868 research outputs found
La aparotologĂa de tracciĂłn extraoral
Si bien es cierto que el concepto moderno de la aparatologĂa ortodĂłncica, tiene como ideal el uso de aparatos sencillos, que no sean visibles y que no requieran de la cooperaciĂłn del paciente para el logro de los resultados esperados, es la fuerza de la aparatologĂa extraoral en uniĂłn con todas las modalidades de mecanoterapia contemporáneas, quiĂ©n nos permite lograr una correcciĂłn de la más alta calidad y con mayor estabilidad de las maloclusiones de Clase II.Si bien es cierto que el concepto moderno de la aparatologĂa ortodĂłncica, tiene como ideal el uso de aparatos sencillos, que no sean visibles y que no requieran de la cooperaciĂłn del paciente para el logro de los resultados esperados, es la fuerza de la aparatologĂa extraoral en uniĂłn con todas las modalidades de mecanoterapia contemporáneas, quiĂ©n nos permite lograr una correcciĂłn de la más alta calidad y con mayor estabilidad de las maloclusiones de Clase II
Fish β-parvalbumin acquires allergenic properties by amyloid assembly
PRINCIPLES: Amyloids are highly cross-β-sheet-rich aggregated states that confer protease resistance, membrane activity and multivalence properties to proteins, all essential features for the undesired preservation of food proteins transiting the gastrointestinal tract and causing type I allergy. METHODS: Amyloid propensity of β-parvalbumin, the major fish allergen, was theoretically analysed and assayed under gastrointestinal-relevant conditions using the binding of thioflavin T, the formation of sodium dodecyl sulphate- (SDS-) resistant aggregates, circular dichroism spectroscopy and atomic force microscopy fibril imaging. Impact of amyloid aggregates on allergenicity was assessed with dot blot. RESULTS: Sequences of β-parvalbumin from species with commercial value contain several adhesive hexapeptides capable of driving amyloid formation. Using Atlantic cod β-parvalbumin (rGad m 1) displaying high IgE cross-reactivity, we found that formation of amyloid fibres under simulated gastrointestinal conditions accounts for the resistance to acid and neutral proteases, for the presence of membrane active species under gastrointestinal relevant conditions and for the IgE-recognition in the sera of allergic patients. Incorporation of the anti-amyloid compound epigallocatechin gallate prevents rGad m 1 fibrillation, facilitates its protease digestion and impairs its recognition by IgE. CONCLUSIONS: the formation of amyloid by rGad m 1 explains its degradation resistance, its facilitated passage across the intestinal epithelial barrier and its epitope architecture as allergen.Peer Reviewe
Influence of methane and carbon monoxide in the volumetric behaviour of the anthropogenic CO2: Experimental data and modelling in the critical region
Density measurements of the systems CO 2 + CH 4 at T = 304.21 K and CO 2 + CO at T = 304.21 and 308.15 K were carried out at pressures and compositions of interest in the process of transport and storage of
CO 2 (CCS technology): P = 0.1 to 20 MPa, and xCO 2 ≥0.97. From the experimental P––T –xCO 2 data, we have studied the dependence of the isothermal compressibility, the excess molar volume, and the par-
tial molar volume of the solute with composition, pressure and temperature within the critical region. We have modelled the volumetric behaviour of these systems with Peng–Robinson, PC-SAFT and GERG
Equations of State, thus validating these equations under the conditions considered. Furthermore, we have confirmed the weakly attractive behaviour of these systems by performing a study of the interac-
tions between solvent molecules, CO 2 , and those of the solute, CO or CH 4 , using the Krichevskii Function concept and the Kirkwood–Buff Theory. Finally, we have quantified the influence of small amounts of
CH 4 and CO in the transport of anthropogenic CO 2 through several normalized parameters, related to the design and operation of the fluid transport process
O proceso de alfabetizaciĂłn en Galicia (1860-1991)
[Resumo] O obxectivo deste traballo é determinar o nivel cultural básico da
poboaciĂłn galega durante o perĂodo censal moderno. OfrĂ©cense, en primeiro lugar,
as taxas de alfabetización brutas entre 1860 e 1960, analizándose tamén a
extensiĂłn da semialfabetizaciĂłn e o analfabetismo. De seguido calcĂşlanse as taxas
