73 research outputs found

    Enquête sur l’Importance des Ressources Végétales pour la Population Riveraine du Parc National de la Langue de Barbarie (Sénégal)

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    Les espèces vĂ©gĂ©tales constituent une composante essentielle de la biodiversitĂ©. Ainsi au SĂ©nĂ©gal plusieurs Ă©tudes ont Ă©tĂ© effectuĂ©es sur ces espèces. Cependant celles qui portent Ă  la fois sur les ligneux et les herbacĂ©es sont rares. Ce travail a Ă©tĂ© rĂ©alisĂ© entre 2012 et 2014 pour Ă©valuer l’importance des ressources vĂ©gĂ©tales pour la population riveraine du Parc National de la Langue de Barbarie (PNLB). Une enquĂŞte ethnobotanique basĂ©e sur un questionnaire a Ă©tĂ© rĂ©alisĂ©e. La valeur d’usage, le facteur de consensus et le niveau de fidĂ©litĂ© ont Ă©tĂ© Ă©valuĂ©s. L’analyse des rĂ©sultats montre que Prosopis glandulosa (1,05) prĂ©sente la plus grande valeur d’usage suivie d’Acacia tortilis subsp raddiana (0,83). Les usages les plus courants sont l’alimentation humaine (19,90 %), l’usage mĂ©dicinal (19,40 %) et l’alimentation animale (16,50 %). Les facteurs de consensus sont plus Ă©levĂ©s pour l’alimentation humaine et l’usage mĂ©dicinal. La flore mĂ©dicinale utilisĂ©e par les riverains du PNLB est plus diversifiĂ©e avec 67 espèces rĂ©parties dans 32 familles et 56 genres. Elle est suivie de l’alimentation humaine avec 52 espèces rĂ©parties dans 32 familles et 45genres. Les rĂ©sultats sur le niveau de fidĂ©litĂ© des espèces aux catĂ©gories d’usages les plus citĂ©es montrent l’existence d’espèces Ă  usages exclusifs avec 100% de niveaux de fidĂ©litĂ© et d’espèces Ă  usages multiples avec moins de 100% de niveau de fidĂ©litĂ©. Le niveau de fidĂ©litĂ© des espèces utilisĂ©es dans l’alimentation humaine, animale ainsi que dans la mĂ©decine traditionnelle et leur valeur d’usage sont corrĂ©lĂ©s nĂ©gativement. Les ressources vĂ©gĂ©tales sont importantes pour la population riveraine du Parc National de la Langue de Barbarie qui les utilise dans plusieurs catĂ©gories d’usages. Cependant une Ă©tude plus approfondie sur les espèces mĂ©dicinales serait bĂ©nĂ©fique pour l’humanitĂ©.   Plant species are an essential component of biodiversity. Thus in Senegal several studies have been carried out on these species. However, those relating to both ligneous and herbaceous plants are rare. This work was carried out between 2012 and 2014 to assess the importance of plant resources for the local population of the Thongue of Barbarism National Park (TBNP). An ethnobotanical survey based on a questionnaire was carried out. Analysis of the results shows that  Prosopis glandulosa (1.05) shows the highest use value followed by Acacia tortilis subsp raddiana (0.83). The most common use are food (19.90%), medicinal use (19.40%) and animal feed (16.50%). Consensus factor are highest for human food and medicinal use. The medicinal flora used by residents of the NPLB is more diversified with 67 species distributed in 32 families and 56 genera. The families most represented in this medicinal flora are the Fabaceae (22.38%), the Malvaceae (8.95%) and the Combretaceae (5.97%). It is followed by human food with 52 species divided into 32 families and 45 genera. The results on the level of fidelity of species to the most cited use categories show the existence of species for exclusive use with 100% fidelity levels and multiple use species with less than 100% fidelity level. The level of fidelity of species used in human and animal food as well as in traditional medicine and their use value are negatively correlated. Plant resources are important for the local population of the Thongue of Barbarism National Park who use them in several categories of uses. The importance of  plant resources for the population is well known. However, further study of medicinal species would be beneficial to mankind

    The Drying Kinetics Study of Couscous for its Conservation,Comsumption and Urban Marketing

