Polychlorinated Biphenyl Levels and its Correlation to Size of Marine Organisms Harvested from a War-Induced Oil Spill Zone of the Eastern Mediterranean Sea

This is the first work establishing a base-line data of the level of total Polychlorinated Biphenyl (PCB) contaminants in selected marine organisms (Siganus rivulatus, Mullet spp., and oysters) and its relationship to organism size and the harvest distance from the oil spill source. Six locations across the Lebanese Mediterranean were included for sampling. Oysters and the two fish types were collected after 72 days of the spill. The length, maximum width, and whole weight of individual organisms were recorded. Methanol extracts of the samples were analyzed for total PCB using a Competitive Enzyme-linked Immunosorbent Assay (ELISA) based Spectrophotometry.All means of PCB in the three selected marine organisms were below the guidance level set by USFDA (2 ppm). A total of 6 significant regression equations were established between the total PCB level and certain size dimensions of specific selected marine species, with values of R2 ranging between 0.719 – 0.909 and P values ranging from 0.038 – 0.099.In addition, the total PCB level in Siganus rivulatus correlated with the harvest distance north of the oil spill source, signifying a drop in total PCB level with an increase in harvest distance from the oil spill source

A Nexus Approach for the MENA Region—From Concept to Knowledge to Action

There is wide agreement that a nexus or integrated approach to managing and governing natural resources such as land, water, and energy can improve environmental, climate, human, and political security. However, few if any countries in the MENA region have made progress in implementing such an approach. There appear to be several constraints inhibiting the development and adoption of nexus approaches. These constraints include strong sectoral silos, insufficient incentives for integrated planning and policy making at all levels, and limited vision, knowledge, and practical experience to guide successful implementation. In turn, the limited implementation and hence lack of empirical evidence of a nexus approach, which could demonstrate its benefits, does little to strengthen political will for the development of adequate incentives, structures, and procedures. Against this backdrop, this paper presents five case studies which take an integrated approach, in three MENA countries, namely Jordan, Lebanon, and Morocco. Based on an analytical framework developed here, the paper analyses and compares the success factors for nexus implementation, and also for transfer and upscaling. The analysis emphasizes the need for appropriate framework conditions, targeted investments and pioneering actors, to make integrated approaches across sectors and levels work. With the evidence presented, the paper aims to set in motion a positive or virtuous cycle of generating more nexus evidence, improved framework conditions, further nexus implementation on the ground, and from that even more nexus evidence. Finally, the paper contributes to overcoming the repeated requests for better definition and conceptualization of the nexus, which often has slowed down adoption of the concept

Reference genes for gene expression studies in wheat flag leaves grown under different farming conditions

<p>Abstract</p> <p>Background</p> <p>Internal control genes with highly uniform expression throughout the experimental conditions are required for accurate gene expression analysis as no universal reference genes exists. In this study, the expression stability of 24 candidate genes from <it>Triticum aestivum </it>cv. Cubus flag leaves grown under organic and conventional farming systems was evaluated in two locations in order to select suitable genes that can be used for normalization of real-time quantitative reverse-transcription PCR (RT-qPCR) reactions. The genes were selected among the most common used reference genes as well as genes encoding proteins involved in several metabolic pathways.</p> <p>Findings</p> <p>Individual genes displayed different expression rates across all samples assayed. Applying geNorm, a set of three potential reference genes were suitable for normalization of RT-qPCR reactions in winter wheat flag leaves cv. Cubus: <it>TaFNRII </it>(ferredoxin-NADP(H) oxidoreductase; AJ457980.1), <it>ACT2 </it>(actin 2; TC234027), and <it>rrn26 </it>(a putative homologue to RNA 26S gene; AL827977.1). In addition of these three genes that were also top-ranked by NormFinder, two extra genes: <it>CYP18-2 </it>(Cyclophilin A, AY456122.1) and <it>TaWIN1 </it>(14-3-3 like protein, AB042193) were most consistently stably expressed.</p> <p>Furthermore, we showed that <it>TaFNRII, ACT2</it>, and <it>CYP18-2 </it>are suitable for gene expression normalization in other two winter wheat varieties (Tommi and Centenaire) grown under three treatments (organic, conventional and no nitrogen) and a different environment than the one tested with cv. Cubus.</p> <p>Conclusions</p> <p>This study provides a new set of reference genes which should improve the accuracy of gene expression analyses when using wheat flag leaves as those related to the improvement of nitrogen use efficiency for cereal production.</p

