Simultaneous quantification of Young’s modulus and dispersion forces with nanoscale spatial resolution

Many advances in polymers and layered materials rely on a precise understanding of the local interactions between adjacent molecular or atomic layers. Quantifying dispersion forces at the nanoscale is particularly challenging with existing methods often time consuming, destructive, relying on surface averaging or requiring bespoke equipment. Here, we present a non-invasive method able to quantify the local mechanical and dispersion properties of a given sample with nanometer lateral precision. The method, based on atomic force microscopy (AFM), uses the frequency shift of a vibrating AFM cantilever in combination with established contact mechanics models to simultaneously derive the Hamaker constant and the effective Young’s modulus at a given sample location. The derived Hamaker constant and Young’s modulus represent an average over a small (typically <100) number of molecules or atoms. The oscillation amplitude of the vibrating AFM probe is used to select the length-scale of the features to analyse, with small vibrations able to resolve the contribution of sub-nanometric defects and large ones exploring effectively homogeneous areas. The accuracy of the method is validated on a range of 2D materials in air and water as well as on polymer thin films. We also provide the first experimental measurements of the Hamaker constant of HBN, MoT2, WSe2 and polymer films, verifying theoretical predictions and computer simulations. The simplicity and robustness of the method, implemented with a commercial AFM, may support a broad range of technological applications in the growing field of polymers and nanostructured materials where a fine control of the van der Waals interactions is crucial to tune their properties

Force reconstruction from tapping mode force microscopy experiments

Fast, accurate, and robust nanomechanical measurements are intensely studied in materials science, applied physics, and molecular biology. Amplitude modulation force microscopy (tapping mode) is the most established nanoscale characterization technique of surfaces for air and liquid environments. However, its quantitative capabilities lag behind its high spatial resolution and robustness. We develop a general method to transform the observables into quantitative force measurements. The force reconstruction algorithm has been deduced on the assumption that the observables (amplitude and phase shift) are slowly varying functions of the tip–surface separation. The accuracy and applicability of the method is validated by numerical simulations and experiments. The method is valid for liquid and air environments, small and large free amplitudes, compliant and rigid materials, and conservative and non-conservative forces.This work was funded by the Spanish Ministry of Economy (MINECO) through grant CSD2010–00024 and the European Research Council ERC-AdG-340177 (3DNanoMech)

Opportunities and challenges for biosensors and nanoscale analytical tools for pandemics: COVID-19

Biosensors and nanoscale analytical tools have shown a huge growth in literature in the past 20 years, with a large number of reports on the topic of ’ultra-sensitive’, ’costeffective’ and ’early-detection’ tools with a potential of ’mass-production’ cited on the web of science. Yet none of these tools are commercially available in the market or practically viable for mass production and use in pandemic diseases such as COVID-19. In this context, we review the technological challenges and opportunities of current bio/chemical sensors and analytical tools by critically analyzing the bottlenecks which have hindered the implementation of advanced sensing technologies in pandemic diseases. We also describe in brief COVID-19 by comparing it with other pandemic strains such as SARS and MERS for the identification of features that enable biosensing. Moreover, we discuss visualization and characterization tools that can potentially be used not only for sensing applications but also assist in speeding up the drug discovery and vaccine development process. Furthermore, we discuss the emerging monitoring mechanism, namely wastewater-based epidemiology, for early warning of the outbreak, focusing on sensors for rapid and on-site analysis of SARS-COV-2 in sewage. To conclude, we provide holistic insights into challenges associated with the quick translation of sensing technologies, policies, ethical issues, technology adoption, and an overall outlook of the role of the sensing technologies in pandemics

Sensing Dynamically Evolved Short‐Range Nanomechanical Forces in Fast‐Mutating Single Viral Spike Proteins

Understanding changes in the mechanical features of a single protein from a mutated virus while establishing its relation to the point mutations is critical in developing new inhibitory routes to tackle the uncontrollable spread of the virus. Addressing this, herein, the chemomechanical features of a single spike protein are quantified from alpha, beta, and gamma variants of SARS-CoV-2. Integrated amplitude-modulation atomic force microscopy is used with dynamic force–distance curve (FDC) spectroscopy, in combination with theoretical models, to quantify Young's modulus, stiffness, adhesion forces, van der Waals forces, and the dissipative energy of single spike proteins. These obtained nanomechanical properties can be correlated with mutations in the individual proteins. Therefore, this work opens new possibilities to understand how the mechanical properties of a single spike protein relate to the viral functions. Additionally, single-protein nanomechanical experiments enable a variety of applications that, collectively, may build up a new portfolio of understanding protein biochemistry during the evolution of viruses