Supporting Education, Research, and Training in the 21st Century

The expanding array of digital devices accessing the Internet around the globe provides new opportunities to collaborate in educating Adventist teachers and educational leaders to continue the teaching ministry of Jesus Christ in the 21st century. Partnerships among the Curriculum and Instruction Resource Center Linking Educators (CIRCLE), the Adventist Learning Community (ALC), and the Institute of Church Ministry (ICM) now connect more Adventists to education and training resources electronically than ever before

Exploration of the Age-Category Soccer Performance Effects During Ball Possession Small-Sided Games

Small-sided games have been adopted as an integral part of soccer training, however, the use of task constraints by the coach and the action capabilities of both players and teams require further investigation. The aim of this investigation was to explore the age-category effects (under-11: U11, under-15: U15 and under-23: U23) on external training workloads (total distance covered, distance covered while walking, running and sprinting, number of sprints and maximum sprint speed), internal training load metrics (rate of perceived exertion, RPE) and tactical individual actions (passing number with dominant and non-dominant foot, and max passing speed) during 4 vs. 4 ball possession small-sided game constrained within three different playing areas (small: 20 x 15 m, medium: 25 x 20 m, and large: 30 x 25 m). Results revealed substantial differences (all p < .001) for each specific playing area observed across many of the external workload measures. For every area analysed, U23 players covered more distance walking, whereas U11 and U15 players covered more distances at higher intensities. Additionally, significant differences were found for the RPE (small playing area: p = .001; large playing area: p < .001) with U23 and U15 players showing higher scores compared with U11 ones. It can be concluded that a 4 vs. 4 ball possession small-sided game can provide different performance related stimuli to players, depending on age category and the playing surface area. Therefore, coaches and individuals involved with training and development of soccer players across all age groups should be aware of the key variables highlighted in this study before planning training drills

Leaf application of manganese in genetically modified soybean submitted to glyphosate levels

O objetivo deste trabalho foi avaliar a influência da aplicação de glyphosate na soja Roundup Ready® e a produtividade em diferentes doses de manganês aplicado via foliar. O experimento foi realizado na safra 2011/12 no município de Nova Maringá – MT com delineamento experimental de blocos ao acaso, em esquema fatorial 3 x 4, com quatro repetições. Foram utilizadas três doses de glyphosate (0,0; 0,972 e 1,620 kg i.a. ha-1) e quatro doses de manganês (0,0; 0,350; 0,700 e 1,050 kg ha-1). O glyphosate foi aplicado no estádio fenológico V2 da cultura e as doses de manganês em estádio V8. Os parâmetros analisados foram altura de planta, altura de inserção da primeira vagem, fitomassa verde, fitomassa seca, número de vagens por planta, massa de 1000 grãos e produtividade de grãos. Para altura de inserção da primeira vagem, altura de plantas, massa de 1000 grãos e produtividade de grãos a aplicação de glyphosate e manganês não ocasionaram efeitos expressivos no desenvolvimento da cultivar de soja TMG 133 RR. Os tratamentos com glyphosate e manganês e a interação deles não proporcionaram incrementos de produtividade de grãos na cultivar TMG 133 RR.The aim of this study was to evaluate glyphosate application in Roundup Ready® soybean and yield at different levels of manganese applied by foliar way. The experiment was conducted in 2011/2012 agricultural year in Nova Maringa County, Mato Grosso State, in randomized blocks experimental design, in factorial scheme 3 x 4, with four replications. Three glyphosate levels (0.0; 0.972; 1.620 kg a.i. ha-1) and four manganese levels (0.0; 0.350; 0.700 and 1.050 kg ha-1) were used. Glyphosate was applied at V2 crop growth stage and manganese levels during V8 crop growth stage. Analyzed parameters were plant height, height of first pod insertion, green mass, dry mass, number of pods per plant, mass of 1,000 grains and yield. For height of first pod insertion, plant height, mass of 1,000 grains and yield, glyphosate and manganese applications did not cause significant effects over TMG 133 RR development. Treatments with glyphosate and manganese and its interaction did not provide increased yield in TMG 133 RR genotype

Ectopic Expression of Vaccinia Virus E3 and K3 Cannot Rescue Ectromelia Virus Replication in Rabbit RK13 Cells