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    The combined effects of the devaluation of the CFA franc in 1992, the food crisis of 2008 and the strong demand for grain products developed urban dwellers have proven to be a necessity of development of grain products manufacturing units marketable and storable without any risk of alteration for cereal craftsmen. This contribution conducted at 40 ° C and 55 ° C with air speeds of 1m / s 2m / s and 3m / s is in the kinetic study of a technological process couscous drying to determine the optimum conditions of the process of dehydration. She found that the drying kinetics of couscous takes place in three phases; indicating that couscous is rich in water and that it can be stored at room temperature unless it is dried. Henceforth it is imperative to have a good knowledge and mastery of these three phases that impact on product quality This study showed that the experiments performed at a speed of air 3m / s at 40 ° C temperatures and 55 ° C, show a brown coloration due to Maillard reactions. We see that the browning phenomena are more pronounced at the speed of 3m / s and 55 ° C. It is found that the slow drying is better suited to the quick dry couscous. In addition to further drying the air velocity of 1m / s correlated with the temperature of 40 ° C is the most suitable as well as good monitoring of the evolution of the temperature during the drying phase warm up for a good product.

    Reemergence of Sylvatic Dengue Virus in Southern Senegal, 2021

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    As part of the syndromic surveillance of fever in Senegal, the virology department at Institut Pasteur de Dakar (IPD) in collaboration with the Epidemiology Unit and the Senegalese Ministry of Health conducted syndromic surveillance of fever in Senegal. Sample are from all suspected arboviral infections patients attending any of the sentinel sites. Collected blood samples were sent on a weekly basis at WHOCC for arboviruses and hemorrhagic fever viruses for screening of seven medically important arboviruses, including dengue virus (DENV). From January to December 2021, 2010 suspected cases were received among them 124 for confirmed to be DENV+ by RT-qPCR attempt of serotyping led to the detection of atypical DENV case from Sare Yoba area (Kolda region) which is unable to be correctly assigned to a serotype by the available tools (TIB Molbiol Modular Dx Dengue typing kit). Performed genome sequencing et phylogenetic analysis leads to the identification of a sylvatic DENV-2 strain closely related to a virus previously detected in Guinee-Bissau in 2009. This finding constitutes proof of the contemporary circulation of DENV-2 strain belonging to the sylvatic cycle in addition to well-known epidemic strains; this adds a piece of complexity to dengue management in Senegal. Alarmingly, it calls for improved genomic surveillance of DENV to know the genetic diversity of circulating strains in order to strengthen future vaccination policies

    Étude in vitro de l’effet antifalcémiant des globules rouges et de l’activité antioxydante d’extraits de la poudre de racines de Maytenus senegalensis Lam (Celestraceae)

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    La drépanocytose est une hémoglobinopathie largement répandue chez les populations africaines noires. Actuellement, très peu de traitements conventionnels existent sur le marché, d’où le recours à la médecine traditionnelle. Maytenus senegalensis est une plante prescrite par les tradipraticiens dans la prise en charge de la drépanocytose. L’activité antifalcémiante des extraits de la poudre de racines de Maytenus senegalensis a été étudiée dans ce travail. Après obtention de l’extrait hexanique par extraction à l’aide d’un Soxhlet, le marc est macéré dans de l’acétate d’éthyle puis dans du méthanol pour donner les extraits correspondants. L’activité des fractions a été évaluée sur des drépanocytes de type SS. Les activités antifalcémiantes les plus importantes ont été observées pour les fractions polaires du méthanol et de l’acétate d’éthyle avec des taux d’inversion de la falciformation respectivement de 77% et de 65% à 10 mg/mL. Les activités antioxydantes de M. senegalensis (CI50 = 0,195 ± 0,004 mg/mL) et de l’acide ascorbique (CI50 = 0,078 ± 0,002 mg/mL) ont été déterminées. Les pouvoirs antioxydants (PA) montrent que l’activité antiradicalaire de l’acide ascorbique (PA = 12,85) est 2,5 fois meilleure que celle de M. senegalensis (PA = 5,14). Les résultats de l’étude justifient l’utilisation traditionnelle des racines de Maytenus senegalensis dans la prise en charge de la drépanocytose.Mots clés : Hémoglobine, drépanocytose, Maytenus senegalensis, activité antifalcémiante, stress oxydant, radicaux libres, activité antioxydante

