A Universal Live Cell Barcoding-Platform for Multiplexed Human Single Cell Analysis.

Single-cell barcoding enables the combined processing and acquisition of multiple individual samples as one. This maximizes assay efficiency and eliminates technical variability in both sample preparation and analysis. Remaining challenges are the barcoding of live, unprocessed cells to increase downstream assay performance combined with the flexibility of the approach towards a broad range of cell types. To that end, we developed a novel antibody-based platform that allows the robust barcoding of live human cells for mass cytometry (CyTOF). By targeting both the MHC class I complex (beta-2-microglobulin) and a broadly expressed sodium-potassium ATPase-subunit (CD298) with platinum-conjugated antibodies, human immune cells, stem cells as well as tumor cells could be multiplexed in the same single-cell assay. In addition, we present a novel palladium-based covalent viability reagent compatible with this barcoding strategy. Altogether, this platform enables mass cytometry-based, live-cell barcoding across a multitude of human sample types and provides a scheme for multiplexed barcoding of human single-cell assays in general

On the Regularized Fermionic Projector of the Vacuum

We construct families of fermionic projectors with spherically symmetric regularization, which satisfy the condition of a distributional ${\mathcal{M}} P$-product. The method is to analyze regularization tails with a power-law or logarithmic scaling in composite expressions in the fermionic projector. The resulting regularizations break the Lorentz symmetry and give rise to a multi-layer structure of the fermionic projector near the light cone. The remaining freedom for the regularization parameters and the consequences for the normalization of the fermionic states are discussed.Comment: 66 pages, LaTeX, 8 figures, minor improvements (published version

Generalized μ−τ\mu-\tau reflection symmetry and leptonic CP violation

We propose a generalized $\mu-\tau$ reflection symmetry to constrain the lepton flavor mixing parameters. We obtain a new correlation between the atmospheric mixing angle $\theta_{23}$ and the "Dirac" CP violation phase $\delta_{\rm CP}$. Only in a specific limit our proposed CP transformation reduces to standard $\mu-\tau$ reflection, for which $\theta_{23}$ and $\delta_{CP}$ are both maximal. The "Majorana" phases are predicted to lie at their CP-conserving values with important implications for the neutrinoless double beta decay rates. We also study the phenomenological implications of our scheme for present and future neutrino oscillation experiments including T2K, NO$\nu$A and DUNE.Comment: 14 pages, 9 figures, latex, Final version to appear in Physics Letters

Analysis of dependence among size, rate and duration in internet flows

In this paper we examine rigorously the evidence for dependence among data size, transfer rate and duration in Internet flows. We emphasize two statistical approaches for studying dependence, including Pearson's correlation coefficient and the extremal dependence analysis method. We apply these methods to large data sets of packet traces from three networks. Our major results show that Pearson's correlation coefficients between size and duration are much smaller than one might expect. We also find that correlation coefficients between size and rate are generally small and can be strongly affected by applying thresholds to size or duration. Based on Transmission Control Protocol connection startup mechanisms, we argue that thresholds on size should be more useful than thresholds on duration in the analysis of correlations. Using extremal dependence analysis, we draw a similar conclusion, finding remarkable independence for extremal values of size and rate.Comment: Published in at http://dx.doi.org/10.1214/09-AOAS268 the Annals of Applied Statistics (http://www.imstat.org/aoas/) by the Institute of Mathematical Statistics (http://www.imstat.org

Non-Existence of Time-Periodic Solutions of the Dirac Equation in a Reissner-Nordstrom Black Hole Background

It is shown analytically that the Dirac equation has no normalizable, time-periodic solutions in a Reissner-Nordstrom black hole background; in particular, there are no static solutions of the Dirac equation in such a background field. The physical interpretation is that Dirac particles can either disappear into the black hole or escape to infinity, but they cannot stay on a periodic orbit around the black hole.Comment: 24 pages, 2 figures (published version

Variação cromossômica numérica em Zephyranthes Herb. (Amaryllidaceae).

O presente trabalho teve como objetivo avaliar a variação cromossômica numérica em duas populações distintas de Zephyranthes, uma no município de Petrolina-PE, e outra em Pocinhos-PB

Low-field microwave absorption and magnetoresistance in iron nanostructures grown by electrodeposition on n-type lightly-doped silicon substrates

In this study we investigate magnetic properties, surface morphology and crystal structure in iron nanoclusters electrodeposited on lightly-doped (100) n-type silicon substrates. Our goal is to investigate the spin injection and detection in the Fe/Si lateral structures. The samples obtained under electric percolation were characterized by magnetoresistive and magnetic resonance measurements with cycling the sweeping applied field in order to understand the spin dynamics in the as-produced samples. The observed hysteresis in the magnetic resonance spectra, plus the presence of a broad peak in the non-saturated regime confirming the low field microwave absorption (LFMA), were correlated to the peaks and slopes found in the magnetoresistance curves. The results suggest long range spin injection and detection in low resistive silicon and the magnetic resonance technique is herein introduced as a promising tool for analysis of electric contactless magnetoresistive samples.Comment: 12 pages, 5 figure

Efek Tipe Kecurangan Dan Anonimitas Terhadap Keputusan Investigasi Auditor Internal Atas Tuduhan Whistleblowing

A number of factors affects the effectiveness of whistleblowing system, such as the whistleblower, the wrongdoer, and other relevant parties. However, whistleblowing literature, so far, seems to attend more on whistleblowers' perspectives. Little is known about aspects from recipient perspectives, especially the effect of psychological biases that can seriously damage the effectiveness of the system. This study extend the whistleblowing literature by experimentally investigating how the fraud type and anonymity influence the recipient decision making process. The results show that internal auditor (as recipient) has lower probability to respond to fraudulent earning management allegation than to that of asset misappropriation. In regard to the anonymity effect, this research is consistent with prior studies in that anonymity provides less credible information for fraud allegation. This study contributes to the literature by highlighting the importance of type of fraud as contextual factor that determine the whistleblowing effectiveness from recipient perspectives