de alfabetización netas desde 1887 a 1991, referidas á poboación de dez e máis
anos. A partir dos seus valores proponse un ha periodizaciĂłn do proceso de
alfabetizaciĂłn en Galicia.[Abstract] The purpose of this paper is to determine the basic cultural level of
the Galician population during the modern census periodo The gross data on
literacy rates between 1860 and 1960 are presented first with an analysis of the
extent of semi-literacy and illiteracy. The net literacy rates are then calculated from
1887-1991, based on members of the population 10 years of age or overo From the
resulting values, the different periods that the literacy process in Galicia goes
through are discussed
EAACI Task force Clinical epidemiology of anaphylaxis: experts’ perspective on the use of adrenaline autoinjectors in Europe
Background Worldwide, guidelines recommend the use of adrenaline autoinjectors (AAIs) for self-medication in patients who experience severe allergic reaction. The European Medical Agency recommends the prescription of two AAIs, which should be carried by patients at all times. The European Academy of Allergy and Clinical Immunology guidelines propose to prescribe a second AAI under some defined conditions. In the present study, we aimed to examine the adherence to these guidelines and prescription behavior of allergy experts regarding the number of AAIs prescribed for a given patient. Methods A standardized questionnaire was applied to the participants of the 5th International Conference of the Network of Online Registration for Anaphylaxis (NORA e. V.). Twenty-six experts (medical doctors with at least 2 years of experience in the field of anaphylaxis) answered the questions regarding the number of autoinjectors prescribed and the reasons influencing their decisions. Results Sixty-eight percent of the experts usually prescribed one AAI, while 32% prescribed two. The pediatricians and physicians with less experience tended to prescribe two autoinjectors more frequently. The experts were more likely to prescribe two adrenaline autoinjectors if the patient was a child, had a previous severe reaction, had mastocytosis, asthma, cardiovascular disease, or high body weight, or lived far from the emergency department. Conclusion Our data confirm the lack of consensus regarding the number of AAIs to prescribe. Despite the European Medical Agency recommendation, the majority of allergy experts prescribed one autoinjector per patient. However, under distinct circumstances (e.g. mastocytosis, asthma, excess body weight, a history of severe anaphylaxis, or restricted access to immediate emergency), experts tended to prescribe more AAIs, which is in accordance with the European Academy of Allergy and Clinical Immunology guidelines
High-pressure speed of sound in pure CO2 and in CO2 with SO2 as an impurity using methanol as a doping agent
Reliable speed of sound, c, values in CO2- rich mixtures and pure CO2 are required for carbon capture and storage (CCS) technology but are difficult to determine, particularly at relatively high frequencies. We tested the suitability of methanol as doping agent to obtain accurate c values in CCS systems at 5 MHz. We measured c in seven CO2-rich, CO2 + methanol mixtures between 263.15 and 323.15 K and up to 196.30 MPa, and we extrapolated the values to obtain c in pure CO2. Additionally, we measured c from 263.15 to 373.19 K and up to 190.10 MPa in two CO2-rich, CO2 + SO2 mixtures with the same SO2 composition, which is of interest for CCS, with one mixture doped with methanol. We compared our results for pure CO2 with the literature and the Span and Wagner equation of state (EoS). We validated the PC-SAFT EoS and the modeling with the REFPROP 9 software for the mixtures by comparing the predicted values with our experimental data under the studied conditions. We conclude that methanol is a suitable doping agent to measure c in pure CO2 and CO2-rich mixtures. For the CO2 + SO2 mixtures, the effect of methanol on the experimental values is small and negligible for modeling
Improved regeneration of eggplant doubled haploids from microspore-derived calli through organogenesis
[EN] Doubled haploid (DH) technology allows for
the production of pure lines, useful for plant breeding,
through a one-generation procedure that reduces considerably
the time and resources needed to produce them.
