Reinitiation of protein synthesis in Escherichia coli can be induced by mRNA cis-elements unrelated to canonical translation initiation signals

AbstractIn Eubacteria, de novo translation of some internal cistrons may be inefficient or impossible unless the 5′ neighboring cistron is also translated (translational coupling). Translation reinitiation is an extreme case of translational coupling in which translation of a message depends entirely on the presence of a nearby terminating ribosome. In this work, the characteristics of mRNA cis-elements inducing the reinitiation process in Escherichia coli have been investigated using a combinatorial approach. A number of novel translational reinitiation sequences (TRSs) were thus identified, which show a wide range of reinitiation activities fully dependent on a translational coupling event and unrelated to the presence/absence of secondary structure or mRNA stability. Moreover, some of the isolated TRSs are similar to intercistronic sequences present in the E. coli genome

HAPPY MAMA Project (Part 2)-Maternal Distress and Self-Efficacy. A Pilot Randomized Controlled Field Trial

Introduction: The aim of the pilot randomized controlled field trial is to assess if a mid- wifery intervention is able to increase the maternal self-efficacy and reduce the stress level during the first six months after birth. Methods: The study was conducted in two different hospitals in Rome, Italy, involving women delivering at or beyond term, aged >18 years old and with normal APGAR scores of the infant. The participants were randomly divided into two groups: “Individual Intervention Group” (they received home midwifery assistance for one month after birth, I) and the “Control Group” (C). A self-administered questionnaire was administered four times: at the baseline about one week after the hospital delivery (T0), after the intervention about one month after the delivery (T1), and at three months (T2) and at six months after birth (T3). The questionnaire included different validated scales needed to assess maternal perceived self-efficacy (KPCS), parental stress scale stress (PSS) and maternal depressive risk symptoms (EPDS). Results: The study population counted 51 mothers: 28 women in the “C” group and 23 women in the “I” group. The PSS score was statistically higher in the “C” than “I” group at T1 (p = 0.024); whereas the KPCS score was statisti- cally higher in the “I” (p = 0.039) group; EPDS score did not show significant difference between the two groups in the follow-up period. An inverse significant correlation between KPCS and PSS was found during the study window time (p < 0.0001). Conclusions: These results potentially give the opportunity to explore this area of focus further, in order to better address maternal individual needs for the successful transition to motherhood. More research in this area is required

Insight from an Italian Delphi Consensus on EVAR feasibility outside the instruction for use: the SAFE EVAR Study

BACKGROUND: The SAfety and FEasibility of standard EVAR outside the instruction for use (SAFE-EVAR) Study was designed to define the attitude of Italian vascular surgeons towards the use of standard endovascular repair (EVAR) for infrarenal abdominal aortic aneurysm (AAA) outside the instruction for use (IFU) through a Delphi consensus endorsed by the Italian Society of Vascular and Endovascular Surgery (Societa Italiana di Chirurgia Vascolare ed Endovascolare - SICVE). METHODS: A questionnaire consisting of 26 statements was developed, validated by an 18 -member Advisory Board, and then sent to 600 Italian vascular surgeons. The Delphi process was structured in three subsequent rounds which took place between April and June 2023. In the first two rounds, respondents could indicate one of the following five degrees of agreement: 1) strongly agree; 2) partially agree; 3) neither agree nor disagree; 4) partially disagree; 5) strongly disagree; while in the third round only three different choices were proposed: 1) agree; 2) neither agree nor disagree; 3) disagree. We considered the consensus reached when >70% of respondents agreed on one of the options. After the conclusion of each round, a report describing the percentage distribution of the answers was sent to all the participants. RESULTS: Two -hundred -forty-four (40.6%) Italian Vascular Surgeons agreed to participate the first round of the Delphi Consensus; the second and the third rounds of the Delphi collected 230 responders (94.3% of the first -round responders). Four statements (15.4%) reached a consensus in the first rounds. Among the 22 remaining statements, one more consensus (3.8%) was achieved in the second round. Finally, seven more statements (26.9%) reached a consensus in the simplified last round. Globally, a consensus was reached for almost half of the proposed statements (46.1%). CONCLUSIONS: The relatively low consensus rate obtained in this Delphi seems to confirm the discrepancy between Guideline recommendations and daily clinical practice. The data collected could represent the source for a possible guidelines' revision and the proposal of specific Good Practice Points in all those aspects with only little evidence available

Kinematics of Particles at Entrainment and Disentrainment

We address the issue of characterizing experimentally entrainment and disentrainment of sediment particles of cohesionless granular beds in turbulent open channel flows. Employing Particle Image Velocimetry, we identify episodes of entrainment and of disentrainment of bed particles by analysing the raw PIV images. We define a reference velocity for entrainment or disentrainment by space-averaging the flow field in the vicinity of the (entrained or disentrainned) particle and by time-averaging that space-average over a short duration encompassing the observed episode. All observations and measurements took place under generalized movement conditions and in non-controlled geometrical set-ups, resulting in unique databases of conditionally sampled turbulent flow kinematics associated with episodes of particle entrainment and of particle disentrainment. Exploring this database, the objective of this paper is to prove further insights on the dynamics of fluid-particle and particle-particle interactions at entrainment and disentrainment and to polemicize the use of a reference velocity to serve as a proxy for hydrodynamics actions responsible for entrainment or disentrainment. In particular, we quantify the reference velocity associated with entrainment and disentrainment episodes and discuss its potential to describe the observed motion vis-a-vis local bed micro-topography and the type of entrainment or disentrainment event. Entrainment may occur at a wide range of reference velocities, including smaller than mean (double-averaged) velocities. Anecdotal evidence was collected for some typologies of entrainment: (i) momentum transfer from flow to a single particle, (ii) momentum transfer from a perturbed local flow to a single particle, (iii) collective entrainment associated to momentum transfer between a moving and a resting particle and (iv) collective entrainment considered to be a dislodgment of several particles involving momentum transfer from other particles. In some of these cases, e.g., (ii) and (iii), the use of a reference velocity seems inadequate to characterize the entrainment episode. A word of caution about the use of entrainment models based on reference velocities is henceforth issued and contextualized. In the case of disentrainment, a reference velocity seems to constitute a better descriptor of the observed behaviour. The scatter in the observed values seems to express the contribution of bed micro-topography. All particles were found to experience frictional contacts with the resting bed surface particles, but some particles were stopped more abruptly due to the presence of an obstacle along their path. Most disentrainment of particles took place when the near-bed flow was featuring ejection events

