A survey of genes encoding H2O2-producing GMC oxidoreductases in 10 Polyporales genomes

The genomes of three representative Polyporales (Bjerkandera adusta, Phlebia brevispora and a member of the Ganoderma lucidum complex) recently were sequenced to expand our knowledge on the diversity and distribution of genes involved in degradation of plant polymers in this Basidiomycota order, which includes most wood-rotting fungi. Oxidases, including members of the glucose-methanol-choline (GMC) oxidoreductase superfamily, play a central role in the above degradative process because they generate extracellular H2O2 acting as the ultimate oxidizer in both white-rot and brown-rot decay. The survey was completed by analyzing the GMC genes in the available genomes of seven more species to cover the four Polyporales clades. First, an in silico search for sequences encoding members of the aryl-alcohol oxidase, glucose oxidase, methanol oxidase, pyranose oxidase, cellobiose dehydrogenase and pyranose dehydrogenase families was performed. The curated sequences were subjected to an analysis of their evolutionary relationships, followed by estimation of gene duplication/ reduction history during fungal evolution. Second, the molecular structures of the near one hundred GMC oxidoreductases identified were modeled to gain insight into their structural variation and expected catalytic properties. In contrast to ligninolytic peroxidases, whose genes are present in all white-rot Polyporales genomes and absent from those of brown-rot species, the H2O2-generating oxidases are widely distributed in both fungal types. This indicates that the GMC oxidases provide H2O2 for both ligninolytic peroxidase activity (in white-rot decay) and Fenton attack on cellulose (in brown-rot decay), after the transition between both decay patterns in Polyporales occurred

Exploiting the Use of Convolutional Neural Networks for Localization in Indoor Environments

Indoor localization has been an active research area for the last two decades. A great number of sensors have been applied in the task of localization—some with high computational and energy demands (e.g. laser beams), or with issues related to the coverage area, for example, by making use of images obtained by a network of cameras. A different approach, which presents less energy demands and a wide area of coverage, can be created by means of the signal strength of wireless networks. The open issue with signal strength is its high instability due to interferences, attenuation and fading, which, in general, makes the localization systems to present less than desired accuracy. In this article, we exploit the use of Convolutional Neural Networks (ConvNets) in the task of localization. The main motivation behind the employment of ConvNets is its inherent ability of feature extraction, which we believe can deal better with the noise without a filtering step. We evaluate how ConvNets can be employed and identify the best topologies that lead to the lowest errors

Pelvic Girdle Pain in the Antepartum Population: Physical Therapy Clinical Practice Guidelines Linked to the International Classification of Functioning, Disability, and Health from the Section on Women’s Health and the Orthopaedic Section of the American Physical Therapy Association

The purposes of these clinical guidelines are to: Describe evidence-based physical therapy practice including diagnosis, prognosis, intervention, and assessment of outcome for musculoskeletal disorders commonly managed by women’s health and/or orthopedic physical therapists Classify and define common musculoskeletal conditions using the World Health Organization’s terminology related to impairments of body function and body structure, activity limitations, and participation restrictions Identify interventions supported by current best evidence to address impairments of body function and structure, activity limitations, and participation restrictions associated with common musculoskeletal conditions Identify appropriate outcome measures to assess changes resulting from physical therapy interventions Provide a description to policy makers, using internationally accepted terminology, of the practice of women’s health and/or orthopaedic physical therapists Provide information for payers and claims reviewers regarding the practice of women’s health and/or orthopaedic physical therapy for common musculoskeletal conditions Create a reference publication for women’s health and/or orthopedic physical therapy clinicians, academic instructors, clinical instructors, students, interns, residents, and fellows regarding the best current practice regarding women’s health and/or orthopaedic physical therap

Antihyperlipemic and antihypertensive effects of Spirulina maxima in an open sample of mexican population: a preliminary report

