Cloning and prokaryotic expression of the globular head domain of hemagglutinin antigen (HA1) of influenza A (H3N2) virus in Escherichia coli and Bacillus subtilis

Background: The influenza virus hemagglutinin is the major surface protein of the influenza A virus which is composed of HA1 and HA2 subunits. HA1 has an important role in binding of virus to cells and designing neutralizing antibodies. Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis) both are known as the most useful prokaryotic hosts to express recombinant proteins. The aim of this study was to clone and express recombinant HA1protein in E. coli and B. subtilis bacteria.Materials and Methods: HA1 gene was cloned into pET-28a vector and pHT43 shuttle vector and then, both transformed to E. coli. The recombinant plasmids were extracted and then transformed into the BL21 and WB600 as expressing hosts. After induction with isopropyl-β-d-thiogalactoside (IPTG), expressed recombinant protein was analyzed by SDS-PAGE. Finally, the expressed protein was confirmed by the Western blot.Results: HA1 gene was cloned into pET-28a vector and pHT43 shuttle vector and then, both transformed to E. coli.The recombinant plasmids were extracted and then transformed into the BL21 and WB600 as expressing hosts. After induction with isopropyl-β-d-thiogalactoside (IPTG), expressed recombinant protein was analyzed by SDS-PAGE. Finally, the expressed protein was confirmed by the Western blot.Conclusion: This study demonstrated a strategy for production and purification of recombinant protein in large scale to test as vaccine candidate against influenza and it’s potentially immunogenicity be assessed in animal models

Molekularna karakterizacija i otpornost na antibiotike bakterije Clostridium perfringens izolirane iz trupova goveda i ovaca u klaonicama Shiraza u južnom Iranu

Clostridium perfringens type A food-borne poisoning is often caused by C. perfringens enterotoxin (CPE) encoded by chromosomal cpe. Contamination of meat with C. perfringens usually leads to food poisoning outbreaks. To find more information regarding the causative agent, we focused on the identification of type A containing cpe and netB genes in cattle and sheep carcasses slaughtered at Shiraz slaughterhouse and investigated the prevalence of antibiotic-resistant plasmid in isolated C. perfringens. 200 specimens were randomly collected by swabbing the whole outer and inner surface of the carcasses, and processed for selective culture on sulfadiazine polymyxin sulphate agar (SPS). The suspected colonies were further identified using species-specific primers as to confirm the presence of the cpa, cpe, netB and tetracycline and enrofloxacin gene resistance patterns. Our results demonstrated that out of 90 and 70 colonies of the positive cultures from cattle and sheep samples, respectively, 40% and 35.7% of the suspected colonies were identified as C. perfringens type A by PCR assay. Moreover, from those type A isolates, only 1 (2.7%) isolate was positive for both cpe and netB genes in the cattle carcasses. The MIC values also showed high tetracycline resistance patterns for cattle (45.8%) and sheep (92.3%) while all of the PCR positive C. perfringens type A isolates were susceptible to enrofloxacin. The high prevalence of C. perfringens in slaughtered animals with a high rate of resistance to tetracycline implies the need for caution in the use of antibiotic in food animals.Trovanje hranom uzrokovano bakterijom Clostridium perfringens tipa A često uzrokuje C. perfringens enterotoksin (CPE), kodiran kromosomskim cpe. Kontaminacija mesa s C. perfringens obično uzrokuje otrovanje hranom. Da bismo doznali više informacija o uzročniku, iz trupova goveda i ovaca zaklanih u klaonicama Shiraza, identificirali smo tip A koji sadržava gene cpe i netB. Osim toga, u izoliranim bakterijama C. perfringens utvrdili smo prevalenciju plazmida rezistentnih na antibiotike. Obriskom cijele vanjske i unutarnje površine nasumično odabranih trupova, prikupljeno je 200 uzoraka koji su obrađeni selektivnom kulturom na sulfadiazin-polimiksin sulfatnom agaru (SPS). Sumnjive kolonije dodatno su identificirane primjenom specifičnih početnica kako bi se potvrdila prisutnost gena cpa, cpe, netB te gena za otpornost na tetraciklin i enrofloksacin. Naši su rezultati pokazali da je PCR analizom od 90, odnosno 70 kolonija pozitivnih kultura iz uzoraka goveda i ovaca, njih 40 %, odnosno 35,7 % identificirano kao C. perfringens tipa A. Štoviše, iz izolata tipa A dobivenih od goveđih trupova, samo je jedan izolat (2,7 %) bio pozitivan i za gene cpe i za netB. MIC vrijednosti također su pokazale visoku razinu otpornosti na tetracikline kod goveda (45,8 %) i ovaca (92,3 %), dok su svi PCR pozitivni na C. perfringens tipa A bili osjetljivi na enrofloksacin. Visoka prevalencija bakterije C. perfringens kod zaklanih životinja zajedno s visokom stopom otpornosti na tetraciklin upućuje na potrebu za oprezom u primjeni antibiotika kod životinja koje služe za ljudsku hranu

