Effects of combination of ethylenediaminetetraacetic acid and microbial phytase on the serum concentration and digestibility of some minerals in broiler chicks

This experiment was conducted to evaluate the combined effects of ethylene diamine tetraacetic acid (EDTA) and microbial phytase (MP) on the serum concentration and digestibility of some minerals in broiler chicks. This experiment was conducted using 360 Ross-308 male broiler chicks in a completely randomized design with a 3×2 factorial arrangement (0, 0.1 and 0.2% EDTA and 0 and 500 FTU MP). Fourreplicates of 15 chicks per each were fed dietary treatments which included; P-deficient basal diet [0.2% available phosphorus (aP)] (NC), NC + 500 FTU MP per kilogram of diet, NC + 0.1% EDTA per kilogram ofdiet, NC + 0.1% EDTA + 500 FTU MP per kilogram of diet, NC + 0.2% EDTA per kilogram and NC + 0.2% EDTA + 500 FTU MP per kilogram of diet. The concentration of zinc, copper and manganese of serum and their digestibility and also digestibility of apparent metabolizable energy (AMEn) was evaluated. The results showed that phytase supplementation of P-deficient diets significantly increased zinc concentration of serum (P < 0.05). Interaction effect of EDTA+MP on serum concentration of copper and manganese and also digestibility of zinc was significant (P < 0.05). EDTA supplementation of P-deficient diets significantly increased manganese digestibility in broiler chicks (P < 0.01). Keywords: Ethylene diamine tetraacetic acid, microbial phytase, zinc, copper, manganes

Modeling surface vibrations and their role in molecular adsorption: a generalized Langevin approach

The atomic vibrations of a solid surface can play a significant role in the reactions of surface-bound molecules, as well as their adsorption and desorption. Relevant phonon modes can involve the collective motion of atoms over a wide array of length scales. In this manuscript, we demonstrate how the generalized Langevin equation can be utilized to describe these collective motions weighted by their coupling to individual sites. Our approach builds upon the generalized Langevin oscillator (GLO) model originally developed by Tully \textit{et al.} We extend the GLO by deriving parameters from atomistic simulation data. We apply this approach to study the memory kernel of a model platinum surface and demonstrate that the memory kernel has a bimodal form due to coupling to both low-energy acoustic modes and high-energy modes near the Debye frequency. The same bimodal form was observed across a wide variety of solids of different elemental compositions, surface structures, and solvation states. By studying how these dominant modes depend on simulation size, we argue that the acoustic modes are frozen in the limit of macroscopic lattices. By simulating periodically replicated slabs of various sizes we quantify the influence of phonon confinement effects in the memory kernel and their concomitant effect on simulated sticking coefficients

Evaluation of yield, yield components and nutritive value in intercropping of Barley with Vetch

To evaluate some agronomic properties and forage characteristics in the intercropping of barley (Hordeum vulgare L.) and vetch (Vicia ervilia L.), an experiment was conducted during 2014-2016 cropping seasons. A randomized complete block design with four replications was used. Intercropping patterns included 80% barley+ 20% vetch, 60% barley+ 40% vetch, 40% barley + 60% vetch and 20% barley + 80% vetch along with the sole culture of both crops (100% barley and100% vetch). The result showed that the highest value of plant height, grain number, thousand-grain weight, biological yield, grain yield and harvest in barley was observed from 80% barley + 20% vetch intercropping ratio, however, for vetch, it was detected from 100% vetch. Furthermore, the highest land equivalent ratio was obtained from 80% barley+ 20% vetch. Based on the results, the highest crude protein content and dry matter digestibility were observed in sole cropped vetch, whereas the highest neutral detergent fiber and acid detergent fiber were recorded in sole cropped barley. These results suggested that intercropped barley and vetch as 80% barley + 20% vetch ameliorated the grain yield and yield components, and forage quality compared to other intercropping ratios

The effect of bio-fertilizers and a super-absorbent on the quantitative and qualitative characteristics of castor bean (Ricinus communis L.) under water-deficit conditions

