FORMAÇÃO DO PREÇO DE VENDA: ESTUDO EM UMA EMPRESA DE TRANSPORTE RODOVIÁRIO DE CARGA

A estratégia na formação do preço de venda é relevante para a sobrevivência das organizações no mercado competitivo em que estão inseridas, embora no ramo de transporte a formação do preço de venda seja determinada pela concorrência. O estudo tem como objetivo principal identificar os custos operacionais envolvidos na formação de preço de venda em uma empresa de transporte rodoviário de carga. E ainda, como objetivos específicos: a) caracterizar a empresa objeto de estudo; b) estimar os custos logísticos e operacionais da empresa analisada; c) analisar os componentes da formação do preço de venda. Na metodologia, optou-se por um estudo de caso descritivo em uma empresa de transporte de carga com sede na cidade de Chapecó/SC e utilizou uma uma abordagem quantitativa dos dados. Os dados foram coletados durante o primeiro semestre de 2016 por meio de relatórios gerenciais e entrevistas com os gestores da organização. Os resultados evidenciam que a empresa forma seus preços com base no mercado, e não possui um método específico para a formação do seu preço de venda. A empresa não possui um centro de custos para identificar os componentes que influenciam na formação do preço e também não realiza a gestão de seus custos operacionais. Entretanto, a organização realização a presunção de receita líquida desejada. na ordem de 20% de acordo com as informações repassadas pelos gestores, porém o valor real do seu lucro está na ordem de 17,24%

Eficacia y costo-utilidad de primer reemplazo total de cadera y rodilla en pacientes con osteoartritis

Objetivo: determinar la eficacia, costos y razón incremental de costo-utilidad (RICU) de la cirugía de primer reemplazo total de rodilla y cadera en pacientes con osteoartritis

FORMAÇÃO DO PREÇO DE VENDA: ESTUDO EM UMA EMPRESA DE TRANSPORTE RODOVIÁRIO DE CARGA

A estratégia na formação do preço de venda é relevante para a sobrevivência das organizações no mercado competitivo em que estão inseridas, embora no ramo de transporte a formação do preço de venda seja determinada pela concorrência. O estudo tem como objetivo principal identificar os custos operacionais envolvidos na formação de preço de venda em uma empresa de transporte rodoviário de carga. E ainda, como objetivos específicos: a) caracterizar a empresa objeto de estudo; b) estimar os custos logísticos e operacionais da empresa analisada; c) analisar os componentes da formação do preço de venda. Na metodologia, optou-se por um estudo de caso descritivo em uma empresa de transporte de carga com sede na cidade de Chapecó/SC e utilizou uma uma abordagem quantitativa dos dados. Os dados foram coletados durante o primeiro semestre de 2016 por meio de relatórios gerenciais e entrevistas com os gestores da organização. Os resultados evidenciam que a empresa forma seus preços com base no mercado, e não possui um método específico para a formação do seu preço de venda. A empresa não possui um centro de custos para identificar os componentes que influenciam na formação do preço e também não realiza a gestão de seus custos operacionais. Entretanto, a organização realização a presunção de receita líquida desejada. na ordem de 20% de acordo com as informações repassadas pelos gestores, porém o valor real do seu lucro está na ordem de 17,24%

Facile and Mild Synthesis of Linear and Cyclic Peptides via Thioesters

Thioester-mediated peptide bond formation has recently garnered a lot of attention, most notably in its relevance to condensation of large peptide fragments. Herein, a simple and general ligation method for the preparation of linear and cyclic peptides, starting from peptide thioester, mainly <i>p</i>-chlorophenyl, precursors is reported. The inherent advantages of this method are the low epimerization, reduced dimerization, use of mild reaction conditions, and elimination of superfluous coupling reagents

Two novel DNA variants associated with glucose-6-phosphate dehydrogenase deficiency found in Argentine pediatric patients

Objective The enzyme glucose-6-phosphate dehydrogenase (G6PD) catalyses the first step in the pentose phosphate pathway, producing nicotinamide adenine dinucleotide phosphate (NADPH). NADPH plays a crucial role in preventing oxidative damage to proteins and other molecules in cells, mostly red blood cells. G6PD deficiency has an X-linked pattern of inheritance in which hemizygous males are deficient, while females may or may not be deficient depending on the number of affected alleles. We report two novel DNA variants in the G6PD gene detected in two male probands with chronic nonspherocytic hemolytic anemia (CNSHA), who were referred for hematological evaluation. Method Probands and their relatives underwent clinical, biochemical, and molecular assessment. Results Two novel DNA variants, c.995C > T and c.1226C > A, were found in this study. At the protein level, they produce the substitution of Ser332Phe and Pro409Gln, respectively. These DNA variants were analyzed in the female relatives of probands for genetic counseling. Conclusions The novel DNA variants were classified as class I based on the clinical, biochemical, and molecular evaluations performed.Fil: Chaves, Alejandro. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Eberle, Silvia Eandi. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Defelipe, Lucas Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Pepe, Carolina Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Milanesio, Berenice. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Aguirre, Fernando. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Fernandez, Diego. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Turjanski, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Feliú Torres, Aurora. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentin

Detection of minimally disseminated disease in the cerebrospinal fluid of children with high-risk retinoblastoma by reverse transcriptase-polymerase chain reaction for GD2 synthase mRNA