One-Hundred-Year Efforts by the Pasteur Institute of Iran as a Part of the Rabies Infectious Control Puzzle in the World

Rabies is a fatal zoonotic disease that occurs usually via animal bites. Rabies is currently an economic health problem in Iran and almost all of the provinces are more or less involved in this. For nearly a century, many researchers have tried to control rabies in Iran. To date, Iran has played a major role in the new scientific advances in the rabies treatment field. The Pasteur Institute of Iran was established in a 2.2 ha land to provide public health services for the prevention and treatment of infectious diseases. Since then, the official activity of the Pasteur Institute of Iran has begun. The operations of the Institute are still being developed quantitatively and qualitatively. The aim of this study is to introduce One-Hundred-Year Efforts by the Pasteur Institute of Iran as a Part of the Rabies Infectious Control Puzzle in the World

Insights into the SARS-CoV2 Outbreak; the Great Global Challenge: A Mini Review

The emergence of COVID-19 outbreak caused by a novel coronavirus, was reported in Wuhan, Hubei, China in December 2019, and it spread rapidly across the world, resulting in the World Health Organization announcing a global health emergency on 30 January 2020. The first death caused by it in Iran was reported in February 2020 and spread rapidly across the country. The novel virus responsible for sever complications even death in all age groups, especially in older adults and people of any age who have serious underlying medical conditions. Researchers around the world are studying to identify the novel virus, SARS-CoV2, with the aim of preventing its dissemination and to reduce its complications. Here, we discuss the available information regarding the SARS-CoV2 Outbreak and disease caused as a mini-review

Confrontations in the historical part of Shahnameh with the approach of Louis Strauss

 The opposition of the opposites is also reflected in the myth of the nations and one of the main fields of the myth is the struggle of different forces. Myth is the mother of epic, and any nation that does not have a myth is also useless of epic, and the main background of the epic of any nation is its myth. So this fundamental concept has also found its way into the epic; But in the process of materialization of phenomena from myth to epic, mythical concepts are formed differently in the epic, and during this process, the confrontation of human beings or heroes with evil forces, the confrontation of two tribes and clans, The two heroes become one with each other. In general, it can be said that epic is the fruit of man's struggle with anything that is against his dreams and aspirations and in opposition to his will and action. Meanwhile, the contrast between the phenomena of nature such as night and day, darkness and light, drought and wet season, greenery and autumn and the like, had caused the human way of thinking and worldview based on the same contrast. And as a result, human mythology is full of these contradictions. Moreover, even in later periods, such a way of thinking has prevailed in the human mind and historical data have been analyzed and explained based on this approach. Another important factor in the existence of confrontation and contradiction in mythology is the existence of secondary ideas in these works; Because the important principle in the dual thought of the myths of ancient Iran is the conflict between the demonic forces and the Ahura forces, which considers the whole world as the place of these two opposing forces; Influenced by this idea, we always encounter confrontation and war between groups in Shahnameh. One of the important sources and a collection of myths and legends and even valuable historical contents of this region is Ferdowsi's Shahnameh, which is divided into three parts: mythology (from the time of Kiomars to the kingdom of Fereydoun), heroism (from the rise of the blacksmith to The assassination of Rostam and the reign of Bahman, son of Esfandiar) and historical (from the reign of Bahman and the birth of Alexander to the opening of Iran to the Arabs) has been divided

