Effect of Chlorella vulgaris Alcoholic Extract to Limit the Growth of (Staphylococcus aurous) That Couse Food Poison

The present study was planned to investigate and conducted to evaluate the antibacterial activities of the green algae crud extract of Chlorella vulgaris Staphylococcal food poisoning is a common cause of foodborne illness worldwide. The growth phases of algae were determined and the cultures were harvested at the end of logarithmic phase .The cultures of green algae gave 0.2gm/L. The lipid content of C. vulgaris cell was measured and it was 6.3% of dry weight then the fatty acids were analyzed using the high perform liquid chromatography HPLC technique system. The pathogenic bacteria was isolated and identified from different parts of body including swab from skin, expectorant of lung and from sputum of gum , then 5 different concentration of crude extract of alga C. vulgaris, 100%, 50%,25%,12.5% and 6.3% were tested against pathogenic bacteria Staphylococcus aureus .The resistance of bacterial isolates was examined against all algal extract concentrations. The results showed that Staphylococcus aureus was sensitive to 6.3% concentration of the crude extract of alga C. vulgaris which is the lowest concentration of the algal crude extract. The study concluded that microalgae especially fresh water algae represent a very important source for many active compounds and can be used as antimicrobial agents

An efficient synthesis and spectroscopic characterization of Schiff bases containing 9,10-anthracenedione moiety

A new method has been developed for the synthesis of novel Schiff bases containg anthraquinone moiety using dodeca-Tungstosilicic acid/P2O5 under solvent free conditions at room temperature. The reaction was completed in 1-3 minutes with excellent yields. This method was found to be more efficient, easy and hazardous free for the synthesis of azomethines. The development of these type of methadologies in synthetic chemistry may contribute to green chemistry. The structures of synthesized novel Schiff bases was elucidated using 1H-NMR, 13C-NMR, LCMS, FTIR and CHN analysis

A lightweight and secure multilayer authentication scheme for wireless body area networks in healthcare system

Wireless body area networks (WBANs) have lately been combined with different healthcare equipment to monitor patients' health status and communicate information with their healthcare practitioners. Since healthcare data often contain personal and sensitive information, it is important that healthcare systems have a secure way for users to log in and access resources and services. The lack of security and presence of anonymous communication in WBANs can cause their operational failure. There are other systems in this area, but they are vulnerable to offline identity guessing attacks, impersonation attacks in sensor nodes, and spoofing attacks in hub node. Therefore, this study provides a secure approach that overcomes these issues while maintaining comparable efficiency in wireless sensor nodes and mobile phones. To conduct the proof of security, the proposed scheme uses the Scyther tool for formal analysis and the Canetti–Krawczyk (CK) model for informal analysis. Furthermore, the suggested technique outperforms the existing symmetric and asymmetric encryption-based schemes

E-government based on the blockchain technology, and the evaluation of its transaction through the number of transactions completed per second

Blockchain technology is one of the basic technologies for securing data sharing and storage across peer-to-peer systems in a distributed and untrusted network. Information is stored in electronic governance, which is considered sensitive data about citizens and companies and is the focus of external attacks. E-government has one point of failure and depends on centralization, and the decision is in the hands of one party or one official. Therefore, a secure and distributed electronic system for e-governance based on blockchain technology has been proposed. The system consists of several entities, organizations or nodes responsible for consensus to make decisions. Users are given the right to raise a transaction or send a request. The transaction is evaluated by auditors, and the citizen acquires a smart contract as a way out. The proposed system was compared with electronic governance systems without the use of blockchain technology. The proposed system was tested and compared with the previous systems, and it was found that the proposed system was superior in terms of security, the speed of processing the transaction and the time of filing the transaction

A Proposed hash algorithm to use for blockchain base transaction flow system

Blockchain technology introduces a new approach to storing information, implementing tasks and functions, and building trust between participating nodes. Although blockchain technology has received extensive attention in various application contexts in recent years, the issue of privacy and security remains the primary focus of discussions of the blockchain. The use of hash algorithms can provide secure blockchain integration, and many hash algorithms offer solutions to data integrity and security problems within the context of blockchain technology. However, they are also subject to problems related to time, lack of resources, and memory usage. In this research, an algorithm is proposed to generate a hash based on chaos theory (1D and 2D) logistic maps and the new Merkle-Damgård construction. Hash outputs are tested in terms of time, complexity, and collision. The proposed algorithm is evaluated according to Jaccard similarity and various coefficient measurements, and it was found that the similarity between the inputs and the outputs does not exceed 0.1932 percent. All outcomes indicated successful performance. The proposed algorithm was implemented on a blockchain-based transaction flow system, consumed fewer resources than other hash algorithms (such as SHA1, SHA2, and MD5), and requires mere milliseconds to implement

