Parasitología interactiva: Protozoos y afines

Desarrollo de una guía interactiva donde se muestran los ciclos biológicos, imágenes y dibujos de los estadios evolutivos de los principales géneros y especies de parásitos protozoos relevantes en al ámbito veterinario

Transferencia universidad-secundaria: las TAC y los recursos multimedia generadores de aprendizaje y habilidades en los estudios de la lengua española, lenguas extranjeras y sus literaturas

Memoria ID-043. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2021-2022

Parasitología Interactiva

Desarrollo de una guía interactiva donde se muestran los ciclos biológicos,imágenes y dibujos de los estadios evolutivos de los principales géneros y especies de parásitos protozoos relevantes en al ámbito veterinario

Safety and preliminary efficacy on cognitive performance and adaptive functionality of epigallocatechin gallate (EGCG) in children with Down syndrome. A randomized phase Ib clinical trial (PERSEUS study)

Purpose: Although some caregivers are using epigallocatechin gallate (EGCG) off label in hopes of improving cognition in young adults with Down syndrome (DS), nothing is known about its safety, tolerability, and efficacy in the DS pediatric population. We aimed to evaluate safety and tolerability of a dietary supplement containing EGCG and if EGCG improves cognitive and functional performance. Methods: A total of 73 children with DS (aged 6-12 years) were randomized. Participants received 0.5% EGCG (10 mg/kg daily dose) or placebo for 6 months with 3 months follow up after treatment discontinuation. Results: In total, 72 children were treated and 66 completed the study. A total of 38 participants were included in the EGCG group and 35 in the placebo group. Of 72 treated participants, 62 (86%) had 229 treatment-emergent adverse events (AEs). Of 37 participants in the EGCG group, 13 (35%) had 18 drug-related treatment-emergent AEs and 12 of 35 (34%) from the placebo group had 22 events. In the EGCG group, neither severe AEs nor increase in the incidence of AEs related to safety biomarkers were observed. Cognition and functionality were not improved compared with placebo. Secondary efficacy outcomes in girls point to a need for future work. Conclusion: The use of EGCG is safe and well-tolerated in children with DS, but efficacy results do not support its use in this population. (C) 2022 The Authors. Published by Elsevier Inc. on behalf of American College of Medical Genetics and Genomics

Newer generations of multi-target CAR and STAb-T immunotherapeutics: NEXT CART Consortium as a cooperative effort to overcome current limitations

Adoptive T cellular immunotherapies have emerged as relevant approaches for treating cancer patients who have relapsed or become refractory (R/R) to traditional cancer treatments. Chimeric antigen receptor (CAR) T-cell therapy has improved survival in various hematological malignancies. However, significant limitations still impede the widespread adoption of these therapies in most cancers. To advance in this field, six research groups have created the “NEXT Generation CART MAD Consortium” (NEXT CART) in Madrid’s Community, which aims to develop novel cell-based immunotherapies for R/R and poor prognosis cancers. At NEXT CART, various basic and translational research groups and hospitals in Madrid concur to share and synergize their basic expertise in immunotherapy, gene therapy, and immunological synapse, and clinical expertise in pediatric and adult oncology. NEXT CART goal is to develop new cell engineering approaches and treatments for R/R adult and pediatric neoplasms to evaluate in multicenter clinical trials. Here, we discuss the current limitations of T cell-based therapies and introduce our perspective on future developments. Advancement opportunities include developing allogeneic products, optimizing CAR signaling domains, combining cellular immunotherapies, multi-targeting strategies, and improving tumor-infiltrating lymphocytes (TILs)/T cell receptor (TCR) therapy. Furthermore, basic studies aim to identify novel tumor targets, tumor molecules in the tumor microenvironment that impact CAR efficacy, and strategies to enhance the efficiency of the immunological synapse between immune and tumor cells. Our perspective of current cellular immunotherapy underscores the potential of these treatments while acknowledging the existing hurdles that demand innovative solutions to develop their potential for cancer treatment fully

