Control of Phomopsis Blight of Eastern Redcedar with Benomyl

Control of Phomopsis blight (P. juniperovora) of eastern redcedar (Juniperus virginiana) with benomyl was investigated using potted seedlings in a greenhouse. Benomyl sprays and drenches did not prevent infection. However, three pre-inoculation foliar sprays (600 ppm a.i.) applied at weekly intervals followed by three post-inoculation sprays gave significant control of disease progression. Benomyl drenches of 0, 6.5, 13, 40, 80, 160, 320 and 640 mg/liter pot resulted in significant blight control with the 40 to 640 mg treatments. Thin layer chromatography coupled with Penicillium bioassay determined that 3 μg of benomyl (MBC)/g plant tissue (fresh weight) was the minimal concentration necessary to limit disease progression. Fungitoxic activity was also determined biweekly in nursery grown 2-0 eastern redcedar receiving benomyl applications of 0.6 kg/ha biweekly, 1.4 kg/ha monthly, or 2.8 kg/ha every 6 weeks. Analysis for systemically transported benomyl (MBC) revealed concentrations to be less than the minimal amount necessary for disease control as indicated by the greenhouse experiments. However, the percentage of trees with pycnidia and the percentage of pycnidia with spores were both significantly lower in the benomyl treatments

Phytoremediation of Saline-Sodic Soils in East Central South Dakota Utilizing Perennial Grass Mixtures

Several decades of above average precipitation in South Dakota has increased the area of saline and sodic soils, which reduce crop yields and inhibit sensitive plant growth. Saline and sodic soils are difficult to remediate using traditional agricultural crops. Establishing salt tolerant perennial species may restore productivity to salt affected areas. Two perennial grass mixtures (mix 1: slender wheatgrass, beardless wildrye; mix 2: slender wheatgrass, western wheatgrass, green wheatgrass, creeping meadow foxtail) were dormant frost seeded along a topographic gradient in Clark Co., SD. Soils were Forman-Cresbard loam and a Cresbard-Cavour loam with surface electrical conductivity (EC1:1) that ranged from \u3c 0.5 to \u3e15 dS m-1 and sodium \u3c 400 to \u3e2500 ug g-1. Perennial grass and corn biomass, soil chemical properties, weed cover, and greenhouse gas emissions were quantified in two growing seasons (2018 and 2019). By 2019 perennial grass mixtures and corn reduced soil EC1:1 and sodium similarly. Slender wheatgrass was the dominant species in mix 1, comprising up to 65% of September 2018 total biomass (246-1705 kg ha-1) and 83% in 2019 (6400-9700 kg ha-1). AC Saltlander was the dominant species in mix 2 comprising up to 61% of 2018 total September biomass (604- 2646 kg ha-1) and 81% in July, 2019 (5853-10663 kg ha-1). In July, 2019 mix 1 and mix 2 saline plots had 16% and 3% weed cover (kochia/foxtail barley), respectively, compared to 75% in corn. Over a 7 d period in July, 2018 and 2019 non-fertilized barren saline soils emitted 2.09 and 4.89 g N2O-N ha-1 hr-1, respectively; and 611 and 324 g CO2-C ha-1 hr-1, respectively. During the same time period, grass vegetated non-saline soil emitted 0.38 and 0.46 g N2O-N ha-1 hr-1 in 2018 and 2019, respectively; and 1589 and 2538 g CO2-C ha-1 hr-1, respectively. Corn vegetated non-saline soil emitted 0.01 and 0.62 g N2O-N ha-1 hr-1 in 2018 and 2019, respectively; and 1821 and 1812 g CO2-C ha-1 hr-1, respectively. Urea application (224 kg-1 ha-1) increased CO2 emissions in all treatments both years from 19-155%, but increased N2O emissions by 102-704% in 2019 only. Simulated root exudates, plant residue decomposition, simulated root respiration, and barley growth increased greenhouse gas emissions compared with nontreated controls in laboratory studies on a saline Cresbard-Cavour loam. Growing barley plants reduced soil EC1:1 from 6.3 dS m-1 to 5.9 dS m-1 and reduced soil NO3- from 509 ug g-1 to 428 ug g-1 after 7 weeks and increased N2O-N and CO2-C flux by 224% and 244%, respectively, from baselines of 0.359 ug N2O-N kg-1 hr-1 and 206 ug CO2-C kg-1 hr-1. Revegetating salt affected soils with perennial grasses may reduce soil EC1:1, NO3-, Na, and weed cover and also may improve soil microbial activity and nutrient cycling

