THE EFFECT OF ADDING SECTION AND LEVEL OF DIFFERENT BANANA HEART ON THE ORGANOLEPTIC QUALITIES OF BEEF SAUSAGE

This study aims to determine the effect of adding parts of different level banana heart and the interaction between parts of different level banana to the organoleptic quality of beef sausages. The study design was completely randomized factorial 3 x 3. Factors A banana is a part where the A1 (the inside of the banana white), A2 (the outside of the heart of the banana light purple), and A3 (a mixture of the inner and outer parts banana heart). Factor B is the level where the addition of banana B1 (0%), B2 (15%), and B3 (30%). Parameters measured were the level of resilience, color, aroma, taste and preference. The research result of addition of a section banana heart at different levels indicate that there are highly significant effect on the elasticity, color, aroma, taste and preferences of beef sausage with the average value of 2,804, which means a bit chewy, 2.767 indicates that the sausages brown, 2.433 which shows sausage savoury aroma, which shows the value 2,352 sausages taste bad, 2, 815 which shows the value of rather like. Based on the results of this study concluded that the addition of banana heart mixture of the inner and outer generate resilience, aroma, and taste of the sausages were better than those just added to the inside or the outside only. Additions to the level of 30% better than the addition of 15% and without the addition (0%). Interaction factor and level parts of banana heart showed a different response at the level of resilience, color, aroma, taste and preference of beef sausag

Pengaruh Waktu Thawing Terhadap Kualitas Spermatozoa Ayam Buras

The purpose of this study was to determine the effect of thawing time on the quality of native chicken spermatozoa. The research design used in this study was a completely randomized design (CRD) with 5 treatment levels and 5 replications. The treatments used were P1 = thawing time of 20 seconds, P2 = thawing time of 30 seconds, P3 = thawing time of 40 seconds, P4 = thawing time of 50 seconds, P5 = thawing time of 60 seconds. Semen that has been diluted to be packed in 5 ministraw 0.25 ml, then equilibrated for 2 hours. After equilibration, proceed with the freezing process (prefreezing). Then the straws were stored in a liquid N2 container (-196°C) for 24 hours. frozen semen was thawed again with warm water at 37°C according to the treatment. Parameters observed were sperm motility and recovery rate. The results of this study showed that thawing time had no significant effect (P>0.05) on sperm motility and recovery rate. It was concluded that the thawing time of 20 to 60 seconds could be used in freezing native chicken semen.The purpose of this study was to determine the effect of thawing time on the quality of native chicken spermatozoa. The research design used in this study was a completely randomized design (CRD) with 5 treatment levels and 5 replications. The treatments used were P1 = thawing time of 20 seconds, P2 = thawing time of 30 seconds, P3 = thawing time of 40 seconds, P4 = thawing time of 50 seconds, P5 = thawing time of 60 seconds. Semen that has been diluted to be packed in 5 ministraw 0.25 ml, then equilibrated for 2 hours. After equilibration, proceed with the freezing process (pre-freezing). Then the straws were stored in a liquid N2 container (-196°C) for 24 hours. frozen semen was thawed again with warm water at 37°C according to the treatment. Parameters observed were sperm motility and recovery rate. The results of this study showed that thawing time had no significant effect (P>0.05) on sperm motility and recovery rate. It was concluded that the thawing time of 20 to 60 seconds can be applied to frozen native chicken semen. The purpose of this study was to determine the effect of thawing time on the quality of native chicken spermatozoa. The research design used in this study was a completely randomized design (CRD) with 5 treatment levels and 5 replications. The treatments used were P1 = thawing time of 20 seconds, P2 = thawing time of 30 seconds, P3 = thawing time of 40 seconds, P4 = thawing time of 50 seconds, P5 = thawing time of 60 seconds. Semen that has been diluted to be packed in 5 ministraw 0.25 ml, then equilibrated for 2 hours. After equilibration, proceed with the freezing process (pre-freezing). Then the straws were stored in a liquid N2 container (-196°C) for 24 hours. frozen semen was thawed again with warm water at 37°C according to the treatment. Parameters observed were sperm motility and recovery rate. The results of this study showed that thawing time had no significant effect (P>0.05) on sperm motility and recovery rate. It was concluded that the thawing time of 20 to 60 seconds can be applied to frozen native chicken semen

