Cytokine properties of CD23 on human Eosinophilic cells

CD23, the low affinity IgE receptor, is expressed by various cell types and has numerous functions depending on the form of the protein, its interaction with various ligands and the type of cell involved. CD23 is pivotal in the regulation of IgE, with the soluble form involved in up-regulation, while the membrane bound form is involved in the down-regulation. It is clear why it is believed to be a central molecule in allergic responses, and a therapeutic target for the treatment of allergic disease. In this study a recombinant form of the entire extracellular domain of the protein, exCD23, was produced by PCR cloning and expressed in E. coli. His•Tag™s were introduced onto the C-terminus and N-terminus, respectively, in order to simplify the purification procedure. After renaturation and purification, the recombinant exCD23 bound IgE, indicating its activity. From the IgE binding studies it was established that the position of the tag did not influence the binding. GST•Tagged™ exCD23 was also produced in an attempt to increase the solubility of the recombinant protein, but this proved unsuccessful. Butyrate differentiated EoL-1 cells were treated with the Nterminal His•Tagged™ exCD23, and the protein appeared to suppress the secretion of the constitutively expressed cytokines, especially IL-8 and IFN- , when compared to untreated cells. In addition, treatment of the EoL-1 cells with exCD23 had a significant proliferative effect, but could not induce differentiation of this cell line into mature eosinophilic-like cells

Brief Report on Transgender Students with Disabilities: Best Practices for Higher Education

Transgender is an umbrella term that includes individuals whose sense of gender identity does not match the sex assigned at birth. Transgender students with physical or psychiatric disabilities face additional barriers on college campuses due to the compounded effects of prejudice towards transgender identity (transphobia) and prejudice towards disability (ableism). Transgender individuals with disabilities often experience double stigma that may involve institutional barriers such as unemployment, educational discrimination, or residential segregation (Mizock & Lewis, 2008). In addition, transgender individuals with disabilities report ableism, such as being stared at, avoided, infantilized, harassed, threatened, or ignored (Clare, 2001). Experiences of mistreatment may vary depending on the degree to which a student’s transgender identity, or physical or psychiatric disability, is visibly discernible to others. The literature has generally overlooked the unique needs of transgender students with disabilities. When transgender individuals are mentioned in the literature, they are often lumped within the broader grouping of lesbian, gay, and bisexual students (together, LGBT) despite the differences between issues of sexual identity and those of gender identity (Mizock & Fleming, 2011). Higher education faculty and staff can develop awareness of the prejudice and discrimination that transgender students with disabilities face in order to provide support and raise awareness of the needs of this population on campus. Best practices for inclusion will be presented in this brief report to assist college professionals in addressing the needs of transgender students with psychiatric and physical disabilities

Parents’ Perceptions Of Safety In Public Space And Adolescent Well-Being In Ethiopia, Peru, And Vietnam

Safety in public space is a critical concern, particularly for women and girls, and these concerns may have consequences for well-being. Most scholarship to date, however, is cross-sectional; little is known about the longer-term impact of perceiving public space as unsafe. Among adolescents, the relationship between safety and well-being is likely influenced by parents. This study used longitudinal analysis to examine the factors that contribute to parents’ perceptions of adolescent safety at age 15, the relationship between these perceptions and adolescent well-being at age 19, and the differences for boys and girls. Data were drawn from Young Lives, a multi-country panel study. The sample included 820 parent/adolescent dyads in Ethiopia, 620 in Peru, and 941 in Vietnam. Descriptive statistics and multivariate regressions were conducted. Perceiving one’s child unsafe in public space was highest in Peru (two in three parents), followed Vietnam (one in three parents), and Ethiopia (one in five parents). In the adjusted analyses, there were two significant findings. In Ethiopia and Peru, girls were more likely than boys to be perceived as unsafe. Adolescents in certain regions of Ethiopia and Vietnam also were more likely to be perceived as unsafe. No associations were detected between parents’ perceptions of adolescent safety at age 15 and adolescent well-being at age 19. Parents’ concerns for adolescent safety are substantial, especially in Peru. Girls’ safety is of particular concern and deserves more public health attention. The regional variation in parents’ perceptions suggests that it is a local phenomenon and requires locally-driven intervention. Although no association between parents’ perceptions of safety and adolescent well-being was found, prior research supports this link. Young Lives provided one of the few data sets equipped to examine this relationship longitudinally, however, it had limitations – offering just a single item measure for safety concerns. Better data is needed. This investigation lays the groundwork for subsequent research, which is needed, and should: utilize a robust measure of perceptions of safety; consider the importance of other community factors (e.g., rates of violence); and test additional measures of well-being; physical and mental health would offer important contributions to the field

Parents’ Perceptions Of Safety In Public Space And Adolescent Well-Being In Ethiopia, Peru, And Vietnam