<p>Abstract</p> <p>Background</p> <p><it>Spirulina maxima </it>is a filamentous cyanobacterium used as food supplement because of its high nutrient contents. It has been experimentally proven, <it>in vivo </it>and <it>in vitro </it>that posses several pharmacological properties. The purpose of this study was to evaluate the effects of <it>Spirulina maxima </it>orally supplied (4.5 g/day, for 6 weeks) to a sample of 36 subjects (16 men and 20 women, with ages between 18–65 years) on serum lipids, glucose, aminotransferases and on blood pressure. The volunteers did not modify their dietary habits or lifestyle during the whole experimental period. From each subject, a sample of blood was drawn in fasting state of 12 hours to determi the plasma concentrations of glucose, triacylglycerols (TAG), total cholesterol (TC), cholesterol associated to high density lipoprotein (HDL-C) and aspartate aminotransferase (AST). Anthropometric measurements including systolic (SYST-P) and diastolic (DIAST-P) blood pressure, height, weight and Body Mass Index (BMI) were also recorded.</p> <p>Results</p> <p>Comparing initial and final data, the results showed that there were no significant changes in the values of glucose and AST, but significant differences in TAG, TC, and HDL-C, were observed: TAG 233.7 ± 177.8 vs. 167.7 ± 100.7 mg/dL (p < 0.001), TC 181.7 ± 37.5 vs. 163.5 ± 34.4 mg/dL (p < 0.001), C-HDL 43.5 ± 14.4 vs. 50 ± 18.8 mg/dL (p < 0.01). The univariated analysis showed that the changes in the HDL-C and TC concentrations were dependent on TAG concentration (p = 0.247 and p = 0.108, respectively); nevertheless the calculated values for cholesterol associated to low density lipoprotein (LDL-C) were significantly reduced by the <it>Spirulina maxima </it>treatment but independently of the TAG changes. In addition, significant differences were found comparing initial and final SYST-P and DIAST-P blood pressure in both male and female: SYST-P male 121 ± 9 vs. 111 ± 8 mm Hg (p < 0.01), DIAST-P male 85 ± 6.5 vs. 77 ± 9 mm Hg (p < 0.01); SYST-P female 120 ± 9.5 vs. 109 ± 11 mm Hg (p < 0.002), DIAST-P female 85 ± 11 vs. 79 ± 7.5 mm Hg (p < 0.03).</p> <p>Conclusion</p> <p>The <it>Spirulina maxima </it>showed a hypolipemic effect, especially on the TAG and the LDL-C concentrations but indirectly on TC and HDL-C values. It also reduces systolic and diastolic blood pressure.</p

Green chemistry in Brazil

The philosophy of green chemistry has been very well received in Latin America's research and development programs. In this review we describe the green chemistry contributions of Brazilian research groups over the last three years.Centro de Investigación y Desarrollo en Ciencias Aplicada

Green chemistry in Brazil

The philosophy of green chemistry has been very well received in Latin America's research and development programs. In this review we describe the green chemistry contributions of Brazilian research groups over the last three years.Centro de Investigación y Desarrollo en Ciencias Aplicada

Seeing Andalucia’s Late Gothic heritage through GIS and Graphs

This study discusses the methodology used in The Digital model of Andalusia's Late Gothic Heritage project to develop new models of heritage interpretation through the application of GIS and Graph visualization to provide new perspectives of Andalusia's heritage by considering social, political, economic and cultural evolutions. Given the special period context considered, late 15th and early 16th centuries, the project used a variety of sources to relate heterogeneous historical data on different subjects in order to create a historical spatial database and to respond questions such as: How was the construction process in Andalusia between 1433 and 1560? What period and region had more constructive activity? How did the opening of the Andalusia´s eastern border modify the dynamism of the territory? What features are more common in each time period? What professionals have worked together on a building or quarry? Is this reflected in the architectural language produced?