Data retrieval based on the smart contract within the blockchain

Blockchain technology appears to be the ideal solution for storing data in a transparent and decentralized manner. It also allows open access to data and enhances its immutable nature. This technology has helped prove its usefulness in several industries so far, however, distributed ledger technology does not work as a pure database. Therefore, some problems occur in accessing data. Querying data in the blockchain leads to performance and bandwidth problems. This primarily occurs because the blockchain does not have a primary query language, unlike regular databases. The distributed nature of the blockchain is in this case an obstacle. In this paper, a safe and fast method will be proposed to retrieve consistent data from the blockchain-based on the smart contract that will be opened after completing the transaction procedures. All nodes will sign the proposed transaction (by adding a special hash to each node resulting from the transaction information and node data). Upon completion of Transaction procedures, A smart contract will be opened (in which a QR is placed) resulting from converting the signatures in the transaction to QR When the smart contract data is retrieved, the QR for each transaction will be used All node signatures and transaction data will be extracted. The data will be retrieved by the QR generated for each transaction after it is stored in all nodes servers participating in the system. A new method was proposed to generate a hash for each node present in the system. The proposed method was tested in terms of time and complexity, and the algorithm was statistically analyzed, and all the results proved successful

Studies on uricase induction in certain bacteria

Three strains of Proteus vulgaris and two Streptomyces species were screened for inducible uricase formation. P. vulgaris (1753 and B-317-C), Streptomyces graminofaciens and S.albidoflavus showed inducible uricase activity, but P. vulgaris U7 did not show activity under the experimental conditions tested. Different amounts of constitutive and induced uricase were obtained by the four organisms using different culture media. The enzyme was induced in the producing organisms by different concentrations of different inducers, and uric acid was the mostpotent inducer. Using the optimal concentration of uric acid as inducer, the conditions of uricase induction in the test organisms were optimized. In P. vulgaris strains (1753 and B-317-C), theincubation temperature of 37 ºC, initial pH of culture media of 7 and agitation rate of 180 rpm, showed the highest level of uricase induction. In the two Streptomyces species, the uricase induction was optimized at 28 ºC incubation temperature and pH 7. The agitation rate of 200 and 220 rpm showed the highest induction activity in Streptomyces graminofaciens and S. albidoflavus, respectively. The highest levels of induced uricase were obtained at induction times of 140 min, 140 min, 42 h and 36 h in P. vulgaris 1753, B-317-C, Streptomyces graminofaciens and S. albidoflavus, respectively. The uricase was present as cell-bound enzyme in the producing organisms and no activity was recorded in the culture supernatants

Synthesis, spectroscopic characterization and pharmacological evaluation of oxazolone derivatives

A series of 4-aryl methylidene-2-phenyl/methyl-5-(4H)-oxazolone derivatives (2-7) have been synthesized using the reported method by condensation of aldehydes with N-benzoyl / N-acetyl glycine in the presence of zinc oxide as a catalyst and acetic anhydride at room temperature in ethanol. The compounds (2-6) are new derivatives. The structures of compounds were evaluated on the basis of 1H-NMR, 13C-NMR, EIMS, FT-IR and elemental analysis. All the compounds were screened for their antibacterial and urease inhibition activity. Antibacterial activity was tested by agar well diffusion method using Mueller Hinton Agar medium. Compound (2) showed excellent activity against S. aureus which has 16 mm (80%) inhibition and above 24 mm (70%) against S. typhi. The most active compound against E. coli was compound (6) having 20 mm (80%) inhibition followed by compound (5) having above 18 mm (70%) inhibition. Urease inhibition activity of all the compounds was determined by indophenol method. Compounds (3, 6) and (7) showed significant inhibition against Jacks bean urease

Kernohan–woltman notch phenomenon:Case report and Review of literature

Subdural hematoma (SDH) is usually secondary to mechanical trauma. Spontaneous subdural hematoma is still rare with unclear pathophysiology. The classic findings of subdural hematoma is sudden drop in mental status, an ipsilateralmydriasis with contralateral hemiparesis. However SDH can sometimes compress the contralaterl corticospinal tract resulting in ipsilateral motor weakness, this phenomenon is known as Kernohan-Woltman Notch Phenomenon

Sero-prevalence of Human Cytomegalovirus among blood donors in Lahore, Pakistan

Background: Transfusion-transmitted cytomegalovirus (TT-CMV) infection can cause severe illness and even death among immunocompromised patients; therefore, the spread of CMV through blood products should be prevented. To our knowledge, no study has been carried out in Pakistan to determine the seroprevalence of CMV in general population as well as among blood donors. The goal of this study was to determine CMV seropositivity among blood donors at the blood bank of INMOL Hospital, Lahore, Pakistan.Methods: A sero-epidemiological cross-sectional study was conducted. Sera from 91 blood donors were screened for CMV specific IgG antibodies by enzyme-linked immunosorbent assay (ELISA) based kit.Results: The CMV-specific IgG antibodies were detected in 89 blood donors, which gave seroprevalence rate of 97.8%. The statistical analysis of results was done using pearson chi-square test and appeared non-significant with values 0.625 and 0.705 for different age groups and blood groups of donors.Conclusion: Because of high seroprevalence in this study area, an adequate supply of CMV seronegative blood is difficult to maintain. Therefore, we propose that the future strategies for the prevention of post-transfusion CMV infection in recipients should include the transfusion of leukoreduced blood products. Further a prospective study with much greater population can be done to identify major causative risk factors for such highest prevalence rate