and understood, focusing on four concepts used in contemporary public debate to discuss the technology, namely 'TV in your pocket', 'TV anytime, anywhere', 'TV on the go', and 'Enhanced TV'. Drawing on an analysis of industry reports, conference proceedings, websites, academic studies, press coverage, results of trials, advertisements and expert interviews, we examine the ways in which experts involved in the production, marketing, delivery and analysis of mobile TV regard this emergent technology. It is argued that mobile TV is constructed by these experts as a novel technological and cultural experience and form, while at the same time the rhetoric of novelty is paralleled with a continuous emphasis on the new medium's relation to familiar technological worlds. The article concludes by offering an explanation for this new/old articulation of mobile TV