Despite the advantages of microspore culture to obtain
DHs, this technique is still insufficiently developed in
eggplant, where DHs are produced from microsporederived
calli through organogenesis. At present, very little
is known on the best in vitro conditions to promote this
process. This is why in this work we addressed the optimization
of the process of regeneration of eggplant DH
plants from microspore-derived calli. We evaluated the
effect of different media compositions in the induction of
organogenesis, in the promotion of shoot growth and
elongation, and in root growth. According to our results,
we propose the repeated subculture of the calli in MS
medium with 0.2 mg/l IAA and 4 mg/l zeatin to produce
shoots, and then the repeated subculture of the excised
shoots in basal MS medium to promote their conversion
into entire plantlets. This procedure yielded 7.6 plants per
100 cultured calli, which represents a *49 increase with
respect to previous reports. We also evaluated by flow
cytometry and SSR molecular markers the effect of these
in vitro culture conditions in the rate of DH plant production,
finding that*70 % of the regenerated plants were
true DHs. These results substantially improve the efficiencies
of DH recovery published to date in eggplant, and
may be useful to those working in the field of eggplant
doubled haploidy and breeding.We acknowledge Dr. Rosa Peiro for her statistical advice, and the staff of the COMAV greenhouses for their valuable help. This work was supported by the AGL2014-55177-R grant from Spanish MINECO to JMSS.Rivas Sendra, A.; Corral MartĂnez, P.; Camacho Fernández, C.; SeguĂ-Simarro, JM. (2015). Improved regeneration of eggplant doubled haploids from microspore-derived calli through organogenesis. Plant Cell, Tissue and Organ Culture. 122(3):759-765. https://doi.org/10.1007/s11240-015-0791-6S7597651223Asif M, Eudes F, Randhawa H, Amundsen E, Spaner D (2014) Phytosulfokine alpha enhances microspore embryogenesis in both triticale and wheat. Plant Cell Tissue Organ Cult 116:125–130Borgato L, Conicella C, Pisani F, Furini A (2007) Production and characterization of arboreous and fertile Solanum melongena plus Solanum marginatum somatic hybrid plants. Planta 226:961–969Castillo AM, Nielsen NH, Jensen A, VallĂ©s MP (2014) Effects of n-butanol on barley microspore embryogenesis. Plant Cell Tissue Organ Cult 117:411–418Corral-MartĂnez P, SeguĂ-Simarro JM (2012) Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.). Euphytica 187:47–61Corral-MartĂnez P, SeguĂ-Simarro JM (2014) Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins. Euphytica 195:369–382Dhooghe E, Van Laere K, Eeckhaut T, Leus L, Van Huylenbroeck J (2011) Mitotic chromosome doubling of plant tissues in vitro. Plant Cell Tissue Organ Cult 104:359–373Dumas de Vaulx R, Chambonnet D (1982) Culture in vitro d’anthères d’aubergine (Solanum melongena L.): stimulation de la production de plantes au moyen de traitements Ă 35°C associĂ©s Ă de faibles teneurs en substances de croissance. Agronomie 2:983–988Dunwell JM (2010) Haploids in flowering plants: origins and exploitation. Plant Biotechnol J 8:377–424Eshaghi ZC, Abdollahi MR, Moosavi SS, Deljou A, SeguĂ-Simarro JM (2015) Induction of androgenesis and production of haploid embryos in anther cultures of borage (Borago officinalis L.). Plant Cell Tissue Organ Cult 1–9. doi: 10.1007/s11240-015-0768-5Franklin G, Sheeba CJ, Sita GL (2004) Regeneration of eggplant (Solanum melongena L.) from root explants. In Vitro Cell Dev Biol Plant 40:188–191Gisbert C, Prohens J, Nuez F (2006) Efficient regeneration in two potential new crops for subtropical climates, the scarlet (Solanum aethiopicum) and gboma (S. macrocarpon) eggplants. New Zeal J Crop Hort Sci 34:55–62Kaur M, Dhatt