Escrito en los objetos, latiendo en los espacios. Historia de vida y memoria de Paulino Roa, vecino múltiple de Pipaón (Álava, España)

The article presents an experimental life story and offers theorethical-methodological reflections about its construction. When Paulino Roa talk about his life, assimilates the character of exceptionality and representativity that social researchers, journalists and neighbors of Pipaón town has granted to him, and following this process, ends up being a character of this story himself. However, his tale lacks of a linear narrative which is a characteristic of the conventionallife stories. Five are the existential spaces where Paulino’s life occurs (vegetable garden, kitchen, workshop-entrance, church and museum), the objects found there organize, thanksto the memory, a story that condense two superposed temporalities: the immediate present and the daily life -Paulino’s life, his wife, his son, his granddaughter, the town today-; and an actualization of the historic memory of the community -the war, the rural jobs, the splendor of Pipaón and its traditions-. The problems presented during the elaboration of the story are the starting point to explore textual possibilities, innovative and creative, to develop the biographic method in the Anthropology.Este artículo presenta una historia de vida experimental y ofrece reflexiones teórico-metodológicas sobre su construcción. Al narrar su vida, Paulino Roa asimila el carácter de excepcionalidad y, al mismo tiempo, de representatividad que investigadores sociales, periodistas y vecinos del pueblo de Pipaón le han otorgado, siguiendo un proceso por el que termina convertido en su propio personaje. Pero su narración omite la estructura lineal y cronológica de las historias de vidaconvencionales. Son los cinco espacios existenciales donde transcurre su vida (huerto, cocina, taller-portal, iglesia y museo) y los objetos allí contenidos los que organizan, gracias a la memoria, un relato que condensa dos temporalidades superpuestas: el presente inmediato y cotidiano –las vida de Paulino, su esposa, su hijo, su nieta, el pueblo de ahora–; y una reactualización de la memoria histórica de la comunidad –la guerra, los oficios rurales, el esplendor de Pipaón y sus tradiciones–. Los problemas surgidos en la elaboración de la historia son el punto de partida para explorar algunas de las posibilidades textuales, innovadoras y creativas, del método biográfico en antropologí

The highly virulent variola and monkeypox viruses express secreted inhibitors of type I interferon

Variola virus (VARV) caused smallpox, one of the most devastating human diseases and the first to be eradicated, but its deliberate release represents a dangerous threat. Virulent orthopoxviruses infecting humans, such as monkeypox virus (MPXV), could fill the niche left by smallpox eradication and the cessation of vaccination. However, immunomodulatory activities and virulence determinants of VARV and MPXV remain largely unexplored. We report the molecular characterization of the VARV- and MPXV-secreted type I interferon-binding proteins, which interact with the cell surface after secretion and prevent type I interferon responses. The proteins expressed in the baculovirus system have been purified, and their interferon-binding properties characterized by surface plasmon resonance. The ability of these proteins to inhibit a broad range of interferons was investigated to identify potential adaptation to the human immune system. Furthermore, we demonstrate by Western blot and activity assays the expression of the type I interferon inhibitor during VARV and MPXV infections. These findings are relevant for the design of new vaccines and therapeutics to smallpox and emergent virulent orthopoxviruses because the type I interferon- binding protein is a major virulence factor in animal models, vaccination with this protein induces protective immunity, and its neutralization prevents disease progression

Identification of RSVP14 and RSVP20 Components by Two-dimensional Electrophoresis and Western-blotting

Contents We have already shown that RSVP14 and RSVP20, two ram seminal plasma (SP) proteins postulated to be involved in sperm capacitation and gamete interaction can protect spermatozoa against cold-shock. In this study, we use twodimensional polyacrylamide gel electrophoresis (2D-PAGE) for the analysis of SP proteins of Rasa Aragonesa rams, using enhanced protein solubilization in the presence of tributyl phosphine (TBP) and a polyacrylamide linear gradient gel with a narrow pH range (4-7). The image analysis of the 2D map detected 195 protein spots, with isoelectric points (pIs) ranging from 4.5 to 6.6, and molecular weight (M r ) from 11.7 to 90.4. Staining of 2D gels with Pro-Q Emerald 300 Glycoprotein Stain revealed that most significant proteins in ram SP are glycosylated. The removing of protein N-linked oligosaccharides improved the gel resolution. 2D-PAGE analysis of the whole fraction 6 (F6) separated from ram SP by exclusion chromatography showed six main protein spots, four (a, b, c, d) in the 14 kDa and two (e, f) in the 20 kDa region. Western-blot analyses indicated that the anti-P14 antibody recognized four spots on the SP map, 4, 5, 6 and 7, that matched with spots a, b, c, d of F6 map. The anti-P20 antibody recognized spots 13 and 14 of SP map that corresponded to spots e, f of F6 map. The deduced sequences by de novo sequencing evidenced that protein spots 7 and 13 have significant similarities to BSP family, while protein spots 4 and 14 did not appear to be homologous with any reported protein in the current mammalian Proteinbank databases

Interplay between detrital and diagenetic processes since the last glacial maximum on the northwest Iberian continental shelf

Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Quaternary Research 73 (2010): 507-520, doi:10.1016/j.yqres.2010.02.003.Integrated analyses of magnetic, geochemical and textural data on six cores from the northwestern Iberian continental shelf allowed the reconstruction of the paleoenvironmental evolution of this area since the last glacial maximum (LGM). Four sedimentary units were identified, representing a succession from fluvial and subaerial settings to high and finally low-energy marine deposits subsequent to the post-LGM sea evel rise. The uppermost unit was deposited during the Holocene and its magnetic properties were controlled by the interplay between detrital input and early diagenetic reductive dissolution of magnetic minerals. Identification of a primary steady-state early diagenetic signal allowed the recognition of periods of increased detrital input bounded by intervals of lower detrital input and intensified reductive diagenesis related related to upwelling intensifications in the area. These paleoenvironmental alternations are consistent with the climatic evolution of the late Holocene. During the Roman Warm Period (RWP) and Medieval Warm Period (MWP), the combined effect of greater humidity and intense agricultural and mining activities led to a greater erosion and transport of detrital sediments to the shelf. In contrast, enhanced diagenetic reduction intervals, caused by upwelling intensification, were roughly coincident with the colder Dark Ages (DA) and the Little Ice Age (LIA).Contribution to projects CTM2007-61227/MAR and REN2003-02822 funded by the Spanish MICINN. Partial contribution to projects PGIDIT06TAM31201PR and IN825B funded by Xunta de Galicia and NACSA 236962 and ANCORIM funded by the EU. The EU Paleostudies program funded the stay of Kais J. Mohamed in Bremen. K. J. Mohamed wishes to acknowledge joint funding from the Fulbright Program and the Spanish Ministry of Education and Science and the Marie Curie International Outgoing Fellowships within the 7th European Community Framework Programme

Immediate changes in radiographically determined lateral flexion range of motion following a single cervical HVLA manipulation in patients presenting with mechanical neck pain: A case series

Abstract Background: It is generally assumed that inter-vertebral joint dysfunction results in a temporary reduction of mobility of a spinal segment and it has been purported that spinal manipulation can directly affect the biomechanical behaviour of the spine. Functional X-rays are used to assess dynamic alterations of spinal function. Objective: The aim of this case series was to describe the immediate changes of inter-vertebral motion at an identified dysfunctional cervical segment, as measured by functional X-rays in lateral flexion, following a supine cervical rotation manipulation in patients presenting with mechanical neck pain. Methods: Fifteen patients who presented with mechanical neck pain and who exhibited inter-vertebral joint dysfunction at C3eC4 or C4eC5 levels were recruited to participate in this case series. The radiological distance between the transverse process of the identified hypomobile vertebra and the transverse process of the subjacent vertebra, was measured pre-and 5 min post-manipulation during contralateral side flexion. Results: Analysis of the pre-post-intervention radiographs showed a significant increase (P ¼ 0.01) of the distance between the transverse process on the dysfunctional side following cervical manipulation. The mean pre-manipulative inter-vertebral radiological measurement was 18.9 mm (SD 2.1), and 20.6 mm (SD 2.1) at the post-manipulative assessment. Conclusions: These preliminary results demonstrated a trend toward an increase in inter-vertebral motion at the hypomobile segment, measured by functional radiography

Safety and efficacy of Lactobacillus plantarum NCIMB 42150 as a silage additive for all animal species

The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety and efficacy of a specific strain of Lactobacillus plantarum when used as a technological additive intended to improve ensiling at a proposed application rate of 1 9 108 colony forming unit (CFU)/kg fresh material. L. plantarum is considered by EFSA to be suitable for the qualified presumption of safety approach to safety assessment and not to require specific demonstration of safety other than demonstrating the absence of resistance to antibiotics of human and veterinary significance. As the identity of the strain was clearly established and as no antibiotic resistance was detected, the use of the strain in the production of silage is presumed safe for livestock species, consumers of products from animals fed treated silage and the environment. In the absence of data, no conclusion can be drawn on the skin and eye irritancy or skin sensitisation of the additive. The additive should be considered as a potential respiratory sensitiser. Five studies with laboratory-scale silos were made using forage of differing water-soluble carbohydrate content. Replicate silos containing forages treated at the proposed application rate were compared to identical silos containing the same but untreated forage. In addition, in four studies, formic acid was included as positive control. The mini-silos were then stored for 90\u2013103 days at 20\u201324\ub0C. After opening, the contents of the silos were analysed. Results showed that this strain of L. plantarum has the potential to improve the production of silage from easy, moderately difficult and difficult to ensile forage species by increasing the production of lactic acid, reducing the pH and increasing the preservation of dry matter when used at an application rate of 1 9 108 CFU/kg

Cardiopoietic cell therapy for advanced ischemic heart failure: results at 39 weeks of the prospective, randomized, double blind, sham-controlled CHART-1 clinical trial

Cardiopoietic cells, produced through cardiogenic conditioning of patients' mesenchymal stem cells, have shown preliminary efficacy. The Congestive Heart Failure Cardiopoietic Regenerative Therapy (CHART-1) trial aimed to validate cardiopoiesis-based biotherapy in a larger heart failure cohort

Investigations of transmission nonlinearities and mode-locked laser encoders for ultrafast optical CDMA networks

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