Confrontations in the historical part of Shahnameh with the approach of Louis Strauss

 The opposition of the opposites is also reflected in the myth of the nations and one of the main fields of the myth is the struggle of different forces. Myth is the mother of epic, and any nation that does not have a myth is also useless of epic, and the main background of the epic of any nation is its myth. So this fundamental concept has also found its way into the epic; But in the process of materialization of phenomena from myth to epic, mythical concepts are formed differently in the epic, and during this process, the confrontation of human beings or heroes with evil forces, the confrontation of two tribes and clans, The two heroes become one with each other. In general, it can be said that epic is the fruit of man's struggle with anything that is against his dreams and aspirations and in opposition to his will and action. Meanwhile, the contrast between the phenomena of nature such as night and day, darkness and light, drought and wet season, greenery and autumn and the like, had caused the human way of thinking and worldview based on the same contrast. And as a result, human mythology is full of these contradictions. Moreover, even in later periods, such a way of thinking has prevailed in the human mind and historical data have been analyzed and explained based on this approach. Another important factor in the existence of confrontation and contradiction in mythology is the existence of secondary ideas in these works; Because the important principle in the dual thought of the myths of ancient Iran is the conflict between the demonic forces and the Ahura forces, which considers the whole world as the place of these two opposing forces; Influenced by this idea, we always encounter confrontation and war between groups in Shahnameh. One of the important sources and a collection of myths and legends and even valuable historical contents of this region is Ferdowsi's Shahnameh, which is divided into three parts: mythology (from the time of Kiomars to the kingdom of Fereydoun), heroism (from the rise of the blacksmith to The assassination of Rostam and the reign of Bahman, son of Esfandiar) and historical (from the reign of Bahman and the birth of Alexander to the opening of Iran to the Arabs) has been divided

Confrontations in the historical part of Shahnameh with the approach of Louis Strauss

 The opposition of the opposites is also reflected in the myth of the nations and one of the main fields of the myth is the struggle of different forces. Myth is the mother of epic, and any nation that does not have a myth is also useless of epic, and the main background of the epic of any nation is its myth. So this fundamental concept has also found its way into the epic; But in the process of materialization of phenomena from myth to epic, mythical concepts are formed differently in the epic, and during this process, the confrontation of human beings or heroes with evil forces, the confrontation of two tribes and clans, The two heroes become one with each other. In general, it can be said that epic is the fruit of man's struggle with anything that is against his dreams and aspirations and in opposition to his will and action. Meanwhile, the contrast between the phenomena of nature such as night and day, darkness and light, drought and wet season, greenery and autumn and the like, had caused the human way of thinking and worldview based on the same contrast. And as a result, human mythology is full of these contradictions. Moreover, even in later periods, such a way of thinking has prevailed in the human mind and historical data have been analyzed and explained based on this approach. Another important factor in the existence of confrontation and contradiction in mythology is the existence of secondary ideas in these works; Because the important principle in the dual thought of the myths of ancient Iran is the conflict between the demonic forces and the Ahura forces, which considers the whole world as the place of these two opposing forces; Influenced by this idea, we always encounter confrontation and war between groups in Shahnameh. One of the important sources and a collection of myths and legends and even valuable historical contents of this region is Ferdowsi's Shahnameh, which is divided into three parts: mythology (from the time of Kiomars to the kingdom of Fereydoun), heroism (from the rise of the blacksmith to The assassination of Rostam and the reign of Bahman, son of Esfandiar) and historical (from the reign of Bahman and the birth of Alexander to the opening of Iran to the Arabs) has been divided