By their words ye shall know them: Language abstraction and the likeability of describers

According to the linguistic category model (LCM), behaviour can be described at concrete (e.g. ‘Kath hit Kim’) and abstract (e.g. ‘Kath is aggressive’) levels. Variations in these levels convey information about the person being described and the relationship between that person and the describer. In the current research, we examined the power of language abstraction to create impressions of describers themselves. Results show that describers are seen as less likeable when they use abstract (vs. concrete) language to describe the negative actions of others. Conversely, impressions of describers are more favourable when they opt for abstract descriptions of others' positive behaviours. This effect is partially mediated by the attribution of a communicative agenda to describers. By virtue of these attributional implications, language abstraction is an impression formation device that can impact on the reputation of describers

Methodological strategies in using home sleep apnea testing in research and practice

Purpose Home sleep apnea testing (HSAT) has increased due to improvements in technology, accessibility, and changes in third party reimbursement requirements. Research studies using HSAT have not consistently reported procedures and methodological challenges. This paper had two objectives: (1) summarize the literature on use of HSAT in research of adults and (2) identify methodological strategies to use in research and practice to standardize HSAT procedures and information. Methods Search strategy included studies of participants undergoing sleep testing for OSA using HSAT. MEDLINE via PubMed, CINAHL, and Embase with the following search terms: “polysomnography,” “home,” “level III,” “obstructive sleep apnea,” and “out of center testing.” Results Research articles that met inclusion criteria (n = 34) inconsistently reported methods and methodological challenges in terms of: (a) participant sampling; (b) instrumentation issues; (c) clinical variables; (d) data processing; and (e) patient acceptability. Ten methodological strategies were identified for adoption when using HSAT in research and practice. Conclusions Future studies need to address the methodological challenges summarized in this paper as well as identify and report consistent HSAT procedures and information

Can Phytoremediation-Induced Changes in the Microbiome Improve Saline/Sodic Soil and Plant Health?

Increasing soil salinity and/or sodicity is an expanding problem in the Northern Great Plains (NGP) of North America. This study investigated the impact of phytoremediation on the soil microbiome and if changes, in turn, had positive or negative effects on plant establishment. Amplicon sequencing and gas chromatograph/mass spectrometer analysis compared root metabolites and microbial composition of bulk vs. rhizosphere soils between two soil types (productive and saline/sodic). Beta-diversity analysis indicated that bacterial and fungal communities from both the bulk and rhizosphere soils from each soil type clustered separately, indicating dissimilar microbial composition. Plant species also influenced both root-associated bacterial and fungal communities with separate clustering of operational taxonomic units (OTUs) identified. Canonical correlation analysis (CCA) found a clear association between specific soil characteristics and soil types. Bacterial and fungal OTUs from productive soil were correlated with greater %Ca Sat, %H Sat, and potassium (ppm), especially for OTUs differentially enriched in productive soil. Both bacterial and fungal OTUs from saline/sodic soil are associated with increased Ca (ppm), soil pH, %Na Sat and CEC. Metabolite analysis showed that kochia (Bassia scoparia) roots from the saline/sodic soil had a 4.4-fold decrease in pantothenate accumulation (p = 0.004). Moreover, two endophytic bacterial isolates, a Bacillus spp. and a previously uncultured halophile, isolated from creeping foxtail (Alopecurus arundinaceus) grown in saline/sodic soil and used as buckwheat (Fagopyrum esculentum) seed inoculants, significantly increased seed germination by \u3e30% and vigor index by 0.2 under osmotic stress (0.2 M NaCl) (p \u3c 0.05). This study revealed the importance of soil, root-associated, and endophytic microbiomes. Using native microbes as seed inoculants may help in establishment and growth of species used for phytoremediation of saline/sodic soil