The effect of bio-fertilizers and super-absorbents on the modification of water-deficit stress was studied in castor. A field experiment was conducted with a split-plot design based on a completely randomized block design with three replications. The first factor included four levels (70, 100, 130, and 160 mm of evaporation pan A Class) in the main plot; the sub-factor was the application of four bio-fertilizer treatments (Azospirillum, Citrobacter, Azospirillum + Citrobacter, and control); and the superabsorbent at two levels (superabsorbent consumption and control) as a factorial was placed in the sub-plots. With the intensification of the water-deficit stress from 70 to 160 mm of evaporation, the number of seeds per plant, the 1,000-kernel weight, and the oil percentage significantly dropped. All three bio-fertilizer treatments significantly increased the number of grains, 1,000 kernel weight, and oil percentage compared to the control. Citrobacter in all four irrigation levels significantly increased the content of photosynthetic pigments, grin yield, oil yield and proline content and decreased the catalase activity and malondialdehyde content. The application of the super-absorbent under the stress condition of 160 mm significantly raised the content of chlorophyll b, soluble sugar, grain yield, oil yield, and proline compared to the control treatment. Among the combinations of bio-fertilizer and super-absorbent treatments, the highest photosynthetic pigments’ content, grain yield, and oil yield and the lowest catalase enzyme activity belonged to Azospirillum + superabsorbent consumption. However, the maximum soluble sugars and the minimum malondialdehyde content belonged to Citrobacter + superabsorbent

Tecnología de semillas sintéticas para la encapsulación y el recrecimiento de puntas de brotes y embriones somáticos de Espárrago officinalis L

Apical buds obtained from Asparagus plant vitro culture and somatic embryos obtained from stem cultivation, explants in MS medium supplemented with mg¹ᶫ 1, 2, 4-D and mg¹ᶫ 1 and Kinetin have been used in this research to produce artificial seeds. We encapsulated apical buds and somatic embryo using 2% sodium alginate and calcium chloride to prepare the artificial seeds. We placed artificial seeds at room temperature (about 25 ° C), in the cold, the temperature of 4 ° C and -18 ° C for different times (15,30,60,90 days) and evaluated the growing power of these seeds in MS and ½MS mediums for further investigations about the viability of seeds. The highest conversion percentage of seedlings in encapsulated embryos (70.01) was related to seed harvested from embryos treated with BA and the highest conversion percentage of seedlings in apical buds (96.54) was obtained from cultivated untreated seeds in MS medium. Encapsulated arteries and buds maintained germination energy and viability with increasing storage time after 90 days of storage at 4 and 25 ° C despite viability reduction while un-capsulated embryos and buds completely lost viability after 60 days of storage at 4 and 25 ° C and seeds stored at -18 ° C completely lost viability after 15 days of storage. In general, the percentage of seed germination and conversion to seedling is higher in seeds cultivated in MS medium compared to seeds cultivated in ½MS medium.En esta investigación se han utilizado yemas apicales obtenidas de cultivo vitro vegetal de espárragos y embriones somáticos obtenidos del cultivo de tallos, explantes en medio MS suplementado con mg¹ᶫ 1, 2, 4-D y mg¹ᶫ 1 y Kinetin para producir semillas artificiales. Encapsulamos yemas apicales y embriones somáticos utilizando alginato de sodio al 2% y cloruro de calcio para preparar las semillas artificiales. Colocamos semillas artificiales a temperatura ambiente (alrededor de 25 ° C), en el frío, la temperatura de 4 ° C y -18 ° C para diferentes tiempos (15,30,60,90 días) y evaluamos el poder de crecimiento de estas semillas. en medios MS y ½MS para futuras investigaciones sobre la viabilidad de las semillas. El mayor porcentaje de conversión de plántulas en embriones encapsulados (70.01) se relacionó con la semilla recolectada de embriones tratados con BA y el mayor porcentaje de conversión de plántulas en yemas apicales (96.54) se obtuvo de semillas cultivadas sin tratar en medio MS. Las arterias y las yemas encapsuladas mantuvieron la energía de germinación y la viabilidad con un mayor tiempo de almacenamiento después de 90 días de almacenamiento a 4 y 25 ° C a pesar de la reducción de la viabilidad, mientras que los embriones y las yemas no encapsulados perdieron completamente la viabilidad después de 60 días de almacenamiento a 4 y 25 ° C y las semillas almacenado a -18 ° C perdió completamente la viabilidad después de 15 días de almacenamiento. En general, el porcentaje de germinación de semillas y conversión a plántula es mayor en semillas cultivadas en medio MS en comparación con semillas cultivadas en medio ½MS

Tecnología de semillas sintéticas para la encapsulación y el recrecimiento de puntas de brotes y embriones somáticos de Espárrago officinalis L

