Effects of culture media and suspension expansion technologies in mesenchymal stem cell manufacturing - A computational bioprocess and bioeconomics study

Mesenchymal stem cell (MSC) based therapies are promising for a large spectrum of unmet medical needs. Despite this promise, the scaling-up of production of clinical grade MSCs is hindered by the use of planar technologies that require intensive labor and are not enough to meet market demands, as well as due to high product and process variability introduced by the use of xenogeneic materials. This work presents a new bioprocess and bioeconomics model of stem cell expansion to support informed decisions for stem cells process scaling up at reduced annual costs. The intrinsic equations and parameters that capture the cell biological features, according with their source and media used, are embedded in the model. A target number of cells per dose of 140 million and a GMP facility of 400 sq mt with 4 BSCs and 8 incubators will be used as the baseline for expansion of both bone marrow MSCs (BM-MSCs) and adipose stem cells (ASCs) using planar expansion technologies. The current standard medium for MSC culture containing fetal bovine serum (FBS) will be compared with the xeno-free alternative of human platelet lysate (hPL). The use of hPL for both cell sources results in an increase of the number of doses produced and a decrease of the cost of goods (CoG) per dose (Table 1). In order to improve the production capacity, 8 bioreactors with capacity up to 50L were input in the model, using xeno-free plastic microcarriers for cell adhesion and hPL as the culture medium. The model results indicate that the investment in the use of suspension cultures is valuable due to a considerable increase in the production and a decrease of CoG/dose. As the number of doses produced per year increases, the reagent costs dominate relatively to the facility costs (Fig. 1). Sensitivity analysis was performed by varying 11 model variables by +/- 33%. The main factors that influence annual capacity and CoGs are related to harvesting density and yield, growth rates and microcarrier area and concentration (Table 2). These findings may be used to improve the design of expansion methods with fully xeno-free materials and highlight the relevance of the optimization of harvesting and downstream processing protocols. Please click Additional Files below to see the full abstract

Simulation of a robotic bird

In this paper it is studied the dynamics of the robotic bird. The system performances are analyzed in terms of time response and robustness. It is study the relation between the angle of attack and the velocity of the bird, the tail influence, the gliding flight and the flapping flight. In this model, a bird flies by the wind beat motion or using its tail down. The results are positive for the construction of flying robots. The development of computational simulation based on the dynamic of the robotic bird that should allow testing strategies and algorithms of control.N/

The active mathematics project at ISCAP

This paper summarizes a project that is contributing to a change in the way of teaching and learning Mathematics. Mathematics is a subject of the Accounting and Administration course. In this subject we teach: Functions and Algebra. The aim is that the students understand the basic concepts and is able to apply them in other issues, when possible, establishing a bridge between the issues that they have studied and their application in Accounting. As from this year, the Accounting course falls under in Bologna Process. The teacher and the student roles have changed. The time for theoretical and practical classes has been reduced, so it was necessary to modify the way of teaching and learning. In the theoretical classes we use systems of multimedia projection to present the concepts, and in the practical classes we solve exercises. To supplement our theoretical and practical classes we have developed an active mathematics project called MatActiva based on the Moodle platform offered by PAOL - Projecto de Apoio Online (Online Support Project). In the last versions of Moodle, it is possible use the TeX language to create math questions. Using this tool we created a set of interactive materials. With the creation of this new project we wanted to take advantage already obtained results with the previous experiences, giving to the students opportunities to complement their study in Mathematics. One of the great objectives is to motivate students, encourage them to overcome theirs difficulties through an auto-study, giving them more confidence and the opportunity to seeing others perspectives of the mathematics subjects. In the MatActiva project the students have a big collection of information about the way of the subject works, which includes the objectives, the program, recommended bibliography, evaluation method and summaries. It works as material support for the practical and theoretical classes, the slides of the theoretical classes are available, the sheets with exercises for the students to do in the classroom and complementary exercises, as well as the exams of previous years. Students can also do diagnostic tests and evaluation tests online. Our approach is a reflexive one, based on the professional experience of the teachers that explore and incorporate new tools of Moodle with their students and coordinate the project MatActiva