AS, Sandhu JS, Gosal SS (2011) In vitro plant regeneration in brinjal from cultured seedling explants. Indian J Hortic 68:61–65Kim M, Park E-J, An D, Lee Y (2013) High-quality embryo production and plant regeneration using a two-step culture system in isolated microspore cultures of hot pepper (Capsicum annuum L.). Plant Cell Tissue Organ Cult 112:191–201Miyoshi K (1996) Callus induction and plantlet formation through culture of isolated microspores of eggplant (Solanum melongena L). Plant Cell Rep 15:391–395Mohinuddin AKM, Chowdhury MKU, Abdullah Zaliha C, Napis S (1997) Influence of silver nitrate (ethylene inhibitor) on cucumber in vitro shoot regeneration. Plant Cell Tissue Organ Cult 51:75–78Moshkov IE, Novikova GV, Hall MA, George EF (2008) Plant growth regulators III: gibberellins, ethylene, abscisic acid, their analogues and inhibitors; miscellaneous compounds. In George EF, Hall MA, De Klerk GJ (eds) Plant propagation by tissue culture, 3 edn, vol 1. Springer, DordrechtParra-Vega V, Renau-Morata B, Sifres A, SeguĂ-Simarro JM (2013) Stress treatments and in vitro culture conditions influence microspore embryogenesis and growth of callus from anther walls of sweet pepper (Capsicum annuum L.). Plant Cell Tissue Organ Cult 112:353–360Rotino GL (1996) Haploidy in eggplant. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol 3. Kluwer, Dordrecht, pp 115–141Salas P, Prohens J, SeguĂ-Simarro JM (2011) Evaluation of androgenic competence through anther culture in common eggplant and related species. Euphytica 182:261–274SeguĂ-Simarro JM (2015) Androgenesis in solanaceae. In GermanĂ MA, Lambardi M (eds), In vitro embryogenesis. Springer Science + Business Media, The NetherlandsSeguĂ-Simarro JM, Nuez F (2006) Androgenesis induction from tomato anther cultures: callus characterization. Acta Hort 725:855–861SeguĂ-Simarro JM, Corral-MartĂnez P, Parra-Vega V, González-GarcĂa B (2011) Androgenesis in recalcitrant solanaceous crops. Plant Cell Rep 30:765–778Sgamma T, Thomas B, Muleo R (2015) Ethylene inhibitor silver nitrate enhances regeneration and genetic transformation of Prunus avium (L.) cv Stella. Plant Cell Tissue Organ Cult 120:79–88Shivaraj G, Rao S (2011) Rapid and efficient plant regeneration of eggplant (Solanum melongena L.) from cotyledonary leaf explants. Indian J Biotechnol 10:125–129Tuberosa R, Sanghineti MC, Conti S (1987) Anther culture of eggplant Solanum melongena L. lines and hybrids. GenĂ©tica Agrária 41:267–274Veen H, van de Geijn S (1978) Mobility and ionic form of silver as related to longevity of cut carnations. Planta 140:93–96Xing Y, Yu Y, Luo X, Zhang JN, Zhao B, Guo YD (2010) High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena. Biol Plant 54:231–236Zhang P, Phansiri S, Puonti-Kaerlas J (2001) Improvement of cassava shoot organogenesis by the use of silver nitrate in vitro. Plant Cell Tissue Organ Cult 67:47–5
Just-In-Time eTraining Applied To Emergency Medical Services
While the applications of just-in-time training are more and
more spread, the ubiquitous mobile technology has not found
practical uses of this training strategy. As an original example
of services for healthcare, we present in this work an
application of eTraining that makes use of mobile telephones
to transmit medical and on-site information content to
emergency medical personnel that attend and emergency. The
state-of-the-art in related technologies, overall architecture, and
functioning of JITTER (for Just-In-Time Training for
Emergency Responders) is described in this work.Universidad de Málaga. Campus de Excelencia Internacional AndalucĂa Tech. This work has been funded by the FIT-350100-2006-400
PROFIT project of the Spanish Ministerio de Industria,
Turismo y Comercio, American NSF grant DMI-0239180,