Citation: Hand, E. S., Haller, S. L., Peng, C., Rothenburg, S., & Hersperger, A. R. (2015). Ectopic Expression of Vaccinia Virus E3 and K3 Cannot Rescue Ectromelia Virus Replication in Rabbit RK13 Cells. Plos One, 10(3), 15. doi:10.1371/journal.pone.0119189As a group, poxviruses have been shown to infect a wide variety of animal species. However, there is individual variability in the range of species able to be productively infected. In this study, we observed that ectromelia virus (ECTV) does not replicate efficiently in cultured rabbit RK13 cells. Conversely, vaccinia virus (VACV) replicates well in these cells. Upon infection of RK13 cells, the replication cycle of ECTV is abortive in nature, resulting in a greatly reduced ability to spread among cells in culture. We observed ample levels of early gene expression but reduced detection of virus factories and severely blunted production of enveloped virus at the cell surface. This work focused on two important host range genes, named E3L and K3L, in VACV. Both VACV and ECTV express a functional protein product from the E3L gene, but only VACV contains an intact K3L gene. To better understand the discrepancy in replication capacity of these viruses, we examined the ability of ECTV to replicate in wild-type RK13 cells compared to cells that constitutively express E3 and K3 from VACV. The role these proteins play in the ability of VACV to replicate in RK13 cells was also analyzed to determine their individual contribution to viral replication and PKR activation. Since E3L and K3L are two relevant host range genes, we hypothesized that expression of one or both of them may have a positive impact on the ability of ECTV to replicate in RK13 cells. Using various methods to assess virus growth, we did not detect any significant differences with respect to the replication of ECTV between wild-type RK13 compared to versions of this cell line that stably expressed VACV E3 alone or in combination with K3. Therefore, there remain unanswered questions related to the factors that limit the host range of ECTV

Shaping care home COVID-19 testing policy: a protocol for a pragmatic cluster randomised controlled trial of asymptomatic testing compared with standard care in care home staff (VIVALDI-CT)

INTRODUCTION: Care home residents have experienced significant morbidity, mortality and disruption following outbreaks of SARS-CoV-2. Regular SARS-CoV-2 testing of care home staff was introduced to reduce transmission of infection, but it is unclear whether this remains beneficial. This trial aims to investigate whether use of regular asymptomatic staff testing, alongside funding to reimburse sick pay for those who test positive and meet costs of employing agency staff, is a feasible and effective strategy to reduce COVID-19 impact in care homes. METHODS AND ANALYSIS: The VIVALDI-Clinical Trial is a multicentre, open-label, cluster randomised controlled, phase III/IV superiority trial in up to 280 residential and/or nursing homes in England providing care to adults aged >65 years. All regular and agency staff will be enrolled, excepting those who opt out. Homes will be randomised to the intervention arm (twice weekly asymptomatic staff testing for SARS-CoV-2) or the control arm (current national testing guidance). Staff who test positive for SARS-CoV-2 will self-isolate and receive sick pay. Care providers will be reimbursed for costs associated with employing temporary staff to backfill for absence arising directly from the trial.The trial will be delivered by a multidisciplinary research team through a series of five work packages.The primary outcome is the incidence of COVID-19-related hospital admissions in residents. Secondary outcomes include the number and duration of outbreaks and home closures. Health economic and modelling analyses will investigate the cost-effectiveness and cost consequences of the testing intervention. A process evaluation using qualitative interviews will be conducted to understand intervention roll out and identify areas for optimisation to inform future intervention scale-up, should the testing approach prove effective and cost-effective. Stakeholder engagement will be undertaken to enable the sector to plan for results and their implications and to coproduce recommendations on the use of testing for policy-makers. ETHICS AND DISSEMINATION: The study has been approved by the London-Bromley Research Ethics Committee (reference number 22/LO/0846) and the Health Research Authority (22/CAG/0165). The results of the trial will be disseminated regardless of the direction of effect. The publication of the results will comply with a trial-specific publication policy and will include submission to open access journals. A lay summary of the results will also be produced to disseminate the results to participants. TRIAL REGISTRATION NUMBER: ISRCTN13296529

Shaping care home COVID-19 testing policy: a protocol for a pragmatic cluster randomised controlled trial of asymptomatic testing compared with standard care in care home staff (VIVALDI-CT)

INTRODUCTION: Care home residents have experienced significant morbidity, mortality and disruption following outbreaks of SARS-CoV-2. Regular SARS-CoV-2 testing of care home staff was introduced to reduce transmission of infection, but it is unclear whether this remains beneficial. This trial aims to investigate whether use of regular asymptomatic staff testing, alongside funding to reimburse sick pay for those who test positive and meet costs of employing agency staff, is a feasible and effective strategy to reduce COVID-19 impact in care homes. METHODS AND ANALYSIS: The VIVALDI-Clinical Trial is a multicentre, open-label, cluster randomised controlled, phase III/IV superiority trial in up to 280 residential and/or nursing homes in England providing care to adults aged >65 years. All regular and agency staff will be enrolled, excepting those who opt out. Homes will be randomised to the intervention arm (twice weekly asymptomatic staff testing for SARS-CoV-2) or the control arm (current national testing guidance). Staff who test positive for SARS-CoV-2 will self-isolate and receive sick pay. Care providers will be reimbursed for costs associated with employing temporary staff to backfill for absence arising directly from the trial.The trial will be delivered by a multidisciplinary research team through a series of five work packages.The primary outcome is the incidence of COVID-19-related hospital admissions in residents. Secondary outcomes include the number and duration of outbreaks and home closures. Health economic and modelling analyses will investigate the cost-effectiveness and cost consequences of the testing intervention. A process evaluation using qualitative interviews will be conducted to understand intervention roll out and identify areas for optimisation to inform future intervention scale-up, should the testing approach prove effective and cost-effective. Stakeholder engagement will be undertaken to enable the sector to plan for results and their implications and to coproduce recommendations on the use of testing for policy-makers. ETHICS AND DISSEMINATION: The study has been approved by the London-Bromley Research Ethics Committee (reference number 22/LO/0846) and the Health Research Authority (22/CAG/0165). The results of the trial will be disseminated regardless of the direction of effect. The publication of the results will comply with a trial-specific publication policy and will include submission to open access journals. A lay summary of the results will also be produced to disseminate the results to participants. TRIAL REGISTRATION NUMBER: ISRCTN13296529