Apical buds obtained from Asparagus plant vitro culture and somatic embryos obtained from stem cultivation, explants in MS medium supplemented with mg¹ᶫ 1, 2, 4-D and mg¹ᶫ 1 and Kinetin have been used in this research to produce artificial seeds. We encapsulated apical buds and somatic embryo using 2% sodium alginate and calcium chloride to prepare the artificial seeds. We placed artificial seeds at room temperature (about 25 ° C), in the cold, the temperature of 4 ° C and -18 ° C for different times (15,30,60,90 days) and evaluated the growing power of these seeds in MS and ½MS mediums for further investigations about the viability of seeds. The highest conversion percentage of seedlings in encapsulated embryos (70.01) was related to seed harvested from embryos treated with BA and the highest conversion percentage of seedlings in apical buds (96.54) was obtained from cultivated untreated seeds in MS medium. Encapsulated arteries and buds maintained germination energy and viability with increasing storage time after 90 days of storage at 4 and 25 ° C despite viability reduction while un-capsulated embryos and buds completely lost viability after 60 days of storage at 4 and 25 ° C and seeds stored at -18 ° C completely lost viability after 15 days of storage. In general, the percentage of seed germination and conversion to seedling is higher in seeds cultivated in MS medium compared to seeds cultivated in ½MS medium.En esta investigación se han utilizado yemas apicales obtenidas de cultivo vitro vegetal de espárragos y embriones somáticos obtenidos del cultivo de tallos, explantes en medio MS suplementado con mg¹ᶫ 1, 2, 4-D y mg¹ᶫ 1 y Kinetin para producir semillas artificiales. Encapsulamos yemas apicales y embriones somáticos utilizando alginato de sodio al 2% y cloruro de calcio para preparar las semillas artificiales. Colocamos semillas artificiales a temperatura ambiente (alrededor de 25 ° C), en el frío, la temperatura de 4 ° C y -18 ° C para diferentes tiempos (15,30,60,90 días) y evaluamos el poder de crecimiento de estas semillas. en medios MS y ½MS para futuras investigaciones sobre la viabilidad de las semillas. El mayor porcentaje de conversión de plántulas en embriones encapsulados (70.01) se relacionó con la semilla recolectada de embriones tratados con BA y el mayor porcentaje de conversión de plántulas en yemas apicales (96.54) se obtuvo de semillas cultivadas sin tratar en medio MS. Las arterias y las yemas encapsuladas mantuvieron la energía de germinación y la viabilidad con un mayor tiempo de almacenamiento después de 90 días de almacenamiento a 4 y 25 ° C a pesar de la reducción de la viabilidad, mientras que los embriones y las yemas no encapsulados perdieron completamente la viabilidad después de 60 días de almacenamiento a 4 y 25 ° C y las semillas almacenado a -18 ° C perdió completamente la viabilidad después de 15 días de almacenamiento. En general, el porcentaje de germinación de semillas y conversión a plántula es mayor en semillas cultivadas en medio MS en comparación con semillas cultivadas en medio ½MS

Heritability of physiological traits of barley (Hordeum Vulgare L.) under salinity stress

Background: In this paper, to determine the genetic parameters and heritability of physiological traits related to salinity stress in barley (Hordeum Vulgare L.), a 7×7 half diallel cross was conducted at Islamic Azad University, Ardabil, during 2016-2017.Methods: F1 seeds are used in this experiment. These seeds have been grown under non-stress conditions, 8 and 12 dS/ m salinity stress levels, in a greenhouse alongside the parent plants. After exposure of plants to salinity, Leaf chlorophyll content, Chlorophyll Fluorescence and Stomatal Conductance were measured.Results: Narrow sense heritability of chlorophyll content ranged from 0.29 to 0.40. These values for chlorophyll fluorescence ranged from 0.16 to 0.24 and for stomatal conductance ranged from 0.26 to 0.54. Broad sense heritability was high among the evaluated traits and ranged from 0.70 to 0.87. The degree of average dominance was higher than one in all of traits.Conclusion: This observation indicated some degree of over dominance in control of the traits. Although dominant alleles were not observed in leaf chlorophyll content and chlorophyll fluorescence, yet they were favorable in stomatal conductance.Keywords: Salinity tension; Diallel; Heritability; Physiological traits

Evaluate the effect of salicylic acid and ascorbic acid on agronomic and antioxidant activities of Sunflower in water deficit conditions

