Characterizing the Heme Uptake Proteins HtaB and ChtB in Corynebacterium diphtheriae

Corynebacterium diphtheriae is a Gram-positive, pathogenic bacterium. Pathogenic bacteria require iron as a key nutrient for survival. C. diphtheriae utilizes a direct heme uptake system in which the heme binds to a receptor protein that transfers the heme along the pathway to an ABC transporter, which facilitates the transfer of heme into the bacterial cell. This heme uptake pathway is encoded by the hmu and cht gene loci, which includes the genes for the surface-anchored proteins HtaB and ChtB. HtaB is proposed to have a function in transporting the heme obtained by HtaA to HmuT. ChtB is proposed to have a similar function to that of HtaB. Bioinformatics analysis shows that both HtaB and ChtB contain conserved tyrosine and histidine residues that are consistent with that of HtaA domains. A combination of UV-visible, circular dichroism, resonance Raman, and fluorescence spectroscopy have been used to characterize these proteins further

Investigating Three Approaches of Using Personality to Predict Competency-Based Performance

Organizations often use competencies to drive human capital initiatives such as recruitment, selection, training, and promotion. To serve such organizations, practitioners now offer various competency-based research solutions incorporating personality assessments to predict these competencies. Each approach begins by mapping competencies from an organization’s model to scientific models backed by synthetic and content validity evidence to align personality dimensions with each competency. This helps determine which personality dimensions drive performance for each competency. In this paper, we compare scale-based profiles, subscale-based algorithms, and scale-based algorithms to investigate the consistency of scores across methods and how effective each method is in predicting competency-based performance

Analyzing the Human Sex Ratio at Birth

The effect of income, education, employment, marital status, age, race, birth order, and national economic conditions on the sex ratio at birth were analyzed for the N = 21,597 children of the National Longitudinal Survey of Youth 1979 participants. These data were analyzed for individual births using a logistic regression model, treating the sex of each child as the outcome variable, and were analyzed for families using a linear regression model, treating the proportion of male children in each family as the outcome variable. No variable was statistically significantly related to the sex ratio. These findings suggest that the sex ratio at birth may not be affected by the individual- and population-level factors commonly examined in past research

Taiko: Its Past and Present in Japanese Society

The significance of Taiko in Japanese culture is far beyond being a percussion instrument. “Taiko” also refers to the art of drumming and is claimed to represent the spirit of Japanese people. Since ancient times, Taiko was used in the battlefield, Shinto and Buddhist religious ceremonies, festivals, royal courts, and theatrical arts performance. However, the origin of the Taiko remains unclear as well as its connection with Korean drum. The sacred power of Taiko for Japanese people is difficult to clarify in modern context. Japan is moving toward modernization, while promoting the importance of cultural preservation. It is important to understand the connection of the belief in Taiko that has been passed down since it first evidence appeared in the 6th century in Japan, from generation to generation, and how it survives in this modern Japanese society. To study the norm, performance practices, and its reception by musicians, religious leaders, and the public, an in-depth study of Taiko is necessary. The research, therefore aims to trace back its evolution, value, and its function in Japanese society. Results of the research display Taiko in relation to Japanese legend, development of Taiko, types of Taiko drum, and material used in Taiko manufacturing. More over, through research conducted, the connection of the past and present has provided Taiko with a new function in the modern society including of fusion music, contemporary music composition, and film music. Popular ensembles have brought Taiko to bridge the gap of traditional music of the past and Pop-Rock music of this modern world

Comparative Outcomes of Lung Volume Reduction Surgery and Lung Transplantation: A Systematic Review and Meta-Analysis

Background: Lung volume reduction (LVR) and lung transplantation (LTx) have been used in different populations of chronic obstructive pulmonary disease (COPD) patients. To date, comparative study of LVR and LTx has not been performed. We sought to address this gap by pooling the existing evidence in the literature. Methods: An electronic search was performed to identify all prospective studies on LVR and LTx published since 2000. Baseline characteristics, perioperative variables, and clinical outcomes were extracted and pooled for meta-analysis. Results: The analysis included 65 prospective studies comprising 3,671 patients [LTx: 15 studies (n=1,445), LVR: 50 studies (n=2,226)]. Mean age was 60 [95% confidence interval (CI): 58–62] years and comparable between the two groups. Females were 51% (95% CI: 30–71%) in the LTx group vs. 28% (95% CI: 21–36%) in LVR group (P=0.05). Baseline 6-minute walk test (6MWT) and pulmonary function tests were comparable except for the forced expiratory volume in 1 second (FEV1), which was lower in the LTx group [21.8% (95% CI: 16.8–26.7%) vs. 27.3% (95% CI: 25.5–29.2%), P=0.04]. Postoperatively, both groups experienced improved FEV1, however post-LTx FEV1 was significantly higher than post-LVR FEV1 [54.9% (95% CI: 41.4–68.4%) vs. 32.5% (95% CI: 30.1–34.8%), P\u3c0.01]. 6MWT was also improved after both procedures [LTx: 212.9 (95% CI: 119.0–306.9) to 454.4 m (95% CI: 334.7–574.2), P\u3c0.01; LVR: 286 (95% CI: 270.2–301.9) to 409.1 m (95% CI: 392.1–426.0), P\u3c0.01], however, with no significant difference between the groups. Pooled survival over time showed no significant difference between the groups. Conclusions: LTx results in better FEV1 but otherwise has comparable outcomes to LVR

