A Universal Live Cell Barcoding-Platform for Multiplexed Human Single Cell Analysis.

Single-cell barcoding enables the combined processing and acquisition of multiple individual samples as one. This maximizes assay efficiency and eliminates technical variability in both sample preparation and analysis. Remaining challenges are the barcoding of live, unprocessed cells to increase downstream assay performance combined with the flexibility of the approach towards a broad range of cell types. To that end, we developed a novel antibody-based platform that allows the robust barcoding of live human cells for mass cytometry (CyTOF). By targeting both the MHC class I complex (beta-2-microglobulin) and a broadly expressed sodium-potassium ATPase-subunit (CD298) with platinum-conjugated antibodies, human immune cells, stem cells as well as tumor cells could be multiplexed in the same single-cell assay. In addition, we present a novel palladium-based covalent viability reagent compatible with this barcoding strategy. Altogether, this platform enables mass cytometry-based, live-cell barcoding across a multitude of human sample types and provides a scheme for multiplexed barcoding of human single-cell assays in general

On the Regularized Fermionic Projector of the Vacuum

We construct families of fermionic projectors with spherically symmetric regularization, which satisfy the condition of a distributional ${\mathcal{M}} P$-product. The method is to analyze regularization tails with a power-law or logarithmic scaling in composite expressions in the fermionic projector. The resulting regularizations break the Lorentz symmetry and give rise to a multi-layer structure of the fermionic projector near the light cone. The remaining freedom for the regularization parameters and the consequences for the normalization of the fermionic states are discussed.Comment: 66 pages, LaTeX, 8 figures, minor improvements (published version

Generalized μ−τ\mu-\tau reflection symmetry and leptonic CP violation

We propose a generalized $\mu-\tau$ reflection symmetry to constrain the lepton flavor mixing parameters. We obtain a new correlation between the atmospheric mixing angle $\theta_{23}$ and the "Dirac" CP violation phase $\delta_{\rm CP}$. Only in a specific limit our proposed CP transformation reduces to standard $\mu-\tau$ reflection, for which $\theta_{23}$ and $\delta_{CP}$ are both maximal. The "Majorana" phases are predicted to lie at their CP-conserving values with important implications for the neutrinoless double beta decay rates. We also study the phenomenological implications of our scheme for present and future neutrino oscillation experiments including T2K, NO$\nu$A and DUNE.Comment: 14 pages, 9 figures, latex, Final version to appear in Physics Letters

Analysis of dependence among size, rate and duration in internet flows

In this paper we examine rigorously the evidence for dependence among data size, transfer rate and duration in Internet flows. We emphasize two statistical approaches for studying dependence, including Pearson's correlation coefficient and the extremal dependence analysis method. We apply these methods to large data sets of packet traces from three networks. Our major results show that Pearson's correlation coefficients between size and duration are much smaller than one might expect. We also find that correlation coefficients between size and rate are generally small and can be strongly affected by applying thresholds to size or duration. Based on Transmission Control Protocol connection startup mechanisms, we argue that thresholds on size should be more useful than thresholds on duration in the analysis of correlations. Using extremal dependence analysis, we draw a similar conclusion, finding remarkable independence for extremal values of size and rate.Comment: Published in at http://dx.doi.org/10.1214/09-AOAS268 the Annals of Applied Statistics (http://www.imstat.org/aoas/) by the Institute of Mathematical Statistics (http://www.imstat.org

Non-Existence of Time-Periodic Solutions of the Dirac Equation in a Reissner-Nordstrom Black Hole Background

It is shown analytically that the Dirac equation has no normalizable, time-periodic solutions in a Reissner-Nordstrom black hole background; in particular, there are no static solutions of the Dirac equation in such a background field. The physical interpretation is that Dirac particles can either disappear into the black hole or escape to infinity, but they cannot stay on a periodic orbit around the black hole.Comment: 24 pages, 2 figures (published version

Model Fragmentasi Sistem Basis Data Terdistribusi Studi Kasus Sistem Member Warnet

Sistem terdistribusi merupakan suatu bentuk arsitektur sistem dimana komputer-komputer yangberdiri secara otonom dapat saling berkomunikasi dan berbagi resource tanpa memperdulikan dimanakomputer itu berada dan platform yang digunakan.Bisnis warnet dan game center saat ini dianggap sebagai bisnis yang menggiurkan. Untukmemperluas pasar biasanya digunakan strategi membuka cabang baru dan pemberlakuan program khusus.Sistem member yang diberlakukan memberikan fasilitas potongan harga dibandingkan dengan user yangtidak masuk dalam program member dan prepaid atau prabayar. Dengan sistem tersebut mereka berharapdapat mengikat konsumennya untuk menjadi seorang pelanggan.Pada penelitian ini akan dibahas mengenai penerapan sistem terdistribusi pada sistem memberwarnet. Sehingga member warnet dapat menggunakan keanggotaannya untuk mendapatkan potongan hargapada semua warnet yang dimiliki oleh pengusaha warnet tersebut