The Dynamics Of Root Growth And The Partitioning Of Photosynthates In Cool Desert Shrubs

This study addresses the nature of physiological and phenological evolutionary strategies of root growth dynamics and energy allocation followed by Atriplex confertifolia, Ceratoides lanata, and Artemisia tridentata growing in their natural cool desert environment. Root observation chambers with inclined Plexiglass windows were installed in monospecific desert communities of Atriplex confertifolia, Ceratoides lanata and Artemisia tridentata. Soil temperature and water potential measurements taken immediately adjacent to the observation windows indicated a minimal disturbance was caused by the presence of these chambers. For the three species initiation of root growth was observed before initiation of shoot activity, furthermore, active root growth extended over much longer periods during the year than shoot growth. Initial growth was observed for the three species in the upper soil layers in the spring. Later in the season most of the growth activity was measured at progressively greater depths in the soil. Measurable root growth was observed for Atriplexin August when the soil water potentials were in the range of -70 bars for 1972, and at water potentials of -60 bars for all three species during 1973. Detectable growth for these three species was recorded as late as January in 1974. Except for the main extension roots, individual apical meristems were seldom active for more than 2 weeks. Atriplex confertifolia and Ceratoides lanata plots were labeled during the growth season with 14Co2 in polyethylene enclosures to study both the partitioning of photosynthates to plant parts and their total allocation of carbon at the community level. A definite seasonal pattern of partitioning of recent photoassimilates corresponding to phenological events emerged. In the spring, photoassimilates were principally directed to shoot growth, especially expanding new leaves and vegetative buds. In terms of relative energy allocated to plant parts per unit dry weight basis, it appears that Ceratoides lanata expends less energy for reproductive organs. For both species, carbon used for new stems and previous years shoot growth appears to constitute a significant sink for energy use and storage. Relative translocation of carbon to roots was minimal during the spring for both species. It increased with the progression of the season reaching a maximum in July for Atriplex and at the end of the season for Ceratoides. Energy allocation at the community level for these species showed that approximately 60 and 40 percent of the recently photoassimilated 14C for the Atriplex-dominated community in July and September, respectively, appeared localized in the new shoot growth, the remaining was distributed in nearly equal amounts between previous year\u27s shoot growth and the root system. The scheme of energy allocation in Ceratoides showed similar patterns of carbon utilization in July and September; approximately 80 percent of the fixed carbon was al located in approximately equal amounts to roots and new shoot growth with the remainder to the previous year\u27s shoot growth. In the Ceratoides-dominated community 65 percent and 36 percent of 14C photoassimilated in April and July, respectively, and still remaining in the plant by September, was localized in the underground structures. Similarly, in the Atriplex community, 35 percent and 29 percent of the 14C incorporated in April and July appeared in the root system. From the total 14C photoassimilated in July for both communities, approximately 60 percent and 50 percent was retained in the plants by September in the Atriplex- and Ceratoides-dominated communities, respectively

A review on invasive plants in rangelands of Argentina

In Argentina, information on invasive plants is restricted to croplands, while no information is available on rangelands. Our objectives were to 1) identify and describe various characteristics of the most important non-native species that have become invasive and widespread in major rangeland territories of Argentina; 2) summarize the biological information about some naturalized, non-native species with potential to transform natural plant communities, and 3) discuss strategies for plant invasion management and biodiversity conservation in local rangeland ecosystems. The invasive species most represented at local, regional or state scale studies were Acroptilon repens, Centaurea solstitialis, Eleagnus angustifolia, Medicago minima, Chondrilla juncea, Dipsacus sativus and Sorghum halepense. Successful invasion and naturalization rates have been the result of several combined ecological traits: 1) capacity to produce allelophatic compounds, 2) deep rooting, 3) high module density, 4) rapid vegetative spread aboveground, 5) various traits that make species highly competitive, 6) tolerance to shading and water stress, 7) ability to take advantage of disturbances, 8) high seed production, germination and dispersal, and 9) high viability of residual seed banks. The determination of the abundance of invasive species at country scale, and their ecological and economical damage, are objectives of future research. This information will be a critical tool to make decisions on the need to control invasive species. Ecological studies providing understanding of the strategies which make an invader species a successful competitor are critical, and should be the first step to establish policies for control of invasive species and use of rangelands.Fil: Busso, Carlos Alberto. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. State University Of Utah; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); ArgentinaFil: Bentivegna, Diego Javier. Universidad Nacional del Sur; Argentina. University Of Missouri; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernandez, Osvaldo Alberto. Universidad Nacional del Sur; Argentina. State University Of Utah; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