Endovascular Intervention for Tracheo-Innominate Fistula: A Systematic Review and Meta-analysis

Introduction: Fistula formation between the trachea and the innominate artery is a life-threatening complication rarely seen with existing or previous tracheostomy. Fatal upon rupture, swift diagnosis and immediate intervention are paramount for survival. We aim to identify feasibility and outcomes of endovascular intervention for trachea-innominate fistula (TIF). Methods: Patient-level data of reported individuals above the age of 14 that underwent endovascular intervention for TIF was extracted and analyzed. Identification of 25 patients from 27 studies was accomplished by electronic database search of Cochrane Central Register of Controlled Trials, Cumulative Index to Nursing and Allied Health Literature, Ovid Medline, and Scopus. Survival data was evaluated by Kaplan-Meier analysis. Results: Median patient age was 39.0 years [IQR 16.0, 47.5]. Median time to TIF presentation following tracheostomy was 2.2 months [0.5, 42.5]. 84.6% (22/27) exhibited tracheal hemorrhage at presentation. Covered stent graft placement was performed in 96.3% (26/27) and coil embolization in 3.8% (1/27). Repeat endovascular intervention was necessary in 18.5% (5/27) and rescue sternotomy was required in 11.1% (3/27). Overall mortality was 29.6% (8/27) with a median follow-up time of 5 months [1.2, 11.5]. Discussion: Endovascular intervention may be an effective method of TIF repair at presentation. As an alternative to conventional surgical repair, endovascular intervention may be an appropriate method for TIF repair particularly in patients unfit for open sternotomy repair

Surgical ventricular reconstruction for ischemic cardiomyopathy-a systematic review and meta-analysis of 7,685 patients

Background: Surgical ventricular reconstruction (SVR) has been used to control adverse ventricular remodeling and improve cardiac function in ischemic cardiomyopathy. The purpose of this systematic review and meta-analysis was to collect and analyze all available evidence on the utilization and efficacy of SVR. Methods: An electronic database search was performed to identify all retrospective and prospective studies on SVR for ischemic cardiomyopathy in the English literature from 2000 through 2020. A total of 92 articles with a collective 7,685 patients undergoing SVR were included in the final analysis. Results: The mean patient age was 61 years (95% CI: 59-63) and 80% (78-82%) were male. Congestive heart failure was present in 66% (54-78%) and angina in 58% (45-70%). Concomitant coronary artery bypass grafting was undertaken in 92% (90-93%) while 21% (18-24%) underwent mitral valve repair. Pre vs. post-SVR, significant improvement was seen in left ventricular ejection fraction (LVEF) [29.9% (28.8-31.2%) vs. 40.9% (39.4-42.4%), P\u3c0.01], left ventricular end-systolic (LVESD) and end-diastolic diameters (LVEDD) [LVESD: 49.9 mm (48.1-51.7) vs. 45 mm (42.8-47.3), P\u3c0.01, LVEDD: 63.8 mm (62-65.6) vs. 58.23 mm (56.6-60), P\u3c0.01], and left ventricular end-systolic (LVESVI) and end-diastolic volume indices (LVEDVI) [LVESVI: 83.9 mL/m2 (79.3-88.4) vs. 46.8 mL/m2 (43.5-50.1), P\u3c0.01; LVEDVI: 119.9 mL/m2 (112.1-127.6) vs. 79.6 mL/m2 (73.6-85.7), P\u3c0.01]. Mean New York Heart Association class improved from 3 (2.8-3.1) to 1.8 (1.5-2) (P\u3c0.01). The 30-day mortality was 4% (3-5%) while late mortality was 19% (9-34%) at a mean follow-up of 27.5 [21-34] months. Conclusions: In patients with ischemic cardiomyopathy, SVR reduces left ventricular volumes and improves systolic function leading to symptomatic improvement

TGF-β blockade drives a transitional effector phenotype in T cells reversing SIV latency and decreasing SIV reservoirs in vivo

Abstract HIV-1 persistence during ART is due to the establishment of long-lived viral reservoirs in resting immune cells. Using an NHP model of barcoded SIVmac239 intravenous infection and therapeutic dosing of anti-TGFBR1 inhibitor galunisertib (LY2157299), we confirm the latency reversal properties of in vivo TGF-β blockade, decrease viral reservoirs and stimulate immune responses. Treatment of eight female, SIV-infected macaques on ART with four 2-weeks cycles of galunisertib leads to viral reactivation as indicated by plasma viral load and immunoPET/CT with a 64Cu-DOTA-F(ab’)2-p7D3-probe. Post-galunisertib, lymph nodes, gut and PBMC exhibit lower cell-associated (CA-)SIV DNA and lower intact pro-virus (PBMC). Galunisertib does not lead to systemic increase in inflammatory cytokines. High-dimensional cytometry, bulk, and single-cell (sc)RNAseq reveal a galunisertib-driven shift toward an effector phenotype in T and NK cells characterized by a progressive downregulation in TCF1. In summary, we demonstrate that galunisertib, a clinical stage TGF-β inhibitor, reverses SIV latency and decreases SIV reservoirs by driving T cells toward an effector phenotype, enhancing immune responses in vivo in absence of toxicity