Safety in public space is a critical concern, particularly for women and girls, and these concerns may have consequences for well-being. Most scholarship to date, however, is cross-sectional; little is known about the longer-term impact of perceiving public space as unsafe. Among adolescents, the relationship between safety and well-being is likely influenced by parents. This study used longitudinal analysis to examine the factors that contribute to parents’ perceptions of adolescent safety at age 15, the relationship between these perceptions and adolescent well-being at age 19, and the differences for boys and girls. Data were drawn from Young Lives, a multi-country panel study. The sample included 820 parent/adolescent dyads in Ethiopia, 620 in Peru, and 941 in Vietnam. Descriptive statistics and multivariate regressions were conducted. Perceiving one’s child unsafe in public space was highest in Peru (two in three parents), followed Vietnam (one in three parents), and Ethiopia (one in five parents). In the adjusted analyses, there were two significant findings. In Ethiopia and Peru, girls were more likely than boys to be perceived as unsafe. Adolescents in certain regions of Ethiopia and Vietnam also were more likely to be perceived as unsafe. No associations were detected between parents’ perceptions of adolescent safety at age 15 and adolescent well-being at age 19. Parents’ concerns for adolescent safety are substantial, especially in Peru. Girls’ safety is of particular concern and deserves more public health attention. The regional variation in parents’ perceptions suggests that it is a local phenomenon and requires locally-driven intervention. Although no association between parents’ perceptions of safety and adolescent well-being was found, prior research supports this link. Young Lives provided one of the few data sets equipped to examine this relationship longitudinally, however, it had limitations – offering just a single item measure for safety concerns. Better data is needed. This investigation lays the groundwork for subsequent research, which is needed, and should: utilize a robust measure of perceptions of safety; consider the importance of other community factors (e.g., rates of violence); and test additional measures of well-being; physical and mental health would offer important contributions to the field

Parents’ Perceptions Of Safety In Public Space And Adolescent Well-Being In Ethiopia, Peru, And Vietnam

Safety in public space is a critical concern, particularly for women and girls, and these concerns may have consequences for well-being. Most scholarship to date, however, is cross-sectional; little is known about the longer-term impact of perceiving public space as unsafe. Among adolescents, the relationship between safety and well-being is likely influenced by parents. This study used longitudinal analysis to examine the factors that contribute to parents’ perceptions of adolescent safety at age 15, the relationship between these perceptions and adolescent well-being at age 19, and the differences for boys and girls. Data were drawn from Young Lives, a multi-country panel study. The sample included 820 parent/adolescent dyads in Ethiopia, 620 in Peru, and 941 in Vietnam. Descriptive statistics and multivariate regressions were conducted. Perceiving one’s child unsafe in public space was highest in Peru (two in three parents), followed Vietnam (one in three parents), and Ethiopia (one in five parents). In the adjusted analyses, there were two significant findings. In Ethiopia and Peru, girls were more likely than boys to be perceived as unsafe. Adolescents in certain regions of Ethiopia and Vietnam also were more likely to be perceived as unsafe. No associations were detected between parents’ perceptions of adolescent safety at age 15 and adolescent well-being at age 19. Parents’ concerns for adolescent safety are substantial, especially in Peru. Girls’ safety is of particular concern and deserves more public health attention. The regional variation in parents’ perceptions suggests that it is a local phenomenon and requires locally-driven intervention. Although no association between parents’ perceptions of safety and adolescent well-being was found, prior research supports this link. Young Lives provided one of the few data sets equipped to examine this relationship longitudinally, however, it had limitations – offering just a single item measure for safety concerns. Better data is needed. This investigation lays the groundwork for subsequent research, which is needed, and should: utilize a robust measure of perceptions of safety; consider the importance of other community factors (e.g., rates of violence); and test additional measures of well-being; physical and mental health would offer important contributions to the field

Genotype and sex-based host variation in behavior and susceptibility drives population disease dynamics

Host heterogeneity in pathogen transmission is widespread and presents a major hurdle to predicting and minimizing disease outbreaks. Using Drosophila melanogaster infected with Drosophila C virus as a model system, we integrated experimental measurements of social aggregation, virus shedding, and disease-induced mortality from different genetic lines and sexes into a disease modelling framework. The experimentally measured host heterogeneity produced substantial differences in simulated disease outbreaks, providing evidence for genetic and sex-specific effects on disease dynamics at a population level. While this was true for homogeneous populations of single sex/genetic line, the genetic background or sex of the index case did not alter outbreak dynamics in simulated, heterogeneous populations. Finally, to explore the relative effects of social aggregation, viral shedding and mortality, we compared simulations where we allowed these traits to vary, as measured experimentally, to simulations where we constrained variation in these traits to the population mean. In this context, variation in infectiousness, followed by social aggregation, was the most influential component of transmission. Overall, we show that host heterogeneity in three host traits dramatically affects population-level transmission, but the relative impact of this variation depends on both the susceptible population diversity and the distribution of population-level variation