Exoesqueletos portables en personas con lesión medular. Revisión sistemática

1) Antecedentes: existe gran cantidad de dispositivos para facilitar la bipedestación y la marcha. Cada exoesqueleto tiene características propias, beneficios y limitaciones. Esta revisión compendia y ordena el conocimiento disponible sobre los diferentes tipos de exoesqueletos portables para personas con lesión medular.2) Métodos: se realizó una búsqueda sistemática en Pubmed, Cochrane Libraryy Physiotherapy Evidence Database, sin limitación de tipo de artículo. Además,se analizaron los artículos que figuraban en su bibliografía y se consultaron páginas web relacionadas.3) Resultados: de un total de 128 estudios se seleccionaron 28. Se recogieron datos sobre el nombre del dispositivo y empresa distribuidora, población diana, articulaciones diana, nivel lesional, uso indicado, tipo de terapia, necesidad de ayudas técnicas, autonomía y comercialización.4) Conclusiones: se han encontrado20 exoesqueletos portables que se utilizan por personas con lesión medular.La mayor parte de articulaciones diana son la cadera y la rodilla. La mayoría se emplean con fines rehabilitadores y necesitan un producto deapoyo complementario1) Background: there are many devices to facilitate standing and walking. Each exoskeleton has its characteristics, benefits, and limitations. This review summarizes and orders the available knowledge about the different types of portable exoskeletons for people with spinal cord injury. 2) Methods: a systematic search was conducted in Pubmed, Cochrane Library, and Physiotherapy Evidence Database, without limitation of the type of article. Also, the articles in their bibliography were analyzed, and related web pages were consulted. 3) Results: from a total of 128 studies, 28 were selected. Data were collected on the name of the device and distribution company, target population, target joints, injury level, indicated use, type of therapy, need for technical assistance, autonomy, and commercialization. 4) Conclusions: 20 portable exoskeletons have been found that are used by people with spinal cord injury. Most of the target joints are the hip and the knee. Most are used for rehabilitation purposes and need a complementary support product

Aproximación a la lectura y a la escritura : 0-3 años; niños-as sordos y oyentes

Proyecto que pretende iniciar a los niños, oyentes y sordos, en la lectura y escritura. Sus objetivos son que los niños entren en el mundo de la palabra, de los mensajes escritos, leyendo las frases que acompañan a cuentos, objetos y otros materiales; que se familiaricen con diferentes textos, acostumbrándoles a su presencia en el aula, en la escuela y en casa; y que los niños comiencen a utilizar la lectura y la escritura como dos herramientas habituales en la vida cotidiana, y sobre todo en la escuela. La metodología sigue el principio del aprendizaje significativo, dentro de un marco lúdico, cotidiano y contextualizado a la vida de cada uno de los grupos de niños. Las actividades que se realizan son la Semana del Cuento, una sesión en la Escuela de Padres para las familias de los niños sordos, y la casa de Don Libro y Lapicerín. La evaluación se realiza a través de fichas de observación de cada niño, y mediante la autoevaluación de los educadores. Se elaboran diversos materiales, como cuentos, documentación para las familias, diapositivas de cuentos y de imágenes, libros de imágenes y sensoriales, mascotas Don Libro y Lapicerín, la casita de periódico de las dos mascotas, y material para la zona de la biblioteca y de juego simbólico.Madrid (Comunidad Autónoma). Consejería de EducaciónMadridMadrid (Comunidad Autónoma). Subdirección General de Formación del Profesorado. CRIF Las Acacias; General Ricardos 179 - 28025 Madrid; Tel. + 34915250893ES

Proteomic investigations into hypertension: What’s new and how might it affect clinical practice?

Introduction: Hypertension is a multifactorial disease that has, thus far, proven to be a difficult target for pharmacological intervention. The application of proteomic strategies may help to identify new biomarkers for the early diagnosis and prompt treatment of hypertension, in order to control blood pressure and prevent organ damage. Areas covered: Advances in proteomics have led to the discovery of new biomarkers to help track the pathophysiological processes implicated in hypertension. These findings not only help to better understand the nature of the disease, but will also contribute to the clinical needs for a timely diagnosis and more precise treatment. In this review, we provide an overview of new biomarkers identified in hypertension through the application of proteomic techniques, and we also discuss the difficulties and challenges in identifying biomarkers in this clinical setting. We performed a literature search in PubMed with the key words ‘hypertension’ and ‘proteomics’, and focused specifically on the most recent literature on the utility of proteomics in hypertension research. Expert opinion: There have been several promising biomarkers of hypertension identified by proteomics, but too few have been introduced to the clinic. Thus, further investigations in larger cohorts are necessary to test the feasibility of this strategy for patients. Also, this emerging field would profit from more collaboration between clinicians and researchers.Sin financiación3.614 JCR (2019) Q1, 19/77 Biochemical Research Methods0.979 SJR (2019) Q2, 155/456 Biochemistry; Q3, 206/414 Molecular BiologyNo data IDR 2019UE