Physiological Response of Bali Cattle to Feeding Substitution of Balinese Fermentation Straw

This study aims to see the physiological response of Bali cattle to the feeding of fermented straw substitution. A total of 12 head of Bali cattle average age five years. This research was conducted from August to September 2017 in Cenrana Village, Kahu Sub-district, Bone District, which is a location in the business of Berdikari Berdikari Mallomo Utama. This study used a randomized block design (RBD) with 4 treatments and 3 replications so that there were 12 experimental units, the procedure was P0: 100% elephant grass + 100% fermentation control, P1: elephant grass 75% + generation fermentation 25%, P2: 50%, elephant grass + 50% fermenting straw, and P3: Elephant grass 25% + 75% fermentation straw. The parameters observed were body temperature, respiration rate, and liver rate. The data obtained were analyzed variance and continued with LRD test. The results showed that the substitution of hay fermentation had a significant effect (P .01) on the physiological response of Balinese cow heart, but no significant effect (P .05) to the body temperature and the rate of respiration of Bali cattle. The conclusion that by giving fermented straw substitution feed provide an effect on heart rate (58.33 / min) with treatment 25% elephant grass + 75% fermented straw in the normal range for Bali cattle

Performance of Bali Cattle on Substitution Feeding Fermented Straw

The purpose of this research is to know the increase of body weight, feed consumption, feed efficiency and conversion of Bali cattle feed given fermented straw as substitution of Elephant Grass. The method used in this Experimental study Method with Randomized Block Design with four treatments and three replications. P2: Elephant Grass 100% (control) P1: Elephant Grass 75% + 25% fermented straw, P2 treatment: Elephant Grass 50% + 50% fermentation straw and P3 Treatment: 25% Grass + Finger straw 75 %. The observed changes were weight gain (kg/day), feed consumption (kg/day), feed efficiency and feed conversion in Bali cattle. The data obtained were analyzed in various ways with Duncan. The result of this research showed that by giving substitution of fermented straw to determine the performance of bovine cow have a real effect (P ≤.05) to the value of body weight gain, feed consumption, feed conversion and efficiency of feed. It shows that with the difference of feeding substitution of fermented straw can give the real effect on the increase of body weight of Bali cattle with feeding of forage grass 50% elephant + 50% fermented straw

PENGARUH PENAMBAHAN BAGIAN DAN LEVEL JANTUNG PISANG TERHADAP KUALITAS FISIK SOSIS DAGING SAPI

ABSTRACT. This study aims to determine the effect and level section of banana and interaction both on the physical quality of beef sausage. Research using a completely randomized design factorial pattern 3 x 3 each with three replications. Factor is 3 parts of banana (Interior, Exterior and Mixed), Factor B is the level of use (0%, 15 and 30%). Parameters measured were cooking shrinkage, breaking power, power iris and pH. The study concluded that: 1) Addition of banana intact produce sausages that have tenderness (power break) and iris power higher than the sausage with the addition of banana inside and the outside, but did not make a difference in the shrinkage cookware and pH sausage. 2) The higher levels of addition of the higher shrinkage cookware, tenderness and a social and iris power, no difference in the effect of banana on the pH level of the sausage. 3) The interaction factor and level section of banana showed no difference in response to shrinkage cookware, powerbreaking, power and pH sausage slices.

PENINGKATAN KETERAMPILAN BUDIDAYA TERNAK UNGGAS BAGI PETERNAK AYAM LOKAL DI KABUPATEN KOLAKA MELALUI BIMBINGAN TEKNIS INSEMINASI BUATAN DAN METODE PERSILANGAN