    L’éléphantiasis vulvo-clitoridien: à propos d’un nouveau cas

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    L'éléphantiasis vulvo-clitoridien d'origine filarienne est une affection très rare. Nous rapportons un nouveau cas chez une femme de 33 ans suivie dans un service de Maladies Infectieuses pour filariose lymphatique. Elle avait une masse vulvo-clitoridienne qui évoluait depuis plus de 10 ans. Une résection clitoridienne et une plastie vulvaire a été réalisée. Les résultats fonctionnels et esthétiques étaient satisfaisants.Key words: Eléphantiasis, filariose, vulvo-clitoridie

    SARS-CoV-2 seroprevalence in pregnant women during the first three COVID-19 waves in The Gambia

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    OBJECTIVES: SARS-CoV-2 transmission in Sub-Saharan Africa has probably been underestimated. Population-based seroprevalence studies are needed to determine the extent of transmission in the continent. METHODS: Blood samples from a cohort of Gambian pregnant women were tested for SARS-CoV-2 total receptor binding domain (RBD) IgM/IgG before (Pre-pandemic: October-December 2019), and during the pandemic (Pre-wave1: February-June 2020; Post-wave1: October-December 2020, Post-wave2: May-June 2021; and Post-wave3: October-December 2021). Samples reactive for SARS-CoV-2 total RBD IgM/IgG were tested in specific S1- and nucleocapsid (NCP) IgG assays. RESULTS: SARS-CoV-2 total RBD IgM/IgG seroprevalence was 0.9% 95%CI (0.2, 4.9) in Pre-pandemic; 4.1% (1.4, 11.4) in Pre-wave1; 31.1% (25.2, 37.7) in Post-wave1; 62.5% (55.8, 68.8) in Post-wave2 and 90.0% (85.1, 93.5) in Post-wave3. S-protein IgG and NCP-protein IgG seroprevalence also increased at each Post-wave period. Although S-protein IgG and NCP-protein IgG seroprevalence was similar at Post-wave1, S-protein IgG seroprevalence was higher at Post-wave2 and Post-wave3, [prevalence difference (PD) 13.5 (0.1, 26.8) and prevalence ratio (PR) 1.5 (1.0, 2.3) in Post-wave2; and 22.9 (9.2, 36.6) and 1.4 (1.1, 1.8) in Post-wave3 respectively, p<0.001]. CONCLUSION: SARS-CoV-2 transmission in The Gambia during the first three COVID-19 waves was high, differing significantly from official numbers of COVID-19 cases reported. Our findings are important for policy makers in managing the near-endemic COVID-19

    Long-term cellular immunity of vaccines for Zaire Ebola Virus Diseases

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    Recent Ebola outbreaks underscore the importance of continuous prevention and disease control efforts. Authorized vaccines include Merck’s Ervebo (rVSV-ZEBOV) and Johnson & Johnson’s two-dose combination (Ad26.ZEBOV/MVA-BN-Filo). Here, in a five-year follow-up of the PREVAC randomized trial (NCT02876328), we report the results of the immunology ancillary study of the trial. The primary endpoint is to evaluate long-term memory T-cell responses induced by three vaccine regimens: Ad26–MVA, rVSV, and rVSV–booster. Polyfunctional EBOV-specific CD4+ T-cell responses increase after Ad26 priming and are further boosted by MVA, whereas minimal responses are observed in the rVSV groups, declining after one year. In-vitro expansion for eight days show sustained EBOV-specific T-cell responses for up to 60 months post-prime vaccination with both Ad26-MVA and rVSV, with no decline. Cytokine production analysis identify shared biomarkers between the Ad26-MVA and rVSV groups. In secondary endpoint, we observed an elevation of pro-inflammatory cytokines at Day 7 in the rVSV group. Finally, we establish a correlation between EBOV-specific T-cell responses and anti-EBOV IgG responses. Our findings can guide booster vaccination recommendations and help identify populations likely to benefit from revaccination
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