Lattice three-gluon vertex in extended kinematics: Planar degeneracy

We present novel results for the three-gluon vertex, obtained from an extensive quenched lattice simulation in the Landau gauge. The simulation evaluates the transversely projected vertex, spanned on a special tensorial basis, whose form factors are naturally parametrized in terms of individually Bose-symmetric variables. Quite interestingly, when evaluated in these kinematics, the corresponding form factors depend almost exclusively on a single kinematic variable, formed by the sum of the squares of the three incoming four-momenta, q, r, and p. Thus, all configurations lying on a given plane in the coordinate system (q2,r2,p2) share, to a high degree of accuracy, the same form factors, a property that we denominate planar degeneracy. We have confirmed the validity of this property through an exhaustive study of the set of configurations satisfying the condition q2=r2, within the range [0,5GeV]. This drastic simplification allows for a remarkably compact description of the main bulk of the data, which is particularly suitable for future numerical applications. A semi-perturbative analysis reproduces the lattice findings rather accurately, once the inclusion of a gluon mass has cured all spurious divergences.The authors thank A. C. Aguilar, G. Eichmann and C. D. Roberts for useful discussions. M.N.F. acknowledges financial support from the FAPESP projects 2017/05685-2 and 2020/12795-1, respectively. J.P. is supported by the Spanish MICINN grant PID2020-113334GB-I00 and the regional Prometeo/2019/087 from the Generalitat Va-lenciana; while F.D.S. and J.R.Q. are by the Spanish MICINN grant PID2019-107844GB-C22 and the regional Andalusian P18-FR-5057. All calculations have been performed at the UPO computing center, C3UPO

Collapse of ρxx\rho_{xx} ringlike structures in 2DEGs under tilted magnetic fields

In the quantum Hall regime, the longitudinal resistivity $\rho_{xx}$ plotted as a density--magnetic-field ($n_{2D}-B$) diagram displays ringlike structures due to the crossings of two sets of spin split Landau levels from different subbands [e.g., Zhang \textit{et al.}, Phys. Rev. Lett. \textbf{95}, 216801 (2005)]. For tilted magnetic fields, some of these ringlike structures "shrink" as the tilt angle is increased and fully collapse at $\theta_c \approx 6^\circ$. Here we theoretically investigate the topology of these structures via a non-interacting model for the 2DEG. We account for the inter Landau-level coupling induced by the tilted magnetic field via perturbation theory. This coupling results in anti-crossings of Landau levels with parallel spins. With the new energy spectrum, we calculate the corresponding $n_{2D}-B$ diagram of the density of states (DOS) near the Fermi level. We argue that the DOS displays the same topology as $\rho_{xx}$ in the $n_{2D}-B$ diagram. For the ring with filling factor $\nu=4$, we find that the anti-crossings make it shrink for increasing tilt angles and collapse at a large enough angle. Using effective parameters to fit the $\theta = 0^\circ$ data, we find a collapsing angle $\theta_c \approx 3.6^\circ$. Despite this factor-of-two discrepancy with the experimental data, our model captures the essential mechanism underlying the ring collapse.Comment: 3 pages, 2 figures; Proceedings of the PASPS V Conference Held in August 2008 in Foz do Igua\c{c}u, Brazi

Fish larvae quality descriptors: an appraisal of methods for red porgy Pagrus pagrus and grouper Epinephelus marginatus produced under different rearing conditions

Quality control in fish hatcheries is of paramount importance to achieve the desired characteristics of larvae and fry, either for the market or release to the wild. Quality programmes are applied in a day to day basis having direct implications for production management and final costs of product. Most common criteria used for larval quality assessment are morphometrics, condition factor, histometrical indices, lipid analysis, nucleic acid ratios, enzyme activity and stress tests

Schwinger mechanism for gluons from lattice QCD

Continuum and lattice analyses have revealed the existence of a mass-scale in the gluon two-point Schwinger function. It has long been conjectured that this expresses the action of a Schwinger mechanism for gauge boson mass generation in quantum chromodynamics (QCD). For such to be true, it is necessary and sufficient that a dynamically-generated, massless, colour-carrying, scalar gluon+gluon correlation emerges as a feature of the dressed three-gluon vertex. Working with results on elementary Schwinger functions obtained via the numerical simulation of lattice-regularised QCD, we establish with an extremely high level of confidence that just such a feature appears; hence, confirm the conjectured origin of the gluon mass scale