Ayam lokal merupakan jenis ayam bukan ras (buras) Indonesia yang keberadaannya masih belum sepenuhnya mendapatkan perhatian dari usaha pembibitan sehingga produktivitasnya masih tergolong rendah. Peningkatan produktivitas ayam lokaldapat  dilakukan dengan program persilangan pada pemeliharaan sistem intensif. Sistem perkawinan buatan (inseminasi buatan) pada pemeliharaan sistem intensif cukup efektif dilakukan karena cukup efisien dalam penggunaan jumlah pejantan. Peternak ayam lokal di Kabupaten Kolaka belum menerapkan metode inseminasi buatan dan upaya persilangan pada ternak unggas mereka. Hal ini mendorong penulis melakukan kegiatan pengabdian kepada masyarakat yang bertujuan untuk meningkatkan pengetahuan dan keterampilan bagi peternak ayam lokal di Kecamatan Watubangga Kabupaten Kolaka terkait penerapan teknologi inseminasi buatan (IB) pada unggas. Rangkaian kegiatan yang meliputi: 1) Membuat Rencana Kegiatan Bimtek IB pada Unggas, 2) Membuat panduan tentang inseminasi buatan (IB) pada unggas, 3) Merancang alat IB unggas, 4) Mendemonstrasikan teknik IB ayam, 5) Evaluasi hasil praktek IB ayam. Bimbingan teknis dilakukan terhadap 8 peternak ayam lokal di Kecamatan Watubangga Kabupaten Kolaka. Terjadi peningkatan pengetahuan masyarakat mengenai inseminasi buatan pada unggas setelah dilakukan bimbingan teknis yang ditandai dengan peningkatan nilai pemahaman rata-rata 50%.   

Pengaruh Pengaturan Suhu dan Kelembaban di Kandang Closed House Terhadap Performa Broiler

Penelitian ini bertujuan untuk mengetahui pengaruh pengaturan suhu dan kelembaban terhadap performa broiler di kandang closed house pada pemeliharaan usia 15-30 hari.  Penelitian ini menggunakan 120 ekor broiler strain Cobb 500 berusia 15 hari, yang  dipelihara selama 15 hari yang disusun berdasarkan rancangan Rancangan Acak Lengkap (RAL) dengan 3 taraf perlakuan dan 4 kali ulangan.  Setiap unit percobaan terdiri dari 10 ekor broiler dengan perlakuan suhu dan kelembaban masing-masing 27 0C dan 88 %, 28 0C dan 87 % dan 29 0C dan 86 %.  Parameter yang diamati yaitu performa broiler yang terdiri dari konsumsi pakan, pertambahan bobot badan, feed convertion ratio (FCR), dan mortalitas. Hasil penelitian ini menunjukkan bahwa pengaturan suhu dan kelembaban memberikan pengaruh sangat nyata (P<0.01) terhadap konsumsi pakan, namun tidak berpengaruh nyata (P>0.05) terhadap pertambahan bobot dan Feed Convertion Ratio (FCR), mortalitas broiler selama penelitian sebesar 0.83%

INTRODUKSI TEKNOLOGI INSEMINASI BUATAN PADA UNGGAS LOKAL DI DESA BARAMBANG KECAMATAN SINJAI BORONG KABUPATEN SINJAI

Peningkatan mutu bibit ternak unggas di pedesaan tidak terlepas dari peran teknologi, salah satunya adalah teknologi reproduksi seperti inseminasi buatan (IB). Kegiatan Pengabdian kepada masyarakat (PkM) ini bertujuan untuk meningkatkan pengetahuan masyarakat Desa Barambang, Kecamatan Sinjai Borong, Kabupaten Sinjai mengenai introduksi teknologi inseminasi buatan pada unggas. Kegiatan dilakukan dengan empat tahapan, yaitu pembagian kuesioner pre test kepada peserta PkM, presentasi materi PkM, pelatihan IB pada ayam kampung dan pembagian kuesioner post test kepada peserta PkM. Data diolah secara deskriptif, independent sample-T test dan uji Mann-Whitney U. Sebagian besar peserta PkM memiliki pekerjaan petani, jumlah ayam yang dipelihara 1-5 ekor, sistem pemeliharaan unggas ekstensif dan tidak adanya pengontrolan perkawinan pada ayam.  Sebelum kegiatan PkM (pre test) seluruh peserta beum mengetahui manfaat dan tatacara IB pada unggas. Pengetahuan peserta PkM mengalami peningkatan setelah kegiatan (post test) dengan skor rata-rata pemahaman manfaat IB 35.92 dan skor rata-rata pemahaman tatacara IB 46.04. Peserta dengan kelompok umur 27-48 tahun dan pendidikan terakhir sekolah menengah lebih mampu menyerap materi PkM yang diberikan jika dibandingkan kelompok peserta dengan umur 49-70 tahun dan pendidikan terakhir sekolah dasar. Kesimpulan dari kegiatan PkM ini adalah introduksi teknologi IB pada unggas di Desa Barambang mampu meningkatkan pengetahuan masyarakat mengenai manfaat dan tatacara IB pada unggas, dimana masyarakat dengan umur produktif dan pendidikan yang lebih tinggi lebih mampu memahami pengetahuan yang diberikan