Open ocean dead zones in the tropical North Atlantic Ocean

Here we present first observations, from instrumentation installed on moorings and a float, of unexpectedly low (<2 μmol kg−1) oxygen environments in the open waters of the tropical North Atlantic, a region where oxygen concentration does normally not fall much below 40 μmol kg−1. The low-oxygen zones are created at shallow depth, just below the mixed layer, in the euphotic zone of cyclonic eddies and anticyclonic-modewater eddies. Both types of eddies are prone to high surface productivity. Net respiration rates for the eddies are found to be 3 to 5 times higher when compared with surrounding waters. Oxygen is lowest in the centre of the eddies, in a depth range where the swirl velocity, defining the transition between eddy and surroundings, has its maximum. It is assumed that the strong velocity at the outer rim of the eddies hampers the transport of properties across the eddies boundary and as such isolates their cores. This is supported by a remarkably stable hydrographic structure of the eddies core over periods of several months. The eddies propagate westward, at about 4 to 5 km day−1, from their generation region off the West African coast into the open ocean. High productivity and accompanying respiration, paired with sluggish exchange across the eddy boundary, create the "dead zone" inside the eddies, so far only reported for coastal areas or lakes. We observe a direct impact of the open ocean dead zones on the marine ecosystem as such that the diurnal vertical migration of zooplankton is suppressed inside the eddies

Clinical benefit of glasdegib plus low-dose cytarabine in patients with de novo and secondary acute myeloid leukemia: long-term analysis of a phase II randomized trial

This analysis from the phase II BRIGHT AML 1003 trial reports the long-term efficacy and safety of glasdegib + low-dose cytarabine (LDAC) in patients with acute myeloid leukemia ineligible for intensive chemotherapy. The multicenter, open-label study randomized (2:1) patients to receive glasdegib + LDAC (de novo, n = 38; secondary acute myeloid leukemia, n = 40) or LDAC alone (de novo, n = 18; secondary acute myeloid leukemia, n = 20). At the time of analysis, 90% of patients had died, with the longest follow-up since randomization 36 months. The combination of glasdegib and LDAC conferred superior overall survival (OS) versus LDAC alone; hazard ratio (HR) 0.495; (95% confidence interval [CI] 0.325–0.752); p = 0.0004; median OS was 8.3 versus 4.3 months. Improvement in OS was consistent across cytogenetic risk groups. In a post-hoc subgroup analysis, a survival trend with glasdegib + LDAC was observed in patients with de novo acute myeloid leukemia (HR 0.720; 95% CI 0.395– 1.312; p = 0.14; median OS 6.6 vs 4.3 months) and secondary acute myeloid leukemia (HR 0.287; 95% CI 0.151–0.548; p < 0.0001; median OS 9.1 vs 4.1 months). The incidence of adverse events in the glasdegib + LDAC arm decreased after 90 days’ therapy: 83.7% versus 98.7% during the first 90 days. Glasdegib + LDAC versus LDAC alone continued to demonstrate superior OS in patients with acute myeloid leukemia; the clinical benefit with glasdegib + LDAC was particularly prominent in patients with secondary acute myeloid leukemia. ClinicalTrials.gov identifier: NCT01546038

100% RAG: Architectural Education | THE SCHOOLS, Volume 2, Number 5

100% RAG: Architectural Education | THE SCHOOLS, Syracuse School of Architecture, Student Newspaper, Volume 2, Number 5. Student newsletter from student contributors of Syracuse School of Architecture in 1977

The T-type calcium channel isoform Ca v 3.1 is a target for the hypnotic effect of the anaesthetic neurosteroid (3β,5β,17β)-3-hydroxyandrostane-17-carbonitrile