To evaluate the effect of salicylic acid and ascorbic acid foliar application on a number of agronomic and antioxidant activities of sunflower, an experiment was conducted at research farm of Mahabad city, locted in the south of West Azerbaijan Province, Iran during 2016-2018 cropping seasons. The experiment was laid out as a split-plot design based on a completely randomized block design with three replications. Irrigation regimes (irrigation after 60 mm and irrigation after 120 mm evaporation from class A pan respectively) were established as main plots and the foliar applications of ascorbic acid (control, 50 and 100 ppm) and salicylic acid (control, 100 and 200 ppm) were the sub-plots. In the present study, the highest amount of proline content and activity of antioxidant enzymes was recorded under water deficit conditions and foliar application of 100 ppm salicylic acid. Applying salicylic acid increased grain yield under both normal irrigation and water deficit conditions. Also, the highest proline content and activity of antioxidant enzymes were under the treatment of water deficit and application of 50 ppm ascorbic acid. The results showed no significant difference between the combined foliar application of salicylic acid and ascorbic acid in terms of grain yield and oil percentage with their separate application. Therefore, simultaneous use of these two substances under normal irrigation and water deficit conditions was not economical and not recommended

Estimation of genetic parameters in three commercial silk-worm lines of Iran

In order to estimate genetic parameters of the cocoon weight (CW), cocoon shell weight (CSW) and cocoon shell percentage (CSP) of three industrial lines of silk-worm (number 31, 103 and 107), data were collected from three successive generations. (Co)variance components were as follows: additive genetic variation (δ2g) and environmental variation (δ2e) of traits and additive genetic covariance (covg1, 2) and environmental covariance (cove1, 2). Heritability of traits was estimated with maximum likelihood procedure (REML) by using algorithms (DFREML) based on a three trait animal model. Data were from half-sibs and the heritability and genetic, phenotypic and environmental correlation were estimated between traits. The highest heritability for CW was estimated in lines 31 and 107 (0.58 and 0.58, respectively) and the lowest heritability for CW was in line number 103 (0.28). The highest and lowest heritability for CSW was in lines 107 and 103 (0.70 and 0.01, respectively). Also, the highest heritability for CWP was in line 31 (0.48) and the lowest value was in line 103 (0.077). The highest δ2g for CSP was estimated in line 103 (0.004) and lowest δ2g was estimated in line 107 (0.001). The highest and lowest δ2g for CW were estimated in lines 103 and 31 (0.0004 and 0.00003, respectively). The δ2g for CW was high inline 31 (0.604) but low in line 103 (0.257). The highest and lowest δ2e for CW were in lines 103 and 107 (0.02 and 0.01, respectively). Furthermore, the highest δ2e for CSW was estimated in line 103 (0.0013) butminimum δ2e was in line 107 (0.0007). Maximum covg1, 2 for CW-CSW was estimated in line 103 (0.00087) and minimum value was estimated in line 31 (0.00012). A high cove1,2 for CW- CSP was estimated in line 103 (0.093) but it was low in line 31 (0.00026). The highest covg1, 2 for CSW-CSP was in line 103 (0.002) but it was low in line 31 (0.00079).Key words: Cocoon, correlation, heritability, genetic (Co) variance

Association between sleep duration and hypertension incidence: Systematic review and meta-analysis of cohort studies

Aim: Sleep duration has been suggested to be associated with hypertension (HTN). However, evidence of the nature of the relationship and its direction has been inconsistent. Therefore, we performed a meta-analysis to assess the relationship between sleep duration and risk of HTN incidence, and to distinguish more susceptible populations. Methods: PubMed, Embase, Scopus, Web of Science, and ProQuest were searched from January 2000 to May 2023 for cohort studies comparing short and long sleep durations with 7–8 hours of sleep for the risk of HTN incidence. Random-effect model (the DerSimonian-Laird method) was applied to pool risk ratios (RR) and 95% confidence interval (CI). Results: We included sixteen studies ranging from 2.4 to 18 years of follow-up duration evaluating HTN incidence in 1,044,035 people. Short sleep duration was significantly associated with a higher risk of developing HTN (HR: 1.07, 95% CI: 1.06–1.09). The association was stronger when the sleep duration was less than 5 hours (HR: 1.11, 95% CI: 1.08–1.14). In contrast to males, females (HR: 1.07, 95% CI: 1.04–1.09) were more vulnerable to developing HTN due to short sleep duration. No significant difference between different follow-up durations and age subgroups was observed. Long sleep duration was not associated with an increased incidence of HTN. Conclusion: Short sleep duration was associated with higher risk of HTN incidence, however, there was no association between long sleep duration and incidence of HTN. These findings highlight the importance of implementing target-specific preventive and interventional strategies for vulnerable populations with short sleep duration to reduce the risk of HTN