Basic science232. Certolizumab pegol prevents pro-inflammatory alterations in endothelial cell function

Background: Cardiovascular disease is a major comorbidity of rheumatoid arthritis (RA) and a leading cause of death. Chronic systemic inflammation involving tumour necrosis factor alpha (TNF) could contribute to endothelial activation and atherogenesis. A number of anti-TNF therapies are in current use for the treatment of RA, including certolizumab pegol (CZP), (Cimzia ®; UCB, Belgium). Anti-TNF therapy has been associated with reduced clinical cardiovascular disease risk and ameliorated vascular function in RA patients. However, the specific effects of TNF inhibitors on endothelial cell function are largely unknown. Our aim was to investigate the mechanisms underpinning CZP effects on TNF-activated human endothelial cells. Methods: Human aortic endothelial cells (HAoECs) were cultured in vitro and exposed to a) TNF alone, b) TNF plus CZP, or c) neither agent. Microarray analysis was used to examine the transcriptional profile of cells treated for 6 hrs and quantitative polymerase chain reaction (qPCR) analysed gene expression at 1, 3, 6 and 24 hrs. NF-κB localization and IκB degradation were investigated using immunocytochemistry, high content analysis and western blotting. Flow cytometry was conducted to detect microparticle release from HAoECs. Results: Transcriptional profiling revealed that while TNF alone had strong effects on endothelial gene expression, TNF and CZP in combination produced a global gene expression pattern similar to untreated control. The two most highly up-regulated genes in response to TNF treatment were adhesion molecules E-selectin and VCAM-1 (q 0.2 compared to control; p > 0.05 compared to TNF alone). The NF-κB pathway was confirmed as a downstream target of TNF-induced HAoEC activation, via nuclear translocation of NF-κB and degradation of IκB, effects which were abolished by treatment with CZP. In addition, flow cytometry detected an increased production of endothelial microparticles in TNF-activated HAoECs, which was prevented by treatment with CZP. Conclusions: We have found at a cellular level that a clinically available TNF inhibitor, CZP reduces the expression of adhesion molecule expression, and prevents TNF-induced activation of the NF-κB pathway. Furthermore, CZP prevents the production of microparticles by activated endothelial cells. This could be central to the prevention of inflammatory environments underlying these conditions and measurement of microparticles has potential as a novel prognostic marker for future cardiovascular events in this patient group. Disclosure statement: Y.A. received a research grant from UCB. I.B. received a research grant from UCB. S.H. received a research grant from UCB. All other authors have declared no conflicts of interes

Global economic burden of unmet surgical need for appendicitis

Background: There is a substantial gap in provision of adequate surgical care in many low-and middle-income countries. This study aimed to identify the economic burden of unmet surgical need for the common condition of appendicitis. Methods: Data on the incidence of appendicitis from 170 countries and two different approaches were used to estimate numbers of patients who do not receive surgery: as a fixed proportion of the total unmet surgical need per country (approach 1); and based on country income status (approach 2). Indirect costs with current levels of access and local quality, and those if quality were at the standards of high-income countries, were estimated. A human capital approach was applied, focusing on the economic burden resulting from premature death and absenteeism. Results: Excess mortality was 4185 per 100 000 cases of appendicitis using approach 1 and 3448 per 100 000 using approach 2. The economic burden of continuing current levels of access and local quality was US $92 492 million using approach 1 and$73 141 million using approach 2. The economic burden of not providing surgical care to the standards of high-income countries was $95 004 million using approach 1 and$75 666 million using approach 2. The largest share of these costs resulted from premature death (97.7 per cent) and lack of access (97.0 per cent) in contrast to lack of quality. Conclusion: For a comparatively non-complex emergency condition such as appendicitis, increasing access to care should be prioritized. Although improving quality of care should not be neglected, increasing provision of care at current standards could reduce societal costs substantially