NIEHS (National Institute for Environmental Health
Sciences) grant 1R41ES014793-01, BanDeMar Networks,
Inc., the healthcare company iSOFT Sanidad, S.A., and the
CITIC Technology Centre
Effects of growth conditions of donor plants and in vitro culture environment in the viability and the embryogenic response of microspores of different eggplant genotypes
[EN] Notwithstanding the importance of eggplant in global horticulture, doubled haploid production in this species is still far from being efficient. Although acknowledged to have a role in the efficiency of androgenesis induction, factors such as the growth conditions of donor plant or the in vitro culture environment have not been deeply explored or not explored at all in eggplant, which leaves room for further improvement. In this work, we investigated the effects of different in vivo and in vitro parameters on the androgenic performance of different eggplant genotypes, including two hybrids and a DH line. The in vivo parameters included the exposure of donor plants to different temperature and light conditions and to increased levels of boron. The in vitro parameters included the use of different concentrations of NLN medium components, sucrose and growth regulators, and the suspension of microspores at different densities. Our results showed that whereas greenhouse temperature variations or boron application did not to have a positive influence, greenhouse lighting influenced their viability, thereby conditioning the embryogenic response. Changes in different sucrose, salts and hormone levels had different effects in the genotypes studied, which correlated with their genetic constitution. Finally, we determined the best microspore density, different from that previously proposed. Our work shed light on the role of different factors involved in eggplant microspore cultures, some of them not yet studied, contributing to make microspore culture a more efficient tool in eggplant breeding.This work was supported by Grant AGL2017-88135-R to JMSS from Spanish MICINN, respectively, jointly funded by FEDER. ARS and CCF were supported by predoctoral fellowships from the FPI Programs of Universitat Politecnica de Valencia and Generalitat Valenciana, respectively.Rivas-Sendra, A.; Corral MartĂnez, P.; Camacho-Fernández, C.; Porcel, R.; SeguĂ-Simarro, JM. (2020). Effects of growth conditions of donor plants and in vitro culture environment in the viability and the embryogenic response of microspores of different eggplant genotypes. Euphytica. 216(11):1-15. https://doi.org/10.1007/s10681-020-02709-4S11521611Abdollahi MR, Corral-Martinez P, Mousavi A, Salmanian AH, Moieni A, SeguĂ-Simarro JM (2009) An efficient method for transformation of pre-androgenic, isolated Brassica napus microspores involving microprojectile bombardment and Agrobacterium-mediated transformation. Acta Physiol Plant 31:1313–1317Aulinger IE (2002) Combination of in vitro androgenesis and biolistic transformation: an approach for breeding transgenic maize (Zea mays L.) lines. Swiss Federal Institute of Technology, Zurich, p 115Borderies G, le Bechec M, Rossignol M, Lafitte C, Le Deunff E, Beckert M, Dumas C, Matthys-Rochon E (2004) Characterization of proteins secreted during maize microspore culture: arabinogalactan proteins (AGPs) stimulate embryo development. Eur J Cell Biol 83:205–212Bueno MA, GĂłmez A, SepĂşlveda F, SeguĂ-Simarro JM, Testillano PS, Manzanera JA, Risueño MC (2003) Microspore-derived embryos from Quercus suber anthers mimic zygotic embryos and maintain haploidy in long-term anther culture. J Plant Physiol 160:953–960Camacho-Fernández C, Hervás D, Rivas-Sendra A, MarĂn MP, SeguĂ-Simarro JM (2018) Comparison of six different methods to calculate cell densities. Plant Methods 14:30Chambonnet D (1988) Production of haploid eggplant plants. Bulletin interne de la Station d’AmĂ©lioration des Plantes Maraichères d’Avignon-Montfavet, France, pp 1–10Corral-MartĂnez P, SeguĂ-Simarro JM (2012) Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.). Euphytica 187:47–61Corral-MartĂnez P, SeguĂ-Simarro JM (2014) Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins. Euphytica 195:369–382Custers J (2003) Microspore culture in rapeseed (Brassica napus L.). In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. Kluwer Academic Publishers, Dordrecht, pp 185–193Dunwell JM (1976) A comparative study of environmental and developmental factors which influence embryo induction and growth in cultured anthers of Nicotiana tabacum. Environ Exp Bot 16:109–118Dunwell JM (2010) Haploids in flowering plants: origins and exploitation. Plant Biotechnol J 8:377–424Dutta SS, Pale G, Pattanayak A, Aochen C, Pandey A, Rai M (2017) Effect of low light intensity on key traits and genotypes of hilly rice (Oryza sativa) germplasm. J Exp Biol Agric Sci 5:463–471Esteves P, Clermont I, Marchand S, Belzile F (2014) Improving the efficiency of isolated microspore culture in six-row spring barley: II-exploring novel growth regulators to maximize embryogenesis and reduce albinism. Plant Cell Rep 33:871–879 (in press)Gaillard A, Vergne P, Beckerte M (1991) Optimization of maize microspore isolation and culture conditions for reliable plant regeneration. Plant Cell Rep 10:55–58Höfer M (2004) In vitro androgenesis in apple—improvement of the induction phase. Plant Cell Rep 22:365–370Jouannic S, Champion A, SeguĂ-Simarro JM, Salimova E, Picaud A, Tregear J, Testillano P, Risueno MC, Simanis V, Kreis M, Henry Y (2001) The protein kinases AtMAP3Kepsilon1 and BnMAP3Kepsilon1 are functional homologues of S. pombe cdc7p and may be involved in cell division. Plant J 26:637–649Kim M, Jang I-C, Kim J-A, Park E-J, Yoon M, Lee Y (2008) Embryogenesis and plant regeneration of hot pepper (Capsicum annuum L.) through isolated microspore culture. Plant Cell Rep 27:425–434Kim M, Park E-J, An D, Lee Y (2013) High-quality embryo production and plant regeneration using a two-step culture system in isolated microspore cultures of hot pepper (Capsicum annuum L.). Plant Cell Tissue Organ Cult 112:191–201Lantos C, Juhasz AG, Vagi P, Mihaly R, Kristof Z, Pauk J (2012) Androgenesis induction in microspore culture of sweet pepper (Capsicum annuum L.). Plant Biotechnol Rep 6:123–132Liu L, Huang L, Li Y (2013) Influence of boric acid and sucrose on the germination and growth of areca pollen. Am J Plant Sci 4:1669–1674Miyoshi K (1996) Callus induction and plantlet formation through culture of isolated microspores of eggplant (Solanum melongena L). Plant Cell Rep 15:391–395Paire A, Devaux P, Lafitte C, Dumas C, Matthys-Rochon E (2003) Proteins produced by barley microspores and their derived androgenic structures promote in vitro zygotic maize embryo formation. Plant Cell Tissue Organ Cult 73:167–176Parra-Vega V, SeguĂ-Simarro JM (2013) Improvement of an isolated microspore culture protocol for Spanish sweet pepper (Capsicum annuum L.). In: Lanteri S, Rotino GL (eds) Breakthroughs in the genetics and breeding of Capsicum and Eggplant. Universita degli Studi di Torino, Torino, Italy, pp 161–168Peñaloza P, Toloza P (2018) Boron increases pollen quality, pollination, and fertility of different genetic lines of pepper. J Plant Nutr 41:969–979Rivas-Sendra A, Corral-MartĂnez P, Camacho-Fernández C, SeguĂ-Simarro JM (2015) Improved regeneration of eggplant doubled haploids from microspore-derived calli through organogenesis. Plant Cell Tissue Organ Cult 122:759–765Rivas-Sendra A, Calabuig-Serna A, SeguĂ-Simarro JM (2017a) Dynamics of calcium during in vitro microspore embryogenesis and