Multidisciplinary management of anticoagulation therapy with International Normalized Ratio monitoring in general practice

No abstract availabl

Old apple (malus domestica L. Borkh) varieties with hypoallergenic properties: an integrated approach for studying apple allergenicity

Freshly consumed apples (Malus domestica L. Borkh) can cause allergic reactions because of the presence of four classes of allergens. Knowledge of the genetic factors affecting the allergenic potential of apples would provide important information for the selection of hypoallergenic genotypes, which can be combined with the adoption of new agronomical practices to produce fruits with a reduced amount of allergens. In the present research, a multiple analytical approach was adopted to characterize the allergenic potential of 24 apple varieties released at different ages (pre- and post-green revolution). A specific workflow was set up including protein quantification by means of polyclonal antibodies, immunological analyses with sera of allergic subjects, enzymatic assays, clinical assessments on allergic patients, and gene expression assays on fruit samples. Taken as a whole, the results indicate that most of the less allergenic genotypes were found among those deriving from selection processes carried out prior to the so-called "green revolution"

Alla ricerca della mela ipoallergenica: strategie e prospettive future

Lo studio delle allergie sta assumendo sempre maggiore importanza nell\u2019ambito della ricerca agroalimentare. La mela \ue8 uno dei frutti che \ue8 pi\uf9 frequentemente coinvolto in reazioni allergiche. Per questo motivo, numerosi progetti nazionali e internazionali (es. progetti europei SAFE e ISAFRUIT), sono stati dedicati allo studio delle allergie a questo frutto, chiarendone alcuni aspetti sia dal punto di vista clinico-allergologico, sia dal punto di vista biochimico-molecolare. Tali progetti hanno contribuito alla formazione di una prima rete di ricerca nazionale e internazionale su questo importante argomento. Nell\u2019ambito di queste iniziative, si colloca il progetto AGER-Melo che sta affrontando alcuni aspetti critici dell\u2019allergia alla mela, nell\u2019ottica del miglioramento della qualit\ue0 e della salubrit\ue0 del frutto, coinvolgendo competenze mediche, biochimiche, genetico-molecolari e agro-biotecnologiche. Nel melo sono state identificate 4 famiglie di principali allergeni: Mal d 1, 2, 3 e 4, ognuna composta da numerose isoforme che aumentano la complessit\ue0 delle ricerche. Nell\u2019AGER-Melo sono state previste diverse linee di ricerca allo scopo di valutare il potenziale allergenico della mela e i fattori endogeni ed esogeni che lo determinano. Tali valutazioni sono attualmente in corso mediante l\u2019impiego di diverse tecniche quali l\u2019utilizzo di sieri di pazienti allergici, di anticorpi policlonali specifici per ogni famiglia di allergeni, di analisi della espressione di ogni singolo allergene e dell\u2019intero trascrittoma dei genotipi pi\uf9 promettenti. L\u2019identificazione dei determinanti dell\u2019allergenicit\ue0 della mela permetter\ue0 di ottenere variet\ue0 ipoallergeniche anche mediante la progettazione di programmi di miglioramento genetico dedicati a questo scopo con l\u2019obiettivo finale di consentire anche a persone allergiche di consumare questo frutto particolarmente noto anche per il suo valore nutrizionale. Questo studio \ue8 finanziato dal Progetto AGER-Melo, grant n\ub0 2010-2119

Properties of aluminosilicate refractories with synthesized boron-modified TiO2 nanocrystals

An efficient microwave supported synthesis, with a reaction time of only one and a half minute, to prepare boron-modified titania nanocrystals TiO2:(B), was developed. The nanocrystals were obtained by hydrolysis of titanium tetraisopropoxide (TTIP) together with benzyl alcohol and boric acid, and the approach did not need surfactants use and a final calcination step. The produced TiO2:(B) nanocrystals were characterized in detail by low magnification Transmission Electron Microscopy (TEM), Inductively Coupled Plasma-Atomic Emission Spectroscopy (ICP-AES), X-Ray Diffractometry (XRD), and a Micro Raman Spectroscopy. One of the obtained samples was then tested as an additive in various amounts in a typical aluminosilicate refractory composition. The effects of these additions in bricks were evaluated, according to UNI EN 196/2005, in terms of thermo-physical and mechanical properties: diffusivity, bulk density, apparent density, open and apparent porosity and cold crushing strength. Bricks’ microstructure was analysed by Scanning Electron Microscopy (SEM) and energy dispersion spectroscopy (EDS). The bricks obtained with nanoadditives presented improved mechanical characteristics with respect to the typical aluminosilicates, presumably because of a better compaction during the raw materials’ mixing stage