A Note on the Log-Alpha-Skew-Normal Model with Geochemical Applications.

In this paper we introduce an extension of the log-normal distribution, based on the alpha-skew-normal distribution introduced by Elal-Olivero [10]. Basic properties, moments, moment estimators, maximum likelihood estimators and a simulation study are discussed. We apply the approach developed in this paper to data sets related to neodymium and nickel concentrations in soil samples. Model fit indicates good performance of the proposed model when compared with less flexible models

Variability and correlations of traits related to seedling vigor in two natural populations of Pappophorum vaginatum Buckley

Se evaluó variabilidad y correlación fenotípica en caracteres vinculados con el vigor de plántula en dos poblaciones de P. vaginatum. Se pesaron y embraron individualmente 100 cariopses por población ("A"; "B"). A los 43 días se determinó: longitud aérea (LA), radical (LR), total (LT) y de raíz adventicia más larga (Ladv); número de raíces adventicias (n°adv), hojas (n°hoj), hojas senescentes (n°sen) y macollos (n°mac) y peso seco aéreo (PSA) y radical (PSR). Se calculó peso seco total (PST), PSA/PSR y LA/LR. La variabilidad se analizó mediante estadística descriptiva y prueba de t. Se analizó correlación entre caracteres y regresión múltiple de los caracteres sobre PST. Las poblaciones no difirieron en PST (p≥0,05). "A" presentó mayor (p≤0,01) PSA/ PSR, LA/ LR y LA. "B" tuvo mayor (p≤0,05) LR, Ladv y PSR. Ambas poblaciones presentaron correlación significativa y positiva del PST con LA, LT, Ladv, n°adv, n°hoj y n°mac. La regresión fue significativa (p≤0,01). La variabilidad encontrada dentro y entre las poblaciones implica expectativas de respuesta a la selección. PST varió sólo dentro de poblaciones sugiriendo la conveniencia de selección intrapoblacional. La asociación encontrada entre los caracteres LA y n°mac y el vigor de plántula, debería ser considerada en la mejora genética de la implantación.It was evaluated variability and phenotypic correlation in traits related to seedling vigor in two natural populations of P.vaginatum. They were weighted and sowed individually 100 caryopses of each population ("A"; "B"). After 43 days it was determined: lenghts aerial (LA), root (LR), total (LT) and longest adventitious root (Ladv), number of adventitious root (n°adv), of leaves (n°hoj), of senescent leaves (n°sen) and of tillers (n°mac) and dry weight aerial (PSA) and root (PSR). It was calculated total dry weight (PST), PSA/PSR and LA/LR. Variability was analyzed by means of descriptive statistic and t test. It was analyzed correlation among traits and multiple regression of traits on PST. Populations did not differ in PST (p≥0.05). "A" had greater (p≤0.01) PSA/PSR, LA/LR and LA. "B" had higher (p≤0.05) LR, Ladv and PSR. Both populations showed significant and positive correlation of PST with LA, LT, Ladv, n°adv, n°hoj y n°mac. The regression was significant (p≤0.01). The variability found within and between populations implies expectations of response to selection. PST varied only within populations suggesting the convenience of intrapopulation selection. The association found between the traits LA and n°mac and the seedling vigor, should be considered for the genetic improvement of the implantation.Fil: Entio, Lisandro Jose. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Mujica, Maria de la Merced. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Busso, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Torres, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Montenegro, Oscar Alberto. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios; ArgentinaFil: Ithurrart, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Giorgetti, Hugo Dosindo. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios; ArgentinaFil: Rodriguez, Gustavo Dionisio. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios; ArgentinaFil: Bentivegna, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Brevedan, Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Fernandez, Osvaldo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Baioni, Sandra Sonia. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Fioretti, Maria Nelida. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Tucat, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentin

Efecto fitotóxico de Baccharis ulicina sobre la germinación y crecimiento inicial de Avena sativa, Lolium perenne y Raphanus sativus

Baccharis ulicina es una maleza ampliamente distribuida en los pastizales de la zona semiárida argentina. A fin de evaluar sus posibles efectos alelopáticos, se utilizaron extractos acuosos de B. ulicina (hoja, tallo, raíz y planta entera) a dos concentraciones (50 y 150 g tejido/L agua) sobre la germinación y crecimiento inicial de Avena sativa , Lolium perenne y Raphanus sativus . Para cada especie se regaron 100 semillas y se mantuvieron a 25°C y oscuridad. Se registraron la germinación de las semillas y la longitud de coleóptilo / hipocótile (C/H) y radícula (R). Con los datos obtenidos se determinó: porcentaje de germinación, coeficiente de velocidad de germinación, vigor de la germinación, inhibición de la germinación, inhibición de la longitud de la radícula y del coleóptilo / hipocótile e índice de vigor de radícula y de coleóptilo / hipocótile. Los extractos con alta concentración redujeron la germinación en promedio 65% mientras que inhibieron la longitud en 58,8% (C/H) y 93,3% (R) para A. sativa , 71,5% (C/H) y 87,9% (R) para L. perenne , y 93,8% (C/H) y 94,5% (R) para R. sativus . Se registró un evidente efecto fitotóxico de B. ulicina sobre las especies evaluadas, indicando que la alelopatía sería una estrategia ecológica de la especie.Baccharis ulicina is a weed widely distributed in pastures of the semiarid zone of Argentina. With the aim of evaluate potential allelopathic effects, aqueous extracts of B. ulicina (leaf, stem, root and whole plant) at two concentrations (50 and 150 g tissue/L water) were used on germination and initial growth of Avena sativa, Lolium perenne and Raphanus sativus. For each species, 100 seeds were watered and maintained at 25°C at darkness. Seed germination and length of coleoptile / hypocotyl (C/H) and radicle (R) was measured. Then, germination percentage, germination rate coefficient, germination vigor, inhibition of germination, inhibition of radicle length and coleoptile / hypocotyl length, radicle vigor index and the coleoptile / hypocotyl vigor index were calculated. High concentration extracts reduced germination an average of 65%, in addition, they produced a length inhibition of 58.8% (C/H) and 93.3% (R) to A. sativa, 71.5% (C/H) and 87.9% (R) to L. perenne, and 93.8% (C/H) and 94.5% (R) to R. sativus. There was an evident phytotoxic effect of B. ulicina on evaluated species, indicating that allelopathy would be an ecological strategy of the species.Fil: Tucat, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Bahia Blanca. Centro Rec.nat.renovables de Zona Semiarida(i); Argentina;Fil: Bentivegna, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Bahia Blanca. Centro Rec.nat.renovables de Zona Semiarida(i); Argentina;Fil: Fernandez, Osvaldo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Bahia Blanca. Centro Rec.nat.renovables de Zona Semiarida(i); Argentina;Fil: Busso, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Bahia Blanca. Centro Rec.nat.renovables de Zona Semiarida(i); Argentina;Fil: Brevedan, Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Bahia Blanca. Centro Rec.nat.renovables de Zona Semiarida(i); Argentina;Fil: Mujica, Maria de la Merced. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Ambiente y Recursos Naturales. Cat.introduccion de Mejoramiento Genético; Argentina;Fil: Torres, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Bahia Blanca. Centro Rec.nat.renovables de Zona Semiarida(i); Argentina;Fil: Daddario, Juan Facundo Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Bahia Blanca. Centro Rec.nat.renovables de Zona Semiarida(i); Argentina;Fil: Ithurrart, Leticia Soledad. Universidad Nacional del Sur; Argentina;Fil: Giorgetti, Hugo Dosindo. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios. Chacra Experimental de Patagones; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;Fil: Rodriguez, Gustavo Dionisio. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios. Chacra Experimental de Patagones; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;Fil: Montenegro, Oscar Alberto. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios. Chacra Experimental de Patagones; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;Fil: Baioni, Sandra Sonia. Universidad Nacional del Sur; Argentina;Fil: Entio, Lisandro Jose. Universidad Nacional de la Plata. Facultad de Cs.agrarias y Forestales. Departamento de Ambiente y Recursos Naturales. Cat.introduccion de Mejoramiento Genético; Argentina;Fil: Fioretti, Maria Nelida. Universidad Nacional del Sur; Argentina