DISTÚRBIOS PSÍQUICOS MENORES EM TRABALHADORES DE ENFERMAGEM DE UM BLOCO CIRÚRGICO

Objetivo: analisar a proporção e os fatores associados aos Distúrbios Psíquicos Menores em trabalhadores de enfermagem de um Bloco Cirúrgico. Método: estudo descritivo, exploratório, de abordagem quantitativa, realizado com 54 trabalhadores de enfermagem. Para a coleta de dados, utilizou-se questionário estruturado contendo características sociodemográficas e ocupacionais, a versão brasileira do Self Reporting Questionnaire-20 e o Demand Control Support Questionnaire. Resultados: a proporção de Distúrbios Psíquicos Menores foi de 24,1%. Em relação aos aspectos psicossociais, foram predominantemente classificados como desenvolvendo um trabalho com baixa demanda psicológica (63,0%), baixo controle (70,4%) e baixo apoio social (72,2%). Das variáveis investigadas, apresentaram associação estatisticamente significativa com o desfecho o sexo feminino e o controle no trabalho. Conclusão: os resultados evidenciaram a proporção de 24,1% para os Distúrbios Psíquicos Menores nos trabalhadores de enfermagem do local estudado; quanto aos fatores associados, o sexo feminino e o controle no trabalho apresentaram associação significativa com o desfecho.Descritores: Centro Cirúrgico. Enfermagem. Saúde do Trabalhador. Trabalhadores. Transtornos Mentais

Soccer players in sports television journalism: an analysis of the typifications in the series “Players of the Brazilian national soccer team” in Jornal Nacional

O artigo analisa as tipificações dos jogadores de futebol construídas na série especial “Jogadores da Seleção” veiculada pelo Jornal Nacional antes da Copa do Mundo de 2014. Com base em Williams (1979), a tipificação é entendida como uma figura específica que concentra e intensifica uma realidade mais geral e complexa. A partir da metodologia da análise textual (Casetti e Chio, 1999), mapeamos as tipificações construídas na série especial do telejornal a partir de duas categorias analíticas: a) sujeitos e interações e b) história. Para a análise, foram selecionadas três reportagens relacionadas às histórias de vida de Daniel Alves, Victor e Maxwell. Como resultados, chegamos a três tipificações de jogadores de futebol na série: o tipo pobre, caracterizado como hegemônico e apresentado de forma destacada na série; o tipo graduado, representado unicamente pelo jogador Victor por meio de uma concessão e, por fim, o tipo rico, exemplificado exclusivamente pelo jogador Maxwell, que aparece de forma atenuada. O estudo revela que a série concede um falso protagonismo aos jogadores de futebol, uma vez que estes são representados de forma passiva, segundo os interesses mercadológicos e ideológicos da emissora. Assim, observamos que a série não problematiza a pluralidade das histórias de vida dos jogadores de futebol, uma vez que apenas um único tipo hegemônico aparece de forma destacada no telejornal.Palavras-chave: Tipificação. Telejornalismo esportivo. Jogadores de futebol.The paper analyzes the typifications of soccer players in the special series “Players of the Brazilian national soccer team” broadcasted by Jornal Nacional before the 2014 World Cup. Based on Williams (1979), typification is understood as a specific figure that concentrates and intensifies a more general and complex reality. Based on the methodology of textual analysis (Casetti and Chio, 1999), we mapped the typifications in the special series of the TV newscast from two analytical categories: a) subjects and interactions, and b) history. For the analysis, three reports related to the life stories of Daniel Alves, Victor and Maxwell were selected. As results, we pointed out three typifications of soccer players in the series: the poor type, characterized as hegemonic and featured prominently in the series; the graduated type, represented only by the player Victor by means of a concession, and finally the rich type, exclusively exemplified by the player Maxwell, who appears attenuated. The study reveals that the series gives a false protagonism to the soccer players, since they are represented in a passive way, according to the economic and ideological interests of the TV channel. Thus, we observe that the series does not problematize the plurality of the soccer players’ life stories, since only a single hegemonic type appears prominently in the TV newscast.Keywords: Typification. Sports television journalism. Soccer players

Estruturas de sentimento e formações discursivas: aproximações possíveis

O artigo propõe-se a reconhecer os pontos de aproximação entre os conceitos de estruturas de sentimento e formações discursivas à luz das perspectivas dos Estudos Culturais e da Análise de Discurso. Como resultado, tem-se a necessidade de aprofundar os conceitos dos Estudos Culturais e da Análise de Discurso a fim de compreender como os significados são produzidos discursivamente através da linguagem a partir do repertório cultural dos sujeitos.   

Análise cultural das representações dos impactos das Olimpíadas Rio 2016 no The Guardian

O artigo analisa as representações dos impactos das Olimpíadas Rio 2016 construídas na série “Vozes do Rio: nossa odisseia olímpica”, publicada no portal de notícias do jornal The Guardian de 05 de agosto de 2015 a 19 de agosto de 2017. A série é composta por 21 relatos escritos por jornalistas comunitários de três favelas cariocas sobre as repercussões das Olimpíadas Rio 2016 no seu dia a dia. Como base teórico-metodológica, recorremos à análise cultural, concebida na ótica dos Estudos Culturais, para discutir as mediações entre a cultura vivida nas favelas cariocas e a construção de representações no discurso jornalístico. Como resultado, concluímos que o megaevento esportivo foi representado como um período de silenciamento das margens, levando à intensificação do processo de periferização das minorias sociais no país. Pontuamos, por fim, as contribuições do acionamento das vozes marginalizadas na série para a aproximação entre a cultura vivida e o discurso jornalístico