Aim: To evaluate minimally disseminated disease (MDD) in cytologically negative cerebrospinal fluid (CSF) specimens of patients with high-risk retinoblastoma by the detection of the synthase of ganglioside GD2 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR). Methods: The CSF was evaluated in 26 patients with high risk for CSF relapse: 14 with postlaminar optic nerve invasion, five of them with tumour at the resection margin, five with massive choroidal invasion, three with overt orbital extension and four patients with systemic metastasis. Serial CSF examinations were repeated at different time intervals according to stage and in the event of suspected relapse. GD2 synthase mRNA was evaluated by RT and nested PCR at each procedure. Results: MDD was present at diagnosis in six cases (23%) and it was significantly associated to massive optic nerve involvement or history of glaucoma (p < 0.05). Three of the children with positive MDD had a CSF relapse. Thirteen patients had negative MDD at diagnosis and one had a CSF relapse. In seven children no ARN could be obtained for PCR analysis and two subsequently relapsed. The probability of CSF relapse was 0.50 (95% confidence interval (CI) 0.13–0.88) for children with MDD and 0.08 (95% CI 0.02–0.46) for those with negative RT-PCR examination of the CSF at diagnosis (p = 0.03). Conclusions: MDD in the CSF detected by RT-PCR for GD2-synthase mRNA occurred in 31.7% of evaluable high-risk children with retinoblastoma with no initial central nervous system (CNS) involvement. It was significantly associated to optic nerve involvement and glaucoma and increased risk of CSF relapse.Fil: Laurent, Viviana Eunice. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan". Servicio de Hemato-Oncología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sampor, Claudia. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan". Servicio de Hemato-Oncología; ArgentinaFil: Solernou, Verónica. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan". Servicio de Patología; ArgentinaFil: Rossi, Jorge. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Gabri, Mariano Rolando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Eandi Eberle, Silvia. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan". Servicio de Hemato-Oncología; ArgentinaFil: García de Dávila, María T.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan". Servicio de Hemato-Oncología; ArgentinaFil: Alonso, Daniel Fernando. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Oncología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chantada, Guillermo Luis. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan". Servicio de Hemato-Oncología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Six novel variants in the PKLR gene associated with pyruvate kinase deficiency in Argentinian patients

Background: Pyruvate kinase deficiency (PKD) is a rare recessive congenital hemolytic anemia caused by mutations in the PKLR gene. The disease shows a marked variability in clinical expression. We studied the molecular features of nine unrelated Argentinian patients with congenital hemolytic anemia associated with erythrocyte pyruvate kinase deficiency. Design and Methods: Routine hematologic investigations were performed to rule out other causes of chronic hemolytic anemia. Sanger sequencing and in-sílico analysis were carried out to identify and characterize the genetics variants. Results: Six different novel missense variants were detected among the 18 studied alleles: c.661 G > C (Asp221His), c.956 G > T (Gly319Val), c.1595 G > C (Arg532Pro), c.347 G > A (Arg116Gln), c.1232 G > T (Gly411Val), c.1021G > A (Gly341Ser). Structural implications of amino-acid substitutions were correlated with the clinical phenotypes seen in the probands. Conclusions: This is the first comprehensive report on molecular characterization of pyruvate kinase deficiency in Argentina and the second from South America that would contribute to our knowledge on the distribution and frequency of PKLR variants in our population but also offer new insights into the interpretation of the effect of PKLR variants and phenotype.Fil: Milanesio, Berenice. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Pepe, Carolina Mariana. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Defelipe, Lucas Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Eandi Eberle, Silvia. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Avalos Gomez, Vanesa. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Chaves, Alejandro. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Albero, Agustina. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Aguirre, Fernando. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Fernandez, Diego. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Aizpurua, Luciana. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Paula Dieuzeide, María. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; ArgentinaFil: Turjanski, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Bianchi, Paola. Ospedale Maggiore Policlinico Milano; ItaliaFil: Fermo, Elisa. Ospedale Maggiore Policlinico Milano; ItaliaFil: Feliu Torres, Aurora. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentin

Is it time to describe new species without diagnoses? -A comment on Sharkey et al. (2021)

Ahrens, Dirk, Ahyong, Shane T., Ballerio, Alberto, Barclay, Maxwell V. L., Eberle, Jonas, Espeland, Marianne, Huber, Bernhard A., Mengual, Ximo, Pacheco, Thaynara L., Peters, Ralph S., Rulik, Björn, Vaz-De-Mello, Fernando, Wesener, Thomas, Krell, Frank-Thorsten (2021): Is it time to describe new species without diagnoses? -A comment on Sharkey et al. (2021). Zootaxa 5027 (2): 151-159, DOI: https://doi.org/10.11646/zootaxa.5027.2.

Long-term monitoring of high-elevation terrestrial and aquatic ecosystems in the Alps - a five-year synthesis

Whether and how alpine organismic communities respond to ongoing environmental changes is difficult to assess quantitatively, given their intrinsically slow responses, remote locations and limited data. Here we provide a synthesis of the first five years of a multidisciplinary, highly standardized, long-term monitoring programme of terrestrial and aquatic ecosystems in the Austrian Hohe Tauern National Park and companion sites in northern Italy and the central Swiss Alps. The programme aims at evidencing the ecological state and trends in largely late-successional, high-elevation ecosystems. We present the conceptual framework, the study design and first results. Replicated over five regions, different sites and a multitude of permanent plots, the abiotic (microclimate, physics and chemistry of soils and water bodies), biodiversity (plants, animals, microbes), and productivity data (alpine grassland, lakes, streams) provide a representative reference for future re-assessments. The wide spectrum of biological baseline data presented and their spatial and temporal variation also illustrate the degree of uncertainty associated with smaller-scale and short-term studies and the role of stochasticity in long-term biological monitoring