Position Paper on Water, Energy, Food and Ecosystem (WEFE) Nexus and Sustainable development Goals (SDGs)

The EU and the international community is realising that the Water, Energy, Food and Ecosystem components are interlinked and require a joint planning in order to meet the daunting global challenges related to Water, Energy and Food security and maintaining the ecosystem health and in this way, reach the SDGs. If not dealt with, the world will not be able to meet the demand for water, energy and food in a not too far future and, in any case, in a not sustainable way. The strain on the ecosystems resulting from unsustainable single-sector planning will lead to increasing poverty, inequality and instability. The Nexus approach is fully aligned with and supportive of the EU Consensus on Development. Key elements of the Consensus will require collaborative efforts across sectors in ways that can be supported/implemented by a Nexus approach. In this way, transparent and accountable decision-making, involving the civil society is key and common to the European Consensus on Development and the Nexus approach. The Nexus approach will support the implementation of the SDG in particular SDG 2 (Food), SDG 6 (Water) and SDG 7 (Energy), but most SDGs have elements that link to food, water and energy in one or other way, and will benefit from a Nexus approach. The SDGs are designed to be cross-cutting and be implemented together, which is also reflected in a WEFE Nexus approach. A Nexus approach offers a sustainable way of addressing the effects of Climate Change and increase resilience. The WEFE Nexus has in it the main drivers of climate change (water, energy and food security) and the main affected sectors (water and the environment). Decisions around policy, infrastructure, … developed based on the WEFE Nexus assessments will be suitable as elements of climate change mitigation and adaptation. In fact, it is difficult to imagine solutions to the climate change issue that are not built on a form of Nexus approach. The Nexus approach is being implemented around the world, as examples in the literature demonstrate. These examples together with more examples from EU and member state development cooperation will help build experience that can be consolidated and become an important contribution to a Toolkit for WEFE Nexus Implementation. From the expert discussions, it appears that because of the novelty of the approach, a Toolkit will be an important element in getting the Nexus approach widely used. This should build on experiences from practical examples of NEXUS projects or similar inter-sectorial collaboration projects; and, there are already policy, regulation and practical experience to allow institutions and countries to start applying the Nexus concept.JRC.D.2-Water and Marine Resource

Educational, institutional and governmental obstacles to protecting water resources from improper agricultural practices in Lebanon

Agriculture is the biggest use of water in Lebanon, a relatively water-rich country in a mostly arid region. Pollution of water resources in Lebanon varies according to region but most is mainly due to sewage discharge and agricultural activities. As with intensive agricultural practices worldwide, the agricultural sector in Lebanon relies heavily on agrochemicals to protect crops and improve their yield. To ensure sustainable agricultural production, proper management of soil and water quality is required. This study aimed to identify obstacles (educational, institutional, regulatory, etc.) hindering the protection of water resources from inappropriate agricultural practices in Lebanon. First, a limited field survey was conducted to determine current farm practices with respect to the farmers’ awareness of the environmental impact of their practices, specifically agrochemical use, and the role of best management practices (BMPs). Second, a review of studies on the impact of agriculture on water resources in Lebanon and a review of current regulations and legislation aimed at controlling environmental degradation were performed. Finally, key barriers to implementing water resources protection measures (e.g. BMPs) were identified and measures to over them recommended