Plant growth and survival of five introduced and two native/naturalized perennial grass genotypes exposed to two defoliation managements in arid Argentina

The field performance of the native Pappophorum vaginatum, the naturalized Eragrostis curvula and various cultivars of the introduced Achnatherum hymenoides and Leymus cinereus was evaluated as potential forage resources in rangelands of arid Argentina during the warm seasons of 2007/2008 and 2008/2009. Plants of these grass species, obtained from seeds, were transplanted to the field in 2006, when they were 1 year old. During the study years, there were two defoliation managements: plants of all study genotypes either remained undefoliated (controls) or were defoliated twice a year during spring at 5 cm stubble height. Despite tiller number being lower (P 0·05) between defoliation managements by mid-spring, there were no differences (P > 0·05) in dry weight production between defoliated and undefoliated plants in all genotypes at the end of the second growing season. Plants of one or more of the introduced genotypes showed a similar (P > 0·05) or greater (P 0·05) or greater (P 70%) or naturalized (>39%) genotypes at the end of the first or second growing seasons.Facultad de Ciencias Agrarias y Forestale

Root proliferation in native perennial grasses of arid Patagonia, Argentina

Pappophorum vaginatum is the most abundant C4 perennial grass desirable to livestock in rangelands of northeastern Patagonia, Argentina. We hypothesized that (1) defoliation reduce net primary productivity, and root length density and weight in the native species, and (2) root net primary productivity, and root length density and weight, are greater in P. vaginatum than in the other, less desirable, native species (i.e., Aristida spegazzinii, A. subulata and Sporobolus cryptandrus). Plants of all species were either exposed or not to a severe defoliation twice a year during two growing seasons. Root proliferation was measured using the cylinder method. Cylindrical, iron structures, wrapped up using nylon mesh, were buried diagonally from the periphery to the center on individual plants. These structures, initially filled with soil without any organic residue, were dug up from the soil on 25 April 2008, after two successive defoliations in mid-spring 2007. During the second growing season (2008–2009), cylinders were destructively harvested on 4 April 2009, after one or two defoliations in mid-and/or late-spring, respectively. Roots grown into the cylinders were obtained after washing the soil manually. Defoliation during two successive years did reduce the study variables only after plants of all species were defoliated twice, which supported the first hypothesis. The greater root net primary productivity, root length density and weight in P. vaginatum than in the other native species, in support of the second hypothesis, could help to explain its greater abundance in rangelands of Argentina.Facultad de Ciencias Agrarias y Forestale

Water stress and temperature effects on germination and early seedling growth of Digitaria eriantha