in vivo microspore development in Brassica napus and Solanum melongena. Front Plant Sci 8:1177Rivas-Sendra A, Campos-Vega M, Calabuig-Serna A, SeguĂ-Simarro JM (2017b) Development and characterization of an eggplant (Solanum melongena) doubled haploid population and a doubled haploid line with high androgenic response. Euphytica 213:89Rivas-Sendra A, Corral-MartĂnez P, Porcel R, Camacho-Fernández C, Calabuig-Serna A, SeguĂ-Simarro JM (2019) Embryogenic competence of microspores is associated with their ability to form a callosic, osmoprotective subintinal layer. J Exp Bot 70:1267–1281Robert HS, Grunewald W, Sauer M, Cannoot B, Soriano M, Swarup R, Weijers D, Bennett M, Boutilier K, Friml J (2015) Plant embryogenesis requires AUX/LAX-mediated auxin influx. Development 142:702–711Rotino GL (1996) Haploidy in eggplant. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants. Kluwer Academic Publishers, Dordrecht, pp 115–141Salas P, Prohens J, SeguĂ-Simarro JM (2011) Evaluation of androgenic competence through anther culture in common eggplant and related species. Euphytica 182:261–274Salas P, Rivas-Sendra A, Prohens J, SeguĂ-Simarro JM (2012) Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures. Euphytica 184:235–250Satpute G, Long H, SeguĂ-Simarro JM, Risueño MC, Testillano PS (2005) Cell architecture during gametophytic and embryogenic microspore development in Brassica napus. Acta Physiol Plant 27:665–674Saxena N, Johansen C (1987) Adaptation of chickpea and pigeonpea to abiotic stresses. Proceedings of the consultants’ workshop held at ICRISAT Center, India, 19–21 December 1984, ICRISAT, Patancheru, IndiaSeguĂ-Simarro JM (2010) Androgenesis revisited. Bot Rev 76:377–404SeguĂ-Simarro JM (2016) Androgenesis in solanaceae. In: GermanĂ MA, Lambardi M (eds) In vitro embryogenesis. Springer, New York, pp 209–244SeguĂ-Simarro JM, Nuez F (2008) How microspores transform into haploid embryos: changes associated with embryogenesis induction and microspore-derived embryogenesis. Physiol Plant 134:1–12SeguĂ-Simarro JM, Corral-MartĂnez P, Parra-Vega V, González-GarcĂa B (2011) Androgenesis in recalcitrant solanaceous crops. Plant Cell Rep 30:765–778Sinha R, Eudes F (2015) Dimethyl tyrosine conjugated peptide prevents oxidative damage and death of triticale and wheat microspores. Plant Cell Tissue Organ Cult 122(1):227–237Supena EDJ, Suharsono S, Jacobsen E, Custers JBM (2006) Successful development of a shed-microspore culture protocol for doubled haploid production in Indonesian hot pepper (Capsicum annuum L.). Plant Cell Rep 25:1–10Touraev A, Heberle-Bors E (2003) Anther and microspore culture in tobacco. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. Kluwer Academic Publishers, Dordrecht, pp 223–228Touraev A, Ilham A, Vicente O, Heberle-Bors E (1996a) Stress-induced microspore embryogenesis in tobacco: an optimized system for molecular studies. Plant Cell Rep 15:561–565Touraev A, Indrianto A, Wratschko I, Vicente O, Heberle-Bors E (1996b) Efficient microspore embryogenesis in wheat (Triticum aestivum L.) induced by starvation at high temperatures. Sex Plant Reprod 9:209–215Tsay H-S (1981) Effects of nitrogen supply to donor plants on pollen embryogenesis in cultured tobacco anthers. J Agric Res China 30:5–13Tsay H-S (1982) Microspore development and haploid embryogenesis of anther culture with five nitrogen doses to the donor tobacco plants. J Agric Res China 31:1–13Tuberosa R, Sanguineti MC, Toni B, Cioni F (1987) Ottenimento di aploidi in melanzana (Solanum melongena L.) mediante coltura di antere. Sementi Elette 3:9–14Ĺ»ur I, Dubas E, Krzewska M, Janowiak F (2015) Current insights into hormonal regulation of microspore embryogenesis. Front Plant Sci 6:42
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