Approach to amoebic colitis : Epidemiological, clinical and diagnostic considerations in a non-endemic context (Barcelona, 2007-2017)

Background Amoebic colitis is the most frequent clinical manifestation of invasive intestinal infection due to Entamoeba histolytica and a common cause of diarrhoea worldwide. Since higher transmission rates are usually related to poor health and exposure to unhygienic conditions, cases reported in Europe usually involve immigrants and international travellers. The goal of this study was to characterise both the clinical and the epidemiological features of a European population diagnosed with amoebic colitis and then to evaluate the diagnostic tools and therapeutic options applied. Methods and results This was a retrospective observational study in which data from all patients diagnosed with amoebic colitis attending at the International Health Units of two tertiary referral hospitals, Germans Trias i Pujol University Hospital (Badalona, North Barcelona Metropolitan Area) and Vall d'Hebron University Hospital (Barcelona city) between 2007 and 2017 were analysed. During the study period 50 patients were diagnosed with amoebic colitis. Thirty-six (72%) were men, and immigrants accounted for 46% of all cases. Antecedents of any international travel were reported for 28 (56%), the most frequent destinations having been the Indian subcontinent, South and Central America and sub-Saharan Africa. Preexisting pathological conditions or any kind of immunosuppression were identified in 29 (58%) patients; of these, 13 (26%) had HIV infection-all of them men who have sex with men-and 5 (10%) had inflammatory bowel disease. Diarrhoea, abdominal pain and dysentery were the most frequently recorded symptoms of invasive amoebae. Diagnosis was made through microbiological study in 45 (90%) and/or histological identification of amoebae in colon biopsies in 10 (20%). After treatment with metronidazole (82%) or tinidazole (8%), all patients had good outcomes. Post-acute intraluminal treatment was indicated in 28 (56%). Conclusions Amoebic colitis should be suspected in patients with diarrhoea and compatible epidemiological risk factors (immigration, travelling abroad or men who have sex with men), especially if some degree of immunosuppression concurs. These risk factors must be taken into account in any diagnostic approach to inflammatory bowel disease (IBD), and active searches for stool parasites should be performed in such cases to rule out misdiagnosis or simultaneous amoebic infection. Treatment should include intraluminal anti-amoebic treatment in order to avoid relapse and prevent further spread of the disease

Endogenous auxin accumulation/localization during zygotic and somatic embryogenesis of Capsicum chinense Jacq

37 p.-9 fig.Zygotic and somatic embryogenesis in plants is a fascinating event that is finely regulated through the expression of a specific group of genes and dynamic levels of plant hormones whose concerted action determines the fate that specific cells follow towards zygotic or somatic embryo development. This work studied different stages of Capsicum chinense Jacq. zygotic and somatic embryogenesis. HPLC quantification determined that the levels of indole-3-acetic acid (IAA) increase as the zygotic or somatic embryogenesis progresses, being higher at maturity, thus supporting a positive correlation between embryo cell differentiation and IAA increase. A monoclonal anti-IAA-antibody was used to detect IAA levels. Findings revealed a dynamic pattern of auxin distribution along the different embryogenic embryonic stages. In the early stages of zygotic embryos, the IAA gradient was observed in the basal cells of the suspensor and the hypostases, suggesting that they are the initial source of the IAA hormone. As embryogenesis proceeds, the dynamic of the IAA gradient is displaced to the embryo and endosperm cells. In the case of induced somatic embryogenesis, the IAA gradient was detected in the dividing cells of the endodermis, from where pre-embryogenic cells emerge. However, the analysis of somatic embryos revealed that IAA was homogeneously distributed. This study shows evidence supporting a correlation between IAA levels during zygotic or somatic embryogenesis in Capsicum chinense species.This manuscript received financing from CONACYT-México through the project with reference number PDCPN2016 3953. This study was funded by Project grant AGL2017-82447-R, funded by Spanish AEI and European ERDF/FEDER too.Peer reviewe