BACKGROUND: The mechanisms underlying the role of T-type calcium channels (T-channels) in thalamocortical excitability and oscillations in vivo during neurosteroid-induced hypnosis are largely unknown. METHODS: We used patch-clamp electrophysiological recordings from acute brain slices ex vivo, recordings of local field potentials (LFPs) from the central medial thalamic nucleus in vivo, and wild-type (WT) and Ca RESULTS: Patch-clamp recordings showed that 3β-OH inhibited isolated T-currents but had no effect on phasic or tonic γ-aminobutyric acid A currents. Also in acute brain slices, 3β-OH inhibited the spike firing mode more profoundly in WT than in Ca CONCLUSIONS: The C

Impact of Venetoclax and Azacitidine in Treatment-Naïve Patients with Acute Myeloid Leukemia and IDH1/2 Mutations

partially_open16Purpose: To evaluate efficacy and safety of venetoclax + azacitidine among treatment-naïve patients with IDH1/2-mutant (mut) acute myeloid leukemia (AML). Patients and methods: Data were pooled from patients enrolled in a phase III study (NCT02993523) that compared patients treated with venetoclax + azacitidine or placebo + azacitidine and a prior phase Ib study (NCT02203773) where patients were treated with venetoclax + azacitidine. Enrolled patients were ineligible for intensive therapy due to age ≥75 years and/or comorbidities. Patients on venetoclax + azacitidine received venetoclax 400 mg orally (days 1-28) and azacitidine (75 mg/m2; days 1-7/28-day cycle). Results: In the biomarker-evaluable population, IDH1/2mut was detected in 81 (26%) and 28 (22%) patients in the venetoclax + azacitidine and azacitidine groups. Composite complete remission [CRc, complete remission (CR)+CR with incomplete hematologic recovery (CRi)] rates (venetoclax + azacitidine/azacitidine) among patients with IDH1/2mut were 79%/11%, median duration of remission (mDoR) was 29.5/9.5 months, and median overall survival (mOS) was 24.5/6.2 months. CRc rates among patients with IDH1/2 wild-type (WT) were 63%/31%, mDoR 17.5/10.3 months, and mOS 12.3/10.1 months. In patients with IDH1mut, CRc rates (venetoclax + azacitidine/azacitidine) were 66.7%/9.1% and mOS 15.2/2.2 months. In patients with IDH2mut, CRc rates were 86.0%/11.1% and mOS not reached (NR)/13.0 months. Patients with IDH1/2 WT AML treated with venetoclax + azacitidine with poor-risk cytogenetics had inferior outcomes compared with patients with IDH1/2mut, who had superior outcomes regardless of cytogenetic risk (mOS, IDH1/2mut: intermediate-risk, 24.5 months; poor-risk, NR; IDH1/2 WT: intermediate, 19.2 and poor, 7.4 months). There were no unexpected toxicities in the venetoclax + azacitidine group. Conclusions: Patients with IDH1/2mut who received venetoclax + azacitidine had high response rates, durable remissions, and significant OS; cytogenetic risk did not mitigate the favorable outcomes seen from this regimen for IDH1/2mut.partially_openembargoed_20230131Pollyea, Daniel A; DiNardo, Courtney D; Arellano, Martha L; Pigneux, Arnaud; Fiedler, Walter; Konopleva, Marina; Rizzieri, David A; Smith, B Douglas; Shinagawa, Atsushi; Lemoli, Roberto M; Dail, Monique; Duan, Yinghui; Chyla, Brenda; Potluri, Jalaja; Miller, Catherine L; Kantarjian, Hagop MPollyea, Daniel A; Dinardo, Courtney D; Arellano, Martha L; Pigneux, Arnaud; Fiedler, Walter; Konopleva, Marina; Rizzieri, David A; Smith, B Douglas; Shinagawa, Atsushi; Lemoli, Roberto M; Dail, Monique; Duan, Yinghui; Chyla, Brenda; Potluri, Jalaja; Miller, Catherine L; Kantarjian, Hagop