Confrontations in the historical part of Shahnameh with the approach of Louis Strauss

 The opposition of the opposites is also reflected in the myth of the nations and one of the main fields of the myth is the struggle of different forces. Myth is the mother of epic, and any nation that does not have a myth is also useless of epic, and the main background of the epic of any nation is its myth. So this fundamental concept has also found its way into the epic; But in the process of materialization of phenomena from myth to epic, mythical concepts are formed differently in the epic, and during this process, the confrontation of human beings or heroes with evil forces, the confrontation of two tribes and clans, The two heroes become one with each other. In general, it can be said that epic is the fruit of man's struggle with anything that is against his dreams and aspirations and in opposition to his will and action. Meanwhile, the contrast between the phenomena of nature such as night and day, darkness and light, drought and wet season, greenery and autumn and the like, had caused the human way of thinking and worldview based on the same contrast. And as a result, human mythology is full of these contradictions. Moreover, even in later periods, such a way of thinking has prevailed in the human mind and historical data have been analyzed and explained based on this approach. Another important factor in the existence of confrontation and contradiction in mythology is the existence of secondary ideas in these works; Because the important principle in the dual thought of the myths of ancient Iran is the conflict between the demonic forces and the Ahura forces, which considers the whole world as the place of these two opposing forces; Influenced by this idea, we always encounter confrontation and war between groups in Shahnameh. One of the important sources and a collection of myths and legends and even valuable historical contents of this region is Ferdowsi's Shahnameh, which is divided into three parts: mythology (from the time of Kiomars to the kingdom of Fereydoun), heroism (from the rise of the blacksmith to The assassination of Rostam and the reign of Bahman, son of Esfandiar) and historical (from the reign of Bahman and the birth of Alexander to the opening of Iran to the Arabs) has been divided

Confrontations in the historical part of Shahnameh with the approach of Louis Strauss

 The opposition of the opposites is also reflected in the myth of the nations and one of the main fields of the myth is the struggle of different forces. Myth is the mother of epic, and any nation that does not have a myth is also useless of epic, and the main background of the epic of any nation is its myth. So this fundamental concept has also found its way into the epic; But in the process of materialization of phenomena from myth to epic, mythical concepts are formed differently in the epic, and during this process, the confrontation of human beings or heroes with evil forces, the confrontation of two tribes and clans, The two heroes become one with each other. In general, it can be said that epic is the fruit of man's struggle with anything that is against his dreams and aspirations and in opposition to his will and action. Meanwhile, the contrast between the phenomena of nature such as night and day, darkness and light, drought and wet season, greenery and autumn and the like, had caused the human way of thinking and worldview based on the same contrast. And as a result, human mythology is full of these contradictions. Moreover, even in later periods, such a way of thinking has prevailed in the human mind and historical data have been analyzed and explained based on this approach. Another important factor in the existence of confrontation and contradiction in mythology is the existence of secondary ideas in these works; Because the important principle in the dual thought of the myths of ancient Iran is the conflict between the demonic forces and the Ahura forces, which considers the whole world as the place of these two opposing forces; Influenced by this idea, we always encounter confrontation and war between groups in Shahnameh. One of the important sources and a collection of myths and legends and even valuable historical contents of this region is Ferdowsi's Shahnameh, which is divided into three parts: mythology (from the time of Kiomars to the kingdom of Fereydoun), heroism (from the rise of the blacksmith to The assassination of Rostam and the reign of Bahman, son of Esfandiar) and historical (from the reign of Bahman and the birth of Alexander to the opening of Iran to the Arabs) has been divided