This study focused on the two major processes critical for plant establishment: Seed germination and seedling survival. We determined the effects of (1) water stress and temperature on the germination, and (2) water stress on early seedling growth of Digitaria eriantha cv ‘Irene’. Seeds harvested in 2007 were used for temperature studies, and those coming from 2006 and 2007 for water stress studies. In 2009, viability decreased by 65.4% from 2006 to 2007. During the first twenty-four hours, germination was more than 50% at constant (30 or 35°C) than alternating (10/30 or 10/35°C) temperatures, although total germination was about 80% for all temperature treatments. Polyethylene glycol 8000 was used to impose water stress conditions. Germination percentages and coefficients of velocity decreased with decreasing water potentials. Early seedling growth was smaller at lower water potentials. D. eriantha cv ‘Irene’ appeared to germinate within a wide range of temperatures, but it varied greatly in germination response to water potentials. Results suggest that this species could be planted in late spring-early summer, when seedbed temperatures are increasing and soil moisture might still be adequate.Fil: Brevedan, Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Busso, Carlos Alberto. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); ArgentinaFil: Fioretti, M. N.. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Toribio, M. B.. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Baioni, S. S.. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Torres, Yanina Alejandra. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); ArgentinaFil: Fernandez, Osvaldo Alberto. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); ArgentinaFil: Giorgetti, H. D.. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios. Chacra Experimental de Patagones; ArgentinaFil: Bentivegna, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); ArgentinaFil: Entío, J.. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Ithurrart, Leticia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Montenegro, O.. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios. Chacra Experimental de Patagones; ArgentinaFil: Mujica, M. de las M.. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Rodríguez, G.. Provincia de Buenos Aires. Ministerio de Asuntos Agrarios. Chacra Experimental de Patagones; ArgentinaFil: Tucat, Guillermo. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentin

Canagliflozin and renal outcomes in type 2 diabetes and nephropathy

BACKGROUND Type 2 diabetes mellitus is the leading cause of kidney failure worldwide, but few effective long-term treatments are available. In cardiovascular trials of inhibitors of sodium–glucose cotransporter 2 (SGLT2), exploratory results have suggested that such drugs may improve renal outcomes in patients with type 2 diabetes. METHODS In this double-blind, randomized trial, we assigned patients with type 2 diabetes and albuminuric chronic kidney disease to receive canagliflozin, an oral SGLT2 inhibitor, at a dose of 100 mg daily or placebo. All the patients had an estimated glomerular filtration rate (GFR) of 30 to <90 ml per minute per 1.73 m2 of body-surface area and albuminuria (ratio of albumin [mg] to creatinine [g], >300 to 5000) and were treated with renin–angiotensin system blockade. The primary outcome was a composite of end-stage kidney disease (dialysis, transplantation, or a sustained estimated GFR of <15 ml per minute per 1.73 m2), a doubling of the serum creatinine level, or death from renal or cardiovascular causes. Prespecified secondary outcomes were tested hierarchically. RESULTS The trial was stopped early after a planned interim analysis on the recommendation of the data and safety monitoring committee. At that time, 4401 patients had undergone randomization, with a median follow-up of 2.62 years. The relative risk of the primary outcome was 30% lower in the canagliflozin group than in the placebo group, with event rates of 43.2 and 61.2 per 1000 patient-years, respectively (hazard ratio, 0.70; 95% confidence interval [CI], 0.59 to 0.82; P=0.00001). The relative risk of the renal-specific composite of end-stage kidney disease, a doubling of the creatinine level, or death from renal causes was lower by 34% (hazard ratio, 0.66; 95% CI, 0.53 to 0.81; P<0.001), and the relative risk of end-stage kidney disease was lower by 32% (hazard ratio, 0.68; 95% CI, 0.54 to 0.86; P=0.002). The canagliflozin group also had a lower risk of cardiovascular death, myocardial infarction, or stroke (hazard ratio, 0.80; 95% CI, 0.67 to 0.95; P=0.01) and hospitalization for heart failure (hazard ratio, 0.61; 95% CI, 0.47 to 0.80; P<0.001). There were no significant differences in rates of amputation or fracture. CONCLUSIONS In patients with type 2 diabetes and kidney disease, the risk of kidney failure and cardiovascular events was lower in the canagliflozin group than in the placebo group at a median follow-up of 2.62 years