Floral parts of Gomphrena globosa L. as a novel alternative source of betacyanins: Optimization of the extraction using response surface methodology

The present study describes a novel mechanical process for the pigmented parts of Gomphrena globosa L. The effects of the variables of the maceration extraction of betacyanins have not been properly described. Therefore, this study also aims to optimize the conditions that maximize betacyanins extraction from G. globosa as an alternative source. Assisted by response surface methodology, an experimental design was developed for testing the extraction variables (time, temperature, ethanol-water proportion and solid-liquid ratio). The responses used were betacyanins quantification (by HPLC-PDA-MS/ESI and spectrophotometric analysis), the extraction-yield and the colour intensity of the produced powder. The betacyanins identified were gomphrenin and isogomphrenin II and III. The highest betacyanins content ( 45 mg/g) was obtained by 165 min, 25 C, 0% of ethanol and 5 g/L of solid-liquid ratio. The betacyanins content from the floral parts of G. globosa is higher than those normally found in other sources highlighting its industrial application.The authors thank the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013) and L. Barros (SFRH/ BPD/107855/2015) grant. To POCI-01-0145-FEDER-006984 (LA LSRE-LCM) funded by ERDF through POCI-COMPETE2020 and FCT. To Xunta de Galicia for financial support for the postdoctoral researcher of M.A. Prieto. The authors also thank Ana Maria Carvalho for the plant donation and Celestino Santos- Buelga for the preparative LC system utilisation

Comparison of different bread types: chemical and physical parameters

In this work, the chemical and physical profile of 5 different bread types (Multicereal bread, Bavaria wheat bread, Wholemeal bread, Rye and Oat bread) were analysed in depth, namely the nutritional profile, individual fatty acids and soluble sugars through GC-FID and HPLC-RI, respectively, as well as the mineral profile, including micro and macroelements. Furthermore, a texture profile analysis was carried out in addition to the measurement of the crust colour. Each bread type showed a distinct profile, with Wholemeal and Bavaria having the lowest calories, and Oat the highest. Multicereal showed the highest amount of unsaturated fatty acids, while Wholemeal and Rye scored the least sodium amounts. The hardest bread was Rye and the easiest to chew were Oat and Bavaria breads. The latter was also the one with the darkest crumb of all the analysed breads. This work shows that bread can be baked to meet the needs and particularities of various kinds of diets.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER, under Program PT2020 for financial support to CIMO (UID/AGR/00690/2019). This work was funded by the European Structural and Investment Funds (FEEI) through the Regional Operational Program North 2020, within the scope of Project Mobilizador ValorNatural®, and funded by ALIMNOVA research group (UCM 252/2017). The authors are also grateful to FEDER-Interreg España-Portugal programme for financial support through the project TRANSCoLAB 0612_TRANS_CO_LAB_2_P. L. Barros and S. Heleno thank the National funding by FCT, P.I., through the institutional scientific employment and individual program-contract, respectively. M. Carocho thanks ValorNatural® project for his contract. The authors also thank M. Ferreira & Filhas (Pão de Gimonde) for providing the bread samples used in this work.info:eu-repo/semantics/publishedVersio

Modern extraction techniques optimized to extract betacyanins from Gomphrena globosa L.

Gomphrena globosa L. is a source of betacyanins, molecules with high colorant power. In order to replace conventional extraction techniques, microwave (MAE) and ultrasound assisted extraction (UAE) processes were optimized by the response surface methodology (RSM) to maximize the recovery of betacyanins from G. globosa. A five-level full factorial design of 24 combinations and 4 replicates was implemented for MAE and UAE. The optimum processing conditions for MAE (t = 8 min; T = 60 °C; Et = 0%; and S/L = 5 g/L) conducted to an extraction yield of 39.6 ± 1.8 mg/g dry weight, while for UAE (t = 22 min; P = 500%; Et = 0%; and S/L = 5 g/L) a value of 46.9 ± 4.8 mg/g was achieved, validating the UAE more suitable to obtain the target compounds. This technique can provide extracts rich in betacyanins and with high potential to be used as natural colorants.The authors thank the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013) and L. Barros (SFRH/BPD/107855/2015) and C.L. Roriz (SFRH/BD/117995/2016) grants. This work was also financially supported by: Project POCI-01-0145-FEDER-006984 – Associate Laboratory LSRE-LCM funded by FEDER through COMPETE2020 – Programa Operacional Competitividade e Internacionalização (POCI) and by national funds through FCT – Fundação para a Ciência e a Tecnologia. To Xunta de Galicia for financial support for the post-doctoral researcher of M.A. Prieto. The authors also thank Ana Maria Carvalho for the plant donation.info:eu-repo/semantics/publishedVersio