Effects of varying organic matter content on the development of green roof vegetation: a six year experiment

Green roofs can potentially be used to tackle a variety of environmental problems, and can be used as development mitigation for the loss of ground-based habitats. Brown (biodiversity) roofs are a type of green roof designed to imitate brownfield habitat, but the best way of engineering these habitats requires more research. We tested the effects of altering organic matter content on the development of vegetation assemblages of experimental brown (biodiversity) roof mesocosms. Three mulch treatments were tested: (1) Sandy loam, where 10mm of sandy loam mulch (about 3% organic matter by dry weight) was added to 100mm of recycled aggregate; (2) Compost, where the mulch also contained some garden compost (about 6% organic matter by dry weight); and (3) No mulch, where no mulch was added. Mesocosms were seeded with a wildflower mix that included some Sedum acre, and vegetation development was investigated over a six-year period. Species richness, assemblage character, number of plants able to seed, and above-ground plant biomass were measured. Drought disturbance was an important control on plant assemblages in all mulch treatments, but there were significant treatment response interactions. The more productive Compost treatment was associated with larger plant coverage and diversity before the occurrence of a sequence of drought disturbances, but was more strongly negatively affected by the disturbances than the two less productive treatments. We suggest that this was due to the over-production of plant biomass in the more productive treatment, which made the plants more vulnerable to the effects of drought disturbance, leading to a kind of 'boom-bust' assemblage dynamic. The 'ideal' amount of added organic matter for these green roof systems was very low, but other types of green roof that have a larger water holding capacity, and/or more drought resistant plant floras, will likely require more organic matter or fertiliser. Nonetheless, nutrient-supported productivity in green roof systems should be kept low in order to avoid boom-bust plant assemblage dynamics. Research into the best way of engineering green roof habitats should take place over a long enough multi-year time period to include the effects of temporally infrequent disturbances

Shaping care home COVID-19 testing policy : a protocol for a pragmatic cluster randomised controlled trial of asymptomatic testing compared with standard care in care home staff (VIVALDI-CT)

Introduction Care home residents have experienced significant morbidity, mortality and disruption following outbreaks of SARS-CoV-2. Regular SARS-CoV-2 testing of care home staff was introduced to reduce transmission of infection, but it is unclear whether this remains beneficial. This trial aims to investigate whether use of regular asymptomatic staff testing, alongside funding to reimburse sick pay for those who test positive and meet costs of employing agency staff, is a feasible and effective strategy to reduce COVID-19 impact in care homes. Methods and analysis The VIVALDI-Clinical Trial is a multicentre, open-label, cluster randomised controlled, phase III/IV superiority trial in up to 280 residential and/or nursing homes in England providing care to adults aged >65 years. All regular and agency staff will be enrolled, excepting those who opt out. Homes will be randomised to the intervention arm (twice weekly asymptomatic staff testing for SARS-CoV-2) or the control arm (current national testing guidance). Staff who test positive for SARS-CoV-2 will self-isolate and receive sick pay. Care providers will be reimbursed for costs associated with employing temporary staff to backfill for absence arising directly from the trial. The trial will be delivered by a multidisciplinary research team through a series of five work packages. The primary outcome is the incidence of COVID-19-related hospital admissions in residents. Secondary outcomes include the number and duration of outbreaks and home closures. Health economic and modelling analyses will investigate the cost-effectiveness and cost consequences of the testing intervention. A process evaluation using qualitative interviews will be conducted to understand intervention roll out and identify areas for optimisation to inform future intervention scale-up, should the testing approach prove effective and cost-effective. Stakeholder engagement will be undertaken to enable the sector to plan for results and their implications and to coproduce recommendations on the use of testing for policy-makers. Ethics and dissemination The study has been approved by the London—Bromley Research Ethics Committee (reference number 22/LO/0846) and the Health Research Authority (22/CAG/0165). The results of the trial will be disseminated regardless of the direction of effect. The publication of the results will comply with a trial-specific publication policy and will include submission to open access journals. A lay summary of the results will also be produced to disseminate the results to participants